SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245587_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 4659). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245587_1.fastq.gz Processing single-end reads on 1 core ... Finished in 3095.265 s (45.908 µs/read; 1.31 M reads/minute). === Summary === Total reads processed: 67,423,445 Reads with adapters: 25,956,345 (38.5%) Reads written (passing filters): 67,423,445 (100.0%) Total basepairs processed: 10,113,516,750 bp Quality-trimmed: 7,649,719 bp (0.1%) Total written (filtered): 10,044,935,622 bp (99.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 25956345 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 37.1% C: 1.2% G: 12.9% T: 48.6% none/other: 0.3% Overview of removed sequences length count expect max.err error counts 1 20890514 16855861.2 0 20890514 2 3016256 4213965.3 0 3016256 3 1166060 1053491.3 0 1166060 4 506940 263372.8 0 506940 5 8926 65843.2 0 8926 6 7127 16460.8 0 7127 7 6283 4115.2 0 6283 8 6209 1028.8 0 6209 9 6860 257.2 0 6322 538 10 6477 64.3 1 5291 1186 11 6529 16.1 1 5910 619 12 5663 4.0 1 5386 277 13 4863 1.0 1 4661 202 14 5381 1.0 1 4961 420 15 4845 1.0 1 4632 213 16 5284 1.0 1 4877 407 17 4896 1.0 1 4585 311 18 4380 1.0 1 4201 179 19 4266 1.0 1 4054 212 20 4023 1.0 1 3840 183 21 4142 1.0 1 3921 221 22 3667 1.0 1 3523 144 23 3711 1.0 1 3511 200 24 4112 1.0 1 3818 294 25 3524 1.0 1 3391 133 26 3478 1.0 1 3297 181 27 3419 1.0 1 3284 135 28 3085 1.0 1 2964 121 29 3448 1.0 1 3219 229 30 3033 1.0 1 2926 107 31 2863 1.0 1 2741 122 32 2852 1.0 1 2744 108 33 2906 1.0 1 2713 193 34 2816 1.0 1 2680 136 35 2642 1.0 1 2504 138 36 2506 1.0 1 2403 103 37 2394 1.0 1 2313 81 38 2399 1.0 1 2268 131 39 2564 1.0 1 2378 186 40 2180 1.0 1 2074 106 41 2208 1.0 1 2104 104 42 2404 1.0 1 2318 86 43 2145 1.0 1 1975 170 44 2349 1.0 1 2201 148 45 2857 1.0 1 2757 100 46 1126 1.0 1 1065 61 47 1667 1.0 1 1633 34 48 2017 1.0 1 1941 76 49 1818 1.0 1 1748 70 50 1842 1.0 1 1745 97 51 2165 1.0 1 2030 135 52 1668 1.0 1 1606 62 53 1552 1.0 1 1490 62 54 1690 1.0 1 1634 56 55 1680 1.0 1 1609 71 56 1499 1.0 1 1439 60 57 1585 1.0 1 1535 50 58 1583 1.0 1 1513 70 59 1779 1.0 1 1693 86 60 1227 1.0 1 1203 24 61 1347 1.0 1 1287 60 62 1533 1.0 1 1475 58 63 1495 1.0 1 1450 45 64 1371 1.0 1 1318 53 65 1330 1.0 1 1270 60 66 1429 1.0 1 1375 54 67 1336 1.0 1 1280 56 68 1906 1.0 1 1826 80 69 1901 1.0 1 1814 87 70 3243 1.0 1 3137 106 71 176 1.0 1 147 29 72 140 1.0 1 113 27 73 547 1.0 1 522 25 74 913 1.0 1 887 26 75 1091 1.0 1 1062 29 76 1114 1.0 1 1078 36 77 1080 1.0 1 1039 41 78 1084 1.0 1 1047 37 79 1093 1.0 1 1057 36 80 1106 1.0 1 1075 31 81 1101 1.0 1 1060 41 82 973 1.0 1 936 37 83 1050 1.0 1 1016 34 84 1001 1.0 1 963 38 85 1026 1.0 1 992 34 86 1063 1.0 1 1025 38 87 996 1.0 1 969 27 88 923 1.0 1 892 31 89 960 1.0 1 929 31 90 1025 1.0 1 997 28 91 924 1.0 1 899 25 92 940 1.0 1 907 33 93 956 1.0 1 921 35 94 906 1.0 1 877 29 95 945 1.0 1 923 22 96 861 1.0 1 829 32 97 911 1.0 1 885 26 98 908 1.0 1 881 27 99 803 1.0 1 778 25 100 851 1.0 1 826 25 101 837 1.0 1 808 29 102 832 1.0 1 810 22 103 828 1.0 1 801 27 104 807 1.0 1 779 28 105 719 1.0 1 700 19 106 761 1.0 1 743 18 107 755 1.0 1 725 30 108 763 1.0 1 740 23 109 731 1.0 1 713 18 110 668 1.0 1 642 26 111 687 1.0 1 668 19 112 670 1.0 1 646 24 113 647 1.0 1 622 25 114 653 1.0 1 632 21 115 627 1.0 1 615 12 116 613 1.0 1 599 14 117 611 1.0 1 601 10 118 622 1.0 1 607 15 119 670 1.0 1 650 20 120 801 1.0 1 790 11 121 817 1.0 1 798 19 122 804 1.0 1 784 20 123 837 1.0 1 828 9 124 790 1.0 1 778 12 125 706 1.0 1 697 9 126 737 1.0 1 719 18 127 578 1.0 1 568 10 128 509 1.0 1 502 7 129 484 1.0 1 469 15 130 430 1.0 1 423 7 131 405 1.0 1 397 8 132 427 1.0 1 417 10 133 456 1.0 1 446 10 134 430 1.0 1 423 7 135 371 1.0 1 364 7 136 425 1.0 1 418 7 137 436 1.0 1 422 14 138 584 1.0 1 574 10 139 678 1.0 1 671 7 140 849 1.0 1 843 6 141 1337 1.0 1 1323 14 142 1563 1.0 1 1556 7 143 2098 1.0 1 2081 17 144 2885 1.0 1 2854 31 145 5219 1.0 1 5148 71 146 3289 1.0 1 3265 24 147 1077 1.0 1 1056 21 148 20428 1.0 1 20159 269 149 7791 1.0 1 7643 148 150 69861 1.0 1 68388 1473 RUN STATISTICS FOR INPUT FILE: SRR18245587_1.fastq.gz ============================================= 67423445 sequences processed in total