ePCR SOLiD Libraries - Yellow perch PQ, WB and Lake Trout Lean libraries (from 20100408)

2010
Miscellaneous
Author

Sam White

Published

April 13, 2010

ePCR was performed for the above three mentioned SOLiD libraries using 1.5pM (180 pg/uL) of cDNA, according to the ABI “full scale” ePCR protocol. ePCRs were stored @ 4C until ready for the emulsion breaking step.

Amounts of cDNA used to make dilutions (in 1x Low TE Buffer) of 180pg/uL:

PQ (20.77ng/uL): 4.33uL in 500uL

WB (16.81ng/uL): 2.14uL in 200uL

Lean (53.49ng/uL): 1.68uL in 500uL