##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065371_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91076777
Total Bases	13.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.84917249542109	32.0	32.0	32.0	32.0	32.0
2	31.267417093602248	32.0	32.0	32.0	32.0	32.0
3	34.98617401667606	37.0	37.0	37.0	32.0	37.0
4	35.5675232556813	37.0	37.0	37.0	32.0	37.0
5	35.8177728445529	37.0	37.0	37.0	37.0	37.0
6	39.30807651438961	41.0	41.0	41.0	37.0	41.0
7	39.3985650150971	41.0	41.0	41.0	37.0	41.0
8	39.513858752379875	41.0	41.0	41.0	37.0	41.0
9	39.51563642837296	41.0	41.0	41.0	37.0	41.0
10-14	39.531167465445115	41.0	41.0	41.0	37.0	41.0
15-19	39.48452508590636	41.0	41.0	41.0	37.0	41.0
20-24	39.422086205356166	41.0	41.0	41.0	37.0	41.0
25-29	39.346271495751324	41.0	41.0	41.0	37.0	41.0
30-34	39.29339979389038	41.0	41.0	41.0	37.0	41.0
35-39	39.22769852516849	41.0	41.0	41.0	37.0	41.0
40-44	39.144765796883654	41.0	41.0	41.0	37.0	41.0
45-49	39.03745210702834	41.0	41.0	41.0	37.0	41.0
50-54	38.89772174085606	41.0	41.0	41.0	37.0	41.0
55-59	38.777432200966004	41.0	41.0	41.0	37.0	41.0
60-64	38.686321882031464	41.0	41.0	41.0	33.0	41.0
65-69	38.56484674682768	41.0	41.0	41.0	32.0	41.0
70-74	38.443777682207624	41.0	41.0	41.0	32.0	41.0
75-79	38.23475341030129	41.0	39.4	41.0	31.0	41.0
80-84	38.38195107628809	41.0	41.0	41.0	32.0	41.0
85-89	38.265868999734145	41.0	41.0	41.0	32.0	41.0
90-94	38.11260636067524	41.0	41.0	41.0	32.0	41.0
95-99	37.9151602850417	41.0	38.6	41.0	29.0	41.0
100-104	37.68134382708778	41.0	37.0	41.0	27.0	41.0
105-109	37.48781996973828	41.0	37.0	41.0	27.0	41.0
110-114	37.25090990868067	41.0	37.0	41.0	27.0	41.0
115-119	36.939667423672674	41.0	37.0	41.0	27.0	41.0
120-124	36.61591764934765	41.0	37.0	41.0	23.0	41.0
125-129	36.296535271554454	41.0	37.0	41.0	22.0	41.0
130-134	35.89529003644914	41.0	36.0	41.0	22.0	41.0
135-139	35.40347259982641	41.0	32.0	41.0	22.0	41.0
140-144	34.88223627193132	39.4	32.0	41.0	22.0	41.0
145-149	34.29458153750873	37.0	32.0	41.0	14.0	41.0
150	33.92191882240189	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	70.0
12	630.0
13	3003.0
14	6824.0
15	14601.0
16	24522.0
17	36305.0
18	54424.0
19	85924.0
20	143927.0
21	237249.0
22	352178.0
23	465353.0
24	572418.0
25	687803.0
26	819378.0
27	970695.0
28	1137845.0
29	1323448.0
30	1510129.0
31	1691321.0
32	1850250.0
33	2021882.0
34	2278152.0
35	2751518.0
36	3627339.0
37	5206418.0
38	8359632.0
39	1.6651396E7
40	3.8192143E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.21981722208523477	53.64900860080786	29.055238049803084	17.07593612730383
2	0.1945131064809795	54.07269358462495	33.62154571244659	12.111247596447477
3	0.20882358624893824	47.84492833633304	36.16810525428151	15.778142823136513
4	0.24969414098274978	47.0424009761839	36.22322629412405	16.484678588709293
5	0.2817368903545889	48.00521789234426	35.61370963242547	16.09933558487568
6	0.2819729451829219	47.75378265033179	35.76780732620631	16.196437078278976
7	0.2825199566199867	48.50771515723717	35.33409180574422	15.875673080398617
8	0.2572450929971749	46.47343688335778	37.233808061959564	16.03550996168548
9	0.2792547527817406	46.516059959124895	37.409976125537995	15.794709162555364
10-14	0.26080920795639806	47.4893175288534	36.55714607249184	15.692727190698363
15-19	0.26864532033496835	47.308608507152314	36.63710193028751	15.785644242225208
20-24	0.2778000816889714	47.40327969300519	36.370366445897034	15.948553779408806
25-29	0.2875865782898851	47.43816040036386	36.20586443282641	16.06838858851985
30-34	0.2805875685752385	47.46222726246464	36.12671045409292	16.130474714867194
35-39	0.2799108326268282	47.4833076667848	36.088693070410486	16.148088430177893
40-44	0.28818315588162674	47.475001221139266	36.04474773384212	16.192067889136993
45-49	0.29047272591616075	47.4490368143367	36.04056984767898	16.219920612068158
50-54	0.2924237618693154	47.44363346634851	36.0295150392036	16.23442773257857
55-59	0.29910989283773237	47.427991584400495	36.00379067693316	16.26910784582862
60-64	0.3002696455041586	47.38608652247625	36.007942912533565	16.30570091948603
65-69	0.30921793804415887	47.36058214757273	35.99933862677714	16.330861287605973
70-74	0.32134361034938325	47.33753454258856	35.98118925518748	16.35993259187458
75-79	0.3204082704384123	47.30665215839849	35.98928242621939	16.383657144943705
80-84	0.338639968861577	47.26599200639757	35.98291754995022	16.412450474790635
85-89	0.3364020355300377	47.26031216345948	35.97562250495218	16.42766329605831
90-94	0.3413726762035111	47.22303845227369	35.96758987145975	16.467999000063045
95-99	0.35226171002029916	47.17619151660122	35.96437627422226	16.50717049915622
100-104	0.3650572803883947	47.13521724801485	35.94941013313681	16.55031533845995
105-109	0.36905067364043276	47.08471130557979	35.96409627669684	16.58214174408294
110-114	0.38417408531538566	47.05064828192442	35.9438594633971	16.6213181693631
115-119	0.3997095125916794	47.018300984740826	35.93671587877937	16.64527362388813
120-124	0.41709898515557675	46.9714606871176	35.93284023934562	16.678600088381202
125-129	0.42090670233059463	46.95197207315295	35.92769730118196	16.6994239233345
130-134	0.44758664131132636	46.90680015083903	35.90357255386597	16.74204065398367
135-139	0.466851984640394	46.86259443564544	35.89478595626465	16.775767623449518
140-144	0.4971327354979707	46.824718844028936	35.87385868532072	16.80428973515237
145-149	0.5183945484684119	46.7565159068031	35.85884142414759	16.866248120580895
150	0.5338231461847787	46.74171639029546	35.842133141424156	16.882327322095605
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6593.0
1	8691.5
2	16875.5
3	39511.5
4	95150.0
5	219365.0
6	461317.0
7	876954.0
8	1512394.5
9	2377226.5
10	3432704.5
11	4573294.0
12	5657174.5
13	6550987.5
14	7144870.5
15	7387696.5
16	7286735.0
17	6890208.5
18	6276459.0
19	5521501.0
20	4716412.5
21	3936712.0
22	3220626.0
23	2595790.5
24	2074801.0
25	1650746.5
26	1304040.0
27	1024839.5
28	805706.0
29	636129.5
30	504903.5
31	401769.0
32	318945.5
33	252585.5
34	201233.5
35	161880.5
36	131414.5
37	107962.5
38	89130.0
39	74044.0
40	62134.0
41	52467.5
42	44568.0
43	38377.0
44	33071.5
45	28448.0
46	24726.5
47	21699.0
48	19462.0
49	18202.5
50	17181.5
51	16023.0
52	14618.5
53	12787.0
54	11327.5
55	10243.5
56	9271.0
57	8540.0
58	7695.5
59	6910.0
60	6307.0
61	5650.0
62	5161.5
63	4810.0
64	4360.5
65	3811.0
66	3420.0
67	3143.0
68	2902.0
69	2654.5
70	2385.5
71	2183.5
72	2015.0
73	1857.0
74	1681.0
75	1530.0
76	1470.5
77	1361.0
78	1187.0
79	1047.0
80	991.5
81	909.5
82	834.5
83	788.5
84	713.5
85	654.5
86	623.5
87	612.5
88	579.0
89	538.5
90	510.0
91	486.0
92	474.0
93	478.0
94	496.5
95	499.0
96	540.5
97	605.5
98	712.0
99	1285.0
100	2778.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0351727751630913
2	7.158795265669096E-4
3	0.001467992219355764
4	0.0032434173642310596
5	0.0077473097230922
6	0.0011177382792102975
7	8.048154800207741E-4
8	0.0015108132339816988
9	8.761838377306654E-4
10-14	2.6131798669160196E-4
15-19	7.762681369368176E-4
20-24	0.0017236007374305748
25-29	0.0018274691472668163
30-34	0.0025490581424505174
35-39	8.491736592743065E-4
40-44	0.0021832129610822744
45-49	0.0021155777174679774
50-54	2.769092279143782E-4
55-59	5.322981510423893E-4
60-64	0.0017242595222709736
65-69	8.990217121978306E-4
70-74	9.57873157940141E-4
75-79	0.0016880263560490288
80-84	0.0018057292475336495
85-89	0.0023277064360764546
90-94	0.0024544127203798617
95-99	0.0011258632922418852
100-104	0.002648315058403966
105-109	0.0017431446876957448
110-114	0.0012503736270772954
115-119	0.003390106788693236
120-124	0.0019249693036458681
125-129	8.500520390615051E-4
130-134	0.0024254261874023055
135-139	0.0014442759651014001
140-144	0.002224716406027411
145-149	9.842245515561008E-4
150	3.9417292950539955E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	9.1076777E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.44762936110702
#Duplication Level	Percentage of total
1	74.22256655827849
2	16.165695088125435
3	4.059185763685435
4	1.3452266892657558
5	0.7010734379933641
6	0.4750212362337443
7	0.3760409201787924
8	0.304029389117559
9	0.2389565498097692
>10	1.7792445992081198
>50	0.16338142206260392
>100	0.1205939157098362
>500	0.019000907614885633
>1k	0.021684338989735947
>5k	0.00829918372643039
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6469621009974914E-5	0.0	0.0	0.0	0.16916386929238833	1.0979747339983277E-6
2	1.6469621009974914E-5	0.0	0.0	0.0	0.21695212161493155	5.489873669991638E-6
3	1.6469621009974914E-5	0.0	0.0	1.0979747339983277E-6	0.2499264988263693	9.881772605984949E-6
4	1.7567595743973243E-5	0.0	0.0	1.0979747339983277E-6	0.28840063148040473	1.4273671541978259E-5
5	1.866557047797157E-5	0.0	0.0	1.0979747339983277E-6	0.3285338039575116	1.7567595743973243E-5
6	1.866557047797157E-5	0.0	0.0	1.0979747339983277E-6	0.36861207769791854	2.4155444147963208E-5
7	1.866557047797157E-5	0.0	0.0	2.1959494679966554E-6	0.408336803573978	2.744936834995819E-5
8	2.1959494679966553E-5	0.0	0.0	2.1959494679966554E-6	0.44536270755386964	3.293924201994983E-5
9	2.305746941396488E-5	0.0	0.0	2.1959494679966554E-6	0.4855848159844304	4.172303989193645E-5
10-11	2.635139361595986E-5	0.0	0.0	2.1959494679966554E-6	0.5456012129195129	5.050683776392307E-5
12-13	3.4586204120947315E-5	0.0	0.0	3.293924201994983E-6	0.6239323774050547	6.478050930590133E-5
14-15	4.501696409393143E-5	0.0	0.0	4.391898935993311E-6	0.7004606673773711	7.795620611388126E-5
16-17	6.972139560889381E-5	0.0	0.0	4.391898935993311E-6	0.7774012468623039	9.277886502285869E-5
18-19	1.0705253656483695E-4	0.0	0.0	4.9408863029924745E-6	0.8531631504702895	1.0815051129883527E-4
20-21	1.7238203323773744E-4	0.0	0.0	5.489873669991638E-6	0.928906388507797	1.213262081068152E-4
22-23	2.393584920116354E-4	0.0	0.0	5.489873669991638E-6	1.0049164344056662	1.2681608177680682E-4
24-25	3.233535591625075E-4	0.0	0.0	6.038861036990802E-6	1.0801721716612787	1.3614886701579263E-4
26-27	4.45777742003321E-4	0.0	0.0	6.587848403989966E-6	1.1565873702359935	1.4273671541978258E-4
28-29	5.747897732481244E-4	0.0	0.0	6.587848403989966E-6	1.2332419273027195	1.498735511907717E-4
30-31	7.125856023649146E-4	0.0	0.0	6.587848403989966E-6	1.309115824333573	1.5701038696176085E-4
32-33	8.695959893266754E-4	0.0	0.0	6.587848403989966E-6	1.385344367203508	1.663431722007466E-4
34-35	0.0010392330857294171	0.0	0.0	6.587848403989966E-6	1.4614834251326219	1.745779827057341E-4
36-37	0.001176479927479208	0.0	1.0979747339983277E-6	6.587848403989966E-6	1.5376477364806178	1.8281279321072152E-4
38-39	0.0013318433523399713	0.0	1.0979747339983277E-6	7.685823137988292E-6	1.614351702410374	1.9214557844970733E-4
40-41	0.0015163031076516904	0.0	1.0979747339983277E-6	7.685823137988292E-6	1.690661495410625	1.9928241422069643E-4
42-43	0.0016925280524584222	0.0	1.0979747339983277E-6	7.685823137988292E-6	1.7669103508131387	2.0422330052368893E-4
44-45	0.0018951043908811134	0.0	1.0979747339983277E-6	7.685823137988292E-6	1.8427172713852182	2.1739899733166885E-4
46-47	0.0020806621209268307	0.0	1.0979747339983277E-6	7.685823137988292E-6	1.9181173923183512	2.2618279520365548E-4
48-49	0.002294767194056505	0.0	1.0979747339983277E-6	7.685823137988292E-6	1.9935652751524136	2.3222165624064628E-4
50-51	0.0024956965703781986	0.0	1.0979747339983277E-6	8.783797871986622E-6	2.0690521360895326	2.470443151496237E-4
52-53	0.0027065077193058774	0.0	1.0979747339983277E-6	8.783797871986622E-6	2.1444604918331707	2.5418115092061284E-4
54-55	0.0029321415271425337	0.0	1.0979747339983277E-6	9.881772605984949E-6	2.2201263226519314	2.668078603615936E-4
56-57	0.0031715000191541694	0.0	1.0979747339983277E-6	9.881772605984949E-6	2.2951317216681923	2.8492444347256605E-4
58-59	0.0034223872458727872	0.0	1.0979747339983277E-6	1.0979747339983276E-5	2.3700190884005483	2.997471023815434E-4
60-61	0.0036804113083623944	0.0	1.0979747339983277E-6	1.0979747339983276E-5	2.4438222050830807	3.1292279918952335E-4
62-63	0.003953258029760979	0.0	1.0979747339983277E-6	1.0979747339983276E-5	2.517847661649248	3.25000521263505E-4
64-65	0.004210184117516587	0.0	1.0979747339983277E-6	1.2077722073981604E-5	2.5921953737998438	3.354312812364891E-4
66-67	0.004481932864181174	0.0	1.0979747339983277E-6	1.2077722073981604E-5	2.665504401852077	3.590377380174531E-4
68-69	0.004817913132784662	0.0	1.0979747339983277E-6	1.2077722073981604E-5	2.7392334052400646	3.793502705964222E-4
70-71	0.005148952515085157	0.0	1.0979747339983277E-6	1.2626709440980769E-5	2.8130184053394864	4.0570166421238205E-4
72-73	0.0055107351899376066	0.0	1.0979747339983277E-6	1.3175696807979931E-5	2.8864635822587354	4.276611588923486E-4
74-75	0.005910397993112998	0.0	1.0979747339983277E-6	1.3175696807979931E-5	2.960111884503774	4.474247041043185E-4
76-77	0.006330373328867357	0.0	1.0979747339983277E-6	1.3175696807979931E-5	3.033381385465584	4.688352114172859E-4
78-79	0.006784934868742666	0.0	1.0979747339983277E-6	1.3175696807979931E-5	3.1065048557877715	5.050683776392307E-4
80-81	0.007238398433883975	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.1794103781252603	5.253809102181997E-4
82-83	0.007725350228412233	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.2515665327067955	5.583201522381496E-4
84-85	0.008255123037566425	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.323881344637393	5.967492679280911E-4
86-87	0.008880968635945473	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.3959672288359526	6.384723078200275E-4
88-89	0.009533714615307477	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.4677901480857187	6.763524361429698E-4
90-91	0.010194146417807472	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.5391689365555834	7.048997792269263E-4
92-93	0.010962179744239301	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.6104329866657445	7.427799075498686E-4
94-95	0.011773583072664066	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.6814834806901438	7.899928211117967E-4
96-97	0.01271784134390263	0.0	1.0979747339983277E-6	1.4273671541978259E-5	3.752124979126128	8.454405451787122E-4
98-99	0.013667589488811182	0.0	1.0979747339983277E-6	1.592063364297575E-5	3.822777457309452	8.860656103366504E-4
100-101	0.014738114854459552	0.0	1.0979747339983277E-6	1.6469621009974914E-5	3.8927151539409435	9.239457386595927E-4
102-103	0.015944789087123712	0.0	1.0979747339983277E-6	1.701860837697408E-5	3.962704455384933	9.656687785515291E-4
104-105	0.01724094826060874	0.0	1.0979747339983277E-6	1.866557047797157E-5	4.032605918850202	0.0010035489068744713
106-107	0.018548087181433748	0.0	1.0979747339983277E-6	1.9214557844970733E-5	4.10201384267254	0.0010557027067393918
108-109	0.020051763579644458	0.0	1.0979747339983277E-6	2.2508482046965714E-5	4.170924383940376	0.0011116994181733066
110-111	0.021717940238486923	0.0	1.0979747339983277E-6	2.4704431514962372E-5	4.239960643315255	0.0011720880285432146
112-113	0.023523010701180173	0.0	1.0979747339983277E-6	2.6900380982959024E-5	4.308580221278581	0.001226437777876132
114-115	0.02556743965588506	0.0	1.0979747339983277E-6	2.744936834995819E-5	4.377690044960637	0.0012846304387780431
116-117	0.02774691950287174	0.0	1.0979747339983277E-6	2.7998355716957353E-5	4.446512199262387	0.0013389801881109604
118-119	0.03020528493229399	0.0	1.0979747339983277E-6	2.8547343083956518E-5	4.515087858236354	0.0014037606974168619
120-121	0.03293649708311483	0.0	1.0979747339983277E-6	2.8547343083956518E-5	4.582974538064736	0.0014701881688237608
122-123	0.03603937368139411	0.0	1.0979747339983277E-6	2.9096330450955682E-5	4.65183786641901	0.0015470464002036435
124-125	0.039275654209854176	0.0	1.0979747339983277E-6	2.9645317817954844E-5	4.71958400548144	0.0016184147579135348
126-127	0.04296265336662056	0.0	1.0979747339983277E-6	3.074329255195317E-5	4.787558523288544	0.0016985669134954129
128-129	0.046999906463532415	0.0	1.0979747339983277E-6	3.074329255195317E-5	4.854716696880918	0.0017677393217373073
130-131	0.05148183932771358	0.0	1.0979747339983277E-6	3.18412672859515E-5	4.922173519600941	0.0018605181867601662
132-133	0.05647542841793798	0.0	1.0979747339983277E-6	3.18412672859515E-5	4.9894777238329375	0.0019565909759850196
134-135	0.062089373232871434	0.0	1.0979747339983277E-6	3.293924201994983E-5	5.056451437670001	0.0020685843988528492
136-137	0.06831873288621093	0.0	1.0979747339983277E-6	3.348822938694899E-5	5.12287506616533	0.002169598074380695
138-139	0.07515088066851552	0.0	1.0979747339983277E-6	3.403721675394816E-5	5.18967200607022	0.002279395547780528
>>END_MODULE
