##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065374_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76534362
Total Bases	11.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178163071379625	32.0	32.0	32.0	32.0	32.0
2	31.457137043881023	32.0	32.0	32.0	32.0	32.0
3	35.0258437380062	37.0	32.0	37.0	32.0	37.0
4	35.835645484312	37.0	37.0	37.0	32.0	37.0
5	36.17440391023316	37.0	37.0	37.0	37.0	37.0
6	39.629027142082926	41.0	41.0	41.0	37.0	41.0
7	39.845192163488605	41.0	41.0	41.0	37.0	41.0
8	39.93752101833684	41.0	41.0	41.0	37.0	41.0
9	39.97546235506608	41.0	41.0	41.0	37.0	41.0
10-14	40.000841723878224	41.0	41.0	41.0	37.0	41.0
15-19	39.98912962258704	41.0	41.0	41.0	37.0	41.0
20-24	39.9462145173432	41.0	41.0	41.0	37.0	41.0
25-29	39.818391247581054	41.0	41.0	41.0	37.0	41.0
30-34	39.72285746107089	41.0	41.0	41.0	37.0	41.0
35-39	39.670809370567426	41.0	41.0	41.0	37.0	41.0
40-44	39.610948303194846	41.0	41.0	41.0	37.0	41.0
45-49	39.553390209223934	41.0	41.0	41.0	37.0	41.0
50-54	39.456457223750036	41.0	41.0	41.0	37.0	41.0
55-59	39.376990486965845	41.0	41.0	41.0	37.0	41.0
60-64	39.296287283351234	41.0	41.0	41.0	37.0	41.0
65-69	39.19145071334103	41.0	41.0	41.0	37.0	41.0
70-74	39.10093984189742	41.0	41.0	41.0	37.0	41.0
75-79	38.919339919499166	41.0	40.2	41.0	36.0	41.0
80-84	39.15429844440331	41.0	41.0	41.0	37.0	41.0
85-89	39.06131054179298	41.0	41.0	41.0	37.0	41.0
90-94	38.98103147969013	41.0	41.0	41.0	37.0	41.0
95-99	38.86440925972572	41.0	41.0	41.0	36.0	41.0
100-104	38.705279557958555	41.0	41.0	41.0	32.0	41.0
105-109	38.57967992468533	41.0	41.0	41.0	32.0	41.0
110-114	38.438858780844086	41.0	41.0	41.0	32.0	41.0
115-119	38.2411727793589	41.0	37.0	41.0	32.0	41.0
120-124	38.094072764335586	41.0	37.0	41.0	32.0	41.0
125-129	37.82760139556662	41.0	37.0	41.0	32.0	41.0
130-134	37.59993685189406	41.0	37.0	41.0	27.0	41.0
135-139	37.37794720232985	41.0	37.0	41.0	27.0	41.0
140-144	37.08444407232401	41.0	37.0	41.0	27.0	41.0
145-149	36.78806215435624	41.0	37.0	41.0	27.0	41.0
150	36.60152100046251	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	10.0
14	21.0
15	89.0
16	437.0
17	1366.0
18	2832.0
19	5062.0
20	8542.0
21	14054.0
22	23892.0
23	40012.0
24	67482.0
25	110629.0
26	173260.0
27	260432.0
28	377809.0
29	528551.0
30	718063.0
31	947722.0
32	1226557.0
33	1562321.0
34	1980454.0
35	2524761.0
36	3295156.0
37	4541430.0
38	7073429.0
39	1.4605012E7
40	3.6444972E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.802152316082696	40.647566781768326	43.113792406423705	0.43648849572527043
2	17.225386039008946	41.14174120604266	41.370927827812594	0.26194492713579975
3	15.39631855401489	37.12175816113803	47.17164173711827	0.3102815477288042
4	15.780865605805463	36.68069908239324	47.20621949075877	0.33221582104252956
5	16.181356551975963	36.79617267861983	46.695132834582196	0.32733793482200846
6	16.297875456255845	36.51655187247788	46.84638515703573	0.3391875142305361
7	16.748540369101562	36.57005384240246	46.36197539667869	0.319430391817297
8	15.966261120592339	35.97731238123374	47.725518462288555	0.3309080358853692
9	16.333957288361532	35.812469436930826	47.532966956724614	0.32060631798302575
10-14	16.15233751423883	36.61848196562209	46.92398401086291	0.30519650927616804
15-19	16.122880475024537	36.32301678495069	47.248071122350765	0.30603161767401144
20-24	16.26218702147695	36.27892116541982	47.14874127926523	0.31015053383799646
25-29	16.396636634770186	36.12393377404317	47.16640122479799	0.3130283663886615
30-34	16.493671430879637	36.04178159870203	47.14870243512319	0.31584453529513973
35-39	16.532740662524027	35.990340848789565	47.159848121293344	0.317070367393061
40-44	16.54571739974094	35.975702053582594	47.155412850314164	0.32316769636229264
45-49	16.555336334342776	35.96751363261864	47.15281903640653	0.3243309966320555
50-54	16.573277852516437	35.94464131152017	47.158708822249224	0.3233720137141666
55-59	16.577993779026883	35.96000617682012	47.13260735522845	0.329392688924543
60-64	16.571453779248923	35.95155309140861	47.15197318352021	0.3250199458222478
65-69	16.58435991858526	35.953067054530514	47.13295448922904	0.3296185376551792
70-74	16.585084035112203	35.954519533068	47.1266856502451	0.3337107815746868
75-79	16.58759749296644	35.952372337321364	47.133861923075834	0.32616824663636496
80-84	16.5980159428228	35.93456615327779	47.13503249418118	0.33238540971822306
85-89	16.619992772049326	35.926848002640476	47.104197732596845	0.34896149271334603
90-94	16.592853348290646	35.93191053322718	47.13870180415706	0.33653431432511927
95-99	16.60369662730067	35.92678077715421	47.12597489193808	0.3435477036070428
100-104	16.591195462216447	35.92648089323553	47.12497566188959	0.3573479826584342
105-109	16.58180077418775	35.93914334954669	47.12890582029948	0.35015005596607396
110-114	16.598722439435935	35.91777221951368	47.121148701625955	0.36235663942442936
115-119	16.577092291812846	35.911634063901175	47.14074083466211	0.3705328096238685
120-124	16.56349944862125	35.92077961819704	47.14985816220258	0.3658627709791347
125-129	16.582264855243455	35.91091346304684	47.10962571639541	0.3971959653142877
130-134	16.557636045288522	35.92307517119791	47.12200216248609	0.397286621027481
135-139	16.562355421763552	35.902065927494206	47.12097109584168	0.4146075549005611
140-144	16.554372076616495	35.90598333448381	47.116916185301946	0.42272840359775965
145-149	16.558753719745503	35.883802625483376	47.11567802670504	0.4417656280660774
150	16.547303458825304	35.90147263852998	47.10872364080097	0.4425002618437522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5582.0
1	7845.5
2	15528.5
3	34028.5
4	76307.0
5	166041.5
6	337757.5
7	635848.5
8	1101716.5
9	1750107.5
10	2560867.5
11	3466877.0
12	4366519.0
13	5155386.0
14	5742688.5
15	6066783.0
16	6111876.5
17	5898785.5
18	5469746.5
19	4889679.5
20	4238565.0
21	3578656.0
22	2953720.0
23	2397230.0
24	1926138.0
25	1539743.0
26	1225295.0
27	970597.0
28	768460.5
29	610912.0
30	488205.0
31	391252.5
32	309919.5
33	243119.5
34	191958.0
35	152483.0
36	121405.0
37	97491.5
38	79090.0
39	64531.0
40	52152.5
41	42330.5
42	34383.0
43	27942.0
44	22884.0
45	18654.0
46	15151.5
47	12658.5
48	10944.5
49	10459.0
50	10773.5
51	10290.0
52	8855.0
53	7338.0
54	6260.5
55	5423.0
56	4801.5
57	4118.0
58	3447.0
59	2989.0
60	2536.5
61	2128.0
62	1796.5
63	1457.0
64	1172.0
65	962.5
66	780.0
67	623.5
68	501.0
69	409.5
70	341.0
71	277.5
72	230.0
73	184.0
74	150.5
75	128.5
76	113.5
77	109.5
78	97.0
79	83.0
80	71.0
81	63.5
82	51.5
83	47.0
84	44.0
85	37.0
86	36.0
87	29.0
88	30.5
89	33.5
90	30.0
91	28.0
92	35.0
93	51.5
94	52.0
95	52.0
96	60.0
97	63.5
98	75.0
99	141.0
100	770.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7461707200224652
2	0.001435956309402566
3	0.00884439331969606
4	4.95202403333551E-4
5	0.0
6	0.0
7	8.231596678103883E-4
8	1.398064832630342E-4
9	0.0
10-14	5.226410589272306E-7
15-19	3.185497254161471E-4
20-24	3.927647557838138E-4
25-29	6.10706077356469E-4
30-34	0.0
35-39	2.4015356657706245E-4
40-44	3.778694856043877E-4
45-49	2.655016579350332E-4
50-54	9.323916491261793E-4
55-59	6.318730402430218E-4
60-64	3.786534471927786E-4
65-69	1.7769796003525842E-5
70-74	5.55044804580719E-4
75-79	0.0022379490143264015
80-84	1.7795928056472203E-4
85-89	4.688090298577258E-4
90-94	1.5156590708889688E-4
95-99	2.445960155779439E-4
100-104	0.0027783598692571582
105-109	4.1471568025875754E-4
110-114	4.1184115443465775E-4
115-119	8.892737617646828E-4
120-124	4.912825953915968E-5
125-129	3.940713584311319E-4
130-134	0.0015065128523577423
135-139	1.4085176538088866E-4
140-144	5.213344562799125E-4
145-149	3.8597042201775984E-4
150	2.2212245004407302E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.6534362E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.95418847821139
#Duplication Level	Percentage of total
1	73.43695904065906
2	16.65332340010364
3	4.104650859252311
4	1.4242782394585272
5	0.7382074378332987
6	0.4941727101934461
7	0.3821885274746996
8	0.28710414049940447
9	0.24813672655309418
>10	1.8589306566713877
>50	0.1531883922019014
>100	0.14149393317049944
>500	0.03385470592041197
>1k	0.022878547360189877
>5k	0.02063268264818851
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.351884765172538E-5	0.0	0.0	0.0	0.003888449478418596	0.0028797522346890405
2	2.351884765172538E-5	0.0	0.0	0.0	0.013431875214429827	0.006365768097733669
3	2.4825450299043455E-5	0.0	0.0	0.0	0.021123844999191344	0.008799968829687246
4	2.7438655593679608E-5	0.0	0.0	0.0	0.029546205663803664	0.011641829587604062
5	2.7438655593679608E-5	0.0	0.0	0.0	0.0379986181893043	0.01487044472912703
6	3.005186088831576E-5	0.0	0.0	0.0	0.04659737021130456	0.018545917976032777
7	3.6584874124906146E-5	0.0	0.0	0.0	0.05487208477676994	0.02222008462029121
8	4.4424490008814605E-5	0.0	0.0	0.0	0.06282014868040581	0.02577927023158565
9	4.573109265613268E-5	0.0	0.0	0.0	0.07115235376235318	0.029491328352616305
10-11	5.226410589272306E-5	0.0	0.0	0.0	0.08306007698868648	0.03531485635171297
12-13	5.5530612511018256E-5	0.0	0.0	0.0	0.09759537813877642	0.043266186761967126
14-15	6.598343368956287E-5	0.0	0.0	0.0	0.1118092550376261	0.051808754870132714
16-17	8.362256942835689E-5	0.0	0.0	0.0	0.12570170768523556	0.060636162355413636
18-19	9.668859590153766E-5	0.0	0.0	0.0	0.1392021795386496	0.0694008529136233
20-21	1.2739375811351246E-4	0.0	0.0	0.0	0.15246550301157535	0.07818252930624808
22-23	1.502593044415788E-4	0.0	0.0	0.0	0.1651924660977771	0.08678324123221932
24-25	1.6855174150403186E-4	0.0	0.0	0.0	0.17743467960182382	0.09544209697599622
26-27	1.9011068518478012E-4	0.0	0.0	0.0	0.18976966189383013	0.10396506604445203
28-29	2.286554632806634E-4	0.0	0.0	0.0	0.20204715366935444	0.11260628265249013
30-31	2.5217431093238875E-4	0.0	0.0	0.0	0.21435927041503267	0.12115081066462668
32-33	3.201176485929288E-4	0.0	0.0	0.0	0.22661193674025792	0.1296084496007166
34-35	3.658487412490615E-4	0.0	0.0	0.0	0.2390573269559626	0.13798834567929108
36-37	4.057001219922628E-4	0.0	0.0	0.0	0.2517183066084748	0.14639764031743024
38-39	4.43591598764487E-4	0.0	0.0	0.0	0.2643609938239245	0.1548814113064665
40-41	4.8213637686037026E-4	0.0	0.0	0.0	0.27694488392024486	0.16349714916288188
42-43	5.389735920187065E-4	0.0	0.0	0.0	0.2894549509669918	0.17205670310546262
44-45	5.98424012471679E-4	0.0	0.0	0.0	0.30197089772565167	0.18059665800833358
46-47	6.611409395429466E-4	0.0	0.0	0.0	0.31451166993461055	0.1891052544476689
48-49	7.264710719088506E-4	1.3066026473180766E-6	0.0	0.0	0.32718179580565393	0.19771053948290573
50-51	7.918012042747544E-4	1.3066026473180766E-6	0.0	0.0	0.34006163140159185	0.20640598010080752
52-53	8.564780353169993E-4	1.3066026473180766E-6	0.0	0.0	0.35269647900115764	0.21515368482460207
54-55	9.348741941560838E-4	1.3066026473180766E-6	0.0	6.533013236590383E-7	0.3654665599747209	0.22408823372696307
56-57	0.0010093505450532142	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.3782033225807775	0.23281895261634244
58-59	0.0011073457436020697	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.3911824599779116	0.2417175699459022
60-61	0.0011850886011174956	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.40406621538179155	0.2504750741895516
62-63	0.0013000696340814862	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.41690489299433897	0.25952983053546586
64-65	0.0013889186140991154	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.42988533699412035	0.2685937330999114
66-67	0.0015097793589760374	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.442846181954192	0.2778013619555618
68-69	0.0016332533091475959	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.4559899512848882	0.28711547892696876
70-71	0.0017717531897633119	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.4692558879631087	0.2963433601236527
72-73	0.0019213591928812315	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.4827321876675473	0.30592663724040714
74-75	0.0021232293018918743	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.4962182868918408	0.3154596101552398
76-77	0.002359071079732787	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5095377942786012	0.3251559084009873
78-79	0.002647176963466423	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5228932332381631	0.3347992892395183
80-81	0.002926789929992492	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5365851223794091	0.3441918023697643
82-83	0.0031815774462195163	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5503802331297933	0.35352943818882293
84-85	0.0035056149027543997	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5639055304335066	0.36316301950749913
86-87	0.0038819164651820058	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5776653629124131	0.37278810790896777
88-89	0.00434118729571431	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.5911272899877313	0.38215906209553296
90-91	0.004871667970525448	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6048440045792765	0.3918253084803921
92-93	0.005445919834021743	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6184490046444759	0.40159477647438935
94-95	0.006092688144444191	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6322284361630923	0.411104883842894
96-97	0.006834838448120858	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6459823889300861	0.42059539217168884
98-99	0.007697196195350789	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6595096461377701	0.4300983132256332
100-101	0.008673228372897392	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6733413417622793	0.43966721771326717
102-103	0.00973484302384333	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.6873128438700515	0.4492818532935572
104-105	0.010921891528931802	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.7016501947190728	0.458860557301046
106-107	0.012306237033765304	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.7157615033101079	0.468452327335008
108-109	0.013780738121263753	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.7301269461160466	0.4782100359051794
110-111	0.015521786148815091	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.744534853508023	0.48785537664768147
112-113	0.01749018303699977	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.7588774307676336	0.49757454043975696
114-115	0.019650650514340215	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.7731193996234005	0.5072322939074085
116-117	0.0220240942231935	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.7875083612769909	0.5168521558982879
118-119	0.024724188593876303	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8018561649471907	0.5265785060049236
120-121	0.027726108176089587	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8164790084746509	0.5363597334227468
122-123	0.031120661853821945	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8312103000218386	0.5459149185825838
124-125	0.03499016559385443	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8458076647976761	0.5555151815337533
126-127	0.03917652047586155	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8605049846760335	0.5651036850610971
128-129	0.043873103691646376	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8753218586966205	0.5749567756245227
130-131	0.0490753421319433	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.8904353315181487	0.5846217154067346
132-133	0.05481786076690624	1.3066026473180766E-6	0.0	1.3066026473180766E-6	0.9056206674852794	0.5942520302187924
134-135	0.06135544711276224	1.959903970977115E-6	0.0	1.3066026473180766E-6	0.9206002135354574	0.6039600878883657
136-137	0.06872795255025449	2.6132052946361533E-6	0.0	1.3066026473180766E-6	0.9356575024431509	0.6137713671670773
138-139	0.07690401861584735	2.6132052946361533E-6	0.0	1.959903970977115E-6	0.9510244561782588	0.6236799883430139
>>END_MODULE
