Continued Illumina library prep of bisulfite-treated DNA samples (400ppm and 1000ppm; from [20150114)(2015/01/14/dna-bisulfite-conversion-c-gigas-larvae-oa-sheared-dna.html) with Methylamp DNA Modification Kit (Epigentek). Performed bead clean up immediately after End Repair.
PCR cycles: 14
No other changes were made to the manufacturer’s protocol.
Epigentek Barcode Indices assigned, per their recommendations for using two libraries for multiplexing:
400ppm - barcode #6 - GCCAAT
1000ppm - barcode #12 - CTTGTA
The two libraries were stored @ -20C and will be quantified tomorrow.