SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245598_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 234452). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245598_1.fastq.gz Processing single-end reads on 1 core ... Finished in 5184.304 s (53.602 µs/read; 1.12 M reads/minute). === Summary === Total reads processed: 96,717,703 Reads with adapters: 52,341,678 (54.1%) Reads written (passing filters): 96,717,703 (100.0%) Total basepairs processed: 14,507,655,450 bp Quality-trimmed: 15,211,602 bp (0.1%) Total written (filtered): 12,526,067,223 bp (86.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 52341678 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 17.5% C: 41.2% G: 7.0% T: 29.7% none/other: 4.6% Overview of removed sequences length count expect max.err error counts 1 20411174 24179425.8 0 20411174 2 3477166 6044856.4 0 3477166 3 1518755 1511214.1 0 1518755 4 851369 377803.5 0 851369 5 361707 94450.9 0 361707 6 321160 23612.7 0 321160 7 312949 5903.2 0 312949 8 336193 1475.8 0 336193 9 313991 368.9 0 313128 863 10 304026 92.2 1 293219 10807 11 334478 23.1 1 312589 21889 12 308294 5.8 1 294887 13407 13 292011 1.4 1 280532 11479 14 303996 1.4 1 286232 17764 15 289967 1.4 1 276698 13269 16 293995 1.4 1 275681 18314 17 301667 1.4 1 282914 18753 18 282884 1.4 1 271915 10969 19 238876 1.4 1 228895 9981 20 266936 1.4 1 254444 12492 21 256156 1.4 1 244279 11877 22 243067 1.4 1 234164 8903 23 255636 1.4 1 243335 12301 24 246762 1.4 1 231642 15120 25 239977 1.4 1 229494 10483 26 224646 1.4 1 214981 9665 27 229709 1.4 1 219026 10683 28 223426 1.4 1 214413 9013 29 222115 1.4 1 209415 12700 30 211416 1.4 1 203742 7674 31 194762 1.4 1 187314 7448 32 202150 1.4 1 194560 7590 33 200799 1.4 1 191008 9791 34 203175 1.4 1 193431 9744 35 178274 1.4 1 173273 5001 36 202135 1.4 1 193660 8475 37 191478 1.4 1 179305 12173 38 171862 1.4 1 164309 7553 39 172850 1.4 1 164875 7975 40 172461 1.4 1 165714 6747 41 172005 1.4 1 166553 5452 42 160058 1.4 1 154930 5128 43 165537 1.4 1 156639 8898 44 163478 1.4 1 154986 8492 45 226346 1.4 1 219705 6641 46 81513 1.4 1 76879 4634 47 133347 1.4 1 129538 3809 48 159303 1.4 1 153992 5311 49 128830 1.4 1 124634 4196 50 149920 1.4 1 143252 6668 51 155568 1.4 1 147753 7815 52 134725 1.4 1 130370 4355 53 128497 1.4 1 124824 3673 54 141878 1.4 1 136308 5570 55 148201 1.4 1 143717 4484 56 120742 1.4 1 116194 4548 57 154301 1.4 1 149829 4472 58 129039 1.4 1 124744 4295 59 121238 1.4 1 116344 4894 60 133583 1.4 1 130689 2894 61 92779 1.4 1 89280 3499 62 129859 1.4 1 125964 3895 63 153060 1.4 1 149834 3226 64 104194 1.4 1 101211 2983 65 94704 1.4 1 91299 3405 66 125695 1.4 1 122574 3121 67 133443 1.4 1 129489 3954 68 173365 1.4 1 167965 5400 69 161475 1.4 1 155145 6330 70 321076 1.4 1 310921 10155 71 18115 1.4 1 16266 1849 72 8885 1.4 1 7987 898 73 43500 1.4 1 42422 1078 74 83353 1.4 1 81564 1789 75 101906 1.4 1 99469 2437 76 108182 1.4 1 105448 2734 77 111423 1.4 1 108573 2850 78 112140 1.4 1 109101 3039 79 113087 1.4 1 110055 3032 80 113412 1.4 1 110392 3020 81 114138 1.4 1 111013 3125 82 114225 1.4 1 111171 3054 83 114349 1.4 1 111098 3251 84 115251 1.4 1 112118 3133 85 116158 1.4 1 113020 3138 86 115174 1.4 1 112068 3106 87 115178 1.4 1 111994 3184 88 115021 1.4 1 111917 3104 89 116729 1.4 1 113640 3089 90 116033 1.4 1 112891 3142 91 117626 1.4 1 114407 3219 92 117872 1.4 1 114718 3154 93 118816 1.4 1 115773 3043 94 119847 1.4 1 116835 3012 95 121325 1.4 1 118199 3126 96 122288 1.4 1 119228 3060 97 123281 1.4 1 120250 3031 98 123722 1.4 1 120718 3004 99 124521 1.4 1 121493 3028 100 126088 1.4 1 123124 2964 101 127256 1.4 1 124269 2987 102 128748 1.4 1 125660 3088 103 129074 1.4 1 126017 3057 104 130250 1.4 1 127242 3008 105 131598 1.4 1 128559 3039 106 132470 1.4 1 129479 2991 107 133816 1.4 1 130897 2919 108 134362 1.4 1 131426 2936 109 135717 1.4 1 132826 2891 110 137217 1.4 1 134336 2881 111 138470 1.4 1 135494 2976 112 140062 1.4 1 137048 3014 113 143069 1.4 1 139918 3151 114 146425 1.4 1 143399 3026 115 150484 1.4 1 147450 3034 116 156526 1.4 1 153469 3057 117 164773 1.4 1 161481 3292 118 178296 1.4 1 174969 3327 119 200470 1.4 1 196788 3682 120 225204 1.4 1 221468 3736 121 243908 1.4 1 239975 3933 122 247130 1.4 1 243179 3951 123 250629 1.4 1 246802 3827 124 242532 1.4 1 238842 3690 125 217646 1.4 1 212019 5627 126 191744 1.4 1 188714 3030 127 175345 1.4 1 172744 2601 128 162634 1.4 1 160339 2295 129 147029 1.4 1 144886 2143 130 136231 1.4 1 134262 1969 131 135292 1.4 1 133360 1932 132 128616 1.4 1 126506 2110 133 129272 1.4 1 126870 2402 134 116179 1.4 1 113570 2609 135 102604 1.4 1 99756 2848 136 90710 1.4 1 88265 2445 137 87305 1.4 1 85084 2221 138 97006 1.4 1 94063 2943 139 83679 1.4 1 80649 3030 140 83970 1.4 1 80658 3312 141 102719 1.4 1 98231 4488 142 84079 1.4 1 80264 3815 143 90594 1.4 1 85481 5113 144 119794 1.4 1 110418 9376 145 148533 1.4 1 132585 15948 146 80858 1.4 1 70731 10127 147 9923 1.4 1 7395 2528 148 235707 1.4 1 145381 90326 149 74636 1.4 1 60701 13935 150 2417233 1.4 1 2053614 363619 RUN STATISTICS FOR INPUT FILE: SRR18245598_1.fastq.gz ============================================= 96717703 sequences processed in total