SUMMARISING RUN PARAMETERS ========================== Input filename: SRR18245583_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 109052). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR18245583_1.fastq.gz Processing single-end reads on 1 core ... Finished in 4951.677 s (56.179 µs/read; 1.07 M reads/minute). === Summary === Total reads processed: 88,140,600 Reads with adapters: 40,282,503 (45.7%) Reads written (passing filters): 88,140,600 (100.0%) Total basepairs processed: 13,221,090,000 bp Quality-trimmed: 16,463,597 bp (0.1%) Total written (filtered): 12,707,161,273 bp (96.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 40282503 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 23.5% C: 30.1% G: 9.6% T: 36.6% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 21715639 22035150.0 0 21715639 2 3669363 5508787.5 0 3669363 3 1559285 1377196.9 0 1559285 4 912627 344299.2 0 912627 5 382544 86074.8 0 382544 6 333200 21518.7 0 333200 7 328955 5379.7 0 328955 8 342847 1344.9 0 342847 9 331064 336.2 0 330224 840 10 314373 84.1 1 302785 11588 11 335416 21.0 1 315450 19966 12 312677 5.3 1 298826 13851 13 288334 1.3 1 276886 11448 14 300274 1.3 1 282773 17501 15 280124 1.3 1 267698 12426 16 287165 1.3 1 269411 17754 17 285059 1.3 1 268380 16679 18 262983 1.3 1 252535 10448 19 235539 1.3 1 224874 10665 20 249620 1.3 1 238287 11333 21 237262 1.3 1 225962 11300 22 223680 1.3 1 215202 8478 23 227819 1.3 1 217198 10621 24 224388 1.3 1 210421 13967 25 208509 1.3 1 199683 8826 26 200545 1.3 1 191729 8816 27 200201 1.3 1 191564 8637 28 184682 1.3 1 177763 6919 29 186965 1.3 1 176586 10379 30 178542 1.3 1 171927 6615 31 159632 1.3 1 153231 6401 32 164180 1.3 1 157846 6334 33 158436 1.3 1 150441 7995 34 158433 1.3 1 150819 7614 35 142397 1.3 1 136685 5712 36 143797 1.3 1 137712 6085 37 134251 1.3 1 128556 5695 38 137407 1.3 1 130968 6439 39 121444 1.3 1 117244 4200 40 122905 1.3 1 117357 5548 41 116953 1.3 1 112404 4549 42 123283 1.3 1 117987 5296 43 122736 1.3 1 116109 6627 44 96630 1.3 1 92510 4120 45 156787 1.3 1 151805 4982 46 47206 1.3 1 44559 2647 47 85228 1.3 1 82737 2491 48 100502 1.3 1 96776 3726 49 80940 1.3 1 78112 2828 50 88744 1.3 1 84628 4116 51 89800 1.3 1 85465 4335 52 79191 1.3 1 76360 2831 53 72673 1.3 1 70432 2241 54 77341 1.3 1 74212 3129 55 79471 1.3 1 76787 2684 56 65634 1.3 1 63180 2454 57 76645 1.3 1 74242 2403 58 67620 1.3 1 65235 2385 59 63041 1.3 1 60545 2496 60 62360 1.3 1 60826 1534 61 48446 1.3 1 46631 1815 62 62765 1.3 1 60610 2155 63 67153 1.3 1 65458 1695 64 49654 1.3 1 48124 1530 65 43984 1.3 1 42352 1632 66 59005 1.3 1 57405 1600 67 56691 1.3 1 54813 1878 68 72649 1.3 1 70345 2304 69 64044 1.3 1 61476 2568 70 104985 1.3 1 101280 3705 71 6498 1.3 1 5896 602 72 4944 1.3 1 4585 359 73 20573 1.3 1 20042 531 74 32148 1.3 1 31360 788 75 37016 1.3 1 36035 981 76 37535 1.3 1 36457 1078 77 37541 1.3 1 36505 1036 78 36755 1.3 1 35614 1141 79 36318 1.3 1 35184 1134 80 35890 1.3 1 34755 1135 81 34915 1.3 1 33882 1033 82 34207 1.3 1 33119 1088 83 33464 1.3 1 32363 1101 84 32580 1.3 1 31608 972 85 32366 1.3 1 31400 966 86 31516 1.3 1 30515 1001 87 30761 1.3 1 29823 938 88 30182 1.3 1 29205 977 89 29194 1.3 1 28267 927 90 28593 1.3 1 27738 855 91 27950 1.3 1 27049 901 92 27618 1.3 1 26697 921 93 27095 1.3 1 26282 813 94 26477 1.3 1 25603 874 95 26498 1.3 1 25662 836 96 25820 1.3 1 25033 787 97 25353 1.3 1 24617 736 98 24787 1.3 1 24082 705 99 24225 1.3 1 23477 748 100 23946 1.3 1 23225 721 101 23237 1.3 1 22565 672 102 22954 1.3 1 22279 675 103 22792 1.3 1 22106 686 104 22521 1.3 1 21857 664 105 22645 1.3 1 21992 653 106 21818 1.3 1 21170 648 107 21707 1.3 1 21075 632 108 21373 1.3 1 20708 665 109 20805 1.3 1 20227 578 110 20202 1.3 1 19590 612 111 20166 1.3 1 19577 589 112 20252 1.3 1 19689 563 113 20226 1.3 1 19650 576 114 19817 1.3 1 19279 538 115 19497 1.3 1 19005 492 116 19802 1.3 1 19269 533 117 19474 1.3 1 18957 517 118 19974 1.3 1 19492 482 119 20357 1.3 1 19904 453 120 21201 1.3 1 20772 429 121 21594 1.3 1 21122 472 122 21504 1.3 1 21049 455 123 21097 1.3 1 20682 415 124 19878 1.3 1 19509 369 125 18006 1.3 1 17533 473 126 15867 1.3 1 15547 320 127 14619 1.3 1 14341 278 128 13390 1.3 1 13133 257 129 11861 1.3 1 11640 221 130 10709 1.3 1 10507 202 131 10445 1.3 1 10277 168 132 9313 1.3 1 9151 162 133 8721 1.3 1 8558 163 134 7686 1.3 1 7506 180 135 6575 1.3 1 6417 158 136 5655 1.3 1 5516 139 137 5358 1.3 1 5254 104 138 5774 1.3 1 5641 133 139 5240 1.3 1 5132 108 140 5497 1.3 1 5390 107 141 6864 1.3 1 6712 152 142 5454 1.3 1 5342 112 143 5418 1.3 1 5275 143 144 7696 1.3 1 7338 358 145 10008 1.3 1 9403 605 146 5001 1.3 1 4631 370 147 435 1.3 1 353 82 148 7648 1.3 1 5114 2534 149 2737 1.3 1 2199 538 150 84144 1.3 1 70645 13499 RUN STATISTICS FOR INPUT FILE: SRR18245583_1.fastq.gz ============================================= 88140600 sequences processed in total