SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245605_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 6684). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245605_1.fastq.gz Processing single-end reads on 1 core ... Finished in 3654.157 s (56.201 µs/read; 1.07 M reads/minute). === Summary === Total reads processed: 65,019,432 Reads with adapters: 25,103,690 (38.6%) Reads written (passing filters): 65,019,432 (100.0%) Total basepairs processed: 9,752,914,800 bp Quality-trimmed: 8,505,753 bp (0.1%) Total written (filtered): 9,675,071,385 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 25103690 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.8% C: 1.7% G: 12.8% T: 48.4% none/other: 0.3% Overview of removed sequences length count expect max.err error counts 1 20078057 16254858.0 0 20078057 2 2892686 4063714.5 0 2892686 3 1117640 1015928.6 0 1117640 4 489830 253982.2 0 489830 5 11043 63495.5 0 11043 6 9441 15873.9 0 9441 7 8558 3968.5 0 8558 8 8692 992.1 0 8692 9 9703 248.0 0 9188 515 10 8285 62.0 1 7070 1215 11 8846 15.5 1 8088 758 12 7945 3.9 1 7558 387 13 6935 1.0 1 6642 293 14 7727 1.0 1 7181 546 15 6991 1.0 1 6693 298 16 7577 1.0 1 6962 615 17 7190 1.0 1 6725 465 18 6354 1.0 1 6115 239 19 6233 1.0 1 5893 340 20 6134 1.0 1 5893 241 21 6128 1.0 1 5817 311 22 5568 1.0 1 5361 207 23 5681 1.0 1 5410 271 24 6117 1.0 1 5633 484 25 5216 1.0 1 4988 228 26 5180 1.0 1 4931 249 27 5224 1.0 1 4988 236 28 4454 1.0 1 4285 169 29 5204 1.0 1 4847 357 30 4488 1.0 1 4335 153 31 4275 1.0 1 4083 192 32 4134 1.0 1 3986 148 33 4425 1.0 1 4135 290 34 4297 1.0 1 4085 212 35 3892 1.0 1 3712 180 36 3567 1.0 1 3454 113 37 3854 1.0 1 3641 213 38 3952 1.0 1 3702 250 39 3553 1.0 1 3412 141 40 3330 1.0 1 3195 135 41 3298 1.0 1 3178 120 42 3553 1.0 1 3387 166 43 3757 1.0 1 3411 346 44 3629 1.0 1 3389 240 45 4413 1.0 1 4245 168 46 1991 1.0 1 1862 129 47 2458 1.0 1 2371 87 48 4364 1.0 1 4191 173 49 2811 1.0 1 2702 109 50 2998 1.0 1 2872 126 51 5573 1.0 1 5355 218 52 1675 1.0 1 1589 86 53 1166 1.0 1 1122 44 54 3122 1.0 1 3022 100 55 3358 1.0 1 3273 85 56 2952 1.0 1 2863 89 57 2031 1.0 1 1988 43 58 1293 1.0 1 1227 66 59 3891 1.0 1 3770 121 60 723 1.0 1 691 32 61 1179 1.0 1 1119 60 62 2436 1.0 1 2372 64 63 1603 1.0 1 1566 37 64 832 1.0 1 797 35 65 2109 1.0 1 2046 63 66 1959 1.0 1 1910 49 67 1144 1.0 1 1095 49 68 2241 1.0 1 2165 76 69 2457 1.0 1 2362 95 70 4897 1.0 1 4735 162 71 574 1.0 1 532 42 72 165 1.0 1 147 18 73 487 1.0 1 471 16 74 1090 1.0 1 1053 37 75 1413 1.0 1 1383 30 76 1623 1.0 1 1582 41 77 1671 1.0 1 1606 65 78 1655 1.0 1 1611 44 79 1704 1.0 1 1632 72 80 1645 1.0 1 1591 54 81 1675 1.0 1 1608 67 82 1566 1.0 1 1523 43 83 1620 1.0 1 1565 55 84 1588 1.0 1 1537 51 85 1611 1.0 1 1559 52 86 1566 1.0 1 1515 51 87 1565 1.0 1 1520 45 88 1424 1.0 1 1381 43 89 1596 1.0 1 1552 44 90 1532 1.0 1 1488 44 91 1497 1.0 1 1449 48 92 1446 1.0 1 1394 52 93 1464 1.0 1 1431 33 94 1452 1.0 1 1412 40 95 1503 1.0 1 1470 33 96 1368 1.0 1 1337 31 97 1334 1.0 1 1293 41 98 1414 1.0 1 1374 40 99 1441 1.0 1 1408 33 100 1402 1.0 1 1362 40 101 1380 1.0 1 1343 37 102 1396 1.0 1 1357 39 103 1287 1.0 1 1255 32 104 1280 1.0 1 1255 25 105 1242 1.0 1 1208 34 106 1303 1.0 1 1274 29 107 1274 1.0 1 1241 33 108 1173 1.0 1 1154 19 109 1146 1.0 1 1119 27 110 1221 1.0 1 1191 30 111 1239 1.0 1 1214 25 112 1110 1.0 1 1092 18 113 1177 1.0 1 1143 34 114 1064 1.0 1 1038 26 115 1036 1.0 1 1010 26 116 1075 1.0 1 1037 38 117 1116 1.0 1 1090 26 118 1018 1.0 1 1000 18 119 1101 1.0 1 1076 25 120 1235 1.0 1 1204 31 121 1171 1.0 1 1147 24 122 1161 1.0 1 1142 19 123 1175 1.0 1 1149 26 124 1134 1.0 1 1122 12 125 1072 1.0 1 1050 22 126 1013 1.0 1 1000 13 127 919 1.0 1 904 15 128 769 1.0 1 754 15 129 718 1.0 1 707 11 130 623 1.0 1 607 16 131 584 1.0 1 578 6 132 687 1.0 1 676 11 133 624 1.0 1 616 8 134 618 1.0 1 608 10 135 572 1.0 1 561 11 136 582 1.0 1 571 11 137 682 1.0 1 670 12 138 799 1.0 1 782 17 139 987 1.0 1 969 18 140 1294 1.0 1 1268 26 141 2097 1.0 1 2059 38 142 2403 1.0 1 2371 32 143 3270 1.0 1 3232 38 144 4628 1.0 1 4577 51 145 8391 1.0 1 8297 94 146 5265 1.0 1 5214 51 147 1370 1.0 1 1340 30 148 23008 1.0 1 22738 270 149 8125 1.0 1 7961 164 150 83666 1.0 1 81953 1713 RUN STATISTICS FOR INPUT FILE: SRR18245605_1.fastq.gz ============================================= 65019432 sequences processed in total