SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245597_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 248121). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245597_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4888.993 s (51.972 µs/read; 1.15 M reads/minute). === Summary === Total reads processed: 94,070,392 Reads with adapters: 51,548,148 (54.8%) Reads written (passing filters): 94,070,392 (100.0%) Total basepairs processed: 14,110,558,800 bp Quality-trimmed: 14,671,334 bp (0.1%) Total written (filtered): 12,112,088,717 bp (85.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 51548148 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 16.8% C: 42.6% G: 7.1% T: 28.9% none/other: 4.7% Overview of removed sequences length count expect max.err error counts 1 19324524 23517598.0 0 19324524 2 3392058 5879399.5 0 3392058 3 1467904 1469849.9 0 1467904 4 815059 367462.5 0 815059 5 344003 91865.6 0 344003 6 304055 22966.4 0 304055 7 297621 5741.6 0 297621 8 318477 1435.4 0 318477 9 301788 358.9 0 300937 851 10 291992 89.7 1 281779 10213 11 321598 22.4 1 300837 20761 12 296983 5.6 1 284380 12603 13 282311 1.4 1 271329 10982 14 296566 1.4 1 279427 17139 15 282147 1.4 1 269471 12676 16 288183 1.4 1 269924 18259 17 295066 1.4 1 277074 17992 18 274083 1.4 1 263767 10316 19 242842 1.4 1 232614 10228 20 266418 1.4 1 254153 12265 21 257419 1.4 1 245609 11810 22 243081 1.4 1 234394 8687 23 254015 1.4 1 241981 12034 24 254332 1.4 1 238862 15470 25 243095 1.4 1 232654 10441 26 230529 1.4 1 220476 10053 27 234553 1.4 1 223843 10710 28 223923 1.4 1 215467 8456 29 237396 1.4 1 223445 13951 30 219158 1.4 1 211306 7852 31 201690 1.4 1 193747 7943 32 210670 1.4 1 202706 7964 33 210407 1.4 1 199921 10486 34 209475 1.4 1 199705 9770 35 212742 1.4 1 203476 9266 36 187640 1.4 1 180329 7311 37 182290 1.4 1 176921 5369 38 202981 1.4 1 194265 8716 39 193006 1.4 1 180402 12604 40 179887 1.4 1 173508 6379 41 185348 1.4 1 178698 6650 42 208841 1.4 1 202164 6677 43 151967 1.4 1 142568 9399 44 174123 1.4 1 165549 8574 45 252209 1.4 1 244433 7776 46 86014 1.4 1 81057 4957 47 151529 1.4 1 146889 4640 48 174300 1.4 1 168291 6009 49 142538 1.4 1 137968 4570 50 164308 1.4 1 156652 7656 51 168339 1.4 1 160133 8206 52 151031 1.4 1 146178 4853 53 144595 1.4 1 140266 4329 54 157133 1.4 1 150872 6261 55 162271 1.4 1 157309 4962 56 132535 1.4 1 127886 4649 57 165881 1.4 1 160647 5234 58 151500 1.4 1 146542 4958 59 129526 1.4 1 124502 5024 60 149597 1.4 1 146102 3495 61 111406 1.4 1 107423 3983 62 142767 1.4 1 138063 4704 63 164138 1.4 1 160174 3964 64 126881 1.4 1 123276 3605 65 105068 1.4 1 101416 3652 66 135933 1.4 1 132435 3498 67 150442 1.4 1 145764 4678 68 191228 1.4 1 185239 5989 69 177711 1.4 1 170567 7144 70 368205 1.4 1 356326 11879 71 19387 1.4 1 17265 2122 72 9180 1.4 1 8181 999 73 47851 1.4 1 46656 1195 74 92612 1.4 1 90600 2012 75 111968 1.4 1 109319 2649 76 118879 1.4 1 115907 2972 77 121533 1.4 1 118302 3231 78 122687 1.4 1 119340 3347 79 122985 1.4 1 119542 3443 80 123234 1.4 1 119706 3528 81 122845 1.4 1 119299 3546 82 123774 1.4 1 120280 3494 83 123747 1.4 1 120288 3459 84 123389 1.4 1 120072 3317 85 123312 1.4 1 119823 3489 86 123999 1.4 1 120443 3556 87 123223 1.4 1 119879 3344 88 123119 1.4 1 119649 3470 89 124276 1.4 1 120825 3451 90 123551 1.4 1 120133 3418 91 124103 1.4 1 120818 3285 92 124002 1.4 1 120628 3374 93 124284 1.4 1 121026 3258 94 124707 1.4 1 121484 3223 95 126252 1.4 1 122886 3366 96 125935 1.4 1 122750 3185 97 126357 1.4 1 123131 3226 98 126561 1.4 1 123412 3149 99 127477 1.4 1 124390 3087 100 128260 1.4 1 125114 3146 101 129251 1.4 1 126217 3034 102 130392 1.4 1 127291 3101 103 130781 1.4 1 127767 3014 104 131746 1.4 1 128766 2980 105 132063 1.4 1 129001 3062 106 134063 1.4 1 131032 3031 107 133124 1.4 1 130083 3041 108 134436 1.4 1 131364 3072 109 134838 1.4 1 131913 2925 110 135200 1.4 1 132258 2942 111 136363 1.4 1 133419 2944 112 138798 1.4 1 135868 2930 113 140586 1.4 1 137468 3118 114 143044 1.4 1 139956 3088 115 146214 1.4 1 143074 3140 116 152581 1.4 1 149498 3083 117 162656 1.4 1 159489 3167 118 176789 1.4 1 173524 3265 119 201682 1.4 1 198130 3552 120 229537 1.4 1 225421 4116 121 251098 1.4 1 246873 4225 122 255992 1.4 1 251857 4135 123 261858 1.4 1 257712 4146 124 251359 1.4 1 247572 3787 125 222948 1.4 1 216796 6152 126 191505 1.4 1 188333 3172 127 171370 1.4 1 168774 2596 128 157223 1.4 1 154946 2277 129 137383 1.4 1 135121 2262 130 123897 1.4 1 122009 1888 131 121845 1.4 1 120003 1842 132 115437 1.4 1 113462 1975 133 116001 1.4 1 113635 2366 134 102674 1.4 1 100118 2556 135 90865 1.4 1 88157 2708 136 78935 1.4 1 76585 2350 137 75863 1.4 1 73846 2017 138 84437 1.4 1 81905 2532 139 72458 1.4 1 69988 2470 140 72835 1.4 1 70141 2694 141 91234 1.4 1 87505 3729 142 73715 1.4 1 70400 3315 143 83088 1.4 1 78551 4537 144 105243 1.4 1 96890 8353 145 132238 1.4 1 116803 15435 146 71588 1.4 1 62150 9438 147 9471 1.4 1 7038 2433 148 242392 1.4 1 148863 93529 149 74228 1.4 1 60026 14202 150 2402004 1.4 1 2036927 365077 RUN STATISTICS FOR INPUT FILE: SRR18245597_1.fastq.gz ============================================= 94070392 sequences processed in total