SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR10065370_1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.2
Python version: could not detect
Number of cores used for trimming: 2
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Illumina adapter for trimming (count: 641). Second best hit was smallRNA (count: 0)
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 4.2 with Python 3.10.8
Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065370_1.fastq.gz
Processing single-end reads on 2 cores ...
Finished in 1425.497 s (19.340 µs/read; 3.10 M reads/minute).

=== Summary ===

Total reads processed:              73,706,359
Reads with adapters:                29,449,287 (40.0%)
Reads written (passing filters):    73,706,359 (100.0%)

Total basepairs processed: 11,055,953,850 bp
Quality-trimmed:              19,451,796 bp (0.2%)
Total written (filtered):  10,996,746,892 bp (99.5%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 29449287 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 40.1%
  C: 0.1%
  G: 12.9%
  T: 46.8%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	23533389	18426589.8	0	23533389
2	3883674	4606647.4	0	3883674
3	1374832	1151661.9	0	1374832
4	565389	287915.5	0	565389
5	5330	71978.9	0	5330
6	5417	17994.7	0	5417
7	4200	4498.7	0	4200
8	3656	1124.7	0	3656
9	4258	281.2	0	3533 725
10	4769	70.3	1	3403 1366
11	3541	17.6	1	3189 352
12	3931	4.4	1	3627 304
13	2706	1.1	1	2582 124
14	2832	1.1	1	2695 137
15	2372	1.1	1	2286 86
16	2522	1.1	1	2409 113
17	2214	1.1	1	2121 93
18	2328	1.1	1	2225 103
19	2110	1.1	1	2000 110
20	1945	1.1	1	1876 69
21	1949	1.1	1	1865 84
22	1825	1.1	1	1754 71
23	1719	1.1	1	1647 72
24	1865	1.1	1	1782 83
25	1477	1.1	1	1416 61
26	1404	1.1	1	1340 64
27	1366	1.1	1	1316 50
28	1321	1.1	1	1245 76
29	1257	1.1	1	1199 58
30	1231	1.1	1	1173 58
31	1133	1.1	1	1083 50
32	1203	1.1	1	1154 49
33	983	1.1	1	949 34
34	978	1.1	1	939 39
35	906	1.1	1	869 37
36	855	1.1	1	813 42
37	799	1.1	1	754 45
38	759	1.1	1	717 42
39	794	1.1	1	758 36
40	792	1.1	1	752 40
41	820	1.1	1	778 42
42	629	1.1	1	599 30
43	789	1.1	1	753 36
44	503	1.1	1	483 20
45	761	1.1	1	729 32
46	287	1.1	1	262 25
47	409	1.1	1	398 11
48	477	1.1	1	450 27
49	417	1.1	1	399 18
50	445	1.1	1	426 19
51	389	1.1	1	370 19
52	394	1.1	1	361 33
53	365	1.1	1	331 34
54	356	1.1	1	334 22
55	417	1.1	1	397 20
56	238	1.1	1	227 11
57	303	1.1	1	289 14
58	274	1.1	1	251 23
59	208	1.1	1	198 10
60	242	1.1	1	233 9
61	229	1.1	1	211 18
62	209	1.1	1	195 14
63	270	1.1	1	252 18
64	233	1.1	1	212 21
65	160	1.1	1	152 8
66	172	1.1	1	152 20
67	181	1.1	1	163 18
68	169	1.1	1	155 14
69	150	1.1	1	141 9
70	210	1.1	1	184 26
71	336	1.1	1	246 90
72	994	1.1	1	936 58
73	251	1.1	1	232 19
74	68	1.1	1	61 7
75	64	1.1	1	55 9
76	41	1.1	1	33 8
77	38	1.1	1	30 8
78	31	1.1	1	25 6
79	22	1.1	1	19 3
80	29	1.1	1	28 1
81	29	1.1	1	26 3
82	25	1.1	1	19 6
83	24	1.1	1	17 7
84	21	1.1	1	13 8
85	21	1.1	1	17 4
86	23	1.1	1	22 1
87	17	1.1	1	14 3
88	16	1.1	1	13 3
89	15	1.1	1	8 7
90	17	1.1	1	15 2
91	11	1.1	1	8 3
92	19	1.1	1	9 10
93	14	1.1	1	9 5
94	13	1.1	1	13
95	13	1.1	1	10 3
96	20	1.1	1	14 6
97	11	1.1	1	7 4
98	16	1.1	1	14 2
99	13	1.1	1	11 2
100	12	1.1	1	9 3
101	14	1.1	1	13 1
102	10	1.1	1	8 2
103	8	1.1	1	5 3
104	12	1.1	1	10 2
105	15	1.1	1	10 5
106	14	1.1	1	6 8
107	4	1.1	1	2 2
108	12	1.1	1	8 4
109	10	1.1	1	8 2
110	8	1.1	1	7 1
111	7	1.1	1	3 4
112	5	1.1	1	4 1
113	5	1.1	1	2 3
114	4	1.1	1	3 1
115	5	1.1	1	3 2
116	12	1.1	1	8 4
117	18	1.1	1	16 2
118	4	1.1	1	4
119	18	1.1	1	12 6
120	8	1.1	1	8
121	3	1.1	1	1 2
122	5	1.1	1	2 3
123	2	1.1	1	0 2
124	3	1.1	1	1 2
125	4	1.1	1	3 1
126	5	1.1	1	3 2
128	2	1.1	1	2
129	2	1.1	1	1 1
130	2	1.1	1	1 1
131	3	1.1	1	3
133	3	1.1	1	3
134	1	1.1	1	1
135	3	1.1	1	2 1
136	2	1.1	1	1 1
138	2	1.1	1	1 1
140	2	1.1	1	2
141	2	1.1	1	2
144	4	1.1	1	1 3
145	3	1.1	1	0 3
146	1	1.1	1	0 1
147	1	1.1	1	1
148	4	1.1	1	0 4
149	4	1.1	1	0 4
150	70	1.1	1	3 67

RUN STATISTICS FOR INPUT FILE: SRR10065370_1.fastq.gz
=============================================
73706359 sequences processed in total