Ran qPCR on DNased RNA from earlier today to assess whether there was any residual gDNA after the DNase treatment with Oly_Actin_F/R primers (SR IDs: 1505, 1504).
Used 1μL from all templates.
All samples were run in duplicate.
Positive control was HL1 O.lurida DNA isolated by Jake on 20150323.
Cycling params:
95C – 2.5mins
40 cycles of:
95C – 10s
60C – 20s
Melt curve
Master mix calcs are here (used same calcs from the other day): 20150512_qPCR_Oly_RNA
Plate layout: 20150514_qPCR_plate_Jake_Oly_1hr_HS_DNased_RNA
Results:
qPCR Data File (Opticon): Sam_20150514_170332.tad
qPCR Report (Google Spreadsheeet): 20150514_qPCR_Report_Jake_Oly_DNased_1hr_HS_RNA
Positive control samples are the only samples that produced amplification (cycle ~20). Will proceed to making cDNA.
Amplification Plots
(http://eagle.fish.washington.edu/Arabidopsis/20150514_qPCR_Amp_DNased_RNA_Jake_Oly_1hr_HS.JPG)
Melt Curves
(http://eagle.fish.washington.edu/Arabidopsis/20150514_qPCR_Melt_DNased_RNA_Jake_Oly_1hr_HS.JPG)