SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245594_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 1650). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245594_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4130.445 s (51.384 µs/read; 1.17 M reads/minute). === Summary === Total reads processed: 80,383,621 Reads with adapters: 30,854,033 (38.4%) Reads written (passing filters): 80,383,621 (100.0%) Total basepairs processed: 12,057,543,150 bp Quality-trimmed: 10,546,090 bp (0.1%) Total written (filtered): 11,995,107,122 bp (99.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 30854033 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.6% C: 0.5% G: 12.9% T: 48.9% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 25110487 20095905.2 0 25110487 2 3579993 5023976.3 0 3579993 3 1392096 1255994.1 0 1392096 4 601889 313998.5 0 601889 5 5323 78499.6 0 5323 6 4527 19624.9 0 4527 7 3387 4906.2 0 3387 8 3168 1226.6 0 3168 9 3914 306.6 0 3268 646 10 3906 76.7 1 2620 1286 11 3217 19.2 1 2763 454 12 2749 4.8 1 2523 226 13 2374 1.2 1 2249 125 14 2546 1.2 1 2332 214 15 2191 1.2 1 2073 118 16 2395 1.2 1 2159 236 17 2361 1.2 1 2211 150 18 1980 1.2 1 1895 85 19 1963 1.2 1 1829 134 20 1826 1.2 1 1722 104 21 1747 1.2 1 1620 127 22 1665 1.2 1 1594 71 23 1674 1.2 1 1578 96 24 1787 1.2 1 1604 183 25 1427 1.2 1 1356 71 26 1539 1.2 1 1417 122 27 1412 1.2 1 1340 72 28 1222 1.2 1 1163 59 29 1386 1.2 1 1268 118 30 1140 1.2 1 1072 68 31 1120 1.2 1 1049 71 32 1073 1.2 1 1023 50 33 1092 1.2 1 1003 89 34 1052 1.2 1 1006 46 35 1118 1.2 1 1003 115 36 1014 1.2 1 925 89 37 924 1.2 1 866 58 38 920 1.2 1 865 55 39 856 1.2 1 821 35 40 905 1.2 1 821 84 41 771 1.2 1 726 45 42 834 1.2 1 789 45 43 848 1.2 1 757 91 44 926 1.2 1 812 114 45 1063 1.2 1 1019 44 46 403 1.2 1 352 51 47 523 1.2 1 504 19 48 723 1.2 1 670 53 49 667 1.2 1 599 68 50 621 1.2 1 569 52 51 941 1.2 1 837 104 52 565 1.2 1 510 55 53 464 1.2 1 436 28 54 562 1.2 1 538 24 55 658 1.2 1 630 28 56 603 1.2 1 546 57 57 545 1.2 1 525 20 58 523 1.2 1 483 40 59 705 1.2 1 650 55 60 327 1.2 1 311 16 61 392 1.2 1 358 34 62 578 1.2 1 545 33 63 419 1.2 1 406 13 64 346 1.2 1 317 29 65 411 1.2 1 362 49 66 476 1.2 1 447 29 67 373 1.2 1 340 33 68 567 1.2 1 536 31 69 592 1.2 1 553 39 70 855 1.2 1 821 34 71 65 1.2 1 49 16 72 54 1.2 1 38 16 73 168 1.2 1 158 10 74 273 1.2 1 251 22 75 336 1.2 1 318 18 76 310 1.2 1 291 19 77 364 1.2 1 347 17 78 367 1.2 1 337 30 79 380 1.2 1 348 32 80 364 1.2 1 337 27 81 343 1.2 1 321 22 82 360 1.2 1 334 26 83 351 1.2 1 325 26 84 360 1.2 1 330 30 85 343 1.2 1 315 28 86 320 1.2 1 297 23 87 298 1.2 1 283 15 88 323 1.2 1 303 20 89 294 1.2 1 274 20 90 301 1.2 1 272 29 91 313 1.2 1 299 14 92 293 1.2 1 276 17 93 297 1.2 1 279 18 94 304 1.2 1 284 20 95 350 1.2 1 336 14 96 299 1.2 1 283 16 97 339 1.2 1 323 16 98 303 1.2 1 284 19 99 271 1.2 1 255 16 100 317 1.2 1 302 15 101 302 1.2 1 289 13 102 303 1.2 1 282 21 103 311 1.2 1 293 18 104 320 1.2 1 303 17 105 309 1.2 1 293 16 106 269 1.2 1 258 11 107 280 1.2 1 262 18 108 261 1.2 1 249 12 109 271 1.2 1 255 16 110 299 1.2 1 276 23 111 312 1.2 1 298 14 112 281 1.2 1 271 10 113 262 1.2 1 249 13 114 281 1.2 1 266 15 115 284 1.2 1 270 14 116 266 1.2 1 256 10 117 273 1.2 1 264 9 118 246 1.2 1 234 12 119 314 1.2 1 304 10 120 341 1.2 1 333 8 121 350 1.2 1 343 7 122 366 1.2 1 355 11 123 348 1.2 1 342 6 124 342 1.2 1 337 5 125 299 1.2 1 297 2 126 283 1.2 1 277 6 127 242 1.2 1 237 5 128 224 1.2 1 218 6 129 168 1.2 1 162 6 130 184 1.2 1 177 7 131 135 1.2 1 130 5 132 181 1.2 1 176 5 133 164 1.2 1 158 6 134 164 1.2 1 156 8 135 167 1.2 1 163 4 136 151 1.2 1 148 3 137 173 1.2 1 170 3 138 236 1.2 1 227 9 139 270 1.2 1 261 9 140 330 1.2 1 325 5 141 523 1.2 1 517 6 142 551 1.2 1 546 5 143 905 1.2 1 900 5 144 1402 1.2 1 1385 17 145 2764 1.2 1 2728 36 146 1975 1.2 1 1945 30 147 602 1.2 1 584 18 148 10131 1.2 1 10007 124 149 4035 1.2 1 3914 121 150 35877 1.2 1 34889 988 RUN STATISTICS FOR INPUT FILE: SRR18245594_1.fastq.gz ============================================= 80383621 sequences processed in total