Bismark report for: SRR18245588_val_1.fq.gz and SRR18245588_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_03/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 70678740 Number of paired-end alignments with a unique best hit: 40193168 Mapping efficiency: 56.9% Sequence pairs with no alignments under any condition: 24224749 Sequence pairs did not map uniquely: 6260823 Sequence pairs which were discarded because genomic sequence could not be extracted: 18 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20099522 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 20093628 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1968029247 Total methylated C's in CpG context: 30371612 Total methylated C's in CHG context: 8392880 Total methylated C's in CHH context: 37582319 Total methylated C's in Unknown context: 1249874 Total unmethylated C's in CpG context: 212368543 Total unmethylated C's in CHG context: 325366962 Total unmethylated C's in CHH context: 1353946931 Total unmethylated C's in Unknown context: 7480456 C methylated in CpG context: 12.5% C methylated in CHG context: 2.5% C methylated in CHH context: 2.7% C methylated in Unknown context (CN or CHN): 14.3% Bismark completed in 0d 8h 53m 14s