##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065372_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74905667
Total Bases	11.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25191274246313	32.0	32.0	32.0	32.0	32.0
2	31.402286945792767	32.0	32.0	32.0	32.0	32.0
3	35.42705298919506	37.0	37.0	37.0	32.0	37.0
4	35.933328302116315	37.0	37.0	37.0	32.0	37.0
5	36.0764806887041	37.0	37.0	37.0	37.0	37.0
6	39.63341435835556	41.0	41.0	41.0	37.0	41.0
7	39.87024754749197	41.0	41.0	41.0	37.0	41.0
8	39.81529465587697	41.0	41.0	41.0	37.0	41.0
9	39.85677055649207	41.0	41.0	41.0	37.0	41.0
10-14	39.82943593306499	41.0	41.0	41.0	37.0	41.0
15-19	39.823246091647505	41.0	41.0	41.0	37.0	41.0
20-24	39.76703996775037	41.0	41.0	41.0	37.0	41.0
25-29	39.766376808312785	41.0	41.0	41.0	37.0	41.0
30-34	39.70526796857707	41.0	41.0	41.0	37.0	41.0
35-39	39.663158441670376	41.0	41.0	41.0	37.0	41.0
40-44	39.613200995860566	41.0	41.0	41.0	37.0	41.0
45-49	39.55067051735885	41.0	41.0	41.0	37.0	41.0
50-54	39.456923970785816	41.0	41.0	41.0	37.0	41.0
55-59	39.348794485736306	41.0	41.0	41.0	37.0	41.0
60-64	39.268568561040915	41.0	41.0	41.0	37.0	41.0
65-69	39.14235939451684	41.0	41.0	41.0	37.0	41.0
70-74	39.039458512531496	41.0	41.0	41.0	37.0	41.0
75-79	38.64243086440977	41.0	40.2	41.0	33.0	41.0
80-84	38.81778133021631	41.0	41.0	41.0	32.0	41.0
85-89	38.689929289328674	41.0	41.0	41.0	32.0	41.0
90-94	38.49453947990344	41.0	41.0	41.0	32.0	41.0
95-99	38.247398691476846	41.0	41.0	41.0	32.0	41.0
100-104	37.97922039997321	41.0	37.8	41.0	32.0	41.0
105-109	37.657805305972374	41.0	37.0	41.0	29.0	41.0
110-114	37.28359154988901	41.0	37.0	41.0	27.0	41.0
115-119	36.843907890173384	41.0	37.0	41.0	27.0	41.0
120-124	36.42625414976946	41.0	37.0	41.0	22.0	41.0
125-129	35.95278621576122	41.0	34.0	41.0	22.0	41.0
130-134	35.45782311503881	41.0	32.0	41.0	22.0	41.0
135-139	34.8864702025816	41.0	32.0	41.0	22.0	41.0
140-144	34.522249946722994	37.0	32.0	41.0	22.0	41.0
145-149	34.05344146257986	37.0	32.0	41.0	12.0	41.0
150	33.756577082478955	37.0	27.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	195.0
13	1438.0
14	5294.0
15	18252.0
16	48266.0
17	89989.0
18	132777.0
19	175154.0
20	219941.0
21	268515.0
22	315179.0
23	363214.0
24	417995.0
25	474971.0
26	536715.0
27	609692.0
28	688033.0
29	783207.0
30	898876.0
31	1038977.0
32	1222888.0
33	1456942.0
34	1782505.0
35	2244156.0
36	2937411.0
37	4110796.0
38	6420350.0
39	1.2695899E7
40	3.4948035E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.21320977445397563	51.367841663305	32.26150582049059	16.15744274175043
2	0.2283295119323326	52.56387343964807	34.19438519294278	13.013411855476816
3	0.2705656471968355	48.16877404173938	35.59249710162112	15.96816320944266
4	0.33217657580217286	47.12483944634185	35.68770646558631	16.85527751226967
5	0.36335438470203724	47.48445177574031	35.63201365557689	16.520180183980756
6	0.373276786892202	46.89260331655686	35.93624262024594	16.797877276305005
7	0.3551188152282377	47.582869400718245	35.50325733842664	16.55875444562688
8	0.3473126510738475	46.31042052764673	36.617027179074626	16.725239642204798
9	0.3625625062136913	46.699549495613475	36.45937416348379	16.47851383468905
10-14	0.3708719045229012	47.130555551290854	36.160336974226226	16.33823556996002
15-19	0.37464471451679454	46.797381043778195	36.355075488746564	16.472898752958447
20-24	0.395789826717112	47.01858016913283	35.93071076857207	16.654919235577985
25-29	0.3922488070698578	46.992855044384996	35.831929326840594	16.782966821704555
30-34	0.40158090273140257	47.14830961591013	35.64538085600257	16.8047286253559
35-39	0.4069067618032769	47.11368138869388	35.668900414429565	16.810511435073284
40-44	0.41211589568732915	47.18294086988049	35.59084869180087	16.814094542631306
45-49	0.41579677157313133	47.158721571411824	35.61807728513271	16.807404371882342
50-54	0.4195508509803897	47.20543784745843	35.601645775379374	16.77336552618181
55-59	0.42473558341236994	47.205464304870404	35.603036644015766	16.766763467701455
60-64	0.42718731774281815	47.20859552514703	35.59821786904852	16.765999288061632
65-69	0.43732384322519136	47.218512652982824	35.59697993289467	16.74718357089732
70-74	0.4426158351039655	47.21309641055915	35.610212147687726	16.734075606649153
75-79	0.4477587316155378	47.224674794693236	35.60743417364657	16.720132300044664
80-84	0.44589197592719954	47.22375001488543	35.62178357858384	16.70857443060353
85-89	0.45646606840709775	47.236352544570096	35.60768500022869	16.699496386794124
90-94	0.45801180427572546	47.22678607052156	35.62068422164001	16.6945179035627
95-99	0.4810223091595627	47.21661475973152	35.611060993931154	16.691301937177755
100-104	0.48840323508218175	47.22056385780869	35.613749871990265	16.677283035118865
105-109	0.4849190455843074	47.22854164970242	35.6117453299249	16.674793974788376
110-114	0.500270602254123	47.230112015942936	35.61142325331181	16.65819412849113
115-119	0.5216703568778593	47.23658271504623	35.59794591349112	16.643801014584795
120-124	0.5231688411690842	47.24738286814731	35.5977419365786	16.631706354104995
125-129	0.5437868242076437	47.26454689770499	35.575683933564285	16.615982344523086
130-134	0.5586199072060324	47.27078265228734	35.565743774068565	16.604853666438064
135-139	0.5832017339146799	47.27019228791343	35.55295642060803	16.59364955756386
140-144	0.607620245452923	47.27115997589921	35.532095446558756	16.589124332089117
145-149	0.6349934495201219	47.271342017533065	35.51399200348616	16.57967252946066
150	0.6338839489941396	47.27959501381918	35.49274315920583	16.59377787798085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9869.0
1	11924.5
2	18359.0
3	34217.5
4	71278.5
5	151304.5
6	305398.0
7	575897.5
8	1005036.5
9	1611401.5
10	2382447.0
11	3257295.5
12	4141340.0
13	4924033.0
14	5513164.0
15	5854114.5
16	5920582.0
17	5730972.0
18	5333405.0
19	4793200.0
20	4178795.5
21	3550270.0
22	2957039.5
23	2425931.5
24	1970896.0
25	1591792.5
26	1277917.0
27	1020314.0
28	812152.0
29	650291.5
30	524132.5
31	420949.5
32	334749.5
33	264249.0
34	209072.0
35	168350.5
36	137323.5
37	111672.5
38	91136.0
39	74936.0
40	62366.0
41	52024.5
42	43453.0
43	36567.0
44	30814.0
45	25979.5
46	22238.0
47	19430.0
48	17530.5
49	18496.5
50	19021.5
51	17936.5
52	15573.5
53	12116.0
54	10383.5
55	9095.0
56	7983.0
57	7188.0
58	6518.5
59	5896.0
60	5494.5
61	5132.5
62	4709.0
63	4367.5
64	3921.0
65	3467.0
66	3169.5
67	2823.5
68	2420.5
69	2071.5
70	1766.0
71	1517.0
72	1306.5
73	1119.5
74	959.5
75	782.5
76	650.0
77	561.5
78	489.5
79	437.0
80	383.0
81	363.5
82	324.5
83	296.5
84	283.0
85	236.5
86	207.0
87	188.5
88	168.5
89	176.0
90	187.0
91	160.5
92	130.5
93	133.5
94	149.0
95	159.0
96	156.5
97	179.5
98	240.5
99	453.5
100	678.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.158543411675381
2	0.14134444594158677
3	1.335012476425849E-6
4	3.204029943422038E-5
5	9.345087334980943E-6
6	5.246599032353587E-4
7	4.405541172205302E-5
8	3.297480816771847E-4
9	6.861964128828864E-4
10-14	0.0018983877414775574
15-19	0.0011125993978533025
20-24	0.001419919269926533
25-29	1.9918386148273668E-4
30-34	4.1679089514015004E-4
35-39	1.3910830004357347E-4
40-44	1.8156169679391546E-4
45-49	1.1080603554334548E-4
50-54	1.1561208045847853E-4
55-59	6.354659387787042E-4
60-64	0.0011593248345282074
65-69	5.92745539533077E-4
70-74	1.6393953210509427E-4
75-79	5.558991951837235E-4
80-84	2.8702768243155753E-4
85-89	2.2027705861026508E-4
90-94	5.820654397216702E-4
95-99	4.3548106981011194E-4
100-104	0.001612962074017711
105-109	7.833853211666882E-4
110-114	7.022165625999966E-4
115-119	9.879092325551283E-4
120-124	0.0021205338175548185
125-129	4.2907300992326786E-4
130-134	3.7460450088509323E-4
135-139	7.665641639637226E-4
140-144	0.0016692996005228816
145-149	0.0012359545506750512
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.4905667E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.24558628673576
#Duplication Level	Percentage of total
1	75.7066417815113
2	15.011323190994805
3	3.756634271329891
4	1.3312180043717905
5	0.7011641616713806
6	0.49719573440733844
7	0.3701655946213944
8	0.29113308874854693
9	0.2441314040042154
>10	1.6919927099544405
>50	0.1670299737084026
>100	0.1488995811013825
>500	0.04116361679395254
>1k	0.02476730759521129
>5k	0.01653957918600544
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2015112287832642E-5	0.0	0.0	0.0	0.15947258035897338	1.335012476425849E-6
2	1.2015112287832642E-5	0.0	0.0	0.0	0.20318889891201422	1.335012476425849E-6
3	1.2015112287832642E-5	0.0	0.0	0.0	0.2355763015901053	1.335012476425849E-6
4	1.2015112287832642E-5	0.0	0.0	0.0	0.2708273060301299	4.005037429277547E-6
5	1.2015112287832642E-5	0.0	0.0	1.335012476425849E-6	0.30769100554167683	5.340049905703396E-6
6	1.335012476425849E-5	0.0	0.0	1.335012476425849E-6	0.3444292138804398	5.340049905703396E-6
7	1.335012476425849E-5	0.0	0.0	1.335012476425849E-6	0.38100722072203164	5.340049905703396E-6
8	1.7355162193536036E-5	0.0	0.0	1.335012476425849E-6	0.4163676961851231	6.675062382129245E-6
9	1.7355162193536036E-5	0.0	0.0	1.335012476425849E-6	0.4530057785881541	8.010074858555094E-6
10-11	1.8690174669961884E-5	0.0	0.0	2.0025187146387736E-6	0.5071004841329294	9.345087334980943E-6
12-13	2.1360199622813585E-5	0.0	0.0	4.005037429277547E-6	0.577779382166105	1.0012593573193867E-5
14-15	3.00377807195816E-5	0.0	0.0	6.007556143916321E-6	0.6475311140344028	1.0680099811406793E-5
16-17	4.5390424198478865E-5	0.0	0.0	7.34256862034217E-6	0.7168075814610929	1.1347606049619718E-5
18-19	7.142316748878292E-5	0.0	0.0	8.010074858555094E-6	0.7867882679690976	1.2015112287832642E-5
20-21	1.3884129754828832E-4	0.0	0.0	1.2015112287832642E-5	0.8568957806623629	1.2015112287832642E-5
22-23	2.1426950246634878E-4	0.0	6.675062382129245E-7	1.2682618526045565E-5	0.9271134318849334	1.2682618526045565E-5
24-25	2.990427947193902E-4	0.0	1.335012476425849E-6	1.335012476425849E-5	0.9969032116088092	1.335012476425849E-5
26-27	3.924936680691996E-4	0.0	1.335012476425849E-6	1.4017631002471416E-5	1.0668511903111417	1.602014971711019E-5
28-29	4.705918979401118E-4	0.0	1.335012476425849E-6	1.468513724068434E-5	1.1374800253764512	1.602014971711019E-5
30-31	5.907430208184382E-4	0.0	1.335012476425849E-6	1.5352643478897263E-5	1.2078184952281381	1.668765595532311E-5
32-33	7.255792809374489E-4	0.0	1.335012476425849E-6	1.602014971711019E-5	1.278981335284018	1.7355162193536036E-5
34-35	8.951258654435318E-4	0.0	1.335012476425849E-6	1.602014971711019E-5	1.350298369280925	1.9357680908174812E-5
36-37	0.0010466497815178656	0.0	1.335012476425849E-6	1.602014971711019E-5	1.4213350506577827	2.1360199622813585E-5
38-39	0.0012175313785003743	0.0	1.335012476425849E-6	1.602014971711019E-5	1.4932161274259796	2.2695212099239436E-5
40-41	0.00137172531952756	0.0	1.335012476425849E-6	1.602014971711019E-5	1.5652534006539185	2.4030224575665283E-5
42-43	0.0015492819788921978	0.0	1.335012476425849E-6	1.602014971711019E-5	1.636870144951783	2.7367755766729903E-5
44-45	0.0017401887630210942	0.0	1.335012476425849E-6	1.602014971711019E-5	1.7090929849139451	3.5377830625284994E-5
46-47	0.0019337655721028426	0.0	1.335012476425849E-6	1.668765595532311E-5	1.7812043513343256	3.671284310171085E-5
48-49	0.0021540426307131077	0.0	1.335012476425849E-6	1.8690174669961887E-5	1.8532316119686914	3.7380349339923774E-5
50-51	0.0024036899638047414	0.0	1.335012476425849E-6	1.8690174669961887E-5	1.9251761018295186	3.7380349339923774E-5
52-53	0.002651334778181736	0.0	1.335012476425849E-6	1.8690174669961887E-5	1.9969610576994128	3.8715361816349625E-5
54-55	0.0028923045301766023	0.0	1.335012476425849E-6	1.8690174669961887E-5	2.0696224492600805	4.005037429277547E-5
56-57	0.003141951863268236	0.0	1.335012476425849E-6	2.0025187146387734E-5	2.1418579718407686	4.4055411722053014E-5
58-59	0.0033942692213127213	0.0	1.335012476425849E-6	2.1360199622813585E-5	2.2136028773363705	4.605793043669179E-5
60-61	0.003687971966126408	0.0	1.335012476425849E-6	2.1360199622813585E-5	2.285160213579034	4.8060449151330566E-5
62-63	0.004016385035327167	0.0	1.335012476425849E-6	2.2695212099239432E-5	2.357314967904898	4.8060449151330566E-5
64-65	0.004347468129480777	0.0	1.335012476425849E-6	2.4030224575665283E-5	2.4292380975661025	4.8060449151330566E-5
66-67	0.004695238879589711	0.0	1.335012476425849E-6	2.536523705209113E-5	2.500662600067362	5.006296786596934E-5
68-69	0.005087732547658911	0.0	1.335012476425849E-6	2.670024952851698E-5	2.5724922788552167	5.4735511533459813E-5
70-71	0.005470213622154917	0.0	1.335012476425849E-6	2.7367755766729903E-5	2.6440309249232103	5.673803024809858E-5
72-73	0.005869382352606246	0.0	1.335012476425849E-6	2.803526200494283E-5	2.7156963971764645	6.141057391558905E-5
74-75	0.006315944025970692	0.0	1.335012476425849E-6	2.803526200494283E-5	2.7878044260656543	6.274558639201491E-5
76-77	0.0067852009114343775	0.0	1.335012476425849E-6	3.070528695779453E-5	2.860581296205533	6.341309263022783E-5
78-79	0.007294508171190839	0.0	1.335012476425849E-6	3.137279319600745E-5	2.9329022595847123	6.54156113448666E-5
80-81	0.007849873361383992	0.0	1.335012476425849E-6	3.204029943422038E-5	3.005410124710591	7.008815501235707E-5
82-83	0.008429936282391023	0.0	1.335012476425849E-6	3.337531191064623E-5	3.0776529898598994	7.34256862034217E-5
84-85	0.009044709527785128	0.0	1.335012476425849E-6	3.337531191064623E-5	3.1501935627914506	7.409319244163463E-5
86-87	0.009730905940668014	0.0	1.335012476425849E-6	3.337531191064623E-5	3.222399047591419	7.87657361091251E-5
88-89	0.010462492777749379	0.0	1.335012476425849E-6	3.337531191064623E-5	3.294172655855264	7.943324234733801E-5
90-91	0.011214772308215345	0.0	1.335012476425849E-6	3.404281814885915E-5	3.3653895639164393	8.14357610619768E-5
92-93	0.011959041763822755	0.0	1.335012476425849E-6	3.671284310171085E-5	3.437587706147787	8.14357610619768E-5
94-95	0.012808109698829596	0.0	1.335012476425849E-6	3.7380349339923774E-5	3.5097671582044656	8.477329225304141E-5
96-97	0.013759306088283014	0.0	1.335012476425849E-6	3.8047855578136696E-5	3.582198927619188	8.744331720589311E-5
98-99	0.01476256796431704	0.0	1.335012476425849E-6	3.8715361816349625E-5	3.6546373720962926	8.944583592053189E-5
100-101	0.01575114470311038	0.0	1.335012476425849E-6	3.8715361816349625E-5	3.7267040155987132	9.211586087338357E-5
102-103	0.01687856273945201	0.0	1.335012476425849E-6	4.005037429277547E-5	3.7985777497982895	9.411837958802236E-5
104-105	0.018102769180334516	0.0	1.335012476425849E-6	4.138538676920132E-5	3.8710722647994045	9.745591077908698E-5
106-107	0.01938905370137082	0.0	1.335012476425849E-6	4.33879054838401E-5	3.9439325732190595	1.0212845444657745E-4
108-109	0.020835539719578227	0.0	1.335012476425849E-6	4.472291796026594E-5	4.016361005102591	1.0680099811406793E-4
110-111	0.02231339853098164	0.0	1.335012476425849E-6	4.5390424198478865E-5	4.089087144768365	1.114735417815584E-4
112-113	0.023886710734449503	0.0	1.335012476425849E-6	4.5390424198478865E-5	4.161424128297263	1.1748109792547472E-4
114-115	0.025717012839629343	0.0	1.335012476425849E-6	4.6725436674904716E-5	4.233958026166432	1.228211478311781E-4
116-117	0.027664796042734658	0.0	1.335012476425849E-6	4.6725436674904716E-5	4.306489921516886	1.2549117278402982E-4
118-119	0.029871571666266587	0.0	1.335012476425849E-6	4.6725436674904716E-5	4.379383605248452	1.281611977368815E-4
120-121	0.03217179816314832	0.0	1.335012476425849E-6	4.6725436674904716E-5	4.452030979178117	1.2949621021330737E-4
122-123	0.03481445536023329	0.0	1.335012476425849E-6	4.7392942913117644E-5	4.524452735999267	1.3350124764258489E-4
124-125	0.03748982036299069	0.0	1.335012476425849E-6	4.939546162775642E-5	4.596767024316064	1.3950880378650122E-4
126-127	0.04054432890905304	0.0	1.335012476425849E-6	4.939546162775642E-5	4.669680065728539	1.441813474539917E-4
128-129	0.04387718755645017	0.0	1.335012476425849E-6	5.073047410418226E-5	4.742670537864646	1.4618386616863048E-4
130-131	0.047661280420879235	0.0	1.335012476425849E-6	5.073047410418226E-5	4.815803188829491	1.475188786450563E-4
132-133	0.05182518433485146	0.0	1.335012476425849E-6	5.206548658060811E-5	4.888347766798472	1.548614472653985E-4
134-135	0.056296808624639844	0.0	1.335012476425849E-6	5.406800529524689E-5	4.961405657064638	1.608690034093148E-4
136-137	0.06141124142182727	0.0	1.335012476425849E-6	5.607052400988566E-5	5.03434606623288	1.6420653460037945E-4
138-139	0.06713844494569417	0.0	1.335012476425849E-6	5.607052400988566E-5	5.106761147991646	1.6887907826786992E-4
>>END_MODULE
