SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245591_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 51919). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245591_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4660.320 s (56.744 µs/read; 1.06 M reads/minute). === Summary === Total reads processed: 82,128,972 Reads with adapters: 34,457,448 (42.0%) Reads written (passing filters): 82,128,972 (100.0%) Total basepairs processed: 12,319,345,800 bp Quality-trimmed: 16,928,036 bp (0.1%) Total written (filtered): 12,049,107,926 bp (97.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 34457448 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 30.6% C: 15.2% G: 11.7% T: 42.5% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 23120248 20532243.0 0 23120248 2 3710916 5133060.8 0 3710916 3 1483347 1283265.2 0 1483347 4 702451 320816.3 0 702451 5 149836 80204.1 0 149836 6 132083 20051.0 0 132083 7 126948 5012.8 0 126948 8 137642 1253.2 0 137642 9 124419 313.3 0 123631 788 10 121163 78.3 1 116592 4571 11 130222 19.6 1 122382 7840 12 120764 4.9 1 115968 4796 13 113046 1.2 1 109044 4002 14 115749 1.2 1 109639 6110 15 110468 1.2 1 105964 4504 16 110850 1.2 1 104532 6318 17 112955 1.2 1 106644 6311 18 104068 1.2 1 100487 3581 19 90858 1.2 1 87495 3363 20 97482 1.2 1 93546 3936 21 94760 1.2 1 90802 3958 22 87396 1.2 1 84560 2836 23 91736 1.2 1 87685 4051 24 89909 1.2 1 84671 5238 25 83795 1.2 1 80616 3179 26 80959 1.2 1 77790 3169 27 81148 1.2 1 77966 3182 28 75584 1.2 1 73088 2496 29 79167 1.2 1 74895 4272 30 73321 1.2 1 71069 2252 31 67107 1.2 1 64771 2336 32 67965 1.2 1 65785 2180 33 67097 1.2 1 64270 2827 34 64523 1.2 1 62340 2183 35 67433 1.2 1 63807 3626 36 60290 1.2 1 58425 1865 37 59205 1.2 1 56998 2207 38 63181 1.2 1 61437 1744 39 53571 1.2 1 51192 2379 40 56738 1.2 1 55166 1572 41 48952 1.2 1 46706 2246 42 50460 1.2 1 49158 1302 43 55779 1.2 1 53005 2774 44 50674 1.2 1 48360 2314 45 69401 1.2 1 67665 1736 46 25063 1.2 1 23877 1186 47 42433 1.2 1 41355 1078 48 46956 1.2 1 45565 1391 49 41392 1.2 1 40258 1134 50 43739 1.2 1 42048 1691 51 44435 1.2 1 42433 2002 52 41684 1.2 1 40415 1269 53 38086 1.2 1 37077 1009 54 38478 1.2 1 37243 1235 55 40803 1.2 1 39625 1178 56 34737 1.2 1 33703 1034 57 39019 1.2 1 37866 1153 58 40218 1.2 1 38981 1237 59 29810 1.2 1 28731 1079 60 34811 1.2 1 34067 744 61 29551 1.2 1 28703 848 62 33742 1.2 1 32794 948 63 35166 1.2 1 34376 790 64 32190 1.2 1 31363 827 65 24118 1.2 1 23423 695 66 30858 1.2 1 30129 729 67 35573 1.2 1 34483 1090 68 44589 1.2 1 43335 1254 69 37985 1.2 1 36588 1397 70 72747 1.2 1 70654 2093 71 3395 1.2 1 3053 342 72 1711 1.2 1 1542 169 73 8825 1.2 1 8557 268 74 17934 1.2 1 17517 417 75 22085 1.2 1 21552 533 76 22990 1.2 1 22409 581 77 23457 1.2 1 22834 623 78 23205 1.2 1 22568 637 79 22938 1.2 1 22295 643 80 22724 1.2 1 22076 648 81 22129 1.2 1 21474 655 82 21488 1.2 1 20881 607 83 21378 1.2 1 20766 612 84 21028 1.2 1 20389 639 85 20675 1.2 1 20012 663 86 20054 1.2 1 19463 591 87 19340 1.2 1 18760 580 88 19222 1.2 1 18625 597 89 18620 1.2 1 18044 576 90 18531 1.2 1 17950 581 91 18003 1.2 1 17474 529 92 17543 1.2 1 16940 603 93 17137 1.2 1 16576 561 94 16528 1.2 1 16033 495 95 16248 1.2 1 15704 544 96 16198 1.2 1 15624 574 97 15391 1.2 1 14882 509 98 15094 1.2 1 14549 545 99 14885 1.2 1 14418 467 100 14248 1.2 1 13734 514 101 14058 1.2 1 13613 445 102 13375 1.2 1 12900 475 103 13275 1.2 1 12795 480 104 12812 1.2 1 12337 475 105 12471 1.2 1 12037 434 106 12043 1.2 1 11569 474 107 11728 1.2 1 11301 427 108 11218 1.2 1 10782 436 109 10913 1.2 1 10534 379 110 10544 1.2 1 10156 388 111 10230 1.2 1 9857 373 112 10012 1.2 1 9639 373 113 9555 1.2 1 9181 374 114 9275 1.2 1 8896 379 115 8952 1.2 1 8643 309 116 8894 1.2 1 8519 375 117 8448 1.2 1 8153 295 118 8458 1.2 1 8141 317 119 8020 1.2 1 7770 250 120 7853 1.2 1 7621 232 121 7806 1.2 1 7576 230 122 7620 1.2 1 7403 217 123 6841 1.2 1 6623 218 124 6405 1.2 1 6226 179 125 5753 1.2 1 5608 145 126 5032 1.2 1 4885 147 127 4571 1.2 1 4476 95 128 4170 1.2 1 4073 97 129 3483 1.2 1 3390 93 130 3095 1.2 1 3029 66 131 2894 1.2 1 2831 63 132 2447 1.2 1 2385 62 133 2188 1.2 1 2143 45 134 1928 1.2 1 1891 37 135 1541 1.2 1 1506 35 136 1256 1.2 1 1216 40 137 1128 1.2 1 1099 29 138 1182 1.2 1 1158 24 139 1010 1.2 1 984 26 140 1072 1.2 1 1042 30 141 1275 1.2 1 1249 26 142 1085 1.2 1 1055 30 143 950 1.2 1 929 21 144 1468 1.2 1 1385 83 145 1906 1.2 1 1763 143 146 913 1.2 1 834 79 147 127 1.2 1 111 16 148 1112 1.2 1 766 346 149 782 1.2 1 657 125 150 17421 1.2 1 14675 2746 RUN STATISTICS FOR INPUT FILE: SRR18245591_1.fastq.gz ============================================= 82128972 sequences processed in total