SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR10065374_1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.2
Python version: could not detect
Number of cores used for trimming: 2
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Illumina adapter for trimming (count: 665). Second best hit was smallRNA (count: 1)
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 4.2 with Python 3.10.8
Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065374_1.fastq.gz
Processing single-end reads on 2 cores ...
Finished in 1368.654 s (17.883 µs/read; 3.36 M reads/minute).

=== Summary ===

Total reads processed:              76,534,362
Reads with adapters:                32,348,544 (42.3%)
Reads written (passing filters):    76,534,362 (100.0%)

Total basepairs processed: 11,480,154,300 bp
Quality-trimmed:              22,137,176 bp (0.2%)
Total written (filtered):  11,414,551,888 bp (99.4%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 32348544 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 42.4%
  C: 0.2%
  G: 12.7%
  T: 44.7%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	25911075	19133590.5	0	25911075
2	4151906	4783397.6	0	4151906
3	1589700	1195849.4	0	1589700
4	596913	298962.4	0	596913
5	6193	74740.6	0	6193
6	6207	18685.1	0	6207
7	4734	4671.3	0	4734
8	4308	1167.8	0	4308
9	4923	292.0	0	3920 1003
10	5357	73.0	1	3758 1599
11	4022	18.2	1	3551 471
12	4591	4.6	1	4258 333
13	3033	1.1	1	2876 157
14	3083	1.1	1	2916 167
15	2683	1.1	1	2573 110
16	2899	1.1	1	2767 132
17	2422	1.1	1	2303 119
18	2620	1.1	1	2495 125
19	2091	1.1	1	2004 87
20	2037	1.1	1	1957 80
21	1998	1.1	1	1921 77
22	1912	1.1	1	1835 77
23	1838	1.1	1	1758 80
24	2059	1.1	1	1941 118
25	1624	1.1	1	1542 82
26	1509	1.1	1	1444 65
27	1485	1.1	1	1432 53
28	1383	1.1	1	1325 58
29	1347	1.1	1	1280 67
30	1314	1.1	1	1245 69
31	1159	1.1	1	1106 53
32	1096	1.1	1	1049 47
33	1076	1.1	1	1020 56
34	941	1.1	1	902 39
35	949	1.1	1	902 47
36	886	1.1	1	844 42
37	829	1.1	1	796 33
38	817	1.1	1	772 45
39	761	1.1	1	728 33
40	756	1.1	1	705 51
41	680	1.1	1	637 43
42	647	1.1	1	609 38
43	681	1.1	1	651 30
44	485	1.1	1	466 19
45	841	1.1	1	807 34
46	218	1.1	1	195 23
47	407	1.1	1	387 20
48	456	1.1	1	432 24
49	402	1.1	1	385 17
50	408	1.1	1	386 22
51	352	1.1	1	335 17
52	387	1.1	1	353 34
53	329	1.1	1	308 21
54	308	1.1	1	284 24
55	387	1.1	1	364 23
56	225	1.1	1	205 20
57	303	1.1	1	276 27
58	248	1.1	1	232 16
59	191	1.1	1	183 8
60	190	1.1	1	179 11
61	212	1.1	1	205 7
62	177	1.1	1	160 17
63	248	1.1	1	232 16
64	163	1.1	1	149 14
65	114	1.1	1	100 14
66	178	1.1	1	158 20
67	128	1.1	1	121 7
68	119	1.1	1	107 12
69	125	1.1	1	109 16
70	179	1.1	1	159 20
71	291	1.1	1	224 67
72	841	1.1	1	797 44
73	210	1.1	1	195 15
74	69	1.1	1	55 14
75	33	1.1	1	25 8
76	26	1.1	1	20 6
77	33	1.1	1	26 7
78	23	1.1	1	17 6
79	26	1.1	1	21 5
80	34	1.1	1	24 10
81	22	1.1	1	19 3
82	16	1.1	1	9 7
83	16	1.1	1	15 1
84	30	1.1	1	25 5
85	18	1.1	1	17 1
86	21	1.1	1	14 7
87	13	1.1	1	11 2
88	17	1.1	1	10 7
89	22	1.1	1	17 5
90	14	1.1	1	12 2
91	13	1.1	1	10 3
92	10	1.1	1	9 1
93	10	1.1	1	10
94	13	1.1	1	10 3
95	12	1.1	1	7 5
96	21	1.1	1	13 8
97	11	1.1	1	7 4
98	14	1.1	1	12 2
99	12	1.1	1	7 5
100	11	1.1	1	9 2
101	9	1.1	1	6 3
102	13	1.1	1	5 8
103	14	1.1	1	6 8
104	7	1.1	1	5 2
105	9	1.1	1	7 2
106	10	1.1	1	8 2
107	4	1.1	1	3 1
108	13	1.1	1	10 3
109	8	1.1	1	6 2
110	6	1.1	1	3 3
111	8	1.1	1	4 4
112	5	1.1	1	4 1
113	9	1.1	1	6 3
114	4	1.1	1	4
115	11	1.1	1	4 7
116	9	1.1	1	7 2
117	13	1.1	1	6 7
118	3	1.1	1	1 2
119	14	1.1	1	13 1
120	8	1.1	1	2 6
121	7	1.1	1	2 5
122	3	1.1	1	2 1
123	6	1.1	1	5 1
124	5	1.1	1	1 4
125	6	1.1	1	1 5
126	3	1.1	1	2 1
127	3	1.1	1	3
128	1	1.1	1	1
129	5	1.1	1	3 2
130	4	1.1	1	3 1
131	9	1.1	1	4 5
132	1	1.1	1	1
133	1	1.1	1	1
134	7	1.1	1	2 5
135	3	1.1	1	1 2
136	1	1.1	1	0 1
137	3	1.1	1	1 2
138	2	1.1	1	0 2
140	2	1.1	1	0 2
141	2	1.1	1	0 2
142	1	1.1	1	1
143	3	1.1	1	1 2
144	4	1.1	1	1 3
145	1	1.1	1	1
146	1	1.1	1	1
147	2	1.1	1	1 1
148	3	1.1	1	2 1
149	4	1.1	1	0 4
150	48	1.1	1	5 43

RUN STATISTICS FOR INPUT FILE: SRR10065374_1.fastq.gz
=============================================
76534362 sequences processed in total