##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065370_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73706359
Total Bases	11 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85756498974532	32.0	32.0	32.0	32.0	32.0
2	31.203136597752714	32.0	32.0	32.0	32.0	32.0
3	34.89591940092984	37.0	32.0	37.0	32.0	37.0
4	35.49521307924056	37.0	37.0	37.0	32.0	37.0
5	35.84550798391764	37.0	37.0	37.0	37.0	37.0
6	39.2794448441009	41.0	41.0	41.0	37.0	41.0
7	39.40142883736802	41.0	41.0	41.0	37.0	41.0
8	39.49899543945727	41.0	41.0	41.0	37.0	41.0
9	39.479172224475235	41.0	41.0	41.0	37.0	41.0
10-14	39.5327153821287	41.0	41.0	41.0	37.0	41.0
15-19	39.48310823222186	41.0	41.0	41.0	37.0	41.0
20-24	39.412217599841014	41.0	41.0	41.0	37.0	41.0
25-29	39.35151854943751	41.0	41.0	41.0	37.0	41.0
30-34	39.30657123627556	41.0	41.0	41.0	37.0	41.0
35-39	39.24028196535933	41.0	41.0	41.0	37.0	41.0
40-44	39.160595600713364	41.0	41.0	41.0	37.0	41.0
45-49	39.06179197916967	41.0	41.0	41.0	37.0	41.0
50-54	38.940188023668355	41.0	41.0	41.0	37.0	41.0
55-59	38.82971018552144	41.0	41.0	41.0	37.0	41.0
60-64	38.75416127935448	41.0	41.0	41.0	37.0	41.0
65-69	38.64325548084664	41.0	41.0	41.0	32.0	41.0
70-74	38.536924715003224	41.0	41.0	41.0	32.0	41.0
75-79	38.34581372822934	41.0	40.2	41.0	32.0	41.0
80-84	38.49778535390685	41.0	41.0	41.0	32.0	41.0
85-89	38.396040067587656	41.0	41.0	41.0	32.0	41.0
90-94	38.25801785162118	41.0	41.0	41.0	32.0	41.0
95-99	38.080804398437316	41.0	40.2	41.0	32.0	41.0
100-104	37.86493454655656	41.0	37.0	41.0	28.0	41.0
105-109	37.692517341685544	41.0	37.0	41.0	27.0	41.0
110-114	37.47303465634492	41.0	37.0	41.0	27.0	41.0
115-119	37.18928104968528	41.0	37.0	41.0	27.0	41.0
120-124	36.88866971979989	41.0	37.0	41.0	27.0	41.0
125-129	36.59737990042352	41.0	37.0	41.0	23.0	41.0
130-134	36.21910454157693	41.0	37.0	41.0	22.0	41.0
135-139	35.76081433896361	41.0	35.0	41.0	22.0	41.0
140-144	35.270004546012096	41.0	32.0	41.0	22.0	41.0
145-149	34.71879624931684	37.0	32.0	41.0	22.0	41.0
150	34.365511665011155	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	68.0
12	422.0
13	1558.0
14	4236.0
15	10362.0
16	18102.0
17	27180.0
18	41458.0
19	65595.0
20	110255.0
21	181674.0
22	268382.0
23	356926.0
24	445879.0
25	540308.0
26	646706.0
27	764738.0
28	897336.0
29	1034770.0
30	1169690.0
31	1289176.0
32	1393900.0
33	1512105.0
34	1708098.0
35	2084692.0
36	2762620.0
37	3990787.0
38	6468894.0
39	1.3229274E7
40	3.2681168E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.11045432846927342	52.313167952663385	31.431280674597637	16.1450970442697
2	0.1200625052785891	53.24985321706475	33.69139392401749	12.938690353639181
3	0.18253217947785616	49.93633570204392	33.41337997670726	16.467752141770966
4	0.22597896392345668	48.795367476691794	34.02313800450053	16.955515554884222
5	0.2509650995650163	49.218141605900385	33.872466261711594	16.658427032823006
6	0.2522386906262082	48.72947211163811	34.080785520945064	16.937503676790623
7	0.2415320968523859	49.61025826322436	33.529325778896	16.618883861027253
8	0.23167713088201697	48.15145778124625	34.7646528411308	16.852212246740933
9	0.2520268907013029	48.43174999379627	34.66021122105101	16.65601189445142
10-14	0.2407988799112098	48.80005248082613	34.4270575236396	16.532091115623057
15-19	0.2610148283334903	48.45200961202305	34.60890829364628	16.678067265997186
20-24	0.278834684323816	48.604656237701995	34.263979395144275	16.852529682829914
25-29	0.2920860526686121	48.539015500108995	34.19909794603137	16.969800501191024
30-34	0.28807164467993684	48.6590958901031	34.06868719742546	16.984145267791497
35-39	0.28804592154203934	48.61158091887688	34.09745600848429	17.00291715109678
40-44	0.29585760762912844	48.650729427983066	34.0458150459003	17.007597918487512
45-49	0.29883291588652183	48.601595670859915	34.077028281428454	17.022543131825106
50-54	0.301200158142599	48.63334405184385	34.06490498377309	17.000550806240465
55-59	0.3078215714527106	48.61533721371862	34.05656897236795	17.02027224246072
60-64	0.3083970953237075	48.59847249087414	34.06563957162918	17.027490842172973
65-69	0.3175178780404133	48.5830334976378	34.060858432213706	17.03859019210808
70-74	0.3283945076121308	48.56081824623365	34.06445182544675	17.04633542070747
75-79	0.32720254315923364	48.55907011615294	34.068011344666246	17.045715996021578
80-84	0.34474868078005827	48.5333219394426	34.07279112570763	17.049138254069714
85-89	0.3423072831069404	48.53221237002367	34.072079442516184	17.0534009043532
90-94	0.3462653403979479	48.503551485205776	34.08353406541874	17.066649108977533
95-99	0.3573446543460547	48.49523735923344	34.06902531530263	17.078392671117875
100-104	0.36892577022881856	48.45342765957418	34.06350753009812	17.114139040098873
105-109	0.3721406930625564	48.44680396336094	34.06500690484281	17.116048438733692
110-114	0.38675331703371135	48.43014906613829	34.041614561506016	17.14148305532198
115-119	0.40213901943948255	48.40686848647625	34.03689100971842	17.154101484365846
120-124	0.41898011094525067	48.3762677879789	34.02615753904529	17.178594562030565
125-129	0.4230595302050359	48.377644938486355	34.00985683295051	17.189438698358085
130-134	0.44938491629299104	48.33097497506293	33.988063771556156	17.231576337087926
135-139	0.46814603902249663	48.29944416359756	33.97215152448262	17.260258272897318
140-144	0.4981858065590197	48.26625270777135	33.94835672182531	17.287204763844315
145-149	0.5175730421476349	48.21181265695734	33.91864540167836	17.351968899216665
150	0.5342262968955689	48.19837063265556	33.893349921392456	17.37405314905642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5868.0
1	7081.5
2	11773.5
3	23823.0
4	53027.0
5	119544.0
6	253697.5
7	495119.0
8	883572.5
9	1442815.0
10	2168373.5
11	3014666.0
12	3891100.5
13	4694421.0
14	5331292.5
15	5735004.0
16	5874865.5
17	5755462.0
18	5413631.0
19	4909823.0
20	4314110.0
21	3687858.5
22	3081460.0
23	2528510.5
24	2045654.5
25	1636693.0
26	1300582.5
27	1025957.5
28	805155.0
29	635347.0
30	502943.0
31	396443.5
32	310374.0
33	242085.5
34	189741.5
35	149535.0
36	117985.5
37	94347.0
38	76637.0
39	62168.5
40	51101.5
41	42599.5
42	35632.5
43	30107.0
44	25529.0
45	22081.0
46	19053.0
47	16588.5
48	14775.5
49	13841.0
50	13391.0
51	12638.0
52	11575.0
53	10106.5
54	8854.5
55	7909.5
56	7177.5
57	6560.0
58	5931.0
59	5383.5
60	4930.0
61	4420.5
62	4047.0
63	3730.5
64	3375.5
65	3007.5
66	2662.5
67	2418.5
68	2209.0
69	2040.0
70	1864.0
71	1656.5
72	1489.0
73	1385.5
74	1273.0
75	1150.5
76	1050.0
77	1000.5
78	944.5
79	862.0
80	775.5
81	706.5
82	698.5
83	652.0
84	560.5
85	518.0
86	495.5
87	460.0
88	427.0
89	416.0
90	411.5
91	400.0
92	397.5
93	391.0
94	405.0
95	409.5
96	429.5
97	494.0
98	616.5
99	1046.0
100	2187.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.048819953241755
2	7.923332639453808E-4
3	0.0013553783059613621
4	0.0035220841664421383
5	0.007696757887606415
6	0.0010514696567768323
7	8.506728707084827E-4
8	0.0016267253141618349
9	9.931300500137308E-4
10-14	2.7433182529067814E-4
15-19	7.934186519781829E-4
20-24	0.0017971312353117321
25-29	0.0018554708420748338
30-34	0.0026000470325769315
35-39	9.236652159144097E-4
40-44	0.0022334572244980923
45-49	0.002116506663963689
50-54	3.0173787311892585E-4
55-59	5.608742659503774E-4
60-64	0.0017906189071149206
65-69	9.687088192756884E-4
70-74	0.001005883359399153
75-79	0.0017751521276474938
80-84	0.0018497725549026238
85-89	0.0023126905508926306
90-94	0.0025761684958552898
95-99	0.0011138794686629413
100-104	0.002777507975940041
105-109	0.0017336360353928213
110-114	0.0012587787710419938
115-119	0.0033836971922598974
120-124	0.0019379603325677776
125-129	8.902895339057516E-4
130-134	0.002493679005362346
135-139	0.0015070612835454266
140-144	0.002238341470645701
145-149	0.0010077827884565564
150	4.450090934487756E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.3706359E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.11543167321986
#Duplication Level	Percentage of total
1	75.2283963952831
2	15.69807832365332
3	3.796734768483803
4	1.3086050707872796
5	0.7274515574951469
6	0.4993800174130655
7	0.3452188758203836
8	0.28221272956328064
9	0.23820601692680154
>10	1.5687918279296373
>50	0.11565578644650965
>100	0.1485942261981743
>500	0.006128223774379271
>1k	0.025244420859269635
>5k	0.011301759365839452
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.256164098405675E-5	0.0	0.0	0.0	0.19200242953257263	0.0
2	3.3918376025059115E-5	0.0	0.0	0.0	0.24603033233536878	0.0
3	3.3918376025059115E-5	0.0	0.0	0.0	0.2871611118383965	2.713470082004729E-6
4	3.5275111066061476E-5	0.0	0.0	0.0	0.33097822672260885	4.0702051230070935E-6
5	3.663184610706384E-5	0.0	0.0	2.713470082004729E-6	0.37620227584434063	4.0702051230070935E-6
6	3.798858114806621E-5	0.0	0.0	4.0702051230070935E-6	0.421168545308282	6.7836752050118226E-6
7	4.070205123007094E-5	0.0	0.0	4.0702051230070935E-6	0.46622842949005255	6.7836752050118226E-6
8	5.0199196517087486E-5	0.0	0.0	4.0702051230070935E-6	0.5086128321709664	6.7836752050118226E-6
9	5.562613668109695E-5	0.0	0.0	4.0702051230070935E-6	0.552556123413992	6.7836752050118226E-6
10-11	6.376654692711114E-5	0.0	0.0	4.0702051230070935E-6	0.6173015546731864	9.497145287016552E-6
12-13	6.783675205011823E-5	0.0	0.0	4.0702051230070935E-6	0.7021151322913671	1.3567350410023647E-5
14-15	7.936899989863832E-5	0.0	0.0	4.0702051230070935E-6	0.7848562971344168	1.5602452971527194E-5
16-17	1.1057390584169271E-4	0.0	0.0	4.0702051230070935E-6	0.867292196593241	2.30644956970402E-5
18-19	1.3838697418224117E-4	0.0	0.0	4.0702051230070935E-6	0.949359742488433	2.8491435861049654E-5
20-21	1.9197800830183457E-4	0.0	0.0	4.0702051230070935E-6	1.0317705423489987	3.324000850455793E-5
22-23	2.4760414498293154E-4	0.0	0.0	4.0702051230070935E-6	1.1140070017567956	3.459674354556029E-5
24-25	3.2765151240207106E-4	0.0	0.0	6.7836752050118226E-6	1.1944451088677437	3.866694866856739E-5
26-27	4.2262296527223654E-4	0.0	0.0	6.7836752050118226E-6	1.2761877167206157	4.070205123007094E-5
28-29	5.318401360729268E-4	0.0	0.0	6.7836752050118226E-6	1.3585408553419387	4.20587862710733E-5
30-31	6.790458880216834E-4	0.0	0.0	7.462042725513005E-6	1.4401647488787228	4.3415521312075664E-5
32-33	8.757724689670263E-4	0.0	0.0	8.140410246014187E-6	1.5218415550821063	4.5450623873579215E-5
34-35	0.0010426508790103172	0.0	0.0	8.140410246014187E-6	1.602827104782099	4.748572643508276E-5
36-37	0.0012074941864921042	0.0	0.0	8.140410246014187E-6	1.6841782945756418	5.155593155808985E-5
38-39	0.001377764434137901	0.0	0.0	1.3567350410023645E-5	1.766006376736097	5.291266659909222E-5
40-41	0.0015439644766606908	0.0	0.0	1.3567350410023645E-5	1.8480006589390747	5.4947769160595764E-5
42-43	0.0017366208524830266	0.0	0.0	1.4245717930524827E-5	1.9289896004766698	5.698287172209931E-5
44-45	0.0019374176385513766	0.0	0.0	1.492408545102601E-5	2.0105171658255427	6.0374709324605226E-5
46-47	0.002139571159660729	0.0	0.0	1.492408545102601E-5	2.0916404241321973	6.919348709112058E-5
48-49	0.002345794885893088	0.0	0.0	1.8994290574033105E-5	2.1731286441648816	7.597716229613242E-5
50-51	0.0025811884155069985	0.0	0.0	1.8994290574033105E-5	2.253944601984749	8.343920502164542E-5
52-53	0.0027969092870263742	0.0	0.0	1.8994290574033105E-5	2.33486095819765	9.22579827881608E-5
54-55	0.0030356946542427904	0.0	0.0	1.8994290574033105E-5	2.415914344649693	1.0039839303417497E-4
56-57	0.003296187782115245	0.0	0.0	1.8994290574033105E-5	2.4966733195978383	1.1125227336219388E-4
58-59	0.003573640098000228	0.0	0.0	2.035102561503547E-5	2.5772708430761044	1.2549799129271872E-4
60-61	0.0038653381318157366	0.0	0.0	2.1707760656037832E-5	2.6567890838292527	1.343167690592341E-4
62-63	0.004178065558766781	0.0	0.0	2.2386128176539016E-5	2.7367082397870175	1.45170649387253E-4
64-65	0.004500968498525344	0.0	0.0	2.374286321754138E-5	2.8164469499843294	1.5873799979727665E-4
66-67	0.004829976745968417	0.0	0.0	2.4421230738042563E-5	2.895693165361757	1.7705392285080856E-4
68-69	0.005174587446383019	0.0	0.0	2.4421230738042563E-5	2.9750736975082432	1.8858617069932867E-4
70-71	0.005567362240753203	0.0	0.0	2.5777965779044927E-5	3.0546604533809627	2.028318886298535E-4
72-73	0.005958101932561884	0.0	0.0	2.5777965779044927E-5	3.134660335073667	2.2114781168338542E-4
74-75	0.006413964906338679	0.0	0.0	2.5777965779044927E-5	3.2145713506211857	2.45569042421428E-4
76-77	0.006899676051017525	0.0	0.0	2.5777965779044927E-5	3.294675022544527	3.154408970330498E-4
78-79	0.007406416588831908	0.0	0.0	2.5777965779044927E-5	3.3737048386829147	3.473241704966053E-4
80-81	0.007945040400109847	0.0	0.0	2.5777965779044927E-5	3.4530500957183357	3.7717234139865734E-4
82-83	0.008525044630138357	0.0	0.0	2.5777965779044927E-5	3.53244080337763	4.1312581998522E-4
84-85	0.0091803476549425	0.0	0.0	2.5777965779044927E-5	3.611594082404749	4.4093888832576845E-4
86-87	0.009919089884768287	0.0	0.0	2.781306834054847E-5	3.6904061425690555	4.884246147608513E-4
88-89	0.010691072123098633	0.0	0.0	2.8491435861049654E-5	3.768976703896064	5.270915634294186E-4
90-91	0.011509183352823058	0.0	0.0	2.8491435861049654E-5	3.8469408046597446	5.616883069749789E-4
92-93	0.012386312556831088	0.0	0.0	2.8491435861049654E-5	3.9255011904739456	6.037470932460522E-4
94-95	0.013343489128258255	0.0	0.0	2.8491435861049654E-5	4.004617159287436	6.430924094351208E-4
96-97	0.01444244451147017	0.0	0.0	3.256164098405675E-5	4.083260305939138	6.892214008292012E-4
98-99	0.015628230937306236	0.0	0.0	3.256164098405675E-5	4.1618580019669675	7.448475375102981E-4
100-101	0.016896099833122947	0.0	0.0	3.324000850455793E-5	4.240733150310681	7.950467340273856E-4
102-103	0.018263688754453333	0.0	0.0	3.3918376025059115E-5	4.319269793261665	8.41175725421466E-4
104-105	0.019809688333675524	0.0	0.0	3.3918376025059115E-5	4.39867610337393	8.954451270615606E-4
106-107	0.021490683049477453	0.0	0.0	3.3918376025059115E-5	4.478022717144392	9.429308534966434E-4
108-109	0.0233514451582122	1.3567350410023645E-6	0.0	3.3918376025059115E-5	4.5562690187966	0.001001270460259745
110-111	0.025417752625658797	1.3567350410023645E-6	0.0	3.5275111066061476E-5	4.634104229731386	0.0010752125199943738
112-113	0.027720131990239812	1.3567350410023645E-6	0.0	3.5275111066061476E-5	4.711793591649263	0.0011369439643599815
114-115	0.030027938294984832	1.3567350410023645E-6	0.0	3.5275111066061476E-5	4.789390695584353	0.0012020672463280949
116-117	0.032694601018074976	1.3567350410023645E-6	0.0	3.5275111066061476E-5	4.866601130032756	0.0012699039983782132
118-119	0.03576896262098634	1.3567350410023645E-6	0.0	3.5275111066061476E-5	4.944277602967744	0.0013268868701003125
120-121	0.03910788755689316	1.3567350410023645E-6	0.0	3.7310213627565026E-5	5.022275622107449	0.0013859048443839153
122-123	0.042658463159196344	1.3567350410023645E-6	0.0	3.798858114806621E-5	5.100372004537627	0.001462560374200549
124-125	0.046685931128411864	1.3567350410023645E-6	0.0	3.798858114806621E-5	5.178296759985119	0.0015310754937711683
126-127	0.05096100324261031	1.3567350410023645E-6	0.0	3.798858114806621E-5	5.255306397647454	0.0016138363312723125
128-129	0.05579437182618124	1.3567350410023645E-6	0.0	3.866694866856739E-5	5.332124735669008	0.001708807784142478
130-131	0.06122809566539571	1.3567350410023645E-6	0.0	3.934531618906857E-5	5.40917100517745	0.0017983522968486342
132-133	0.06716788167490406	1.3567350410023645E-6	0.0	4.070205123007094E-5	5.486249157959356	0.0019007857924443127
134-135	0.07354589310265618	1.3567350410023645E-6	0.0	4.070205123007094E-5	5.563171964579067	0.002008646228204001
136-137	0.0806185528714015	1.3567350410023645E-6	0.0	4.070205123007094E-5	5.639617878831865	0.0021273605442917076
138-139	0.0883634748529635	1.3567350410023645E-6	0.0	4.070205123007094E-5	5.716318180904853	0.002233185877489892
>>END_MODULE
