##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065375_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81453846
Total Bases	12.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.76380329542696	32.0	32.0	32.0	32.0	32.0
2	31.062662615096162	32.0	32.0	32.0	32.0	32.0
3	34.82905106776665	37.0	32.0	37.0	32.0	37.0
4	35.549659459910586	37.0	37.0	37.0	32.0	37.0
5	35.72840269568118	37.0	37.0	37.0	32.0	37.0
6	39.25066195155475	41.0	41.0	41.0	37.0	41.0
7	39.385630311428145	41.0	41.0	41.0	37.0	41.0
8	39.46466457826927	41.0	41.0	41.0	37.0	41.0
9	39.475017754226116	41.0	41.0	41.0	37.0	41.0
10-14	39.50611355048845	41.0	41.0	41.0	37.0	41.0
15-19	39.4359614523297	41.0	41.0	41.0	37.0	41.0
20-24	39.37305769208246	41.0	41.0	41.0	37.0	41.0
25-29	39.29256913172645	41.0	41.0	41.0	37.0	41.0
30-34	39.227296115643206	41.0	41.0	41.0	37.0	41.0
35-39	39.15999765560487	41.0	41.0	41.0	37.0	41.0
40-44	39.08652466084904	41.0	41.0	41.0	37.0	41.0
45-49	38.9920326193069	41.0	41.0	41.0	37.0	41.0
50-54	38.85423261168048	41.0	41.0	41.0	37.0	41.0
55-59	38.740348790896874	41.0	41.0	41.0	36.0	41.0
60-64	38.65410911106641	41.0	41.0	41.0	32.0	41.0
65-69	38.53916149029967	41.0	41.0	41.0	32.0	41.0
70-74	38.42401363098312	41.0	41.0	41.0	32.0	41.0
75-79	38.21816847543331	41.0	39.4	41.0	31.0	41.0
80-84	38.37197140574553	41.0	41.0	41.0	32.0	41.0
85-89	38.26059786790178	41.0	41.0	41.0	32.0	41.0
90-94	38.11716189067364	41.0	41.0	41.0	32.0	41.0
95-99	37.922921925626454	41.0	38.6	41.0	29.0	41.0
100-104	37.6913026132615	41.0	37.0	41.0	27.0	41.0
105-109	37.50151725923414	41.0	37.0	41.0	27.0	41.0
110-114	37.26493066024163	41.0	37.0	41.0	27.0	41.0
115-119	36.967296382788355	41.0	37.0	41.0	27.0	41.0
120-124	36.64673357474121	41.0	37.0	41.0	23.0	41.0
125-129	36.33545220934074	41.0	37.0	41.0	22.0	41.0
130-134	35.93724005616629	41.0	36.0	41.0	22.0	41.0
135-139	35.481688015075434	41.0	32.0	41.0	22.0	41.0
140-144	34.96152562028808	39.4	32.0	41.0	22.0	41.0
145-149	34.38694652920379	37.0	32.0	41.0	14.0	41.0
150	34.010192937973734	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	76.0
12	569.0
13	2236.0
14	5689.0
15	13289.0
16	23243.0
17	34777.0
18	51325.0
19	80940.0
20	134599.0
21	221581.0
22	327088.0
23	430678.0
24	529423.0
25	633904.0
26	752437.0
27	885904.0
28	1032629.0
29	1188230.0
30	1344658.0
31	1484740.0
32	1615694.0
33	1761831.0
34	1997060.0
35	2434973.0
36	3224549.0
37	4640230.0
38	7477110.0
39	1.4903676E7
40	3.4220708E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.1397177016845625	51.98632079870136	30.852355024321355	17.02160647529273
2	0.13466960130085634	53.51237847917085	33.39526811537436	12.957683804153938
3	0.1924208584937409	48.631747148296164	35.036346489548265	16.13948550366183
4	0.23470069663893872	47.82026571248988	35.09689555623796	16.84813803463322
5	0.26266405084710054	48.37324771332289	34.7593964291185	16.604691806711518
6	0.2645919089862173	48.192219653787504	34.771542188596605	16.771646248629672
7	0.2606172292467628	48.75592321929464	34.45133235572459	16.532127195734013
8	0.24731551656873266	47.31223372804275	35.75054147842203	16.689909276966493
9	0.26971110416047883	47.49173154197395	35.75666880010454	16.48188855376103
10-14	0.25408864480853954	48.11180978777723	35.319567737494175	16.314533829920048
15-19	0.2702283574770886	47.80862231159919	35.51997834505774	16.40117098586599
20-24	0.2835581701159365	47.92653302780759	35.229420960333734	16.560487841742745
25-29	0.2897429816693347	47.92760115456986	35.12054251997457	16.662113343786235
30-34	0.28962714410858964	47.97451957650982	35.023469188469676	16.712384090911915
35-39	0.28883263227135547	47.98161170351646	35.01142144819099	16.7181342160212
40-44	0.2969292902156208	47.9856704624926	34.971177189528106	16.746223057763675
45-49	0.29962121650285	47.9658781564433	34.98383796397002	16.750662663083826
50-54	0.30287785376963955	47.98120999442555	34.966853169883336	16.749058981921472
55-59	0.30868984941740096	47.975318689930155	34.954983259225955	16.76100820142649
60-64	0.30979394463048104	47.94763116906704	34.95351068800269	16.789064198299787
65-69	0.31925218147751	47.93315093877936	34.9457561616281	16.801840718115034
70-74	0.32997574391160317	47.90936511183713	34.940940171199564	16.819718973051707
75-79	0.3287492263610484	47.89448048416404	34.950116585419536	16.82665370405537
80-84	0.34703780952680324	47.874853927466326	34.94140109720269	16.83670716580418
85-89	0.3444970525642749	47.875075842956015	34.936469812712886	16.843957291766827
90-94	0.3483685506164195	47.84219891213864	34.937965162329746	16.871467374915195
95-99	0.36025566000344084	47.82206042506597	34.93080742936759	16.886876485562997
100-104	0.37249654870050086	47.792733518751966	34.91695019499517	16.917819737552367
105-109	0.3756366421596266	47.759980916729155	34.93452324137347	16.929859199737745
110-114	0.39407827719592886	47.74254553050394	34.907521538515454	16.955854653784673
115-119	0.40576015907553786	47.7216217178355	34.895010028137364	16.9776080949516
120-124	0.4250085897332269	47.68762338621932	34.887545720313156	16.999822303734298
125-129	0.42758654577445	47.68227368194681	34.87767303252129	17.01246673975745
130-134	0.454734717721749	47.63089563764836	34.86147249706598	17.052897147563915
135-139	0.47534529128975284	47.60505607211427	34.84472889390462	17.074869742691348
140-144	0.504941789282082	47.568651685151025	34.821377143444785	17.1050293821221
145-149	0.5233547350697217	47.506647866178476	34.8039151412632	17.166082257488608
150	0.5448926254507368	47.47961686179926	34.7789667292076	17.1965237835424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5283.0
1	6500.5
2	11294.5
3	24881.5
4	59582.5
5	139767.0
6	303769.5
7	598813.5
8	1071475.5
9	1742701.5
10	2598260.5
11	3570457.5
12	4553881.5
13	5432089.0
14	6091353.0
15	6469316.5
16	6544827.5
17	6330774.0
18	5884436.5
19	5280436.0
20	4590508.0
21	3884505.0
22	3218855.0
23	2623518.5
24	2111711.5
25	1685067.0
26	1336638.5
27	1054838.5
28	829459.0
29	655079.0
30	520307.0
31	413013.5
32	325123.5
33	253942.0
34	199616.0
35	158319.5
36	126793.0
37	102627.5
38	83924.0
39	68985.5
40	57193.5
41	47882.5
42	40167.5
43	34086.0
44	29263.0
45	25346.0
46	22229.5
47	19508.5
48	17376.5
49	16425.0
50	15887.5
51	14949.0
52	13634.5
53	12066.0
54	10853.5
55	9895.0
56	9051.5
57	8265.0
58	7539.0
59	6899.0
60	6330.0
61	5700.5
62	5156.0
63	4683.0
64	4181.5
65	3800.5
66	3438.5
67	3137.0
68	2866.5
69	2580.5
70	2336.5
71	2090.0
72	1891.0
73	1769.5
74	1611.5
75	1449.0
76	1333.5
77	1253.0
78	1155.0
79	1041.5
80	984.5
81	907.5
82	843.5
83	784.5
84	705.0
85	651.5
86	603.5
87	564.0
88	557.0
89	527.5
90	496.5
91	501.0
92	469.5
93	454.5
94	471.5
95	496.5
96	493.5
97	532.0
98	706.5
99	1201.0
100	2638.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0442748154580694
2	5.193124950785013E-4
3	0.0013971102113459442
4	3.8917744903046075E-4
5	0.008399848915666917
6	0.0010607233942028962
7	8.27462462607352E-4
8	0.0
9	3.069222784151899E-5
10-14	3.737085661983352E-4
15-19	3.5897629683440607E-4
20-24	0.0013141184272624769
25-29	0.0015262631061030564
30-34	0.001970195489602787
35-39	6.322598935352911E-4
40-44	0.002119728023646667
45-49	0.002050486357636201
50-54	5.109642091056081E-4
55-59	6.182642376395585E-4
60-64	0.0013008593848349407
65-69	3.0004721937868965E-4
70-74	7.940693187157792E-4
75-79	0.0013774671855273722
80-84	0.001005722921910894
85-89	0.002030843331817628
90-94	0.0010511474191163424
95-99	7.805647384655109E-4
100-104	0.0011090943452811302
105-109	0.0014027575812687837
110-114	9.075077928180334E-4
115-119	0.0029818113192592527
120-124	0.00168758145563808
125-129	5.416564369471271E-4
130-134	0.001975351783880162
135-139	8.836906240130148E-4
140-144	0.001873944663091783
145-149	7.881764109702075E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.1453846E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.12351968156378
#Duplication Level	Percentage of total
1	75.34544529784688
2	15.54793257681827
3	3.769135612937518
4	1.2332235893609715
5	0.663115656338197
6	0.4881579439743565
7	0.378157561098013
8	0.26398980520919485
9	0.2551320527412053
>10	1.7342967147463102
>50	0.11414234177632425
>100	0.1501464366158572
>500	0.02403223081098681
>1k	0.02438683929778442
>5k	0.008705340428135627
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.087071493223291E-5	0.0	0.0	0.0	0.17171073788216212	1.2276891136607595E-6
2	2.3326093159554432E-5	0.0	0.0	0.0	0.22079865940277393	1.2276891136607595E-6
3	2.3326093159554432E-5	0.0	0.0	1.2276891136607595E-6	0.25615856125443115	1.2276891136607595E-6
4	2.455378227321519E-5	0.0	0.0	1.2276891136607595E-6	0.29550968041459946	6.138445568303797E-6
5	2.455378227321519E-5	0.0	0.0	1.2276891136607595E-6	0.3364997645415049	7.366134681964557E-6
6	2.455378227321519E-5	0.0	0.0	1.2276891136607595E-6	0.377212390928723	7.366134681964557E-6
7	2.455378227321519E-5	0.0	0.0	1.2276891136607595E-6	0.41786363286025807	7.366134681964557E-6
8	3.0692227841518984E-5	0.0	0.0	1.2276891136607595E-6	0.4565726706139818	8.593823795625317E-6
9	3.3147606068840506E-5	0.0	0.0	1.2276891136607595E-6	0.4973626414153606	1.2276891136607595E-5
10-11	3.437529518250127E-5	0.0	0.0	1.2276891136607595E-6	0.5580509973709529	1.6573803034420253E-5
12-13	3.989989619397468E-5	0.0	0.0	1.2276891136607595E-6	0.6369410721256796	2.3326093159554432E-5
14-15	4.726603087593924E-5	0.0	0.0	1.2276891136607595E-6	0.713954010225619	3.560298429616202E-5
16-17	6.568136758085063E-5	0.0	0.0	2.455378227321519E-6	0.7910350850713667	4.4810652648617725E-5
18-19	9.08489944108962E-5	0.0	0.0	2.455378227321519E-6	0.8675219583861025	5.033525366009114E-5
20-21	1.387288698436658E-4	0.0	0.0	2.455378227321519E-6	0.9446681007548741	5.7087543785225315E-5
22-23	2.0747946020866837E-4	0.0	0.0	2.455378227321519E-6	1.0211555879141667	6.199830023986835E-5
24-25	2.786854288009924E-4	0.0	0.0	2.455378227321519E-6	1.0975448599443665	6.506752302402026E-5
26-27	3.756728687801924E-4	0.0	0.0	2.455378227321519E-6	1.174181511330969	6.997827947866329E-5
28-29	5.051940702714026E-4	0.0	0.0	3.0692227841518987E-6	1.2515707361442454	7.427519137647595E-5
30-31	6.242799142964962E-4	0.0	0.0	3.6830673409822783E-6	1.3287972921499618	7.795825871745822E-5
32-33	7.844933436292254E-4	0.0	0.0	3.6830673409822783E-6	1.4061417063105899	8.16413260584405E-5
34-35	9.250637471433823E-4	0.0	0.0	3.6830673409822783E-6	1.4823818141134797	8.593823795625317E-5
36-37	0.0010736141298963342	0.0	0.0	3.6830673409822783E-6	1.5593370508250772	8.777977162674431E-5
38-39	0.0012227783572061166	0.0	0.0	3.6830673409822783E-6	1.6366372681776131	8.839361618357468E-5
40-41	0.001411228636153043	0.0	0.0	3.6830673409822783E-6	1.7137705198106914	9.453206175187848E-5
42-43	0.0015714420654857721	0.0	0.0	3.6830673409822783E-6	1.7905851861187747	1.0067050732018228E-4
44-45	0.0017678723236714937	0.0	0.0	3.6830673409822783E-6	1.8671788683863988	1.0373973010433417E-4
46-47	0.001961233359073063	0.0	0.0	3.6830673409822783E-6	1.9436381186960774	1.1171970934312911E-4
48-49	0.0021846727777593217	0.0	0.0	3.6830673409822783E-6	2.020217068694338	1.1663046579777215E-4
50-51	0.0024136367974570533	0.0	0.0	3.6830673409822783E-6	2.096875204640429	1.2890735693437975E-4
52-53	0.002637690060700142	0.0	0.0	3.6830673409822783E-6	2.1730275326716924	1.3565964705951393E-4
54-55	0.0028844555725459544	0.0	0.0	3.6830673409822783E-6	2.2494781891575752	1.4179809262781772E-4
56-57	0.0031250826388234633	0.0	0.0	3.6830673409822783E-6	2.3260141700368573	1.5223345009393417E-4
58-59	0.003371848150669276	0.0	0.0	3.6830673409822783E-6	2.401630611770008	1.6021342933272912E-4
60-61	0.0036327320873221873	0.0	0.0	4.910756454643038E-6	2.4768652421888095	1.7064878679884555E-4
62-63	0.003894229868531929	0.0	0.0	4.910756454643038E-6	2.5522416707002393	1.8353952249228355E-4
64-65	0.004210973659856405	0.0	0.0	4.910756454643038E-6	2.627774015729104	1.933610354015696E-4
66-67	0.004489045244100567	0.0	0.0	4.910756454643038E-6	2.7033965959078223	2.0932099387915948E-4
68-69	0.004829115128584598	0.0	0.0	6.138445568303797E-6	2.7783820054365513	2.2650864147041012E-4
70-71	0.005164274256613985	6.138445568303798E-7	0.0	6.752290125134177E-6	2.852911328459555	2.436962890616608E-4
72-73	0.0055381055917236854	1.2276891136607595E-6	0.0	7.366134681964557E-6	2.9276929415954163	2.633393148802329E-4
74-75	0.005939559931890754	1.8415336704911394E-6	0.0	7.979979238794936E-6	3.0028655737139776	2.8850694171027845E-4
76-77	0.006380914168251798	2.455378227321519E-6	0.0	9.821512909286076E-6	3.078736147093656	3.1735763588130637E-4
78-79	0.006842525274988243	2.455378227321519E-6	0.0	1.0435357466116455E-5	3.1533342207070247	3.455944854955038E-4
80-81	0.007305364070838349	2.455378227321519E-6	0.0	1.1663046579777216E-5	3.2287757167414783	3.738313351097013E-4
82-83	0.007801350472757296	2.455378227321519E-6	0.0	1.2276891136607595E-5	3.3037488493790703	3.867220708031392E-4
84-85	0.008341533682768031	2.455378227321519E-6	0.0	1.2276891136607595E-5	3.378218015635505	4.1066200851952407E-4
86-87	0.008989753534780912	2.455378227321519E-6	0.0	1.2276891136607595E-5	3.4524895239446396	4.4135423636104307E-4
88-89	0.009614647293634239	2.455378227321519E-6	0.0	1.2890735693437974E-5	3.52636878558196	4.714326196457317E-4
90-91	0.010319340844875513	2.455378227321519E-6	0.0	1.3504580250268354E-5	3.600136941354494	4.978279355894379E-4
92-93	0.011092171141924963	2.455378227321519E-6	0.0	1.3504580250268354E-5	3.674318214513775	5.162432722943494E-4
94-95	0.01193006896199843	2.455378227321519E-6	0.0	1.3504580250268354E-5	3.748419687880668	5.493908783631899E-4
96-97	0.012806025144595383	2.455378227321519E-6	0.0	1.3504580250268354E-5	3.8214549132523468	5.764000388637266E-4
98-99	0.013889460787401001	2.455378227321519E-6	0.0	1.3504580250268354E-5	3.894689024260438	6.015676656937721E-4
100-101	0.014990697922354704	2.455378227321519E-6	0.0	1.3504580250268354E-5	3.9680397657343276	6.451506292287292E-4
102-103	0.01619567478741274	2.455378227321519E-6	0.0	1.3504580250268354E-5	4.0414482085965595	6.801397689680608E-4
104-105	0.01757314197294011	2.455378227321519E-6	6.138445568303798E-7	1.4732269363929113E-5	4.114987400349396	7.280196444008303E-4
106-107	0.018984984453649982	2.455378227321519E-6	1.2276891136607595E-6	1.5346113920759492E-5	4.1891207936332435	7.691472297084658E-4
108-109	0.0205613372755904	2.455378227321519E-6	1.2276891136607595E-6	1.5959958477589873E-5	4.261894521223713	8.243932398232E-4
110-111	0.022318774241795777	2.455378227321519E-6	1.2276891136607595E-6	1.5959958477589873E-5	4.334443581706381	8.820946281652556E-4
112-113	0.024252998440368302	2.455378227321519E-6	1.2276891136607595E-6	1.5959958477589873E-5	4.407433382580854	9.367267937231595E-4
114-115	0.026338842244477936	2.455378227321519E-6	1.2276891136607595E-6	1.6573803034420253E-5	4.480253148513086	9.870620473832507E-4
116-117	0.028684956140683643	2.455378227321519E-6	1.2276891136607595E-6	1.7187647591250634E-5	4.552599026447444	0.0010429219020548152
118-119	0.03128827090620129	2.455378227321519E-6	1.2276891136607595E-6	1.7187647591250634E-5	4.625180620691625	0.0011067617359651746
120-121	0.034145103473689875	2.455378227321519E-6	1.2276891136607595E-6	1.7187647591250634E-5	4.697701444324679	0.0011693738807618734
122-123	0.037320521366173434	2.455378227321519E-6	1.2276891136607595E-6	1.780149214808101E-5	4.770325393843281	0.0012454906058088405
124-125	0.0408458306560503	2.455378227321519E-6	1.2276891136607595E-6	1.9643025818572152E-5	4.842176513064834	0.00132529039819679
126-127	0.044778118887105714	2.455378227321519E-6	1.2276891136607595E-6	2.0256870375402533E-5	4.91462551688474	0.0014081594133688912
128-129	0.049054773914542965	2.455378227321519E-6	1.2276891136607595E-6	2.087071493223291E-5	4.98646742843794	0.0014867315166431799
130-131	0.05377585240112542	2.455378227321519E-6	1.2276891136607595E-6	2.087071493223291E-5	5.058491651824519	0.0015683728427016202
132-133	0.058999669579751945	2.455378227321519E-6	1.2276891136607595E-6	2.087071493223291E-5	5.130014364208168	0.0016401926558507747
134-135	0.06464397027980728	2.455378227321519E-6	1.2276891136607595E-6	2.209840404589367E-5	5.201639588632807	0.0017470016087392608
136-137	0.07117527636448254	2.455378227321519E-6	1.2276891136607595E-6	2.209840404589367E-5	5.273280773015924	0.0018482859606162735
138-139	0.07825106257106632	2.455378227321519E-6	1.2276891136607595E-6	2.209840404589367E-5	5.345094447719509	0.0019557087580615896
>>END_MODULE
