Bismark report for: SRR18245596_val_1.fq.gz and SRR18245596_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 83281726 Number of paired-end alignments with a unique best hit: 48870727 Mapping efficiency: 58.7% Sequence pairs with no alignments under any condition: 26385245 Sequence pairs did not map uniquely: 8025754 Sequence pairs which were discarded because genomic sequence could not be extracted: 20 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 24409397 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 24461310 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2397123341 Total methylated C's in CpG context: 35335481 Total methylated C's in CHG context: 8980274 Total methylated C's in CHH context: 37987892 Total methylated C's in Unknown context: 1213009 Total unmethylated C's in CpG context: 273577676 Total unmethylated C's in CHG context: 407121134 Total unmethylated C's in CHH context: 1634120884 Total unmethylated C's in Unknown context: 8913543 C methylated in CpG context: 11.4% C methylated in CHG context: 2.2% C methylated in CHH context: 2.3% C methylated in Unknown context (CN or CHN): 12.0% Bismark completed in 0d 11h 5m 25s