SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245602_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 52392). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245602_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4222.425 s (53.202 µs/read; 1.13 M reads/minute). === Summary === Total reads processed: 79,365,519 Reads with adapters: 32,642,423 (41.1%) Reads written (passing filters): 79,365,519 (100.0%) Total basepairs processed: 11,904,827,850 bp Quality-trimmed: 15,411,809 bp (0.1%) Total written (filtered): 11,647,961,590 bp (97.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 32642423 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 28.6% C: 16.8% G: 12.0% T: 42.3% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 21523447 19841379.8 0 21523447 2 3423271 4960344.9 0 3423271 3 1388328 1240086.2 0 1388328 4 742752 310021.6 0 742752 5 181430 77505.4 0 181430 6 160831 19376.3 0 160831 7 158986 4844.1 0 158986 8 165361 1211.0 0 165361 9 163174 302.8 0 162497 677 10 147086 75.7 1 141386 5700 11 162852 18.9 1 152350 10502 12 153677 4.7 1 146885 6792 13 126007 1.2 1 120979 5028 14 144069 1.2 1 134664 9405 15 125922 1.2 1 120643 5279 16 137270 1.2 1 127528 9742 17 126887 1.2 1 119301 7586 18 113548 1.2 1 108893 4655 19 111724 1.2 1 106566 5158 20 108193 1.2 1 103114 5079 21 107647 1.2 1 102082 5565 22 98462 1.2 1 94682 3780 23 99246 1.2 1 94388 4858 24 102903 1.2 1 95895 7008 25 86952 1.2 1 83117 3835 26 90050 1.2 1 85758 4292 27 85268 1.2 1 81641 3627 28 75370 1.2 1 72578 2792 29 83301 1.2 1 78159 5142 30 72450 1.2 1 69812 2638 31 70463 1.2 1 67491 2972 32 73486 1.2 1 70651 2835 33 61977 1.2 1 58766 3211 34 65028 1.2 1 62028 3000 35 61497 1.2 1 58818 2679 36 61761 1.2 1 59005 2756 37 54326 1.2 1 52078 2248 38 53306 1.2 1 51436 1870 39 50976 1.2 1 49175 1801 40 52088 1.2 1 49396 2692 41 48127 1.2 1 46429 1698 42 57143 1.2 1 54475 2668 43 44092 1.2 1 40789 3303 44 51231 1.2 1 48043 3188 45 77144 1.2 1 74281 2863 46 26777 1.2 1 24998 1779 47 39213 1.2 1 37184 2029 48 185885 1.2 1 182000 3885 49 43427 1.2 1 41800 1627 50 20657 1.2 1 19373 1284 51 67793 1.2 1 65767 2026 52 28243 1.2 1 27309 934 53 8209 1.2 1 7799 410 54 41950 1.2 1 41036 914 55 36993 1.2 1 36486 507 56 17166 1.2 1 16681 485 57 13466 1.2 1 13095 371 58 7045 1.2 1 6736 309 59 29669 1.2 1 28966 703 60 4160 1.2 1 3960 200 61 6658 1.2 1 6359 299 62 19164 1.2 1 18821 343 63 8022 1.2 1 7828 194 64 3898 1.2 1 3647 251 65 14550 1.2 1 14133 417 66 18747 1.2 1 18418 329 67 4575 1.2 1 4331 244 68 13156 1.2 1 12720 436 69 16412 1.2 1 15833 579 70 25846 1.2 1 25072 774 71 4734 1.2 1 4398 336 72 1449 1.2 1 1310 139 73 3076 1.2 1 2959 117 74 8321 1.2 1 8136 185 75 12719 1.2 1 12404 315 76 14385 1.2 1 14022 363 77 14807 1.2 1 14448 359 78 15178 1.2 1 14723 455 79 14816 1.2 1 14420 396 80 14942 1.2 1 14531 411 81 14545 1.2 1 14112 433 82 14451 1.2 1 14033 418 83 14094 1.2 1 13691 403 84 13707 1.2 1 13323 384 85 13683 1.2 1 13303 380 86 12939 1.2 1 12594 345 87 13095 1.2 1 12720 375 88 12651 1.2 1 12255 396 89 12491 1.2 1 12122 369 90 12608 1.2 1 12243 365 91 12037 1.2 1 11701 336 92 11964 1.2 1 11605 359 93 11824 1.2 1 11479 345 94 11680 1.2 1 11331 349 95 11416 1.2 1 11085 331 96 11219 1.2 1 10896 323 97 10923 1.2 1 10619 304 98 11012 1.2 1 10696 316 99 10604 1.2 1 10323 281 100 10737 1.2 1 10411 326 101 10626 1.2 1 10336 290 102 10476 1.2 1 10173 303 103 10326 1.2 1 10066 260 104 10287 1.2 1 10006 281 105 10108 1.2 1 9821 287 106 9986 1.2 1 9705 281 107 9747 1.2 1 9465 282 108 9855 1.2 1 9604 251 109 9580 1.2 1 9342 238 110 9548 1.2 1 9280 268 111 9643 1.2 1 9389 254 112 9449 1.2 1 9215 234 113 9140 1.2 1 8893 247 114 9387 1.2 1 9152 235 115 9335 1.2 1 9108 227 116 9326 1.2 1 9112 214 117 9538 1.2 1 9291 247 118 9839 1.2 1 9635 204 119 10440 1.2 1 10216 224 120 11209 1.2 1 10994 215 121 11750 1.2 1 11523 227 122 11805 1.2 1 11565 240 123 11422 1.2 1 11211 211 124 11087 1.2 1 10908 179 125 9888 1.2 1 9623 265 126 8821 1.2 1 8664 157 127 7942 1.2 1 7810 132 128 7282 1.2 1 7171 111 129 6811 1.2 1 6715 96 130 6073 1.2 1 5987 86 131 5761 1.2 1 5683 78 132 5378 1.2 1 5290 88 133 5302 1.2 1 5220 82 134 4724 1.2 1 4615 109 135 4032 1.2 1 3929 103 136 3566 1.2 1 3467 99 137 3353 1.2 1 3287 66 138 3799 1.2 1 3727 72 139 3475 1.2 1 3392 83 140 3367 1.2 1 3308 59 141 4232 1.2 1 4154 78 142 3431 1.2 1 3362 69 143 3581 1.2 1 3485 96 144 4653 1.2 1 4449 204 145 5909 1.2 1 5546 363 146 3326 1.2 1 3058 268 147 446 1.2 1 363 83 148 5895 1.2 1 3606 2289 149 2108 1.2 1 1736 372 150 72462 1.2 1 61252 11210 RUN STATISTICS FOR INPUT FILE: SRR18245602_1.fastq.gz ============================================= 79365519 sequences processed in total