SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR10065375_1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.2
Python version: could not detect
Number of cores used for trimming: 2
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Illumina adapter for trimming (count: 576). Second best hit was Nextera (count: 0)
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 4.2 with Python 3.10.8
Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065375_1.fastq.gz
Processing single-end reads on 2 cores ...
Finished in 1533.836 s (18.831 µs/read; 3.19 M reads/minute).

=== Summary ===

Total reads processed:              81,453,846
Reads with adapters:                33,346,208 (40.9%)
Reads written (passing filters):    81,453,846 (100.0%)

Total basepairs processed: 12,218,076,900 bp
Quality-trimmed:              22,415,408 bp (0.2%)
Total written (filtered):  12,151,104,751 bp (99.5%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 33346208 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 41.3%
  C: 0.1%
  G: 12.7%
  T: 45.9%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	26731399	20363461.5	0	26731399
2	4321640	5090865.4	0	4321640
3	1578055	1272716.3	0	1578055
4	624808	318179.1	0	624808
5	5691	79544.8	0	5691
6	5667	19886.2	0	5667
7	4223	4971.5	0	4223
8	3788	1242.9	0	3788
9	4406	310.7	0	3501 905
10	4930	77.7	1	3419 1511
11	3591	19.4	1	3140 451
12	3945	4.9	1	3693 252
13	2682	1.2	1	2565 117
14	2753	1.2	1	2630 123
15	2553	1.2	1	2456 97
16	2448	1.2	1	2328 120
17	2180	1.2	1	2083 97
18	2246	1.2	1	2135 111
19	1981	1.2	1	1906 75
20	1907	1.2	1	1831 76
21	1793	1.2	1	1702 91
22	1779	1.2	1	1692 87
23	1674	1.2	1	1598 76
24	1849	1.2	1	1760 89
25	1534	1.2	1	1468 66
26	1346	1.2	1	1278 68
27	1287	1.2	1	1234 53
28	1269	1.2	1	1204 65
29	1201	1.2	1	1132 69
30	1249	1.2	1	1193 56
31	993	1.2	1	949 44
32	1010	1.2	1	975 35
33	972	1.2	1	934 38
34	881	1.2	1	831 50
35	893	1.2	1	857 36
36	874	1.2	1	826 48
37	721	1.2	1	699 22
38	698	1.2	1	662 36
39	757	1.2	1	712 45
40	749	1.2	1	692 57
41	780	1.2	1	733 47
42	625	1.2	1	590 35
43	637	1.2	1	603 34
44	471	1.2	1	446 25
45	655	1.2	1	630 25
46	287	1.2	1	262 25
47	417	1.2	1	397 20
48	454	1.2	1	433 21
49	388	1.2	1	363 25
50	388	1.2	1	366 22
51	343	1.2	1	326 17
52	385	1.2	1	359 26
53	372	1.2	1	355 17
54	304	1.2	1	289 15
55	313	1.2	1	292 21
56	232	1.2	1	217 15
57	278	1.2	1	258 20
58	250	1.2	1	228 22
59	172	1.2	1	164 8
60	214	1.2	1	209 5
61	173	1.2	1	158 15
62	170	1.2	1	161 9
63	210	1.2	1	200 10
64	189	1.2	1	179 10
65	133	1.2	1	126 7
66	202	1.2	1	183 19
67	151	1.2	1	141 10
68	135	1.2	1	124 11
69	148	1.2	1	128 20
70	168	1.2	1	154 14
71	260	1.2	1	219 41
72	839	1.2	1	798 41
73	199	1.2	1	178 21
74	57	1.2	1	53 4
75	50	1.2	1	41 9
76	36	1.2	1	30 6
77	23	1.2	1	20 3
78	30	1.2	1	25 5
79	19	1.2	1	17 2
80	30	1.2	1	25 5
81	22	1.2	1	19 3
82	24	1.2	1	16 8
83	31	1.2	1	27 4
84	23	1.2	1	20 3
85	13	1.2	1	10 3
86	13	1.2	1	8 5
87	16	1.2	1	12 4
88	18	1.2	1	9 9
89	21	1.2	1	16 5
90	16	1.2	1	10 6
91	14	1.2	1	7 7
92	16	1.2	1	10 6
93	7	1.2	1	6 1
94	10	1.2	1	5 5
95	16	1.2	1	11 5
96	9	1.2	1	7 2
97	9	1.2	1	7 2
98	8	1.2	1	5 3
99	12	1.2	1	8 4
100	15	1.2	1	11 4
101	7	1.2	1	5 2
102	12	1.2	1	11 1
103	6	1.2	1	4 2
104	9	1.2	1	5 4
105	7	1.2	1	4 3
106	13	1.2	1	7 6
107	6	1.2	1	5 1
108	8	1.2	1	5 3
109	8	1.2	1	5 3
110	8	1.2	1	6 2
111	9	1.2	1	5 4
112	9	1.2	1	7 2
113	11	1.2	1	6 5
114	9	1.2	1	8 1
115	8	1.2	1	6 2
116	4	1.2	1	3 1
117	12	1.2	1	10 2
118	2	1.2	1	2
119	13	1.2	1	12 1
120	9	1.2	1	7 2
121	5	1.2	1	4 1
122	6	1.2	1	2 4
123	4	1.2	1	3 1
124	7	1.2	1	6 1
125	7	1.2	1	2 5
126	7	1.2	1	4 3
127	2	1.2	1	2
128	1	1.2	1	0 1
129	1	1.2	1	1
130	5	1.2	1	4 1
131	1	1.2	1	1
132	3	1.2	1	3
133	4	1.2	1	0 4
134	1	1.2	1	1
135	3	1.2	1	2 1
136	1	1.2	1	1
137	3	1.2	1	0 3
138	3	1.2	1	0 3
139	1	1.2	1	0 1
141	3	1.2	1	0 3
142	2	1.2	1	1 1
143	2	1.2	1	1 1
146	2	1.2	1	0 2
147	3	1.2	1	1 2
148	3	1.2	1	0 3
149	3	1.2	1	0 3
150	33	1.2	1	5 28

RUN STATISTICS FOR INPUT FILE: SRR10065375_1.fastq.gz
=============================================
81453846 sequences processed in total