##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065371_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91076777
Total Bases	13.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.212417178530593	32.0	32.0	32.0	32.0	32.0
2	31.473564430151058	32.0	32.0	32.0	32.0	32.0
3	35.108440475446336	37.0	32.0	37.0	32.0	37.0
4	35.85212989036711	37.0	37.0	37.0	32.0	37.0
5	36.199403685530065	37.0	37.0	37.0	37.0	37.0
6	39.670492347352166	41.0	41.0	41.0	37.0	41.0
7	39.89807041590855	41.0	41.0	41.0	37.0	41.0
8	39.98238229268917	41.0	41.0	41.0	37.0	41.0
9	40.0152000218453	41.0	41.0	41.0	37.0	41.0
10-14	40.03875117144297	41.0	41.0	41.0	37.0	41.0
15-19	40.02466941051285	41.0	41.0	41.0	37.0	41.0
20-24	39.981280466259804	41.0	41.0	41.0	37.0	41.0
25-29	39.85207784636472	41.0	41.0	41.0	37.0	41.0
30-34	39.75521480958862	41.0	41.0	41.0	37.0	41.0
35-39	39.70083702896075	41.0	41.0	41.0	37.0	41.0
40-44	39.64119805425263	41.0	41.0	41.0	37.0	41.0
45-49	39.57907287386773	41.0	41.0	41.0	37.0	41.0
50-54	39.48196345814917	41.0	41.0	41.0	37.0	41.0
55-59	39.40094612043638	41.0	41.0	41.0	37.0	41.0
60-64	39.320587659793894	41.0	41.0	41.0	37.0	41.0
65-69	39.21286498313396	41.0	41.0	41.0	37.0	41.0
70-74	39.12270346808605	41.0	41.0	41.0	37.0	41.0
75-79	38.941628931379505	41.0	40.2	41.0	36.0	41.0
80-84	39.17243831761855	41.0	41.0	41.0	37.0	41.0
85-89	39.074339108420574	41.0	41.0	41.0	37.0	41.0
90-94	38.99719423316879	41.0	41.0	41.0	37.0	41.0
95-99	38.875093742063356	41.0	41.0	41.0	36.0	41.0
100-104	38.71406617517877	41.0	41.0	41.0	32.0	41.0
105-109	38.588577079314085	41.0	41.0	41.0	32.0	41.0
110-114	38.44404407722948	41.0	41.0	41.0	32.0	41.0
115-119	38.24409726971344	41.0	37.8	41.0	32.0	41.0
120-124	38.092569962154016	41.0	37.0	41.0	32.0	41.0
125-129	37.817783841867836	41.0	37.0	41.0	31.0	41.0
130-134	37.58987762819056	41.0	37.0	41.0	27.0	41.0
135-139	37.36672667720774	41.0	37.0	41.0	27.0	41.0
140-144	37.072064980955574	41.0	37.0	41.0	27.0	41.0
145-149	36.77127197419382	41.0	37.0	41.0	27.0	41.0
150	36.576669736567425	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	10.0
14	14.0
15	87.0
16	367.0
17	1355.0
18	2974.0
19	5202.0
20	8836.0
21	14988.0
22	25840.0
23	45045.0
24	77299.0
25	128888.0
26	205231.0
27	311293.0
28	453153.0
29	635926.0
30	860619.0
31	1134852.0
32	1460700.0
33	1860701.0
34	2348429.0
35	2983120.0
36	3872639.0
37	5323668.0
38	8258118.0
39	1.7011214E7
40	4.4046204E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.701044210094697	42.30705069959447	41.50502057889788	0.48688451141295713
2	17.101976393828583	41.66400284880374	40.9715745371081	0.2624462202595802
3	14.258814159931053	37.92171693191714	47.49386356371155	0.32560534444026396
4	15.222277601894799	36.80880867212733	47.623261927812685	0.34565179816518316
5	15.990953434814672	36.9316219874579	46.73925824142855	0.33816633629887893
6	16.009371960977496	36.66588026056302	46.97625718573682	0.3484905927226652
7	16.358136540042022	37.034240389554306	46.29083811440556	0.3167849559981123
8	15.233954037615568	36.141049324936745	48.29921060996424	0.32578602748344393
9	15.710056362666414	35.63826813941824	48.332837908833774	0.31883758908157234
10-14	15.754939496397554	36.600783144806954	47.34189577013724	0.3023815886582469
15-19	15.717563352057926	36.42104815182747	47.56252055895748	0.298867937157124
20-24	15.889451307998954	36.31612644263998	47.49242214717865	0.30200010218242573
25-29	16.01928243442085	36.217812779148574	47.45896297744658	0.30394180898399065
30-34	16.142936195469456	36.07728433341465	47.47495269842498	0.3048267726909133
35-39	16.201342958351294	36.040799968905276	47.453430927437516	0.3044261453059152
40-44	16.224558088399103	36.003049587448565	47.462214227883436	0.31017809626889015
45-49	16.254812925890437	35.987347981116294	47.44524692946418	0.31259216352908636
50-54	16.270085248626998	35.97106421375047	47.446770620414306	0.31207991720821965
55-59	16.291544020510504	35.97617991994488	47.41555546999604	0.3167205895485756
60-64	16.289938874922512	35.96704862592521	47.430123212796985	0.31288928635529367
65-69	16.318048965393235	35.95119695775173	47.413764938494445	0.31698913836058734
70-74	16.32304325268009	35.96139027964351	47.3947461532196	0.3208203144568089
75-79	16.335293100848897	35.937250816132305	47.414174309551505	0.31328177346729186
80-84	16.350215706355982	35.91479155960162	47.415832956172956	0.31915977786945005
85-89	16.383216187156176	35.90799137542448	47.372269532153396	0.33652290526595063
90-94	16.361829833719543	35.90950828471779	47.40613120139473	0.3225306801679363
95-99	16.37777502854114	35.89905844832925	47.39356263240531	0.32960389072429397
100-104	16.37477430143242	35.89121042846906	47.39023730458919	0.3437779655093326
105-109	16.37276427821369	35.88658760480375	47.4043050146277	0.336343102354853
110-114	16.40061673230202	35.86559269182574	47.38615580032656	0.3476347755456812
115-119	16.388509497727437	35.85982937273	47.39636087557764	0.35530025396492976
120-124	16.37900432019011	35.8632302629108	47.40782950000595	0.3499359168931453
125-129	16.40230979280558	35.85875536656967	47.35860504007816	0.38032980054659327
130-134	16.388844348274294	35.85926980937484	47.37399840767589	0.37788743467497105
135-139	16.395154220170806	35.84336705295678	47.370726363354684	0.3907523635177327
140-144	16.393967908542983	35.83944022923502	47.366614871541714	0.3999769906802922
145-149	16.396237110631084	35.8165736694981	47.3724537479481	0.4147354719227137
150	16.388542703543717	35.82833313793042	47.36889341293533	0.41423074559052786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7582.0
1	10870.5
2	21597.0
3	47288.5
4	105708.0
5	229301.0
6	464540.0
7	866430.0
8	1483215.0
9	2325261.0
10	3345608.5
11	4452154.0
12	5522769.5
13	6414046.5
14	7031789.5
15	7320745.5
16	7273632.0
17	6934681.0
18	6360650.0
19	5634057.5
20	4839667.0
21	4050556.5
22	3318396.5
23	2676286.5
24	2137020.0
25	1698973.0
26	1344984.5
27	1057909.0
28	833211.0
29	660284.5
30	526126.0
31	419478.0
32	330842.0
33	259309.0
34	204029.0
35	161264.0
36	128537.0
37	103156.0
38	83003.5
39	67117.0
40	54686.5
41	44355.5
42	35658.5
43	28948.0
44	23595.5
45	19250.0
46	15775.0
47	13001.0
48	10890.5
49	10111.0
50	10549.5
51	10211.5
52	8552.0
53	6886.0
54	5681.0
55	4694.5
56	3978.0
57	3479.5
58	2995.5
59	2570.0
60	2165.0
61	1799.0
62	1498.0
63	1129.5
64	834.0
65	670.5
66	529.5
67	413.5
68	313.5
69	238.5
70	192.0
71	157.0
72	132.0
73	113.5
74	101.5
75	91.5
76	73.0
77	67.5
78	72.0
79	65.5
80	59.0
81	53.5
82	50.5
83	49.0
84	48.0
85	38.5
86	33.5
87	34.0
88	32.0
89	37.0
90	38.5
91	37.5
92	40.5
93	42.5
94	45.5
95	50.5
96	55.5
97	56.5
98	64.0
99	107.5
100	532.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7520138750627945
2	0.0014888537393017323
3	0.008380841144609234
4	4.8201090822526583E-4
5	0.0
6	0.0
7	8.761838377306654E-4
8	1.3944279121778762E-4
9	0.0
10-14	1.471286143557759E-4
15-19	3.2500052126350495E-4
20-24	4.94308225246047E-4
25-29	7.960316821487874E-4
30-34	0.0
35-39	2.6022001195760366E-4
40-44	3.5859854812385377E-4
45-49	2.345274031820428E-4
50-54	7.494775534272584E-4
55-59	5.217575935960053E-4
60-64	3.78142498389024E-4
65-69	3.1402077392352166E-5
70-74	6.168422055602604E-4
75-79	0.002152689263477121
80-84	1.5108132339816988E-4
85-89	5.27247467265997E-4
90-94	1.1858127127181939E-4
95-99	2.0005099653449526E-4
100-104	0.0030312886456225828
105-109	6.063016481138764E-4
110-114	5.525008861479584E-4
115-119	8.096465688503668E-4
120-124	4.853048324272608E-5
125-129	3.8231480237821766E-4
130-134	0.0014807287262701445
135-139	1.2890223377140365E-4
140-144	7.00507880290933E-4
145-149	1.8555773004571736E-4
150	3.293924201994983E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	9.1076777E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.30322427443161
#Duplication Level	Percentage of total
1	72.5149564213138
2	16.9557009787792
3	4.283322932971661
4	1.5480816414081058
5	0.75700036430469
6	0.5310321957268908
7	0.4180725674192757
8	0.31099104776160486
9	0.26500901104208147
>10	2.032426588760553
>50	0.18626263981825172
>100	0.12489242996126766
>500	0.030063576477235163
>1k	0.017780431175853574
>5k	0.024407173079479615
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6469621009974914E-5	0.0	0.0	0.0	0.003516813072996643	0.001760053498599319
2	1.6469621009974914E-5	0.0	0.0	0.0	0.013185578580585916	0.004696037937310847
3	1.6469621009974914E-5	0.0	0.0	0.0	0.021458818201263316	0.006359469659318313
4	1.7567595743973243E-5	0.0	0.0	0.0	0.03015368011979607	0.008423662159235169
5	1.866557047797157E-5	0.0	0.0	0.0	0.038934184067580695	0.010932534426421349
6	1.9763545211969898E-5	0.0	0.0	0.0	0.04797271207785493	0.013785072785349003
7	1.9763545211969898E-5	0.0	0.0	0.0	0.056864111473773384	0.01661565164959669
8	2.5253418881961534E-5	0.0	0.0	0.0	0.06502865159578496	0.019375960130868487
9	2.6351393615959863E-5	0.0	0.0	0.0	0.073528074011666	0.022587536227813596
10-11	3.0194305184954008E-5	0.0	0.0	0.0	0.08580343153776732	0.02738294087855129
12-13	3.623316622194481E-5	0.0	0.0	0.0	0.10139851567211255	0.0342666934733538
14-15	4.1723039891936454E-5	0.0	0.0	0.0	0.11622556648002597	0.04157042140391068
16-17	5.1604812497921397E-5	0.0	0.0	0.0	0.1308516879116177	0.04906080503924727
18-19	6.20355724709055E-5	0.0	0.0	0.0	0.1446224870254247	0.056724119695188605
20-21	1.0650354919783777E-4	0.0	0.0	0.0	0.15833070157939383	0.0642699510545921
22-23	1.2571810704280851E-4	0.0	0.0	0.0	0.17136311268458698	0.0716933582311548
24-25	1.471286143557759E-4	0.0	0.0	0.0	0.18417098795667747	0.07915409654867343
26-27	1.7238203323773744E-4	0.0	0.0	0.0	0.19685698803329416	0.08683937069929473
28-29	1.9873342685369728E-4	0.0	0.0	0.0	0.20945679709329196	0.09438520205869824
30-31	2.2069292153366387E-4	0.0	0.0	0.0	0.22192045728627396	0.1020809069692925
32-33	2.5637710038860944E-4	0.0	0.0	0.0	0.23442748748124892	0.10981504099557673
34-35	3.0633495078553343E-4	0.0	0.0	0.0	0.24727159591956138	0.11754752805975996
36-37	3.4366609174147657E-4	0.0	0.0	0.0	0.2600695894190459	0.12533930575957908
38-39	3.870360937344105E-4	0.0	0.0	1.0979747339983277E-6	0.27281268418183047	0.13326833030114799
40-41	4.326020451953411E-4	0.0	0.0	1.0979747339983277E-6	0.28562330439075595	0.1410271687589472
42-43	4.7212913561928084E-4	0.0	0.0	1.0979747339983277E-6	0.2983938485218905	0.14894850747737812
44-45	5.182440744472106E-4	0.0	0.0	1.0979747339983277E-6	0.311192939996109	0.15681275150964114
46-47	5.676529374771353E-4	0.0	0.0	1.0979747339983277E-6	0.32427695591380007	0.1646440562998842
48-49	6.340804088840342E-4	0.0	0.0	1.0979747339983277E-6	0.3374026948713831	0.17270154388533093
50-51	6.818423098129615E-4	0.0	0.0	1.0979747339983277E-6	0.35042138129240125	0.18075463957184168
52-53	7.416819328158702E-4	0.0	0.0	1.0979747339983277E-6	0.36343622480185045	0.18904599577563005
54-55	8.103053536907658E-4	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.37663443009187736	0.19746361907382823
56-57	8.88261559804647E-4	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.3898040880388203	0.20586751768785144
58-59	9.782954879925098E-4	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.4031724794126169	0.21435760731849351
60-61	0.0010727213151163662	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.41662925775250037	0.22259131984874694
62-63	0.0011781268895802053	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.42999106127789305	0.23116814948337489
64-65	0.0012703567672360649	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.4435461083564694	0.23972521557279086
66-67	0.001377958291167901	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.45717087683065466	0.2484859559753635
68-69	0.0015344196907626628	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.4706249102337031	0.25731806473564606
70-71	0.0016782543809164437	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.4843089693435243	0.2660963727339627
72-73	0.001843499578383192	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.4981132566867183	0.27516674201152286
74-75	0.0020350961694659	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.5120937689747189	0.2842113088828341
76-77	0.0022552401036325647	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.5260034618923768	0.2933349299349932
78-79	0.002503931380883186	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.5399049199995296	0.3024387874419403
80-81	0.0027564655697028015	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.5538437092476385	0.3112401529096709
82-83	0.0030386450763403715	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.567874728373403	0.32031765902300213
84-85	0.0033729783828428624	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.5817915581268318	0.32961530907049996
86-87	0.003796796630166217	1.0979747339983277E-6	0.0	1.0979747339983277E-6	0.5956930162339846	0.33876418354154103
88-89	0.00424092741006854	1.0979747339983277E-6	0.0	2.1959494679966554E-6	0.6096307075073595	0.34795093814090505
90-91	0.00473336907826679	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.6238994381630347	0.3571980813506389
92-93	0.005308707838881914	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.6378508541205844	0.3665918041873616
94-95	0.005916985841516988	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.6518785793221471	0.37580985106664455
96-97	0.006640551191221885	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.6661379771925834	0.38513440149512534
98-99	0.007384978060872752	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.6805274850689984	0.3942580225472845
100-101	0.008237555441822454	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.6948049995225457	0.4033476063826896
102-103	0.009241653336063922	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.709042437898302	0.4127358393457423
104-105	0.010314374651180288	1.0979747339983277E-6	0.0	3.293924201994983E-6	0.7232584657667454	0.42195882711132826
106-107	0.011440896728262574	1.0979747339983277E-6	0.0	3.842911568994147E-6	0.7377094602282643	0.431327296529169
108-109	0.01271674336916863	1.0979747339983277E-6	0.0	4.391898935993311E-6	0.752360286091371	0.4407907407615006
110-111	0.014163325081211427	1.0979747339983277E-6	0.0	4.391898935993311E-6	0.766806888653954	0.45020477613080223
112-113	0.015757584394976997	1.0979747339983277E-6	0.0	4.391898935993311E-6	0.781300704130099	0.45953097352138406
114-115	0.01757473257974423	2.1959494679966554E-6	0.0	4.9408863029924745E-6	0.7960163105025115	0.4690399837051766
116-117	0.019593359128200157	2.1959494679966554E-6	0.0	5.489873669991638E-6	0.8107829727000551	0.4784699397081212
118-119	0.02185683404233771	2.1959494679966554E-6	0.0	6.587848403989966E-6	0.8255435960365616	0.4879097774836718
120-121	0.024384920867368858	2.1959494679966554E-6	0.0	6.587848403989966E-6	0.8402581044342401	0.4972700120910076
122-123	0.02725941872097648	2.1959494679966554E-6	0.0	6.587848403989966E-6	0.8552037365134253	0.5066423244204172
124-125	0.030300259746784845	2.1959494679966554E-6	0.0	6.587848403989966E-6	0.8700944698559107	0.5160706334612609
126-127	0.033774800792522555	2.1959494679966554E-6	0.0	7.1368357709891295E-6	0.8851696629537078	0.5253468730014459
128-129	0.037634181982526674	2.1959494679966554E-6	0.0	7.685823137988292E-6	0.9000225161678701	0.5346351902637047
130-131	0.04190310774831217	2.1959494679966554E-6	0.0	7.685823137988292E-6	0.9148797612809685	0.5440486766456394
132-133	0.04670949214638985	2.1959494679966554E-6	0.0	7.685823137988292E-6	0.9299769138734455	0.5535417661957889
134-135	0.05216917151119654	2.1959494679966554E-6	0.0	7.685823137988292E-6	0.9453040921726952	0.5630024654912854
136-137	0.05824975558807928	2.744936834995819E-6	0.0	7.685823137988292E-6	0.9606449951561198	0.5724631647867819
138-139	0.06494410754126709	3.293924201994983E-6	0.0	7.685823137988292E-6	0.9759683305438004	0.5819161782591407
>>END_MODULE
