Our sequencing data (Illumina HiSeq2500, 100SE) for this project has completed by Univ. of Oregon Genomics Core Facility (order number 2212).
Samples sequenced/pooled for this run:
| Sample | Treatment | Barcode |
| 400ppm | 400ppm | GCCAAT |
| 1000ppm | 1000ppm | CTTGTA |
All code listed below was run on OS X 10.9.5
Ran a bash script called “download.sh” to download all the files. The script contents were:
#!/bin/bash curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_001.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_002.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_003.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_004.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_005.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_006.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_007.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_008.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_009.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_010.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_011.fastq.gz curl -O https://gcf.uoregon.edu:8080/job/download/2212?fileName=lane2_NoIndex_L002_R1_012.fastq.gz
Downloaded all 12 fastq.gz files to Owl/web/nightingales/C_gigas
Renamed all files by removing the beginning of each file name (2112?fileName=) and replacing that with 2212_:
$for file in 2212*lane2_NoIndex_L002_R1_0*; do mv "$file" "${file/#2212?fileName=/2212_}"; done
Created a directory readme.md (markdown) file to list & describe directory contents: readme.md
$ls *.gz >> readme.md
Note: In order for the readme file to appear in the web directory listing, the file cannot be all upper-case.
Create MD5 checksums for each the files: checkums.md5
$md5 2212* >> checksums.md5