##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065370_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73706359
Total Bases	11 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181716668435623	32.0	32.0	32.0	32.0	32.0
2	31.452719608629696	32.0	32.0	32.0	32.0	32.0
3	35.02277996122424	37.0	32.0	37.0	32.0	37.0
4	35.83673232319073	37.0	37.0	37.0	32.0	37.0
5	36.1772900897194	37.0	37.0	37.0	37.0	37.0
6	39.60734135571668	41.0	41.0	41.0	37.0	41.0
7	39.83435818610983	41.0	41.0	41.0	37.0	41.0
8	39.94884320632362	41.0	41.0	41.0	37.0	41.0
9	39.97955833091687	41.0	41.0	41.0	37.0	41.0
10-14	39.99306048206777	41.0	41.0	41.0	37.0	41.0
15-19	39.98123976521483	41.0	41.0	41.0	37.0	41.0
20-24	39.93701519023617	41.0	41.0	41.0	37.0	41.0
25-29	39.81066440413913	41.0	41.0	41.0	37.0	41.0
30-34	39.71714415034394	41.0	41.0	41.0	37.0	41.0
35-39	39.66404181489958	41.0	41.0	41.0	37.0	41.0
40-44	39.60686983602052	41.0	41.0	41.0	37.0	41.0
45-49	39.54648355645949	41.0	41.0	41.0	37.0	41.0
50-54	39.45123624408038	41.0	41.0	41.0	37.0	41.0
55-59	39.37103899542778	41.0	41.0	41.0	37.0	41.0
60-64	39.292179653047306	41.0	41.0	41.0	37.0	41.0
65-69	39.18583608776551	41.0	41.0	41.0	37.0	41.0
70-74	39.09744709001295	41.0	41.0	41.0	37.0	41.0
75-79	38.92072045778303	41.0	40.2	41.0	36.0	41.0
80-84	39.16382878443365	41.0	41.0	41.0	37.0	41.0
85-89	39.072220957217546	41.0	41.0	41.0	37.0	41.0
90-94	38.999486326546126	41.0	41.0	41.0	37.0	41.0
95-99	38.88467642798635	41.0	41.0	41.0	37.0	41.0
100-104	38.7282806195867	41.0	41.0	41.0	32.0	41.0
105-109	38.61137308654739	41.0	41.0	41.0	32.0	41.0
110-114	38.47291877760507	41.0	41.0	41.0	32.0	41.0
115-119	38.282781503289286	41.0	37.8	41.0	32.0	41.0
120-124	38.136023370792195	41.0	37.0	41.0	32.0	41.0
125-129	37.86974086727035	41.0	37.0	41.0	32.0	41.0
130-134	37.65065230803221	41.0	37.0	41.0	30.0	41.0
135-139	37.43691328722397	41.0	37.0	41.0	27.0	41.0
140-144	37.15062708497105	41.0	37.0	41.0	27.0	41.0
145-149	36.857182211374734	41.0	37.0	41.0	27.0	41.0
150	36.666654284198195	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	11.0
13	10.0
14	18.0
15	57.0
16	301.0
17	979.0
18	2304.0
19	4192.0
20	7154.0
21	11960.0
22	20665.0
23	36317.0
24	62605.0
25	102957.0
26	162632.0
27	246941.0
28	358745.0
29	501557.0
30	682083.0
31	900912.0
32	1168264.0
33	1487826.0
34	1885637.0
35	2407757.0
36	3145158.0
37	4355884.0
38	6827106.0
39	1.4223142E7
40	3.5103185E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.410475894749027	39.38567292824505	44.85720609221159	0.34664508479433476
2	17.1213305617909	38.47508064821618	44.164319661840274	0.23926912815265033
3	15.509947613346748	35.87669869104466	48.328034984354346	0.28531871125424574
4	16.12653983686706	35.13584412410699	48.44260893634987	0.295007102676088
5	16.52611547397152	35.009672638964574	48.17305247705968	0.2911594100042304
6	16.63061120682952	34.54765008810162	48.5168532066548	0.3048854984140514
7	17.07342427786822	34.92828033858426	47.71273006861204	0.28556531493548737
8	16.29253948202475	34.32099521854744	49.092084617015516	0.29438068241229376
9	16.616704672659246	34.18087576405721	48.91155592151825	0.29086364176529195
10-14	16.578804998130877	34.66788372868484	48.472529623447954	0.28078164973633196
15-19	16.587121315418404	34.360883687693274	48.76758283198426	0.28441216490405885
20-24	16.774086071672148	34.341086286455244	48.59447782502816	0.2903498168444595
25-29	16.9105268811239	34.15842535357907	48.63693139118472	0.29411637411231367
30-34	17.01375915204277	34.13923756564884	48.54947020242853	0.29753307987985134
35-39	17.049747783436956	34.0451109837972	48.60650278367965	0.29863844908619225
40-44	17.050088574090843	34.07393833977711	48.57277833388288	0.303194752249173
45-49	17.06319673500452	34.035439923728525	48.594713413310416	0.3066499279565326
50-54	17.056359989708707	34.05923816735802	48.577496300535614	0.30690554239765927
55-59	17.069353719970827	34.04575731391212	48.573106117066054	0.3117828490509995
60-64	17.039701095427223	34.04710363955505	48.60564999537446	0.30754526964326023
65-69	17.057220578318557	34.047912545045484	48.58366512413526	0.3112017525006976
70-74	17.041257917057237	34.041259447463446	48.60113736099054	0.31634527448877325
75-79	17.03992756699859	34.04063405067221	48.61215803599862	0.3072803463305858
80-84	17.03653579600384	34.02745428828772	48.62221640839225	0.3137935073161932
85-89	17.05601291731145	34.02728672201582	48.58681101618721	0.3298893444855236
90-94	17.014825566722084	34.03244031276992	48.63537929427994	0.31735482622804984
95-99	17.020117600955274	34.03634299223984	48.62081189257827	0.3227275142266179
100-104	17.010684771593635	34.02179584287577	48.631627250954956	0.33589213457564243
105-109	16.990355256948188	34.03980319419091	48.64163532017698	0.3282062286839187
110-114	17.000465335465517	34.0166279077056	48.64223528391175	0.3406714729171313
115-119	16.9803358958434	34.02577878561468	48.64728387931206	0.34660143922985615
120-124	16.96085670728988	34.0194076237708	48.67639031855309	0.3433453503862262
125-129	16.977779037556438	34.01328819197622	48.6369992211252	0.3719335493421387
130-134	16.954772487992965	34.01116374849849	48.66355353281126	0.37051023069728767
135-139	16.951042987175725	33.992542327385145	48.673473565119885	0.38294112031924427
140-144	16.935488096570513	33.985249087973926	48.68593768711627	0.3933251283392831
145-149	16.932272467123276	33.9587898794048	48.700732020304464	0.40820563316745867
150	16.922198906729278	33.967054808231936	48.70366741103938	0.40707887399939935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5891.0
1	7537.0
2	12883.0
3	25214.0
4	53560.5
5	116403.0
6	242432.0
7	470222.0
8	840068.0
9	1376794.5
10	2081435.5
11	2911135.5
12	3782000.0
13	4599322.0
14	5264452.5
15	5706568.0
16	5885562.5
17	5798199.0
18	5483929.0
19	4995805.0
20	4405286.0
21	3779272.0
22	3165352.0
23	2599814.0
24	2105843.0
25	1690076.0
26	1344695.0
27	1060107.5
28	832556.0
29	655604.0
30	517468.0
31	408107.5
32	318396.0
33	245204.0
34	189036.0
35	146724.5
36	114717.5
37	90154.5
38	71282.5
39	56730.0
40	44927.0
41	35430.0
42	27960.0
43	22242.5
44	17798.5
45	14357.0
46	11663.5
47	9427.5
48	7801.0
49	7514.5
50	7961.0
51	7790.0
52	6647.0
53	5219.5
54	4200.5
55	3521.5
56	2968.5
57	2488.5
58	2126.0
59	1862.5
60	1586.0
61	1318.5
62	1091.0
63	817.0
64	620.5
65	464.5
66	370.5
67	313.5
68	235.0
69	189.0
70	174.0
71	149.0
72	107.5
73	79.0
74	73.5
75	64.0
76	60.0
77	55.0
78	57.5
79	53.5
80	44.0
81	45.5
82	49.5
83	45.5
84	37.0
85	34.0
86	31.5
87	32.0
88	29.0
89	25.0
90	25.5
91	29.5
92	33.0
93	41.0
94	46.0
95	48.0
96	49.0
97	40.5
98	58.5
99	123.5
100	447.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7594812816625497
2	0.0014530632289135323
3	0.009050779458526774
4	4.572197088177969E-4
5	0.0
6	0.0
7	8.533863407904872E-4
8	1.30246563936227E-4
9	0.0
10-14	1.5982338783007856E-4
15-19	3.106923243895415E-4
20-24	5.038913942282782E-4
25-29	7.70625503289343E-4
30-34	0.0
35-39	2.3959940824101755E-4
40-44	3.636049909886337E-4
45-49	2.1979107664238303E-4
50-54	7.519025597235104E-4
55-59	5.161020095972995E-4
60-64	3.6197690893943085E-4
65-69	3.527511106606148E-5
70-74	5.934359069344342E-4
75-79	0.002187870927120413
80-84	1.5955204082187808E-4
85-89	5.036200472200778E-4
90-94	1.2292019471481423E-4
95-99	2.1029393135536651E-4
100-104	0.003175845383978335
105-109	6.040184402542528E-4
110-114	5.331968711139292E-4
115-119	8.224527818556334E-4
120-124	6.186711786970782E-5
125-129	3.9291046787428473E-4
130-134	0.0014650024972743532
135-139	1.2644770582142037E-4
140-144	7.006179751736211E-4
145-149	1.6932053311709508E-4
150	5.426940164009458E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.3706359E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.4748441748255
#Duplication Level	Percentage of total
1	74.24607957041532
2	16.20893660591205
3	3.927330460787447
4	1.4005581369979183
5	0.7233638696509108
6	0.5063456640922236
7	0.3752868390924605
8	0.2942356427450248
9	0.2783833587351049
>10	1.7239144585287498
>50	0.12213058267007755
>100	0.12403061560207732
>500	0.02965006404139273
>1k	0.030868461081688534
>5k	0.008885669647590874
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.3918376025059115E-5	0.0	0.0	0.0	0.0026863353811846817	0.0022684609885559536
2	3.5275111066061476E-5	0.0	0.0	0.0	0.008335780091918528	0.005538192437371652
3	3.663184610706384E-5	0.0	0.0	0.0	0.01318610786350198	0.007927402844576817
4	3.798858114806621E-5	0.0	0.0	0.0	0.01837833286541803	0.010780616635804789
5	3.934531618906857E-5	0.0	0.0	0.0	0.023528499081063005	0.013849551298552138
6	4.070205123007094E-5	0.0	0.0	0.0	0.02854027832252574	0.017292944832616137
7	4.477225635307803E-5	0.0	0.0	0.0	0.033424524470134255	0.020798748178566247
8	5.426940164009458E-5	0.0	0.0	0.0	0.03806455831036234	0.024193299251154165
9	5.969634180410404E-5	0.0	0.0	0.0	0.04279006645817358	0.027928390819033675
10-11	6.648001700911586E-5	0.0	0.0	0.0	0.04939261753521158	0.033746749042372313
12-13	6.987185461162178E-5	0.0	0.0	0.0	0.05758933228542737	0.04184103029699242
14-15	7.529879477563122E-5	0.0	0.0	0.0	0.06548146001893812	0.050312483893011184
16-17	9.497145287016552E-5	0.0	0.0	0.0	0.07293468396668461	0.05900983387335684
18-19	1.112522733621939E-4	0.0	0.0	0.0	0.08017557888051424	0.06761017729827083
20-21	1.4381391434625064E-4	0.0	0.0	0.0	0.08730115131585864	0.07636111831273609
22-23	1.6280820492028372E-4	0.0	0.0	0.0	0.09433785760601741	0.08478644291736076
24-25	1.845159655763216E-4	0.0	0.0	0.0	0.10090242010190736	0.09321515935958796
26-27	2.0690209375286058E-4	0.0	0.0	0.0	0.10732181194841003	0.10171035581882426
28-29	2.258963843268937E-4	0.0	0.0	0.0	0.11375002257267924	0.1100502061158658
30-31	2.5574455522894574E-4	1.3567350410023645E-6	0.0	0.0	0.12035325201723776	0.11846874704528547
32-33	3.6292662346813255E-4	1.3567350410023645E-6	0.0	0.0	0.1269381655387427	0.12672244466722335
34-35	4.382254182437637E-4	1.3567350410023645E-6	0.0	0.0	0.1334593125133206	0.13513216681887652
36-37	4.8231930707634063E-4	1.3567350410023645E-6	0.0	0.0	0.14015887014579026	0.14357852081663675
38-39	5.216646232654091E-4	1.3567350410023645E-6	0.0	0.0	0.14692897800039206	0.15175149270363497
40-41	5.58296469372473E-4	1.3567350410023645E-6	0.0	0.0	0.15370247769259637	0.15998551766747832
42-43	6.057821958075557E-4	1.3567350410023645E-6	0.0	0.0	0.16050853902578474	0.1681781622125711
44-45	6.580164948861468E-4	1.3567350410023645E-6	0.0	0.0	0.16745773590579885	0.17630500510817526
46-47	7.224614093337591E-4	1.3567350410023645E-6	0.0	0.0	0.1743234935807913	0.18439521615767235
48-49	7.848712212198679E-4	1.3567350410023645E-6	0.0	0.0	0.18133917047781453	0.19253562640368654
50-51	8.737373664055228E-4	1.3567350410023645E-6	0.0	0.0	0.1884308245371339	0.20072420074365632
52-53	9.5242799878366E-4	1.3567350410023645E-6	0.0	0.0	0.19567985986120953	0.20910610982696892
54-55	0.0010412941439693147	1.3567350410023645E-6	0.0	0.0	0.20289836864686261	0.21747241645731002
56-57	0.0011430492720444921	1.3567350410023645E-6	0.0	0.0	0.20997035004808745	0.22588010350640164
58-59	0.001235985622353154	1.3567350410023645E-6	0.0	0.0	0.217185466996138	0.23429253912813683
60-61	0.0013397758529898349	1.3567350410023645E-6	0.0	0.0	0.224338852499823	0.24245669223736854
62-63	0.0014673089468440572	1.3567350410023645E-6	0.0	0.0	0.23156346659316057	0.25080807478225864
64-65	0.0015907718355752725	1.3567350410023645E-6	0.0	0.0	0.23892578929315988	0.25939824269436507
66-67	0.0017488314678520479	1.3567350410023645E-6	0.0	0.0	0.24622366707871163	0.26799180244407406
68-69	0.0019326690659078683	1.3567350410023645E-6	0.0	0.0	0.2537922135049433	0.27676580795423633
70-71	0.002139571159660729	1.3567350410023645E-6	0.0	0.0	0.26126985868342784	0.2854143154731059
72-73	0.0023498650910160953	1.3567350410023645E-6	0.0	0.0	0.2687603928448019	0.29406757156461905
74-75	0.0025913639283145166	1.3567350410023645E-6	0.0	0.0	0.2762210788352739	0.3027031901005991
76-77	0.0028599974664329847	1.3567350410023645E-6	0.0	0.0	0.28363292236426985	0.311566061755947
78-79	0.0031516955002484927	1.3567350410023645E-6	0.0	0.0	0.29118043939736593	0.32025798479612866
80-81	0.0034644229271995376	1.3567350410023645E-6	0.0	0.0	0.2987693368492127	0.328807450657005
82-83	0.0038198875079421576	1.3567350410023645E-6	0.0	0.0	0.3064348898308761	0.3373365654922664
84-85	0.004228264755283869	1.3567350410023645E-6	0.0	0.0	0.31424764856448817	0.3461723024467943
86-87	0.004724151412770233	1.3567350410023645E-6	0.0	0.0	0.32220150774236456	0.3548201315981434
88-89	0.005304155642798744	1.3567350410023645E-6	0.0	0.0	0.33001562321101763	0.36362466364672824
90-91	0.005918078248852314	1.3567350410023645E-6	0.0	0.0	0.33780056887628923	0.37247328958414566
92-93	0.006598480871915	1.3567350410023645E-6	0.0	0.0	0.34568523456707445	0.3811611424192043
94-95	0.0073440067769457995	1.3567350410023645E-6	0.0	0.0	0.3536404504799918	0.38996024752762515
96-97	0.008206211895502802	1.3567350410023645E-6	0.0	0.0	0.36162619293133175	0.3986915158839959
98-99	0.009133540296027918	1.3567350410023645E-6	0.0	0.0	0.36955223904086754	0.40722877112950323
100-101	0.010174834439997234	1.3567350410023645E-6	0.0	0.0	0.3775990345690526	0.415998028067022
102-103	0.011319240447082726	1.3567350410023645E-6	0.0	0.0	0.38559427416567954	0.4249891111837447
104-105	0.012623062821486	1.3567350410023645E-6	0.0	0.0	0.3936926256254226	0.43386826365958464
106-107	0.014064593802551012	1.3567350410023645E-6	0.0	0.0	0.4019341126319915	0.44296788557958755
108-109	0.015702851364561368	1.3567350410023645E-6	0.0	0.0	0.4100989441087437	0.4519806764569662
110-111	0.01752969509727105	1.3567350410023645E-6	0.0	0.0	0.4181871200556793	0.46107012286416155
112-113	0.0195675111288566	1.3567350410023645E-6	0.0	0.0	0.4265025491219828	0.4700890190492248
114-115	0.021667736972328263	1.3567350410023645E-6	0.0	0.0	0.43485732350447537	0.47914793891799756
116-117	0.024074584935066458	1.3567350410023645E-6	0.0	0.0	0.4431490097075613	0.48808203916299814
118-119	0.026914231375884404	1.3567350410023645E-6	6.783675205011823E-7	0.0	0.4515261702182304	0.49688724957910346
120-121	0.030013014209533805	1.3567350410023645E-6	1.3567350410023645E-6	0.0	0.4598368507118904	0.505783361242956
122-123	0.033322090974538573	1.3567350410023645E-6	1.3567350410023645E-6	0.0	0.4682608185814741	0.5148144680433882
124-125	0.037051077234733575	1.3567350410023645E-6	1.3567350410023645E-6	0.0	0.4766386574596637	0.5235877951860299
126-127	0.04109075581931811	1.3567350410023645E-6	1.3567350410023645E-6	6.783675205011823E-7	0.4851562400470766	0.5323889353970124
128-129	0.04565074229212706	1.3567350410023645E-6	1.3567350410023645E-6	1.3567350410023645E-6	0.49366771732680487	0.5412328127617863
130-131	0.05079887340521053	1.3567350410023645E-6	1.3567350410023645E-6	1.3567350410023645E-6	0.5022850199397314	0.5499715431608825
132-133	0.056515476500474	1.3567350410023645E-6	1.3567350410023645E-6	1.3567350410023645E-6	0.5108575502963049	0.5589687315852897
134-135	0.06266623480885822	1.3567350410023645E-6	1.3567350410023645E-6	1.3567350410023645E-6	0.5194171916699888	0.5680005167532425
136-137	0.06950757125311263	1.3567350410023645E-6	1.3567350410023645E-6	1.3567350410023645E-6	0.5281152200178549	0.5768003002291837
138-139	0.0770605152263728	1.3567350410023645E-6	1.3567350410023645E-6	1.3567350410023645E-6	0.5368458100067052	0.5858212315168084
>>END_MODULE
