qPCR was set up on these cDNAs using the following primers:
FP008495.p.cg.6 (“GSTO1”, “Glutathione S-transferase omega-1”) - This was upregulated in BB SOLiD data.
These were run in duplicate to take up a full PCR plate. qPCR set up and plate layout can be found here.
Results:
qPCR Data File (Opticon): 20091231_152520.tad
Data workup: PROPS BB_DH Gene Expression Miner Workup