Bismark report for: SRR18245598_val_1.fq.gz and SRR18245598_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 91557616 Number of paired-end alignments with a unique best hit: 52522242 Mapping efficiency: 57.4% Sequence pairs with no alignments under any condition: 26450329 Sequence pairs did not map uniquely: 12585045 Sequence pairs which were discarded because genomic sequence could not be extracted: 19 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 26262746 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 26259477 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2439445332 Total methylated C's in CpG context: 37489864 Total methylated C's in CHG context: 11716116 Total methylated C's in CHH context: 49505624 Total methylated C's in Unknown context: 1641170 Total unmethylated C's in CpG context: 298625553 Total unmethylated C's in CHG context: 425858776 Total unmethylated C's in CHH context: 1616249399 Total unmethylated C's in Unknown context: 9770178 C methylated in CpG context: 11.2% C methylated in CHG context: 2.7% C methylated in CHH context: 3.0% C methylated in Unknown context (CN or CHN): 14.4% Bismark completed in 0d 11h 59m 20s