##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065373_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84868974
Total Bases	12.7 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19910207704408	32.0	32.0	32.0	32.0	32.0
2	31.43237554633334	32.0	32.0	32.0	32.0	32.0
3	34.99347733366024	37.0	32.0	37.0	32.0	37.0
4	35.780893297944196	37.0	37.0	37.0	32.0	37.0
5	36.15878265477794	37.0	37.0	37.0	37.0	37.0
6	39.6416330189169	41.0	41.0	41.0	37.0	41.0
7	39.84088682396467	41.0	41.0	41.0	37.0	41.0
8	39.92602036169307	41.0	41.0	41.0	37.0	41.0
9	39.957693868197346	41.0	41.0	41.0	37.0	41.0
10-14	39.98424099011731	41.0	41.0	41.0	37.0	41.0
15-19	39.968841353024956	41.0	41.0	41.0	37.0	41.0
20-24	39.92759682943734	41.0	41.0	41.0	37.0	41.0
25-29	39.79775985980459	41.0	41.0	41.0	37.0	41.0
30-34	39.698926175306426	41.0	41.0	41.0	37.0	41.0
35-39	39.65037338379983	41.0	41.0	41.0	37.0	41.0
40-44	39.57286854911195	41.0	41.0	41.0	37.0	41.0
45-49	39.52486909527149	41.0	41.0	41.0	37.0	41.0
50-54	39.44630221404586	41.0	41.0	41.0	37.0	41.0
55-59	39.36832175913897	41.0	41.0	41.0	37.0	41.0
60-64	39.270815223947444	41.0	41.0	41.0	37.0	41.0
65-69	39.15523854453572	41.0	41.0	41.0	37.0	41.0
70-74	39.07388197010606	41.0	41.0	41.0	37.0	41.0
75-79	38.9003995900787	41.0	40.2	41.0	36.0	41.0
80-84	39.13552248669814	41.0	41.0	41.0	37.0	41.0
85-89	39.04581716281854	41.0	41.0	41.0	37.0	41.0
90-94	38.939399969652044	41.0	41.0	41.0	37.0	41.0
95-99	38.81288353503602	41.0	41.0	41.0	35.0	41.0
100-104	38.678894520393285	41.0	41.0	41.0	32.0	41.0
105-109	38.55100842623595	41.0	41.0	41.0	32.0	41.0
110-114	38.400810984235534	41.0	40.2	41.0	32.0	41.0
115-119	38.24658406969784	41.0	37.0	41.0	32.0	41.0
120-124	38.00645050805021	41.0	37.0	41.0	32.0	41.0
125-129	37.82788245089425	41.0	37.0	41.0	32.0	41.0
130-134	37.59360533096583	41.0	37.0	41.0	27.0	41.0
135-139	37.31682659908201	41.0	37.0	41.0	27.0	41.0
140-144	37.067072518161936	41.0	37.0	41.0	27.0	41.0
145-149	36.80657522736165	41.0	37.0	41.0	27.0	41.0
150	36.639069620424536	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	13.0
14	20.0
15	101.0
16	434.0
17	1257.0
18	3032.0
19	5345.0
20	9354.0
21	15776.0
22	26548.0
23	45378.0
24	75465.0
25	121461.0
26	191435.0
27	286568.0
28	414244.0
29	579713.0
30	786738.0
31	1037686.0
32	1347026.0
33	1725339.0
34	2200561.0
35	2824811.0
36	3724410.0
37	5207454.0
38	8200689.0
39	1.6823124E7
40	3.9214983E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.008378412865303	41.7376658954602	42.8635203934718	0.39043529820269807
2	17.21475331490732	41.06242608894826	41.46510055096228	0.2577200451821338
3	15.139567717384558	37.71116868716561	46.83538877664102	0.3138748188088148
4	15.882971975881944	37.01798543454072	46.772967436143915	0.32607515343342364
5	16.30052226152752	36.99760880813759	46.38254022017516	0.31932871015973396
6	16.389172044529097	36.51235566681944	46.764068331640686	0.33440395701078385
7	16.778090239343108	36.686766577542414	46.22196874634948	0.31317443676499274
8	16.03903429935212	36.16275092362991	47.47865856712025	0.31955620989773054
9	16.36433185406222	36.02152305067877	47.29442462876592	0.31972046649309055
10-14	16.26723352838562	36.636471426958614	46.79247348716537	0.30382155749038964
15-19	16.247682464561468	36.257608383904675	47.189172056845024	0.3055370946888284
20-24	16.435651751261148	36.20500264017271	47.04950625702126	0.3098393515448896
25-29	16.576062249913004	36.0088819167835	47.09613951321443	0.31891632008906284
30-34	16.687720643068275	35.94259847568986	47.048245721063175	0.32143516017868873
35-39	16.730476526430454	35.85707425019926	47.089245675571505	0.3232035477987768
40-44	16.729247052256575	35.87456689202816	47.07006885084337	0.3261172048718866
45-49	16.73856886172722	35.85348936707217	47.08192949589816	0.3260122753024492
50-54	16.7438196934169	35.85203574991321	47.07676795464996	0.3273766020199307
55-59	16.746396031301565	35.854493924269946	47.06931013260938	0.3297999118191127
60-64	16.73012951254411	35.862825329173255	47.07473692269593	0.33230823558671274
65-69	16.749654344479993	35.85237215357626	47.062936822662714	0.33503667928102937
70-74	16.739288943868768	35.86739945865736	47.05492660976646	0.33838498770741204
75-79	16.745672707026856	35.857007170842685	47.06527481276816	0.33204530936229504
80-84	16.746013446545692	35.847562114415	47.073996855583346	0.3324275834559635
85-89	16.760964741716638	35.84324086284838	47.04774702623764	0.34804736919734136
90-94	16.742860225678328	35.83575626525038	47.07574250098504	0.34564100808625725
95-99	16.75288210236368	35.8468905487266	47.04780738750266	0.35241996140706033
100-104	16.72643529463794	35.84442623618329	47.07579497660917	0.35334349256960623
105-109	16.73134274782963	35.84934480452001	47.057969435789595	0.3613430118607669
110-114	16.73542347721769	35.84078308951171	47.06329414540371	0.36049928786688523
115-119	16.718890359048203	35.840761676012285	47.071926484897496	0.3684214800420119
120-124	16.708405366646335	35.83415958004503	47.075996752113106	0.3814383011955206
125-129	16.71572548189056	35.84523714611402	47.05063317816645	0.3884041938289656
130-134	16.707264918496907	35.849818358497046	47.04039046980385	0.40252625320220126
135-139	16.714491914330388	35.82915091449724	47.03804541395053	0.4183117572218438
140-144	16.704091165302884	35.828858816660386	47.04096048183436	0.4260895362023716
145-149	16.69820938661621	35.80764045968657	47.05663797573523	0.43751217796199104
150	16.69273127902545	35.8318372650015	47.034814071273125	0.44061738469992706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5616.0
1	7981.5
2	15623.5
3	34133.0
4	76659.0
5	168716.0
6	347729.5
7	660856.0
8	1155858.0
9	1851836.5
10	2730431.0
11	3725828.0
12	4726547.5
13	5612274.5
14	6284311.5
15	6674383.0
16	6753056.0
17	6543544.0
18	6101226.5
19	5487833.0
20	4777989.5
21	4048252.0
22	3355211.5
23	2732736.5
24	2202161.0
25	1766319.5
26	1411451.5
27	1121716.5
28	889088.5
29	708988.5
30	568804.0
31	456025.0
32	362113.5
33	284853.0
34	225435.5
35	179853.0
36	143916.5
37	116266.5
38	94350.0
39	76935.0
40	62551.0
41	50770.5
42	41632.5
43	34067.0
44	27443.5
45	22282.5
46	18232.0
47	15083.5
48	12788.5
49	11962.0
50	12261.5
51	11756.0
52	9900.0
53	7943.5
54	6784.0
55	5799.0
56	4883.0
57	4259.0
58	3815.0
59	3446.0
60	2962.5
61	2481.5
62	1954.0
63	1410.5
64	1111.5
65	892.0
66	694.0
67	578.5
68	485.5
69	383.0
70	297.5
71	236.0
72	200.5
73	155.5
74	139.0
75	126.0
76	110.0
77	96.0
78	103.0
79	112.0
80	103.0
81	84.0
82	63.5
83	55.0
84	51.5
85	49.5
86	45.0
87	42.0
88	30.5
89	20.5
90	20.5
91	19.0
92	12.0
93	10.0
94	12.5
95	13.0
96	10.5
97	15.0
98	24.5
99	74.0
100	993.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7702932758442443
2	0.0010781325104743224
3	8.778237380364702E-4
4	0.008541401714129359
5	0.0
6	7.305378759498141E-5
7	0.0011028765353048807
8	2.8278885520637966E-5
9	1.767430345039873E-4
10-14	2.476759056849208E-4
15-19	3.0753288003693786E-4
20-24	3.3416216390220533E-4
25-29	0.0014811066291434134
30-34	3.3816833935096233E-4
35-39	9.970663719818269E-4
40-44	6.235494257300671E-4
45-49	0.0011830000442800215
50-54	6.346253225589837E-4
55-59	2.863237158964594E-4
60-64	9.892896784636515E-4
65-69	0.0011226717551693272
70-74	5.109051984061926E-4
75-79	3.716316872170506E-4
80-84	0.0017294895069663503
85-89	0.0010939215548900118
90-94	6.058751222796685E-4
95-99	0.0018072564421481045
100-104	0.0029334630580075114
105-109	2.304729169931994E-4
110-114	3.421745147997194E-4
115-119	5.533235266871496E-4
120-124	3.004631586567784E-4
125-129	0.0014726229634872221
130-134	0.0010248739427437877
135-139	0.0017172353232407405
140-144	0.0012216478544915602
145-149	0.0018034859240786863
150	0.005737078899999427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.4868974E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.73528021409636
#Duplication Level	Percentage of total
1	73.3749005860841
2	16.25822133551167
3	4.120986887506885
4	1.4079236305184808
5	0.7449793861616333
6	0.5611068928966867
7	0.4371497753521505
8	0.32607181560545173
9	0.2489818267110532
>10	2.1011956375449703
>50	0.19625855030377176
>100	0.14109532492337745
>500	0.029727805865778164
>1k	0.028075915130069788
>5k	0.009372267556585318
>10k+	0.013952362327386043
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2961155863625734E-5	0.0	0.0	0.0	0.004040345768761149	0.0013314641932633709
2	1.2961155863625734E-5	0.0	0.0	0.0	0.013748251510616824	0.004852185440582798
3	1.2961155863625734E-5	0.0	0.0	0.0	0.022062243853684386	0.007328944497432007
4	2.0030877243785225E-5	0.0	0.0	0.0	0.030864046971982955	0.010412521306078238
5	2.0030877243785225E-5	0.0	0.0	1.1782868966932485E-6	0.03950913793302132	0.01356561704162937
6	2.0030877243785225E-5	0.0	0.0	1.1782868966932485E-6	0.04816719004992331	0.017273685905523025
7	2.474402483055822E-5	0.0	0.0	1.1782868966932485E-6	0.05678164555164765	0.02095818903148281
8	2.7100598623944717E-5	0.0	0.0	3.5348606900797458E-6	0.06481167075261214	0.024676862477446704
9	3.0635459314024464E-5	0.0	0.0	3.5348606900797458E-6	0.07327177067086967	0.028840928370360647
10-11	3.181374621071771E-5	0.0	0.0	6.480577931812867E-6	0.085107073404705	0.034825447518665655
12-13	3.5937750349144084E-5	0.0	0.0	7.0697213801594915E-6	0.09972607893197813	0.04319246277208441
14-15	4.065089793591708E-5	0.0	0.0	7.0697213801594915E-6	0.11349553960673543	0.051932405828306584
16-17	4.418575862599682E-5	0.0	0.0	8.24800827685274E-6	0.12690856849524304	0.061056470412850755
18-19	5.2433766902849565E-5	0.0	0.0	9.426295173545988E-6	0.139717136205747	0.07041737066363027
20-21	7.24646441466348E-5	0.0	0.0	1.2372012415279111E-5	0.15218341157276155	0.07977061204958127
22-23	9.249552139042002E-5	0.0	0.0	1.2961155863625734E-5	0.16413182984867947	0.08887935890446844
24-25	1.1252639863420523E-4	0.0	0.0	1.2961155863625734E-5	0.17577625010525047	0.09801991950556631
26-27	1.378595669131101E-4	0.0	0.0	1.4139442760318983E-5	0.18736588002112525	0.10728420023081697
28-29	1.6554930898540142E-4	0.0	0.0	1.5317729657012232E-5	0.1988270766652605	0.11629102526914017
30-31	1.9206076416099953E-4	0.0	0.0	1.649601655370548E-5	0.21030123446525933	0.1253161137543621
32-33	2.3388994899360986E-4	0.0	0.0	1.7674303450398727E-5	0.22188909695078912	0.13427462902992088
34-35	2.639362648592877E-4	0.0	0.0	2.062002069213185E-5	0.23363249330668237	0.14324846203513664
36-37	2.9928487176008516E-4	0.0	0.0	2.2387451037171724E-5	0.24539002910533594	0.15221817103621402
38-39	3.346334786608826E-4	0.0	0.0	2.5333168278904845E-5	0.2569867287425909	0.1611454617090104
40-41	3.7469523314845305E-4	0.0	0.0	2.5922311727251468E-5	0.2686252575646785	0.17016348047285218
42-43	4.283072869479959E-4	0.0	0.0	2.651145517559809E-5	0.2802125309067599	0.1790960734366837
44-45	4.618884635037534E-4	0.0	0.0	2.7100598623944717E-5	0.29212854629301865	0.1879726977729223
46-47	5.072525090264435E-4	0.0	0.0	2.7100598623944717E-5	0.30398505819099453	0.19703961544297682
48-49	5.602754193776397E-4	0.0	0.0	2.7100598623944717E-5	0.3162021258793585	0.20648005005928316
50-51	6.221354814540353E-4	0.0	0.0	2.7100598623944717E-5	0.32836027922288774	0.21577850110453792
52-53	6.763366787019247E-4	0.0	0.0	2.7100598623944717E-5	0.3403711467043304	0.22504572754703034
54-55	7.399641711233601E-4	0.0	0.0	2.7689742072291343E-5	0.35254756349475835	0.23456333995507003
56-57	7.859173600943968E-4	0.0	0.0	2.886802896898459E-5	0.36492487820107267	0.24399906142378958
58-59	8.318705490654335E-4	0.0	0.0	3.004631586567784E-5	0.37730337119428353	0.2535602704470069
60-61	8.84304315968283E-4	0.0	0.0	3.0635459314024464E-5	0.3896253064164532	0.26290467468123274
62-63	9.45575234596332E-4	0.0	0.0	3.122460276237109E-5	0.40204445030760005	0.2724393722492745
64-65	0.0010109701573628072	0.0	0.0	3.240288965906433E-5	0.41442765644839774	0.28202944930146084
66-67	0.001091682809786295	0.0	0.0	3.475946345245083E-5	0.42682382374505906	0.29174560305159336
68-69	0.0011688606015197024	0.0	0.0	3.5348606900797454E-5	0.43937434662518715	0.30166795700864724
70-71	0.0012578212622200429	0.0	0.0	3.65268937974907E-5	0.45218703833982954	0.3115549623588003
72-73	0.0013715259477509413	0.0	0.0	3.770518069418395E-5	0.4650026757717137	0.3214955797627529
74-75	0.0015011375063871987	0.0	0.0	3.8883467590877205E-5	0.4778507160932569	0.3314468017487757
76-77	0.001659027950544094	0.0	0.0	4.006175448757045E-5	0.49083484855136816	0.3416048130851682
78-79	0.0017927635133187778	0.0	0.0	4.12400413842637E-5	0.5038236941570662	0.3518712268160565
80-81	0.001927088219541808	0.0	0.0	4.241832828095695E-5	0.516953345046919	0.36187075856484374
82-83	0.0020879243809404364	0.0	0.0	4.241832828095695E-5	0.5301454451422967	0.3720393744833065
84-85	0.002282930862343169	0.0	0.0	4.241832828095695E-5	0.543316925216982	0.38239298144455003
86-87	0.0025050379423698465	0.0	0.0	4.241832828095695E-5	0.5564648395537337	0.3924903110057628
88-89	0.002758958768607242	0.0	0.0	4.241832828095695E-5	0.5699073256146586	0.4026654075021574
90-91	0.003049995632090474	0.0	0.0	4.536404552269007E-5	0.5834588032135277	0.41284934115027716
92-93	0.00338580739764805	0.0	0.0	4.830976276442319E-5	0.596888328118589	0.42287891921493004
94-95	0.003758735200451463	0.0	0.0	4.8898906212769814E-5	0.6101281488332827	0.43311410834305597
96-97	0.004177027048777567	0.0	0.0	5.066633655780969E-5	0.6234816742335072	0.44341587068084504
98-99	0.004660713819870144	0.0	0.0	5.2433766902849565E-5	0.637064965578587	0.4534725493441219
100-101	0.0052180435220060516	0.0	0.0	5.5379484144582687E-5	0.6506618072229788	0.4635580960363678
102-103	0.005770070933106839	0.0	0.0	5.655777104127593E-5	0.6641219675873542	0.474074306589355
104-105	0.006482345362157907	0.0	0.0	5.655777104127593E-5	0.6778920174055598	0.48438254950507587
106-107	0.007321285632603499	0.0	0.0	5.655777104127593E-5	0.6918405776886145	0.49467488554769146
108-109	0.00817141962856768	1.1782868966932485E-6	0.0	5.655777104127593E-5	0.7058339128737434	0.5052989093517262
110-111	0.009203598950070963	1.1782868966932485E-6	0.0	5.655777104127593E-5	0.7199050149940542	0.515753259842637
112-113	0.010336521801241522	1.1782868966932485E-6	0.0	5.773605793796918E-5	0.7341316509847285	0.5261168822425024
114-115	0.01161260651036031	1.1782868966932485E-6	0.0	5.832520138631581E-5	0.7482481171505619	0.5364628303389175
116-117	0.013070736545018207	1.1782868966932485E-6	0.0	5.891434483466243E-5	0.7622402740487944	0.5469048088174131
118-119	0.014836399459713039	1.1782868966932485E-6	0.0	5.891434483466243E-5	0.7764598403180885	0.5574127713621235
120-121	0.016741100228217676	1.1782868966932485E-6	0.0	6.009263173135568E-5	0.7908867850812006	0.5677104096957741
122-123	0.01890266754020144	1.1782868966932485E-6	0.0	6.186006207639555E-5	0.8052512806387879	0.5782331008266932
124-125	0.02146131753637083	1.1782868966932485E-6	0.0	6.244920552474217E-5	0.8195886755977515	0.5885967232265586
126-127	0.02428154722360612	1.1782868966932485E-6	0.0	6.30383489730888E-5	0.8339997134877581	0.5987482539850193
128-129	0.027502983599165462	1.1782868966932485E-6	0.0	6.480577931812867E-5	0.848529758354331	0.6092385422262793
130-131	0.031116200367875307	1.1782868966932485E-6	0.0	6.598406621482193E-5	0.8632500965547197	0.6194124604357771
132-133	0.035116484382148885	1.1782868966932485E-6	0.0	6.598406621482193E-5	0.8777229945068029	0.6299964224853243
134-135	0.03977366334132895	1.1782868966932485E-6	0.0	6.716235311151517E-5	0.8924362629858116	0.6404342769596814
136-137	0.04510187668817582	1.1782868966932485E-6	0.0	7.069721380159491E-5	0.9071512988951651	0.6509039451802492
138-139	0.050972691150949934	1.1782868966932485E-6	0.0	7.187550069828817E-5	0.9219682566210827	0.6615044032463501
>>END_MODULE
