RAD Sequencing - Oly Oyster gDNA-01 RAD Library (from 20141110)

2014
Olympia oyster reciprocal transplant
Samples Submitted
Author

Sam White

Published

November 17, 2014

Prepared 10nM of the library in total volume of 20uL of Buffer EB (Qiagen) + 0.1% Tween-20, according the University of Oregon’s Genomic’s Core:

https://gcf.uoregon.edu/home/services

Based on a calculator provided by their site, 10nM was equivalent to 2.93ng/uL. Diluted 3.13uL of the library (18.7ng/uL) in 16.87uL of Buffer EB + Tween 20.

Shipped FedEx Standard Overnight on wet ice for 100bp single end Illumina sequencing.