##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065374_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76534362
Total Bases	11.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.76533203477936	32.0	32.0	32.0	32.0	32.0
2	30.94982648447504	32.0	32.0	32.0	32.0	32.0
3	34.5750186563259	37.0	32.0	37.0	32.0	37.0
4	35.302321315489635	37.0	37.0	37.0	32.0	37.0
5	35.590806074792916	37.0	37.0	37.0	32.0	37.0
6	39.0869169850792	41.0	41.0	41.0	37.0	41.0
7	38.947293034206	41.0	41.0	41.0	37.0	41.0
8	39.27422946310051	41.0	41.0	41.0	37.0	41.0
9	39.30063279288851	41.0	41.0	41.0	37.0	41.0
10-14	39.34971277607305	41.0	41.0	41.0	37.0	41.0
15-19	39.382766851835775	41.0	41.0	41.0	37.0	41.0
20-24	39.33546017147174	41.0	41.0	41.0	37.0	41.0
25-29	39.29360209731676	41.0	41.0	41.0	37.0	41.0
30-34	39.230846554910855	41.0	41.0	41.0	37.0	41.0
35-39	39.17303553402588	41.0	41.0	41.0	37.0	41.0
40-44	39.06419437585434	41.0	41.0	41.0	37.0	41.0
45-49	38.930478380416886	41.0	41.0	41.0	37.0	41.0
50-54	38.78089444059127	41.0	41.0	41.0	36.0	41.0
55-59	38.687055464054176	41.0	41.0	41.0	32.0	41.0
60-64	38.63870033959387	41.0	41.0	41.0	32.0	41.0
65-69	38.51807685023884	41.0	41.0	41.0	32.0	41.0
70-74	38.383252928926225	41.0	41.0	41.0	32.0	41.0
75-79	38.15328391187215	41.0	39.4	41.0	31.0	41.0
80-84	38.306386088382105	41.0	41.0	41.0	32.0	41.0
85-89	38.18069119854948	41.0	41.0	41.0	32.0	41.0
90-94	38.02020243926513	41.0	39.4	41.0	32.0	41.0
95-99	37.80975149959439	41.0	37.0	41.0	27.0	41.0
100-104	37.561237126403434	41.0	37.0	41.0	27.0	41.0
105-109	37.354291971493794	41.0	37.0	41.0	27.0	41.0
110-114	37.09926773545195	41.0	37.0	41.0	27.0	41.0
115-119	36.78095221072071	41.0	37.0	41.0	25.0	41.0
120-124	36.43832433071043	41.0	37.0	41.0	22.0	41.0
125-129	36.09930988384015	41.0	37.0	41.0	22.0	41.0
130-134	35.67789432150751	41.0	32.0	41.0	22.0	41.0
135-139	35.19483078986142	41.0	32.0	41.0	22.0	41.0
140-144	34.65088626204266	37.0	32.0	41.0	18.0	41.0
145-149	34.05021150891674	37.0	32.0	41.0	12.0	41.0
150	33.65583483925821	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	78.0
12	633.0
13	2767.0
14	6957.0
15	14653.0
16	24129.0
17	35962.0
18	53759.0
19	82422.0
20	131526.0
21	207222.0
22	301417.0
23	398049.0
24	495144.0
25	600295.0
26	717638.0
27	848183.0
28	991200.0
29	1143212.0
30	1291054.0
31	1432953.0
32	1568405.0
33	1733598.0
34	1993828.0
35	2465289.0
36	3292995.0
37	4748285.0
38	7629622.0
39	1.485798E7
40	2.9465107E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.17331391844245989	51.10079753712829	32.08908850319409	16.636800041235166
2	0.16821309518170877	52.47303236638678	34.79030690658222	12.568447631849294
3	0.20673215456236066	48.11051535468946	36.05104123982376	15.631711250924418
4	0.24248682815103456	46.91519673059524	36.150908718359545	16.691407722894176
5	0.2828219382545834	47.549583904673625	35.79607090017235	16.37152325689944
6	0.27315323656938223	47.26990752479715	35.93897153508973	16.517967703543743
7	0.28243243741169083	47.84015805220032	35.59821920021587	16.279190310172112
8	0.25543951094803663	46.38116144484225	36.85134528200549	16.512053762204225
9	0.27794058413750283	46.71085110118473	36.75428689575345	16.25692141892431
10-14	0.26590199590423935	47.25786868133667	36.428150359288516	16.048078963470573
15-19	0.27914357799062384	46.97420761161193	36.6062723603786	16.140376450018856
20-24	0.29360989205530436	47.09086776511825	36.31154166855789	16.303980674268562
25-29	0.2988375570798722	47.09617301869004	36.18638027232732	16.418609151902768
30-34	0.30045758786732146	47.1537721793275	36.085066503983995	16.460703728821187
35-39	0.30176272983062613	47.15596519721922	36.0805635484761	16.46170852447406
40-44	0.31238578571268455	47.162728229509334	36.02093064677854	16.503955337999447
45-49	0.3157498946386232	47.13105632967605	36.0424712499174	16.510722525767935
50-54	0.3184678738149943	47.12558048954607	36.02541734675495	16.530534289883985
55-59	0.3229828142174058	47.1238768927875	36.01111907397782	16.54202121901728
60-64	0.32203643779918256	47.113297191431386	35.99758625415566	16.567080116613774
65-69	0.3317748877367417	47.08346588640852	35.99833824710669	16.586420978748055
70-74	0.34212501901841463	47.05417920970018	35.9918209018219	16.611874869459502
75-79	0.3417712266782061	47.04026049229693	35.99666325385771	16.62130502716715
80-84	0.3602763800267075	47.011333723527216	35.97930862679869	16.64908126964739
85-89	0.35917237126149704	46.99801561369655	35.98354440014735	16.659267614894606
90-94	0.3637229129815881	46.96479363682646	35.978962800858085	16.69252064933387
95-99	0.3754847661510475	46.931831678239284	35.9695874542284	16.72309610138127
100-104	0.38877463157580544	46.895854938362696	35.960200462094164	16.755169967967333
105-109	0.3927112985361201	46.856437223411795	35.97442452949195	16.77642694856014
110-114	0.41318110637612515	46.817827635315425	35.960907153546295	16.808084104762152
115-119	0.42437900230056136	46.794175989194464	35.95052337744157	16.8309216310634
120-124	0.44519831261268317	46.75124662565015	35.945294758540584	16.85826030319658
125-129	0.4506814061263216	46.732505907922004	35.94044857368742	16.876364112264255
130-134	0.47914712160962286	46.68502465716082	35.921865550903156	16.913962670326402
135-139	0.5014632253088928	46.63942959064761	35.90835395475884	16.95075322928466
140-144	0.5342933494371098	46.60431148426937	35.886755394720346	16.974639771573177
145-149	0.5572254547520514	46.52624064025161	35.872893345079994	17.043640559916344
150	0.5815923571689277	46.51036223441701	35.84287277393127	17.065172634482796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5150.0
1	6790.5
2	13357.5
3	31122.0
4	73338.0
5	166364.0
6	347549.5
7	660650.5
8	1148450.0
9	1825003.0
10	2663983.0
11	3595165.5
12	4512625.0
13	5294981.0
14	5849589.0
15	6121846.0
16	6108020.5
17	5843092.0
18	5376861.0
19	4776090.0
20	4114980.0
21	3458291.5
22	2847792.5
23	2311820.5
24	1859059.0
25	1486095.0
26	1181340.5
27	934639.0
28	737587.0
29	583763.5
30	465460.0
31	372030.0
32	295713.5
33	234270.0
34	186368.5
35	149875.0
36	122269.0
37	100491.5
38	83323.5
39	69759.5
40	58716.5
41	49404.5
42	41727.0
43	35884.5
44	31216.0
45	27060.5
46	23911.5
47	21166.5
48	18668.0
49	17713.5
50	17035.0
51	15970.0
52	14741.5
53	13155.5
54	11766.0
55	10668.0
56	9685.0
57	8681.0
58	7957.5
59	7278.0
60	6663.0
61	6063.0
62	5356.5
63	4889.0
64	4477.0
65	3982.5
66	3655.0
67	3280.5
68	2967.0
69	2739.5
70	2452.5
71	2237.0
72	2058.5
73	1915.5
74	1724.0
75	1537.0
76	1423.0
77	1303.5
78	1217.0
79	1097.5
80	984.5
81	916.5
82	840.5
83	784.5
84	741.0
85	680.0
86	638.0
87	607.5
88	569.5
89	553.0
90	513.0
91	476.5
92	475.5
93	474.5
94	494.0
95	506.0
96	542.5
97	636.0
98	762.5
99	1282.5
100	2960.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0363789274156359
2	6.363154892439033E-4
3	0.0013497205346795732
4	4.0243361537396755E-4
5	0.008762077352915022
6	9.982444225510104E-4
7	8.623577472299305E-4
8	0.0
9	2.4825450299043455E-5
10-14	3.911968326070321E-4
15-19	3.5408931742319876E-4
20-24	0.0013606959969170449
25-29	0.0015817731648432637
30-34	0.0020430038993465446
35-39	6.841371461357449E-4
40-44	0.0022034547044372044
45-49	0.0020555472847607983
50-54	5.560900866985734E-4
55-59	6.619249011313376E-4
60-64	0.0013523337399742093
65-69	3.1253935323848395E-4
70-74	8.404068227549869E-4
75-79	0.0014633949649962457
80-84	0.0010259443986741536
85-89	0.002067306708586661
90-94	0.0010816056714499037
95-99	7.899719605685091E-4
100-104	0.0011372669442256539
105-109	0.0014262874498124124
110-114	9.24552033242271E-4
115-119	0.0030671190543144528
120-124	0.0017281126613428882
125-129	5.621004588762365E-4
130-134	0.002070442554940224
135-139	9.067822372387451E-4
140-144	0.0018600795287220138
145-149	7.847455499792367E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.6534362E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.73999665819947
#Duplication Level	Percentage of total
1	74.5765176608293
2	16.178827993736764
3	3.930398121636478
4	1.3294520306830286
5	0.673230639372265
6	0.48323853884632945
7	0.3310917122130121
8	0.3002101179044589
9	0.2160291141402602
>10	1.6477320878447888
>50	0.141744592009982
>100	0.1301476284920343
>500	0.024895883247949117
>1k	0.028761678598256384
>5k	0.007722200445159166
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.4825450299043455E-5	0.0	0.0	0.0	0.15663552536049102	1.0452821178544613E-5
2	2.4825450299043455E-5	0.0	0.0	0.0	0.2014337037264386	4.703769530345076E-5
3	2.6132052946361532E-5	0.0	0.0	2.6132052946361533E-6	0.23395243041289088	6.010372177663152E-5
4	2.7438655593679608E-5	0.0	0.0	2.6132052946361533E-6	0.26978731461823646	8.492917207567497E-5
5	2.7438655593679608E-5	0.0	0.0	2.6132052946361533E-6	0.30698367878208743	1.0844801972740035E-4
6	3.005186088831576E-5	0.0	0.0	2.6132052946361533E-6	0.34456418412425	1.2020744355326304E-4
7	3.3971668830269994E-5	0.0	0.0	2.6132052946361533E-6	0.3820323216387431	1.3327347002644381E-4
8	4.181128471417845E-5	0.0	0.0	2.6132052946361533E-6	0.418098474512664	1.3719327796839803E-4
9	4.3117887361496525E-5	0.0	0.0	2.6132052946361533E-6	0.45666415825090434	1.607121256201234E-4
10-11	4.899759927442787E-5	0.0	0.0	3.919807941954229E-6	0.5127076123009949	1.692050428276909E-4
12-13	5.4224009863700176E-5	0.0	0.0	3.919807941954229E-6	0.5864000016097345	1.8357767194818978E-4
14-15	7.186314560249421E-5	0.0	0.0	3.919807941954229E-6	0.6584532578974135	2.084031222472332E-4
16-17	9.407539060690152E-5	0.0	0.0	5.2264105892723065E-6	0.7305842570426079	2.1885594342577783E-4
18-19	1.19554142229604E-4	0.0	0.0	6.533013236590383E-6	0.8017542499406998	2.3322857254627665E-4
20-21	1.8553757591916684E-4	0.0	0.0	6.533013236590383E-6	0.8735167871393505	2.4302809240116225E-4
22-23	2.4694790034311643E-4	0.0	0.0	6.533013236590383E-6	0.9458196045326673	2.5413421490336587E-4
24-25	3.194643472692697E-4	0.0	0.0	7.18631456024942E-6	1.0175886747445546	2.6001392681629723E-4
26-27	3.959006021373772E-4	0.0	0.0	7.839615883908458E-6	1.089284575208192	2.658936387292286E-4
28-29	4.971623073045282E-4	0.0	0.0	9.146218531226537E-6	1.1618460999256779	2.7046674799484187E-4
30-31	6.036504230609514E-4	0.0	0.0	9.146218531226537E-6	1.234138464497816	2.815728704970455E-4
32-33	7.591361380918024E-4	0.0	0.0	9.146218531226537E-6	1.3057311433523155	2.881058837336359E-4
34-35	8.930629094419053E-4	0.0	0.0	9.146218531226537E-6	1.3777347487393963	2.9071908902827205E-4
36-37	0.001041362309912507	0.0	0.0	1.0452821178544613E-5	1.4494809534049555	2.992120062358395E-4
38-39	0.0011896617103831087	0.0	0.0	1.0452821178544613E-5	1.5211474553090283	3.083582247670661E-4
40-41	0.0013405743161483464	0.0	0.0	1.0452821178544613E-5	1.5924115235977272	3.155445393273155E-4
42-43	0.0015123925642706736	0.0	0.0	1.0452821178544613E-5	1.6641845136175566	3.24690757858542E-4
44-45	0.0016940103322478862	0.0	0.0	1.0452821178544613E-5	1.7357889518958816	3.4232989359733604E-4
46-47	0.0018815078121380301	0.0	0.0	1.0452821178544613E-5	1.807239864363147	3.534360160995397E-4
48-49	0.0020716184973228104	0.0	0.0	1.0452821178544613E-5	1.878613033972897	3.658487412490614E-4
50-51	0.002236250430884888	0.0	0.0	1.0452821178544613E-5	1.9499228333542522	3.847944796351735E-4
52-53	0.002438120539895531	0.0	0.0	1.0452821178544613E-5	2.021501139579631	3.959006021373772E-4
54-55	0.002632804334345924	0.0	0.0	1.175942382586269E-5	2.0923118167497106	4.1353973787617123E-4
56-57	0.002850353675124384	0.0	0.0	1.175942382586269E-5	2.1630493241715403	4.3313877758594237E-4
58-59	0.003086195452965297	0.0	0.0	1.175942382586269E-5	2.2339423173084008	4.468581053827822E-4
60-61	0.0033213839294825506	0.0	0.0	1.175942382586269E-5	2.305015621610591	4.6188403582694007E-4
62-63	0.003551999296734191	0.0	0.0	1.175942382586269E-5	2.3755408061022316	4.749500623001208E-4
64-65	0.0037649755282470375	0.0	0.0	1.3066026473180766E-5	2.4457648186836654	5.004288139228233E-4
66-67	0.004005390415353563	0.0	0.0	1.3066026473180766E-5	2.515327690325556	5.226410589272306E-4
68-69	0.004265404342169861	0.0	0.0	1.3066026473180766E-5	2.584978104344817	5.409334959896837E-4
70-71	0.004556123431198133	0.0	0.0	1.3066026473180766E-5	2.65467359615541	5.651056449650681E-4
72-73	0.004876241079791062	0.0	0.0	1.3066026473180766E-5	2.724747349432403	5.918909992350886E-4
74-75	0.005235556807803533	0.0	0.0	1.3066026473180766E-5	2.7951666991096102	6.343555852729261E-4
76-77	0.005622311191409683	0.0	0.0	1.3066026473180766E-5	2.8647099717117914	6.840064858710132E-4
78-79	0.006057409872966603	0.0	0.0	1.3066026473180766E-5	2.9335881835664877	7.284309758798276E-4
80-81	0.006510147690262317	0.0	0.0	1.3066026473180766E-5	3.00301386193041	7.6762905529937E-4
82-83	0.006928260537404101	0.0	0.0	1.4372629120498842E-5	3.0719482576989403	8.068271347189122E-4
84-85	0.00741823653014838	0.0	0.0	1.4372629120498842E-5	3.1410864834804526	8.54518131346022E-4
86-87	0.00794087758907561	0.0	0.0	1.4372629120498842E-5	3.209917004338522	9.015558266494729E-4
88-89	0.008568700161111945	0.0	0.0	1.5679231767816917E-5	3.2791447324013756	9.414072073926742E-4
90-91	0.009254666550953937	0.0	0.0	1.5679231767816917E-5	3.34767277474659	9.812585881358755E-4
92-93	0.00995827207653472	0.0	0.0	1.5679231767816917E-5	3.415865673512768	0.0010184967635844407
94-95	0.010771632224490223	0.0	0.0	1.6985834415134997E-5	3.483717548987996	0.001075333978742777
96-97	0.011667961640550423	0.0	0.0	1.7639135738794037E-5	3.5515328395890986	0.0011210650713989097
98-99	0.012706057443844634	0.0	0.0	1.8292437062453073E-5	3.6193945145841813	0.0011759423825862688
100-101	0.013862400786721133	0.0	0.0	1.8292437062453073E-5	3.6865859024211893	0.0012262465845080148
102-103	0.015082114357992558	0.0	0.0	1.894573838611211E-5	3.7536361771722877	0.0012713243758404884
104-105	0.016438367905908722	0.0	0.0	1.959903970977115E-5	3.8214266423230914	0.0013275082896751658
106-107	0.01798865194695162	0.0	0.0	2.025234103343019E-5	3.888477570375513	0.00138565210748082
108-109	0.019617332146833602	0.0	0.0	2.0905642357089226E-5	3.9549842984253267	0.0014594751570542915
110-111	0.021519092300005063	0.0	0.0	2.0905642357089226E-5	4.0219215520474325	0.0015306850013331267
112-113	0.023628602274100095	0.0	0.0	2.0905642357089226E-5	4.088162386458517	0.0015960151336990305
114-115	0.025939329055882115	0.0	0.0	2.0905642357089226E-5	4.154607050882583	0.0016665716766542066
116-117	0.028453232549322097	0.0	0.0	2.0905642357089226E-5	4.22115559021711	0.0017345150143147467
118-119	0.03130358622444648	0.0	0.0	2.0905642357089226E-5	4.287188021505948	0.0017959253387386962
120-121	0.03446556463095622	0.0	0.0	2.2212245004407302E-5	4.353098938748585	0.0018743214975777808
122-123	0.037999271490627956	0.0	0.0	2.2865546328066342E-5	4.419528577242207	0.0019553308617115015
124-125	0.042061499121139864	0.0	0.0	2.351884765172538E-5	4.485676642865331	0.0020376468284925404
126-127	0.04640791282744344	0.0	0.0	2.351884765172538E-5	4.551573187478848	0.002152627861456531
128-129	0.05121490396692664	0.0	0.0	2.351884765172538E-5	4.617915283595099	0.0022617291825075903
130-131	0.056513831003125105	0.0	0.0	2.351884765172538E-5	4.683825547536412	0.0023616842850274233
132-133	0.062352384932665934	0.0	0.0	2.351884765172538E-5	4.749531981464743	0.002474052112696778
134-135	0.0689827400664815	0.0	0.0	2.6132052946361532E-5	4.814764118632098	0.002591646350955405
136-137	0.07642449544428162	0.0	0.0	2.6132052946361532E-5	4.879953137912093	0.002695521261417192
138-139	0.08471488924151481	0.0	0.0	2.6785354270020568E-5	4.945363626340806	0.002811808897028501
>>END_MODULE
