##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065372_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74905667
Total Bases	11.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61251436423362	32.0	32.0	32.0	32.0	32.0
2	31.575916665957998	32.0	32.0	32.0	32.0	32.0
3	35.5387414012347	37.0	37.0	37.0	32.0	37.0
4	36.213192174098126	37.0	37.0	37.0	37.0	37.0
5	36.345539330155084	37.0	37.0	37.0	37.0	37.0
6	40.0687736082772	41.0	41.0	41.0	37.0	41.0
7	40.09867233409723	41.0	41.0	41.0	37.0	41.0
8	40.234530279264455	41.0	41.0	41.0	37.0	41.0
9	40.23628168213227	41.0	41.0	41.0	41.0	41.0
10-14	40.261051474249605	41.0	41.0	41.0	40.2	41.0
15-19	40.29906898499415	41.0	41.0	41.0	41.0	41.0
20-24	40.31140494083045	41.0	41.0	41.0	41.0	41.0
25-29	40.18445701311224	41.0	41.0	41.0	41.0	41.0
30-34	40.12206020940979	41.0	41.0	41.0	41.0	41.0
35-39	40.066595556782104	41.0	41.0	41.0	37.8	41.0
40-44	40.048455092189485	41.0	41.0	41.0	37.0	41.0
45-49	40.00790479844469	41.0	41.0	41.0	37.0	41.0
50-54	39.94814587793471	41.0	41.0	41.0	37.0	41.0
55-59	39.883587168378064	41.0	41.0	41.0	37.0	41.0
60-64	39.823430093747106	41.0	41.0	41.0	37.0	41.0
65-69	39.7428740631867	41.0	41.0	41.0	37.0	41.0
70-74	39.6700263652949	41.0	41.0	41.0	37.0	41.0
75-79	39.350313081118415	41.0	41.0	41.0	37.0	41.0
80-84	39.46419513492884	41.0	41.0	41.0	37.0	41.0
85-89	39.34943640512539	41.0	41.0	41.0	37.0	41.0
90-94	39.20857478246605	41.0	41.0	41.0	37.0	41.0
95-99	39.088085511607545	41.0	41.0	41.0	37.0	41.0
100-104	38.890935816645225	41.0	41.0	41.0	32.0	41.0
105-109	38.70584312666223	41.0	41.0	41.0	32.0	41.0
110-114	38.44489382625749	41.0	41.0	41.0	32.0	41.0
115-119	38.18382949583774	41.0	37.8	41.0	32.0	41.0
120-124	37.884603961940556	41.0	37.0	41.0	32.0	41.0
125-129	37.46297298173715	41.0	37.0	41.0	27.0	41.0
130-134	37.1269511691285	41.0	37.0	41.0	27.0	41.0
135-139	36.633152821401346	41.0	37.0	41.0	27.0	41.0
140-144	36.19641277341539	41.0	35.0	41.0	22.0	41.0
145-149	35.737849879902946	41.0	32.0	41.0	22.0	41.0
150	35.435289428769124	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	0.0
15	26.0
16	107.0
17	403.0
18	1364.0
19	4111.0
20	10124.0
21	22181.0
22	42341.0
23	74281.0
24	120256.0
25	181115.0
26	258763.0
27	354379.0
28	464802.0
29	593047.0
30	742555.0
31	909537.0
32	1108376.0
33	1346967.0
34	1650438.0
35	2062493.0
36	2665702.0
37	3641806.0
38	5548781.0
39	1.12992E7
40	4.1802509E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.231095636786202	41.77793565869334	42.5863838648222	0.40458483969825954
2	17.57227007760629	41.00769961894065	41.154572375076356	0.2654579283767027
3	15.514112367318797	37.119652112306945	47.037536896147415	0.3286986242268472
4	16.176121894755973	36.654114567860404	46.8364669925441	0.3332965448395246
5	16.516901184525864	36.693545496364116	46.46342312124395	0.32613019786607067
6	16.616917649234736	36.11594700838857	46.925187916689936	0.3419474256867641
7	17.088009976173513	36.30190970755547	46.28443260673351	0.3256477095375051
8	16.35176548124189	35.86703259714648	47.44167754356957	0.3395243780420512
9	16.657300867633044	35.78475897268712	47.22333061857996	0.3346095410998776
10-14	16.568682046446497	36.32926945300414	46.77864573317263	0.3234027673767327
15-19	16.562819187354854	35.909368699749606	47.20369879476307	0.3241133181324692
20-24	16.75216042227536	35.86240981573983	47.05952296674861	0.3259067952361941
25-29	16.875059016467283	35.66461977454865	47.13021668356965	0.33010452541441826
30-34	16.960740740420338	35.66887725322225	47.039910095205606	0.3304719111518109
35-39	16.984390084879752	35.56961142748196	47.113788516227615	0.332209971410677
40-44	16.982402674068766	35.61347450438748	47.071396030643	0.3327267909007565
45-49	16.974899513313805	35.588486521699856	47.10823196873392	0.328381996252416
50-54	16.969483352847874	35.60541197636662	47.09649776081298	0.32860690997253106
55-59	16.96190421932873	35.60635344592004	47.10391841499972	0.3278239197515112
60-64	16.941875122582967	35.608003738070124	47.121516214311185	0.32860492503571487
65-69	16.945223005337972	35.604769109117974	47.122100300307515	0.32790758523654195
70-74	16.921640722871647	35.60572185038874	47.14328135042218	0.32935607631743746
75-79	16.911183998352115	35.61294894365993	47.147622151553634	0.32824490643432275
80-84	16.91150268320481	35.58685592174043	47.17270127360294	0.3289401214518195
85-89	16.910174781559764	35.61077157358834	47.14855490054649	0.33049874430539866
90-94	16.89128738845189	35.59115779022881	47.18671836355396	0.3308364577653387
95-99	16.879215316928942	35.6140736972378	47.1743057551443	0.33240523068895406
100-104	16.849497192900795	35.60114673554641	47.21469782084931	0.334658250703482
105-109	16.83362636120105	35.615422789841574	47.21443271893308	0.3365181300242937
110-114	16.83002760929456	35.597983779696314	47.234792546120076	0.33719606488905407
115-119	16.794434315832447	35.60629513220352	47.26178165730171	0.3374888946623251
120-124	16.769366960007314	35.59574680957143	47.291825039668495	0.3430611907527573
125-129	16.760001562292288	35.60299649540502	47.29111328138872	0.34588866091397497
130-134	16.733722903678146	35.59814476968467	47.32024333349515	0.34788899314203503
135-139	16.714276446935138	35.57827368922549	47.35460105967531	0.3528488041640595
140-144	16.68473303782583	35.569838420172914	47.387445035575396	0.3579835064258606
145-149	16.658473557309936	35.55540989671807	47.422490196390825	0.36362634958117257
150	16.629525873724454	35.572148638626835	47.435623192948334	0.36270229470038046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10110.0
1	12746.0
2	20093.0
3	35611.0
4	70403.5
5	144889.0
6	288675.5
7	543628.0
8	951022.5
9	1531835.0
10	2274250.0
11	3123788.5
12	3995611.5
13	4787569.5
14	5402835.5
15	5783172.5
16	5905326.0
17	5769250.0
18	5416174.0
19	4904672.5
20	4299642.0
21	3668266.5
22	3061081.0
23	2510883.0
24	2036633.0
25	1645061.0
26	1322401.0
27	1056745.5
28	843902.5
29	675986.0
30	544343.0
31	437896.0
32	347744.0
33	273725.5
34	216450.0
35	172445.0
36	138159.5
37	111687.0
38	90436.0
39	73277.5
40	59433.5
41	48460.0
42	39916.5
43	32589.5
44	26602.0
45	21829.5
46	17937.5
47	15017.5
48	13003.0
49	13819.5
50	15897.0
51	15512.5
52	12741.0
53	9870.5
54	8263.5
55	7231.0
56	6383.0
57	5623.5
58	5162.5
59	4827.5
60	4312.5
61	3767.5
62	3328.5
63	2862.5
64	2503.5
65	2176.0
66	1945.0
67	1751.5
68	1470.0
69	1271.5
70	1108.5
71	938.5
72	783.5
73	613.0
74	486.0
75	404.0
76	333.5
77	276.5
78	239.5
79	217.5
80	202.0
81	161.5
82	138.5
83	138.5
84	110.5
85	78.5
86	67.5
87	58.5
88	45.5
89	44.5
90	46.0
91	43.5
92	33.5
93	27.0
94	19.5
95	10.0
96	5.5
97	2.5
98	4.0
99	11.0
100	72.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07852409885089202
2	1.602014971711019E-5
3	0.05357004564154005
4	0.0
5	0.0
6	0.0
7	0.00106133491875855
8	0.0
9	2.0292189641672904E-4
10-14	0.0
15-19	1.7622164688821206E-5
20-24	1.0599999062821241E-4
25-29	9.692190578851665E-4
30-34	1.57264469722965E-4
35-39	7.614911165533043E-4
40-44	7.31052832090795E-4
45-49	0.0010263575918761927
50-54	8.928563442336079E-4
55-59	2.8248864001170966E-4
60-64	3.7006545846524536E-4
65-69	0.0020209418868134504
70-74	0.0012832139923405262
75-79	0.0018044028631371775
80-84	0.0018636774170904852
85-89	6.821913754536088E-4
90-94	4.2586897997984584E-4
95-99	0.001267193842623416
100-104	0.0031095110600910876
105-109	2.1814103864798375E-4
110-114	0.001964337357812994
115-119	0.0012292794882929217
120-124	0.0013515666311335296
125-129	4.664533592631917E-4
130-134	0.0013702568058034915
135-139	8.135566031339124E-4
140-144	0.00198890158737923
145-149	5.233248907589328E-5
150	2.1093197127528414E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.4905667E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.19610621221392
#Duplication Level	Percentage of total
1	74.08854325284668
2	15.873563325743548
3	3.9696234322740143
4	1.4669623330569665
5	0.7600687466104871
6	0.5070930297808289
7	0.35503104389231915
8	0.34526087857307153
9	0.24809137243064242
>10	1.9532656357328295
>50	0.18266251113963494
>100	0.14049181528483973
>500	0.04172080240109967
>1k	0.026015983101126933
>5k	0.04160583713188099
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.468513724068434E-5	0.0	0.0	0.0	0.004187934138547888	0.0017461963191650106
2	1.468513724068434E-5	0.0	0.0	0.0	0.013856094492823887	0.005513601527638756
3	1.468513724068434E-5	0.0	0.0	0.0	0.021244053537364537	0.008330477852897299
4	1.602014971711019E-5	0.0	0.0	0.0	0.029352919319175143	0.01157856320804139
5	1.602014971711019E-5	0.0	0.0	0.0	0.03758460624881693	0.015143046520098406
6	1.7355162193536036E-5	0.0	0.0	0.0	0.04560803123213628	0.019256219959966448
7	1.7355162193536036E-5	0.0	0.0	0.0	0.053476594768190236	0.02346551429813715
8	2.2695212099239432E-5	0.0	0.0	0.0	0.06116626663240313	0.027433171378074772
9	2.2695212099239432E-5	0.0	0.0	0.0	0.06907888558017913	0.0318814329495257
10-11	2.403022457566528E-5	0.0	0.0	0.0	0.08019219694018612	0.038594543187233085
12-13	2.670024952851698E-5	0.0	0.0	0.0	0.09353497913582426	0.047581847178531894
14-15	3.27078056724333E-5	0.0	0.0	0.0	0.10606941127698656	0.05689756423903147
16-17	4.6057930436691794E-5	0.0	0.0	0.0	0.11843696685859563	0.06656906212449853
18-19	6.608311758307953E-5	0.0	0.0	0.0	0.1304594217150486	0.07673317961376674
20-21	1.1547857921083595E-4	0.0	0.0	0.0	0.14184707813896108	0.08667435001947182
22-23	1.7021409074429575E-4	0.0	0.0	0.0	0.15288429378781182	0.09633516780512749
24-25	2.1960955237205218E-4	0.0	0.0	0.0	0.16374662280225072	0.10605806367093694
26-27	2.696725202380215E-4	0.0	0.0	0.0	0.17459960672935465	0.1157349016063097
28-29	3.0171281967224185E-4	0.0	0.0	0.0	0.18542922793812117	0.1252522055507496
30-31	3.491057625853595E-4	0.0	0.0	0.0	0.1963442899453789	0.13452520221200354
32-33	4.0984883026273566E-4	0.0	0.0	0.0	0.20722864666567886	0.14400913084453276
34-35	4.94622122515777E-4	2.0025187146387736E-6	0.0	0.0	0.2180782930615917	0.15320603179463044
36-37	5.587027213842179E-4	4.005037429277547E-6	0.0	0.0	0.22905409813652683	0.16239358765739315
38-39	6.207808015380198E-4	4.005037429277547E-6	0.0	0.0	0.24004392084246445	0.17162786895683074
40-41	6.848614004064605E-4	4.005037429277547E-6	0.0	0.0	0.2510250659673052	0.180854807687648
42-43	7.723047176123537E-4	4.005037429277547E-6	0.0	0.0	0.2621537299707911	0.1899088623027681
44-45	8.597480348182468E-4	4.005037429277547E-6	0.0	0.0	0.2733438045481926	0.19892820659350113
46-47	9.55201426882695E-4	4.005037429277547E-6	0.0	0.0	0.28476149875282464	0.20845819315646705
48-49	0.0010666749686642535	4.005037429277547E-6	0.0	0.0	0.2959509058239879	0.2179167565519442
50-51	0.0011788160166840247	4.005037429277547E-6	0.0	0.0	0.3072424413495978	0.22755688164421525
52-53	0.0012876195335127314	4.005037429277547E-6	0.0	0.0	0.31849859904458233	0.23707418558865512
54-55	0.0014097731751056965	4.005037429277547E-6	0.0	0.0	0.33006581464657403	0.24641927292363608
56-57	0.0015272542730311713	4.005037429277547E-6	0.0	0.0	0.3414734962576329	0.2557703678147609
58-59	0.0016500754208623493	4.005037429277547E-6	0.0	0.0	0.35307341966529715	0.2653717775452157
60-61	0.0018102769180334513	4.005037429277547E-6	0.0	6.675062382129245E-7	0.36444438843325433	0.27475491273577474
62-63	0.001979823502539534	4.005037429277547E-6	0.0	1.335012476425849E-6	0.37588477784998564	0.2842989169297431
64-65	0.0021467000620927655	4.005037429277547E-6	0.0	1.335012476425849E-6	0.38718565846293046	0.2937855155872252
66-67	0.002333601808792384	4.005037429277547E-6	0.0	1.335012476425849E-6	0.3986293854108528	0.30339827292372956
68-69	0.0025678964984051206	4.005037429277547E-6	0.0	1.335012476425849E-6	0.41005709220905806	0.31306109322809983
70-71	0.002794181113159302	4.005037429277547E-6	0.0	1.335012476425849E-6	0.4220121289354516	0.3225737246288722
72-73	0.0030438284462509357	4.005037429277547E-6	0.0	1.335012476425849E-6	0.43400855104861424	0.3320596557801161
74-75	0.0033488787971142422	4.005037429277547E-6	0.0	1.335012476425849E-6	0.4455270386952165	0.34175651890263525
76-77	0.003633236454592948	4.005037429277547E-6	0.0	1.335012476425849E-6	0.45703417873576907	0.3515988983850848
78-79	0.003965654561222985	4.005037429277547E-6	0.0	1.335012476425849E-6	0.46873022304173595	0.3612203333026859
80-81	0.004345465610766139	4.005037429277547E-6	0.0	1.335012476425849E-6	0.48043360991632317	0.3705914533809571
82-83	0.004764659528363855	4.005037429277547E-6	0.0	1.335012476425849E-6	0.4923252335500864	0.38015147772464264
84-85	0.005209218683013663	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5045592878840528	0.38985301339082934
86-87	0.005732543573772596	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5167859996493991	0.3995925969125941
88-89	0.006293916320109665	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5289232655788246	0.4090698504827412
90-91	0.006890666897072019	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5411219420821658	0.4186605801133845
92-93	0.007492090017701864	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5533146110293631	0.4283300754801369
94-95	0.008172278874440836	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5656521288302526	0.43785672451191177
96-97	0.008941913567100336	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5779389161570379	0.4471437388041682
98-99	0.009772291327437215	4.005037429277547E-6	0.0	1.335012476425849E-6	0.5903258294195551	0.4564908286578638
100-101	0.010589986469248048	4.005037429277547E-6	0.0	1.335012476425849E-6	0.6028649341043849	0.4661035859943681
102-103	0.011557203008418575	4.005037429277547E-6	0.0	2.0025187146387736E-6	0.6156610286909268	0.4759633206390112
104-105	0.01260118276498359	4.005037429277547E-6	0.0	2.670024952851698E-6	0.6282795666181038	0.4856101207936644
106-107	0.013695225489414573	4.005037429277547E-6	0.0	2.670024952851698E-6	0.640710535292343	0.495280283666655
108-109	0.014957479785875213	4.005037429277547E-6	0.0	2.670024952851698E-6	0.6528951941646818	0.5051099805305785
110-111	0.016288487224871785	4.005037429277547E-6	0.0	2.670024952851698E-6	0.6652467295965738	0.5149303323071671
112-113	0.0177096080060271	4.005037429277547E-6	0.0	2.670024952851698E-6	0.6776309728341381	0.5241125481200241
114-115	0.01941642145713755	4.005037429277547E-6	0.0	2.670024952851698E-6	0.6902328231053599	0.5335971442587916
116-117	0.021226698375171	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7030502778915245	0.5430757328414151
118-119	0.023245904745765097	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7158056545975353	0.5523734272334828
120-121	0.025388599770428587	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7286144318026031	0.5618513483098682
122-123	0.027923120956923057	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7414212064889563	0.5713759948229284
124-125	0.03049301997404282	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7540851348403319	0.5809340166478993
126-127	0.033458082684184626	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7671408893535385	0.5902831090203096
128-129	0.03666011010889203	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7801592635174052	0.5996448840112458
130-131	0.04030803170072566	4.005037429277547E-6	0.0	2.670024952851698E-6	0.7933111389289145	0.6088337748864849
132-133	0.044309731598812144	4.005037429277547E-6	0.0	2.670024952851698E-6	0.8063949287041259	0.6183697690055947
134-135	0.04864852214719615	4.005037429277547E-6	0.0	2.670024952851698E-6	0.8194086303243251	0.6277869470143027
136-137	0.05362945369674099	4.005037429277547E-6	0.0	4.005037429277547E-6	0.8325925460352686	0.6372361653224448
138-139	0.059275888965784125	4.005037429277547E-6	0.0	4.005037429277547E-6	0.8457223937409168	0.646704073805257
>>END_MODULE
