SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245596_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 8007). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245596_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4425.653 s (53.121 µs/read; 1.13 M reads/minute). === Summary === Total reads processed: 83,312,485 Reads with adapters: 32,300,932 (38.8%) Reads written (passing filters): 83,312,485 (100.0%) Total basepairs processed: 12,496,872,750 bp Quality-trimmed: 13,688,044 bp (0.1%) Total written (filtered): 12,409,431,842 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 32300932 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.3% C: 2.9% G: 13.6% T: 47.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 25128783 20828121.2 0 25128783 2 4080649 5207030.3 0 4080649 3 1517348 1301757.6 0 1517348 4 649166 325439.4 0 649166 5 34191 81359.8 0 34191 6 30872 20340.0 0 30872 7 28268 5085.0 0 28268 8 28359 1271.2 0 28359 9 27394 317.8 0 26647 747 10 26584 79.5 1 24359 2225 11 27449 19.9 1 25114 2335 12 25227 5.0 1 23813 1414 13 21834 1.2 1 20831 1003 14 23279 1.2 1 21504 1775 15 21180 1.2 1 20089 1091 16 21592 1.2 1 19811 1781 17 20919 1.2 1 19453 1466 18 18951 1.2 1 18023 928 19 17188 1.2 1 16270 918 20 17269 1.2 1 16338 931 21 16913 1.2 1 15954 959 22 15632 1.2 1 14911 721 23 15839 1.2 1 14916 923 24 15722 1.2 1 14487 1235 25 14067 1.2 1 13312 755 26 13639 1.2 1 12950 689 27 13608 1.2 1 12889 719 28 12729 1.2 1 12176 553 29 12506 1.2 1 11616 890 30 12049 1.2 1 11460 589 31 10794 1.2 1 10229 565 32 11263 1.2 1 10805 458 33 10498 1.2 1 9902 596 34 10840 1.2 1 10301 539 35 11681 1.2 1 11248 433 36 7011 1.2 1 6579 432 37 10182 1.2 1 9402 780 38 9143 1.2 1 8608 535 39 8649 1.2 1 8265 384 40 7996 1.2 1 7603 393 41 7746 1.2 1 7428 318 42 8150 1.2 1 7781 369 43 8256 1.2 1 7687 569 44 6927 1.2 1 6543 384 45 10546 1.2 1 10164 382 46 3184 1.2 1 2981 203 47 5913 1.2 1 5733 180 48 7000 1.2 1 6696 304 49 5706 1.2 1 5443 263 50 6203 1.2 1 5888 315 51 6509 1.2 1 6109 400 52 5720 1.2 1 5451 269 53 5025 1.2 1 4852 173 54 5492 1.2 1 5258 234 55 5738 1.2 1 5493 245 56 4698 1.2 1 4469 229 57 5743 1.2 1 5547 196 58 4733 1.2 1 4572 161 59 4643 1.2 1 4431 212 60 4541 1.2 1 4435 106 61 3194 1.2 1 3057 137 62 4577 1.2 1 4393 184 63 5052 1.2 1 4944 108 64 3350 1.2 1 3223 127 65 3101 1.2 1 2953 148 66 4606 1.2 1 4450 156 67 4235 1.2 1 4101 134 68 5824 1.2 1 5636 188 69 4819 1.2 1 4609 210 70 7955 1.2 1 7640 315 71 528 1.2 1 467 61 72 370 1.2 1 333 37 73 1480 1.2 1 1421 59 74 2532 1.2 1 2454 78 75 2751 1.2 1 2679 72 76 2915 1.2 1 2818 97 77 3019 1.2 1 2922 97 78 2969 1.2 1 2875 94 79 2832 1.2 1 2733 99 80 2801 1.2 1 2710 91 81 2909 1.2 1 2792 117 82 2699 1.2 1 2608 91 83 2817 1.2 1 2725 92 84 2654 1.2 1 2553 101 85 2476 1.2 1 2390 86 86 2443 1.2 1 2373 70 87 2447 1.2 1 2348 99 88 2556 1.2 1 2486 70 89 2506 1.2 1 2433 73 90 2408 1.2 1 2328 80 91 2394 1.2 1 2323 71 92 2371 1.2 1 2282 89 93 2263 1.2 1 2196 67 94 2168 1.2 1 2085 83 95 2183 1.2 1 2120 63 96 2177 1.2 1 2103 74 97 2123 1.2 1 2041 82 98 2019 1.2 1 1935 84 99 2028 1.2 1 1955 73 100 2000 1.2 1 1930 70 101 1937 1.2 1 1880 57 102 1904 1.2 1 1847 57 103 1865 1.2 1 1817 48 104 1806 1.2 1 1749 57 105 1802 1.2 1 1742 60 106 1794 1.2 1 1740 54 107 1681 1.2 1 1613 68 108 1713 1.2 1 1656 57 109 1708 1.2 1 1648 60 110 1570 1.2 1 1525 45 111 1533 1.2 1 1484 49 112 1562 1.2 1 1512 50 113 1507 1.2 1 1469 38 114 1432 1.2 1 1385 47 115 1546 1.2 1 1494 52 116 1418 1.2 1 1376 42 117 1370 1.2 1 1333 37 118 1369 1.2 1 1327 42 119 1400 1.2 1 1363 37 120 1315 1.2 1 1278 37 121 1291 1.2 1 1256 35 122 1214 1.2 1 1188 26 123 1179 1.2 1 1152 27 124 1094 1.2 1 1064 30 125 967 1.2 1 940 27 126 895 1.2 1 874 21 127 830 1.2 1 810 20 128 765 1.2 1 750 15 129 729 1.2 1 712 17 130 622 1.2 1 610 12 131 580 1.2 1 566 14 132 507 1.2 1 490 17 133 441 1.2 1 424 17 134 356 1.2 1 342 14 135 298 1.2 1 282 16 136 257 1.2 1 240 17 137 245 1.2 1 233 12 138 218 1.2 1 203 15 139 245 1.2 1 230 15 140 217 1.2 1 209 8 141 315 1.2 1 300 15 142 250 1.2 1 240 10 143 263 1.2 1 244 19 144 352 1.2 1 324 28 145 468 1.2 1 413 55 146 219 1.2 1 201 18 147 72 1.2 1 61 11 148 482 1.2 1 376 106 149 599 1.2 1 506 93 150 6443 1.2 1 5414 1029 RUN STATISTICS FOR INPUT FILE: SRR18245596_1.fastq.gz ============================================= 83312485 sequences processed in total