SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245586_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 86040). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245586_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4190.203 s (50.660 µs/read; 1.18 M reads/minute). === Summary === Total reads processed: 82,712,035 Reads with adapters: 36,315,231 (43.9%) Reads written (passing filters): 82,712,035 (100.0%) Total basepairs processed: 12,406,805,250 bp Quality-trimmed: 14,673,587 bp (0.1%) Total written (filtered): 12,044,338,266 bp (97.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 36315231 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 24.8% C: 26.2% G: 10.7% T: 38.1% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 20780690 20678008.8 0 20780690 2 3589466 5169502.2 0 3589466 3 1515991 1292375.5 0 1515991 4 840991 323093.9 0 840991 5 342257 80773.5 0 342257 6 300766 20193.4 0 300766 7 289751 5048.3 0 289751 8 303650 1262.1 0 303650 9 286482 315.5 0 285643 839 10 272331 78.9 1 263312 9019 11 290805 19.7 1 273989 16816 12 267567 4.9 1 257344 10223 13 249562 1.2 1 240809 8753 14 256279 1.2 1 242874 13405 15 240463 1.2 1 230959 9504 16 242290 1.2 1 228634 13656 17 242248 1.2 1 228846 13402 18 221097 1.2 1 213572 7525 19 194129 1.2 1 186756 7373 20 206887 1.2 1 198335 8552 21 197913 1.2 1 189645 8268 22 182754 1.2 1 176888 5866 23 189841 1.2 1 181571 8270 24 180305 1.2 1 170068 10237 25 172163 1.2 1 165245 6918 26 160137 1.2 1 153832 6305 27 161121 1.2 1 154224 6897 28 153776 1.2 1 148211 5565 29 147344 1.2 1 139686 7658 30 141016 1.2 1 136362 4654 31 124772 1.2 1 120558 4214 32 133533 1.2 1 128971 4562 33 119389 1.2 1 114154 5235 34 129291 1.2 1 123999 5292 35 143206 1.2 1 139483 3723 36 68789 1.2 1 65340 3449 37 114214 1.2 1 108119 6095 38 101077 1.2 1 96554 4523 39 94117 1.2 1 91162 2955 40 91579 1.2 1 88321 3258 41 86536 1.2 1 83820 2716 42 89985 1.2 1 86809 3176 43 91871 1.2 1 87317 4554 44 71792 1.2 1 68957 2835 45 113528 1.2 1 110230 3298 46 34972 1.2 1 33174 1798 47 64904 1.2 1 63140 1764 48 73658 1.2 1 71344 2314 49 56218 1.2 1 54487 1731 50 64854 1.2 1 62115 2739 51 63590 1.2 1 60890 2700 52 54934 1.2 1 53269 1665 53 52316 1.2 1 50910 1406 54 56246 1.2 1 54172 2074 55 55452 1.2 1 53883 1569 56 43666 1.2 1 42296 1370 57 56224 1.2 1 54693 1531 58 45521 1.2 1 44136 1385 59 40271 1.2 1 38782 1489 60 45970 1.2 1 45056 914 61 30275 1.2 1 29276 999 62 41018 1.2 1 39778 1240 63 47489 1.2 1 46535 954 64 31598 1.2 1 30743 855 65 27932 1.2 1 27033 899 66 35147 1.2 1 34245 902 67 36268 1.2 1 35164 1104 68 43887 1.2 1 42521 1366 69 41380 1.2 1 39757 1623 70 83130 1.2 1 80694 2436 71 4804 1.2 1 4322 482 72 2180 1.2 1 1964 216 73 9914 1.2 1 9631 283 74 18954 1.2 1 18497 457 75 23009 1.2 1 22454 555 76 23677 1.2 1 23034 643 77 23707 1.2 1 23075 632 78 23132 1.2 1 22436 696 79 22749 1.2 1 22027 722 80 22183 1.2 1 21515 668 81 21364 1.2 1 20731 633 82 20911 1.2 1 20240 671 83 20145 1.2 1 19496 649 84 19598 1.2 1 18994 604 85 19293 1.2 1 18685 608 86 18510 1.2 1 17893 617 87 18305 1.2 1 17700 605 88 17539 1.2 1 17005 534 89 17173 1.2 1 16644 529 90 16606 1.2 1 16055 551 91 16511 1.2 1 15984 527 92 16032 1.2 1 15489 543 93 15171 1.2 1 14694 477 94 15257 1.2 1 14748 509 95 14790 1.2 1 14282 508 96 14128 1.2 1 13660 468 97 14169 1.2 1 13707 462 98 13419 1.2 1 12968 451 99 13194 1.2 1 12765 429 100 12591 1.2 1 12156 435 101 12507 1.2 1 12058 449 102 12441 1.2 1 11983 458 103 12102 1.2 1 11710 392 104 11535 1.2 1 11106 429 105 11234 1.2 1 10819 415 106 11230 1.2 1 10832 398 107 10767 1.2 1 10375 392 108 10525 1.2 1 10154 371 109 10397 1.2 1 10035 362 110 10110 1.2 1 9779 331 111 9894 1.2 1 9554 340 112 9615 1.2 1 9312 303 113 9496 1.2 1 9189 307 114 9329 1.2 1 8999 330 115 8887 1.2 1 8588 299 116 9228 1.2 1 8942 286 117 9165 1.2 1 8873 292 118 9065 1.2 1 8803 262 119 9611 1.2 1 9357 254 120 10008 1.2 1 9737 271 121 9945 1.2 1 9709 236 122 9955 1.2 1 9724 231 123 9607 1.2 1 9401 206 124 9133 1.2 1 8923 210 125 8164 1.2 1 7935 229 126 7158 1.2 1 7038 120 127 6478 1.2 1 6367 111 128 5842 1.2 1 5711 131 129 5328 1.2 1 5234 94 130 4822 1.2 1 4735 87 131 4625 1.2 1 4539 86 132 4011 1.2 1 3944 67 133 3945 1.2 1 3879 66 134 3357 1.2 1 3283 74 135 2981 1.2 1 2912 69 136 2533 1.2 1 2437 96 137 2300 1.2 1 2221 79 138 2460 1.2 1 2355 105 139 2098 1.2 1 1995 103 140 2077 1.2 1 1985 92 141 2474 1.2 1 2342 132 142 1997 1.2 1 1892 105 143 2017 1.2 1 1862 155 144 2702 1.2 1 2450 252 145 3287 1.2 1 2851 436 146 1760 1.2 1 1539 221 147 186 1.2 1 150 36 148 4073 1.2 1 2456 1617 149 1634 1.2 1 1315 319 150 51450 1.2 1 42550 8900 RUN STATISTICS FOR INPUT FILE: SRR18245586_1.fastq.gz ============================================= 82712035 sequences processed in total