Bismark report for: SRR18245590_val_1.fq.gz and SRR18245590_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 88511239 Number of paired-end alignments with a unique best hit: 49816429 Mapping efficiency: 56.3% Sequence pairs with no alignments under any condition: 26420754 Sequence pairs did not map uniquely: 12274056 Sequence pairs which were discarded because genomic sequence could not be extracted: 18 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 24917052 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 24899359 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2285570673 Total methylated C's in CpG context: 35797553 Total methylated C's in CHG context: 11066331 Total methylated C's in CHH context: 48178758 Total methylated C's in Unknown context: 1583876 Total unmethylated C's in CpG context: 275628421 Total unmethylated C's in CHG context: 394227871 Total unmethylated C's in CHH context: 1520671739 Total unmethylated C's in Unknown context: 9323120 C methylated in CpG context: 11.5% C methylated in CHG context: 2.7% C methylated in CHH context: 3.1% C methylated in Unknown context (CN or CHN): 14.5% Bismark completed in 0d 11h 14m 11s