SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245593_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 265304). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245593_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4541.119 s (44.974 µs/read; 1.33 M reads/minute). === Summary === Total reads processed: 100,971,775 Reads with adapters: 55,710,867 (55.2%) Reads written (passing filters): 100,971,775 (100.0%) Total basepairs processed: 15,145,766,250 bp Quality-trimmed: 17,876,800 bp (0.1%) Total written (filtered): 12,966,346,714 bp (85.6%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 55710867 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 16.4% C: 44.2% G: 6.6% T: 28.9% none/other: 3.8% Overview of removed sequences length count expect max.err error counts 1 20642519 25242943.8 0 20642519 2 3435921 6310735.9 0 3435921 3 1500980 1577684.0 0 1500980 4 875258 394421.0 0 875258 5 367741 98605.2 0 367741 6 328588 24651.3 0 328588 7 327926 6162.8 0 327926 8 346449 1540.7 0 346449 9 338904 385.2 0 338138 766 10 322548 96.3 1 313330 9218 11 346593 24.1 1 329037 17556 12 329051 6.0 1 317718 11333 13 307941 1.5 1 298464 9477 14 326144 1.5 1 310098 16046 15 306668 1.5 1 295974 10694 16 318326 1.5 1 301430 16896 17 322568 1.5 1 306586 15982 18 299818 1.5 1 290510 9308 19 276030 1.5 1 266102 9928 20 290026 1.5 1 279913 10113 21 286892 1.5 1 276046 10846 22 273485 1.5 1 265586 7899 23 281055 1.5 1 270527 10528 24 283984 1.5 1 268879 15105 25 262674 1.5 1 254073 8601 26 259295 1.5 1 250236 9059 27 261935 1.5 1 253202 8733 28 246028 1.5 1 238867 7161 29 259308 1.5 1 247207 12101 30 244142 1.5 1 237463 6679 31 228121 1.5 1 221105 7016 32 233960 1.5 1 227189 6771 33 231448 1.5 1 222645 8803 34 234109 1.5 1 225886 8223 35 214931 1.5 1 208298 6633 36 224899 1.5 1 216967 7932 37 211768 1.5 1 204637 7131 38 202062 1.5 1 197234 4828 39 208061 1.5 1 201379 6682 40 199878 1.5 1 194728 5150 41 216403 1.5 1 209696 6707 42 199624 1.5 1 192751 6873 43 206601 1.5 1 193758 12843 44 191013 1.5 1 183796 7217 45 281743 1.5 1 274946 6797 46 90745 1.5 1 87134 3611 47 164526 1.5 1 160676 3850 48 202761 1.5 1 197361 5400 49 157286 1.5 1 153411 3875 50 180093 1.5 1 173632 6461 51 200155 1.5 1 192663 7492 52 164211 1.5 1 160147 4064 53 143750 1.5 1 140824 2926 54 173929 1.5 1 168985 4944 55 190606 1.5 1 186545 4061 56 147656 1.5 1 143795 3861 57 194931 1.5 1 191325 3606 58 150274 1.5 1 146946 3328 59 162612 1.5 1 158356 4256 60 141909 1.5 1 139875 2034 61 100877 1.5 1 98322 2555 62 170219 1.5 1 166648 3571 63 187051 1.5 1 184536 2515 64 110672 1.5 1 108402 2270 65 114589 1.5 1 111766 2823 66 175457 1.5 1 172440 3017 67 151378 1.5 1 148182 3196 68 205760 1.5 1 201511 4249 69 199314 1.5 1 194037 5277 70 348540 1.5 1 340851 7689 71 19708 1.5 1 18177 1531 72 11278 1.5 1 10570 708 73 51718 1.5 1 50690 1028 74 100586 1.5 1 98890 1696 75 125340 1.5 1 123109 2231 76 132909 1.5 1 130408 2501 77 136485 1.5 1 133855 2630 78 138834 1.5 1 136039 2795 79 137886 1.5 1 134947 2939 80 138490 1.5 1 135605 2885 81 139921 1.5 1 136949 2972 82 139911 1.5 1 137045 2866 83 140406 1.5 1 137522 2884 84 141271 1.5 1 138457 2814 85 142431 1.5 1 139504 2927 86 141823 1.5 1 139001 2822 87 142600 1.5 1 139921 2679 88 142346 1.5 1 139525 2821 89 143863 1.5 1 141063 2800 90 144286 1.5 1 141363 2923 91 145001 1.5 1 142219 2782 92 145138 1.5 1 142250 2888 93 146708 1.5 1 143858 2850 94 147515 1.5 1 144785 2730 95 149975 1.5 1 147156 2819 96 150427 1.5 1 147694 2733 97 152413 1.5 1 149656 2757 98 153137 1.5 1 150472 2665 99 154683 1.5 1 152004 2679 100 155251 1.5 1 152424 2827 101 157491 1.5 1 154851 2640 102 159416 1.5 1 156661 2755 103 161206 1.5 1 158387 2819 104 161860 1.5 1 159283 2577 105 164393 1.5 1 161864 2529 106 165382 1.5 1 162753 2629 107 167330 1.5 1 164704 2626 108 168637 1.5 1 166012 2625 109 169763 1.5 1 167251 2512 110 172492 1.5 1 169870 2622 111 173928 1.5 1 171333 2595 112 175956 1.5 1 173327 2629 113 178661 1.5 1 175991 2670 114 181760 1.5 1 178999 2761 115 185255 1.5 1 182458 2797 116 193395 1.5 1 190644 2751 117 202078 1.5 1 199284 2794 118 216334 1.5 1 213367 2967 119 242382 1.5 1 239322 3060 120 269056 1.5 1 265627 3429 121 291021 1.5 1 287447 3574 122 293207 1.5 1 289579 3628 123 296182 1.5 1 292526 3656 124 285647 1.5 1 282318 3329 125 254028 1.5 1 248294 5734 126 221269 1.5 1 218484 2785 127 200028 1.5 1 197728 2300 128 184400 1.5 1 182211 2189 129 162381 1.5 1 160358 2023 130 145893 1.5 1 144081 1812 131 141714 1.5 1 139820 1894 132 130755 1.5 1 128866 1889 133 126333 1.5 1 124233 2100 134 110924 1.5 1 108712 2212 135 95303 1.5 1 92927 2376 136 82450 1.5 1 80666 1784 137 78165 1.5 1 76885 1280 138 84882 1.5 1 83484 1398 139 73176 1.5 1 71806 1370 140 73175 1.5 1 71817 1358 141 90056 1.5 1 88413 1643 142 74107 1.5 1 72736 1371 143 80889 1.5 1 78619 2270 144 109269 1.5 1 103606 5663 145 144577 1.5 1 133027 11550 146 80835 1.5 1 73162 7673 147 9399 1.5 1 7425 1974 148 242583 1.5 1 153614 88969 149 69540 1.5 1 57864 11676 150 2116845 1.5 1 1805851 310994 RUN STATISTICS FOR INPUT FILE: SRR18245593_1.fastq.gz ============================================= 100971775 sequences processed in total