SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245592_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 36615). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245592_1.fastq.gz Processing single-end reads on 1 core ... Finished in 3625.751 s (44.413 µs/read; 1.35 M reads/minute). === Summary === Total reads processed: 81,636,647 Reads with adapters: 32,937,505 (40.3%) Reads written (passing filters): 81,636,647 (100.0%) Total basepairs processed: 12,245,497,050 bp Quality-trimmed: 10,881,459 bp (0.1%) Total written (filtered): 12,048,909,694 bp (98.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 32937505 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 31.0% C: 12.2% G: 13.1% T: 43.5% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 22815814 20409161.8 0 22815814 2 3852809 5102290.4 0 3852809 3 1484438 1275572.6 0 1484438 4 719687 318893.2 0 719687 5 145459 79723.3 0 145459 6 130535 19930.8 0 130535 7 124886 4982.7 0 124886 8 129306 1245.7 0 129306 9 118080 311.4 0 117350 730 10 115779 77.9 1 110827 4952 11 121363 19.5 1 113321 8042 12 116273 4.9 1 110744 5529 13 100340 1.2 1 96421 3919 14 105730 1.2 1 98976 6754 15 97925 1.2 1 93545 4380 16 99243 1.2 1 92454 6789 17 96519 1.2 1 90720 5799 18 88628 1.2 1 84720 3908 19 80054 1.2 1 76250 3804 20 81683 1.2 1 77869 3814 21 79582 1.2 1 75514 4068 22 73142 1.2 1 70170 2972 23 75373 1.2 1 71530 3843 24 72382 1.2 1 67510 4872 25 65780 1.2 1 62886 2894 26 64509 1.2 1 61479 3030 27 63190 1.2 1 60359 2831 28 57224 1.2 1 55227 1997 29 57828 1.2 1 54374 3454 30 55607 1.2 1 53407 2200 31 49037 1.2 1 47009 2028 32 49852 1.2 1 48020 1832 33 48047 1.2 1 45389 2658 34 47392 1.2 1 45190 2202 35 42961 1.2 1 41147 1814 36 42335 1.2 1 40613 1722 37 40970 1.2 1 39173 1797 38 39495 1.2 1 37605 1890 39 36588 1.2 1 35307 1281 40 35211 1.2 1 33643 1568 41 34050 1.2 1 32715 1335 42 36176 1.2 1 34634 1542 43 33651 1.2 1 31763 1888 44 27839 1.2 1 26678 1161 45 45537 1.2 1 44001 1536 46 12002 1.2 1 11308 694 47 24850 1.2 1 24071 779 48 28697 1.2 1 27600 1097 49 22831 1.2 1 22068 763 50 24161 1.2 1 22992 1169 51 25010 1.2 1 23752 1258 52 23128 1.2 1 22292 836 53 19896 1.2 1 19316 580 54 21014 1.2 1 20231 783 55 22206 1.2 1 21403 803 56 17716 1.2 1 17039 677 57 21622 1.2 1 21002 620 58 18606 1.2 1 17975 631 59 16269 1.2 1 15564 705 60 17200 1.2 1 16810 390 61 12534 1.2 1 12076 458 62 16902 1.2 1 16334 568 63 18760 1.2 1 18369 391 64 12822 1.2 1 12424 398 65 11141 1.2 1 10719 422 66 16031 1.2 1 15607 424 67 16067 1.2 1 15542 525 68 20533 1.2 1 19921 612 69 16918 1.2 1 16245 673 70 27256 1.2 1 26297 959 71 1932 1.2 1 1732 200 72 1284 1.2 1 1194 90 73 5171 1.2 1 5044 127 74 8400 1.2 1 8188 212 75 9681 1.2 1 9423 258 76 10237 1.2 1 9952 285 77 10217 1.2 1 9912 305 78 10121 1.2 1 9815 306 79 9880 1.2 1 9567 313 80 9710 1.2 1 9403 307 81 9482 1.2 1 9163 319 82 9561 1.2 1 9270 291 83 8985 1.2 1 8700 285 84 9016 1.2 1 8747 269 85 8935 1.2 1 8633 302 86 8827 1.2 1 8544 283 87 8564 1.2 1 8307 257 88 8379 1.2 1 8149 230 89 8437 1.2 1 8174 263 90 8128 1.2 1 7849 279 91 8018 1.2 1 7731 287 92 7703 1.2 1 7447 256 93 7665 1.2 1 7441 224 94 7559 1.2 1 7333 226 95 7515 1.2 1 7301 214 96 7416 1.2 1 7198 218 97 7359 1.2 1 7153 206 98 7385 1.2 1 7159 226 99 7136 1.2 1 6925 211 100 7089 1.2 1 6882 207 101 7206 1.2 1 6993 213 102 7183 1.2 1 6961 222 103 6939 1.2 1 6749 190 104 6988 1.2 1 6812 176 105 7068 1.2 1 6863 205 106 6884 1.2 1 6694 190 107 6822 1.2 1 6608 214 108 6684 1.2 1 6490 194 109 6614 1.2 1 6440 174 110 6711 1.2 1 6509 202 111 6599 1.2 1 6417 182 112 6529 1.2 1 6353 176 113 6664 1.2 1 6491 173 114 6569 1.2 1 6383 186 115 6580 1.2 1 6398 182 116 6785 1.2 1 6641 144 117 6940 1.2 1 6763 177 118 7554 1.2 1 7365 189 119 8119 1.2 1 7964 155 120 9039 1.2 1 8843 196 121 9407 1.2 1 9213 194 122 9686 1.2 1 9490 196 123 9457 1.2 1 9266 191 124 8964 1.2 1 8800 164 125 7884 1.2 1 7676 208 126 6840 1.2 1 6720 120 127 6145 1.2 1 6045 100 128 5434 1.2 1 5331 103 129 4544 1.2 1 4466 78 130 4166 1.2 1 4094 72 131 4025 1.2 1 3955 70 132 3692 1.2 1 3636 56 133 3484 1.2 1 3414 70 134 3145 1.2 1 3063 82 135 2573 1.2 1 2498 75 136 2305 1.2 1 2221 84 137 2128 1.2 1 2086 42 138 2471 1.2 1 2423 48 139 2031 1.2 1 1980 51 140 1952 1.2 1 1913 39 141 2506 1.2 1 2460 46 142 1954 1.2 1 1906 48 143 2181 1.2 1 2127 54 144 2717 1.2 1 2576 141 145 3734 1.2 1 3400 334 146 1916 1.2 1 1736 180 147 252 1.2 1 196 56 148 5605 1.2 1 3604 2001 149 1560 1.2 1 1221 339 150 51997 1.2 1 43677 8320 RUN STATISTICS FOR INPUT FILE: SRR18245592_1.fastq.gz ============================================= 81636647 sequences processed in total