SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245595_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 45998). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245595_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4603.931 s (56.930 µs/read; 1.05 M reads/minute). === Summary === Total reads processed: 80,869,598 Reads with adapters: 33,295,312 (41.2%) Reads written (passing filters): 80,869,598 (100.0%) Total basepairs processed: 12,130,439,700 bp Quality-trimmed: 10,622,025 bp (0.1%) Total written (filtered): 11,910,451,785 bp (98.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 33295312 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 29.7% C: 15.4% G: 12.6% T: 42.1% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 22103439 20217399.5 0 22103439 2 3777159 5054349.9 0 3777159 3 1493478 1263587.5 0 1493478 4 756016 315896.9 0 756016 5 191928 78974.2 0 191928 6 172787 19743.6 0 172787 7 166426 4935.9 0 166426 8 174463 1234.0 0 174463 9 156018 308.5 0 155324 694 10 154814 77.1 1 148558 6256 11 162453 19.3 1 152010 10443 12 153685 4.8 1 146465 7220 13 135516 1.2 1 130145 5371 14 140709 1.2 1 132112 8597 15 132787 1.2 1 126683 6104 16 131477 1.2 1 122644 8833 17 127916 1.2 1 120446 7470 18 122840 1.2 1 117548 5292 19 103805 1.2 1 99119 4686 20 109745 1.2 1 104684 5061 21 105568 1.2 1 100423 5145 22 98087 1.2 1 94052 4035 23 98773 1.2 1 94019 4754 24 98020 1.2 1 91738 6282 25 87958 1.2 1 84089 3869 26 86333 1.2 1 82428 3905 27 84664 1.2 1 80955 3709 28 75438 1.2 1 72704 2734 29 77748 1.2 1 73226 4522 30 72628 1.2 1 69844 2784 31 67737 1.2 1 65056 2681 32 65409 1.2 1 62986 2423 33 65375 1.2 1 61771 3604 34 64064 1.2 1 60878 3186 35 57212 1.2 1 54516 2696 36 55315 1.2 1 53287 2028 37 51810 1.2 1 49940 1870 38 53634 1.2 1 51303 2331 39 58254 1.2 1 56340 1914 40 36094 1.2 1 33851 2243 41 44657 1.2 1 42826 1831 42 41913 1.2 1 40529 1384 43 44604 1.2 1 42217 2387 44 40358 1.2 1 38130 2228 45 55348 1.2 1 53589 1759 46 18842 1.2 1 17738 1104 47 31135 1.2 1 30214 921 48 38111 1.2 1 36716 1395 49 28833 1.2 1 27783 1050 50 31617 1.2 1 30143 1474 51 34405 1.2 1 32610 1795 52 27368 1.2 1 26436 932 53 22696 1.2 1 22118 578 54 28351 1.2 1 27420 931 55 29647 1.2 1 28760 887 56 21845 1.2 1 20983 862 57 29094 1.2 1 28430 664 58 18142 1.2 1 17554 588 59 23460 1.2 1 22591 869 60 18734 1.2 1 18356 378 61 11540 1.2 1 11094 446 62 21595 1.2 1 20940 655 63 24544 1.2 1 24190 354 64 10804 1.2 1 10446 358 65 13165 1.2 1 12680 485 66 19508 1.2 1 19015 493 67 16235 1.2 1 15797 438 68 21715 1.2 1 21129 586 69 19671 1.2 1 18949 722 70 31257 1.2 1 30331 926 71 2497 1.2 1 2261 236 72 1446 1.2 1 1347 99 73 5287 1.2 1 5129 158 74 9495 1.2 1 9249 246 75 11117 1.2 1 10834 283 76 11309 1.2 1 10953 356 77 11092 1.2 1 10745 347 78 11211 1.2 1 10876 335 79 10972 1.2 1 10637 335 80 10672 1.2 1 10344 328 81 10274 1.2 1 9938 336 82 10166 1.2 1 9836 330 83 9936 1.2 1 9593 343 84 9558 1.2 1 9273 285 85 9164 1.2 1 8889 275 86 9237 1.2 1 8933 304 87 9086 1.2 1 8830 256 88 8742 1.2 1 8483 259 89 8442 1.2 1 8187 255 90 8349 1.2 1 8083 266 91 8036 1.2 1 7805 231 92 7922 1.2 1 7692 230 93 7743 1.2 1 7535 208 94 7672 1.2 1 7455 217 95 7424 1.2 1 7207 217 96 7460 1.2 1 7228 232 97 7405 1.2 1 7146 259 98 6995 1.2 1 6792 203 99 6833 1.2 1 6630 203 100 6924 1.2 1 6743 181 101 6654 1.2 1 6466 188 102 6651 1.2 1 6463 188 103 6523 1.2 1 6351 172 104 6419 1.2 1 6227 192 105 6337 1.2 1 6127 210 106 6309 1.2 1 6128 181 107 6275 1.2 1 6097 178 108 6043 1.2 1 5875 168 109 6223 1.2 1 6073 150 110 5956 1.2 1 5783 173 111 5955 1.2 1 5793 162 112 5861 1.2 1 5708 153 113 5905 1.2 1 5758 147 114 6021 1.2 1 5884 137 115 5854 1.2 1 5721 133 116 5887 1.2 1 5741 146 117 6195 1.2 1 6074 121 118 6716 1.2 1 6560 156 119 7541 1.2 1 7399 142 120 8215 1.2 1 8067 148 121 8809 1.2 1 8643 166 122 9149 1.2 1 8987 162 123 8816 1.2 1 8655 161 124 8318 1.2 1 8179 139 125 7353 1.2 1 7126 227 126 5995 1.2 1 5887 108 127 5359 1.2 1 5266 93 128 4798 1.2 1 4715 83 129 4225 1.2 1 4141 84 130 3757 1.2 1 3690 67 131 3527 1.2 1 3467 60 132 3196 1.2 1 3136 60 133 3211 1.2 1 3151 60 134 2770 1.2 1 2694 76 135 2409 1.2 1 2334 75 136 2041 1.2 1 1986 55 137 1853 1.2 1 1823 30 138 2013 1.2 1 1973 40 139 1717 1.2 1 1676 41 140 1633 1.2 1 1595 38 141 2122 1.2 1 2084 38 142 1672 1.2 1 1631 41 143 1962 1.2 1 1912 50 144 2442 1.2 1 2291 151 145 3255 1.2 1 2930 325 146 1762 1.2 1 1547 215 147 246 1.2 1 197 49 148 5959 1.2 1 3695 2264 149 1793 1.2 1 1439 354 150 56368 1.2 1 47460 8908 RUN STATISTICS FOR INPUT FILE: SRR18245595_1.fastq.gz ============================================= 80869598 sequences processed in total