DNA Isolation - Mackenzie’s C.gigas Gonad Sample

2014
Miscellaneous
Author

Sam White

Published

April 1, 2014

Mac’s been having some difficulty getting good quality gDNA from some of her gonad samples, so she asked me to give it a shot.

Isolated DNA from 10mg (0.010g) of C.gigas gonad tissue using the Qiagen Blood & Tissue DNeasy Kit, with the following changes:

  1. Incubated sample in Buffer ATL + Proteinase K @ 56C for 3hrs

  2. Eluted sample in 100uL of Buffer AE.

Spec’d on NanoDrop1000.

Results:

DNA looks good, both 260/280 ratio and yield. The 260/230 ratio isn’t perfect, but it’s much better than what Mac was seeing. After showing her this, she’s decided to have me isolate DNA from the rest of her samples.