SUMMARISING RUN PARAMETERS ========================== Input filename: SRR10065372_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 514). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065372_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1432.269 s (19.121 µs/read; 3.14 M reads/minute). === Summary === Total reads processed: 74,905,667 Reads with adapters: 31,025,180 (41.4%) Reads written (passing filters): 74,905,667 (100.0%) Total basepairs processed: 11,235,850,050 bp Quality-trimmed: 43,898,085 bp (0.4%) Total written (filtered): 11,150,474,830 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 31025180 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 41.5% C: 0.1% G: 12.7% T: 45.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 24901388 18726416.8 0 24901388 2 3913180 4681604.2 0 3913180 3 1548563 1170401.0 0 1548563 4 589472 292600.3 0 589472 5 4818 73150.1 0 4818 6 4302 18287.5 0 4302 7 2849 4571.9 0 2849 8 2712 1143.0 0 2712 9 3530 285.7 0 2580 950 10 3894 71.4 1 2328 1566 11 2866 17.9 1 2438 428 12 2407 4.5 1 2233 174 13 2104 1.1 1 2025 79 14 2093 1.1 1 1984 109 15 1860 1.1 1 1783 77 16 1853 1.1 1 1750 103 17 1682 1.1 1 1603 79 18 1616 1.1 1 1532 84 19 1591 1.1 1 1505 86 20 1401 1.1 1 1363 38 21 1424 1.1 1 1354 70 22 1351 1.1 1 1286 65 23 1230 1.1 1 1170 60 24 1251 1.1 1 1195 56 25 1157 1.1 1 1111 46 26 1129 1.1 1 1077 52 27 949 1.1 1 902 47 28 944 1.1 1 912 32 29 1015 1.1 1 971 44 30 906 1.1 1 859 47 31 849 1.1 1 800 49 32 739 1.1 1 700 39 33 851 1.1 1 788 63 34 1009 1.1 1 962 47 35 755 1.1 1 726 29 36 686 1.1 1 657 29 37 684 1.1 1 663 21 38 603 1.1 1 578 25 39 502 1.1 1 476 26 40 501 1.1 1 471 30 41 574 1.1 1 532 42 42 534 1.1 1 502 32 43 530 1.1 1 500 30 44 472 1.1 1 432 40 45 569 1.1 1 537 32 46 356 1.1 1 333 23 47 314 1.1 1 300 14 48 346 1.1 1 320 26 49 388 1.1 1 367 21 50 401 1.1 1 381 20 51 292 1.1 1 271 21 52 323 1.1 1 295 28 53 307 1.1 1 290 17 54 296 1.1 1 286 10 55 290 1.1 1 269 21 56 273 1.1 1 257 16 57 253 1.1 1 241 12 58 230 1.1 1 215 15 59 230 1.1 1 217 13 60 200 1.1 1 195 5 61 206 1.1 1 189 17 62 210 1.1 1 197 13 63 230 1.1 1 209 21 64 202 1.1 1 182 20 65 192 1.1 1 180 12 66 182 1.1 1 165 17 67 203 1.1 1 180 23 68 167 1.1 1 147 20 69 220 1.1 1 200 20 70 251 1.1 1 230 21 71 257 1.1 1 210 47 72 971 1.1 1 918 53 73 295 1.1 1 278 17 74 120 1.1 1 112 8 75 74 1.1 1 62 12 76 45 1.1 1 43 2 77 56 1.1 1 48 8 78 48 1.1 1 39 9 79 60 1.1 1 51 9 80 53 1.1 1 47 6 81 37 1.1 1 34 3 82 60 1.1 1 45 15 83 57 1.1 1 37 20 84 39 1.1 1 28 11 85 30 1.1 1 25 5 86 44 1.1 1 37 7 87 30 1.1 1 19 11 88 41 1.1 1 25 16 89 34 1.1 1 25 9 90 42 1.1 1 30 12 91 36 1.1 1 24 12 92 25 1.1 1 20 5 93 28 1.1 1 23 5 94 28 1.1 1 22 6 95 32 1.1 1 28 4 96 29 1.1 1 22 7 97 33 1.1 1 21 12 98 21 1.1 1 15 6 99 23 1.1 1 17 6 100 39 1.1 1 26 13 101 16 1.1 1 11 5 102 31 1.1 1 20 11 103 17 1.1 1 15 2 104 12 1.1 1 8 4 105 29 1.1 1 20 9 106 19 1.1 1 14 5 107 19 1.1 1 11 8 108 27 1.1 1 18 9 109 15 1.1 1 10 5 110 22 1.1 1 13 9 111 11 1.1 1 9 2 112 17 1.1 1 11 6 113 16 1.1 1 11 5 114 17 1.1 1 11 6 115 18 1.1 1 11 7 116 13 1.1 1 10 3 117 17 1.1 1 15 2 118 15 1.1 1 9 6 119 9 1.1 1 5 4 120 18 1.1 1 11 7 121 9 1.1 1 5 4 122 8 1.1 1 5 3 123 10 1.1 1 6 4 124 12 1.1 1 9 3 125 15 1.1 1 8 7 126 8 1.1 1 6 2 127 7 1.1 1 4 3 128 10 1.1 1 5 5 129 5 1.1 1 2 3 130 9 1.1 1 9 131 7 1.1 1 5 2 132 2 1.1 1 2 133 3 1.1 1 2 1 134 2 1.1 1 2 135 1 1.1 1 1 136 4 1.1 1 2 2 139 2 1.1 1 0 2 140 1 1.1 1 0 1 142 4 1.1 1 0 4 143 4 1.1 1 0 4 144 1 1.1 1 0 1 145 1 1.1 1 0 1 146 1 1.1 1 0 1 147 1 1.1 1 0 1 148 6 1.1 1 0 6 149 2 1.1 1 0 2 150 43 1.1 1 0 43 RUN STATISTICS FOR INPUT FILE: SRR10065372_1.fastq.gz ============================================= 74905667 sequences processed in total