##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065369_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70186712
Total Bases	10.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.779321077186236	32.0	32.0	32.0	32.0	32.0
2	31.188940350988375	32.0	32.0	32.0	32.0	32.0
3	34.241301815078614	37.0	32.0	37.0	32.0	37.0
4	35.072131801814564	37.0	37.0	37.0	32.0	37.0
5	35.669447373457245	37.0	37.0	37.0	32.0	37.0
6	38.86092281969271	41.0	41.0	41.0	37.0	41.0
7	39.17908525192062	41.0	41.0	41.0	37.0	41.0
8	39.20369630650315	41.0	41.0	41.0	37.0	41.0
9	39.15602431981712	41.0	41.0	41.0	37.0	41.0
10-14	39.29261012825334	41.0	41.0	41.0	37.0	41.0
15-19	39.30440549772441	41.0	41.0	41.0	37.0	41.0
20-24	39.25515519518852	41.0	41.0	41.0	37.0	41.0
25-29	39.20192111008135	41.0	41.0	41.0	37.0	41.0
30-34	39.1516732312521	41.0	41.0	41.0	37.0	41.0
35-39	39.08235142572286	41.0	41.0	41.0	37.0	41.0
40-44	39.00890840135096	41.0	41.0	41.0	37.0	41.0
45-49	38.91982456451301	41.0	41.0	41.0	37.0	41.0
50-54	38.7913744014679	41.0	41.0	41.0	37.0	41.0
55-59	38.67727657907668	41.0	41.0	41.0	32.0	41.0
60-64	38.59301681207121	41.0	41.0	41.0	32.0	41.0
65-69	38.477723355953756	41.0	41.0	41.0	32.0	41.0
70-74	38.363387212097926	41.0	41.0	41.0	32.0	41.0
75-79	38.16081259654961	41.0	39.4	41.0	31.0	41.0
80-84	38.3243114936058	41.0	41.0	41.0	32.0	41.0
85-89	38.2152460596815	41.0	41.0	41.0	32.0	41.0
90-94	38.06741700622762	41.0	41.0	41.0	32.0	41.0
95-99	37.87762203193106	41.0	37.0	41.0	28.0	41.0
100-104	37.65011440342155	41.0	37.0	41.0	27.0	41.0
105-109	37.46267735408378	41.0	37.0	41.0	27.0	41.0
110-114	37.232528108739444	41.0	37.0	41.0	27.0	41.0
115-119	36.92890879686742	41.0	37.0	41.0	27.0	41.0
120-124	36.614380930111096	41.0	37.0	41.0	23.0	41.0
125-129	36.303138132471574	41.0	37.0	41.0	22.0	41.0
130-134	35.90941671979163	41.0	36.0	41.0	22.0	41.0
135-139	35.42751069176741	41.0	32.0	41.0	22.0	41.0
140-144	34.91968587444302	39.4	32.0	41.0	22.0	41.0
145-149	34.3449865866348	37.0	32.0	41.0	14.0	41.0
150	33.9783394326835	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	56.0
12	418.0
13	2030.0
14	4975.0
15	11180.0
16	19127.0
17	28660.0
18	43266.0
19	69809.0
20	117278.0
21	193969.0
22	287972.0
23	379978.0
24	469811.0
25	563266.0
26	667856.0
27	786061.0
28	913255.0
29	1047542.0
30	1180866.0
31	1303603.0
32	1416971.0
33	1546949.0
34	1765447.0
35	2170608.0
36	2880029.0
37	4137160.0
38	6647115.0
39	1.3209682E7
40	2.8321773E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.15480855473084162	54.73145352538427	28.644426016648595	16.469311903236296
2	0.15465011229561787	54.74442716468838	32.98386565468391	12.11705706833209
3	0.20333894472736982	48.914600464299255	34.95273144264803	15.92932914832534
4	0.25225918484973336	48.06487115811481	34.88588679349376	16.796982863541697
5	0.27764490775828404	48.92439688766406	34.40773527219545	16.390222932382198
6	0.2869276251021714	48.50469877237042	34.67424525395324	16.53412834857417
7	0.2758492439599526	49.30869493464118	34.121838231846354	16.293617589552518
8	0.26448609224267167	47.4938179220185	35.84426354014244	16.397432445596383
9	0.288882365271437	47.56203971595022	35.93476637109778	16.214311547680555
10-14	0.2652791485498804	48.34753408984424	35.25523156377082	16.131955197835072
15-19	0.2751046919468574	48.15330340159832	35.31382835585817	16.257763550596653
20-24	0.28615790638643096	48.234431797077015	35.03330840176336	16.446101894773197
25-29	0.2955414782456227	48.26688468500603	34.865442818966706	16.572131017781636
30-34	0.2879886547020752	48.31804604127654	34.76408754806318	16.629877755958212
35-39	0.28899625504679843	48.32932894199035	34.73476729142737	16.646907511535495
40-44	0.296366564166487	48.33239276911827	34.692920019930476	16.67832064678477
45-49	0.2982694495478978	48.321449209969344	34.68709566921939	16.693185671263375
50-54	0.29927866690612015	48.32930788643139	34.67725573545114	16.694157711211354
55-59	0.30539290496130167	48.32245510500322	34.65923746059958	16.712914529435903
60-64	0.3056831452866332	48.30181354389042	34.65004146259995	16.742461848223
65-69	0.3136487962991398	48.288154909395644	34.648917779176244	16.749278515128978
70-74	0.32499080371970557	48.27188721121621	34.6331328878104	16.76998909725369
75-79	0.3238497723939732	48.25676716743935	34.64085639934359	16.778526660823083
80-84	0.342034462166751	48.22835662829274	34.628236085815914	16.801372823724588
85-89	0.3385870840478151	48.222293067233906	34.63452995824141	16.804589890476866
90-94	0.34346456744610365	48.19539283799262	34.6363337029552	16.82480889160608
95-99	0.3534445294348642	48.17234004290699	34.62482885712833	16.849386570529816
100-104	0.3654042810151071	48.145428014326505	34.606275127564146	16.882892577094243
105-109	0.36926342133734397	48.11059522206934	34.61701774898373	16.903123607609594
110-114	0.3835256692178186	48.09288659197048	34.593631012540605	16.929956726271094
115-119	0.39868252367589974	48.07317913549681	34.579867172013024	16.94827116881427
120-124	0.41622593629556304	48.04086180282407	34.569292161011944	16.97362009986842
125-129	0.41975524871375225	48.04350522397238	34.55315508183283	16.98358444548104
130-134	0.44556055023268376	48.00078189944671	34.53049539636711	17.023162153953507
135-139	0.46521593504221465	47.964402520834604	34.514559416167785	17.0558221279554
140-144	0.49551512182400204	47.93055012864254	34.49556191232611	17.07837283720735
145-149	0.5166236226531663	47.87205392839288	34.471665749494065	17.1396566994599
150	0.5332158670748673	47.85970204254397	34.44028526553778	17.166796824843377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4674.0
1	5743.0
2	10020.0
3	22275.5
4	52982.0
5	123411.0
6	267010.5
7	525273.0
8	937551.5
9	1520885.0
10	2263488.5
11	3108114.5
12	3961899.5
13	4720623.5
14	5292172.0
15	5618701.0
16	5680972.0
17	5492979.0
18	5095109.0
19	4555964.0
20	3952481.0
21	3336975.5
22	2752722.5
23	2234167.5
24	1789066.5
25	1420513.5
26	1122070.0
27	880022.0
28	687616.5
29	540238.5
30	426225.5
31	336251.0
32	263988.0
33	206101.0
34	161532.0
35	127501.0
36	101833.5
37	82155.5
38	66970.5
39	55036.0
40	45500.0
41	38011.0
42	32184.5
43	27307.5
44	23635.5
45	20614.0
46	17801.5
47	15565.5
48	13844.0
49	12797.0
50	11970.0
51	11022.5
52	9978.5
53	8918.0
54	7992.5
55	7197.0
56	6491.0
57	5854.5
58	5399.5
59	4991.0
60	4503.5
61	4113.5
62	3782.5
63	3486.0
64	3164.5
65	2779.5
66	2544.0
67	2356.5
68	2144.5
69	1957.5
70	1754.0
71	1652.5
72	1507.0
73	1337.5
74	1214.5
75	1125.5
76	1094.5
77	1004.5
78	900.5
79	849.5
80	788.5
81	732.0
82	679.5
83	628.5
84	589.5
85	548.0
86	528.5
87	490.0
88	461.5
89	440.5
90	428.0
91	429.0
92	427.0
93	418.0
94	423.0
95	451.5
96	471.5
97	504.0
98	601.0
99	1130.5
100	2503.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.041861029193104
2	7.636773182935255E-4
3	0.0013449839337109852
4	0.0036303168041266843
5	0.007611127302843307
6	0.00102583520367787
7	7.821993428043757E-4
8	0.0016655574348603194
9	0.0010030388658183618
10-14	2.7498082543031793E-4
15-19	7.964470539665685E-4
20-24	0.001805185004249807
25-29	0.00185419713064775
30-34	0.0025566092909438468
35-39	9.708390385918064E-4
40-44	0.0021858838465035947
45-49	0.002063353530508738
50-54	3.123098286752626E-4
55-59	5.778871647385334E-4
60-64	0.001840519327932045
65-69	9.89076108879413E-4
70-74	0.0010343838303751855
75-79	0.0018068947295892702
80-84	0.0018923809965624263
85-89	0.0022477189129475107
90-94	0.0026079010511277406
95-99	0.001085960544782323
100-104	0.002827030848802263
105-109	0.0017185589203836762
110-114	0.001209915631893399
115-119	0.0033430829470968808
120-124	0.0018824075987488912
125-129	8.850678173954067E-4
130-134	0.002462289443050132
135-139	0.0015216555521221737
140-144	0.002177050265583035
145-149	0.001029824562803284
150	4.502276727252874E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.0186712E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.23299281531365
#Duplication Level	Percentage of total
1	76.8452465079595
2	15.093459898070897
3	3.5152333489101064
4	1.1676792388500148
5	0.5843088543955721
6	0.43432079139580254
7	0.3282609284846514
8	0.23207696931455152
9	0.21919219617469454
>10	1.3189273970413116
>50	0.09582237472201566
>100	0.12624768578624065
>500	0.011331751208351856
>1k	0.018539502134253368
>5k	0.009352555552050116
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.7097253394631167E-5	0.0	0.0	0.0	0.18119241716295245	1.424771116219264E-6
2	1.7097253394631167E-5	0.0	0.0	1.424771116219264E-6	0.23213938273672088	3.4194506789262335E-5
3	2.2796337859508223E-5	0.0	0.0	1.424771116219264E-6	0.2668938245746574	6.981378469474393E-5
4	2.4221108975727484E-5	0.0	0.0	1.424771116219264E-6	0.3075083500136037	7.836241139205952E-5
5	2.5645880091946748E-5	0.0	0.0	1.424771116219264E-6	0.3500035733259595	8.406149585693657E-5
6	2.5645880091946748E-5	0.0	0.0	1.424771116219264E-6	0.3921910460772119	9.261012255425214E-5
7	2.5645880091946748E-5	0.0	0.0	1.424771116219264E-6	0.43401377742271213	1.0115874925156773E-4
8	2.707065120816601E-5	0.0	0.0	1.424771116219264E-6	0.47303683352484155	1.1540646041376037E-4
9	2.992019344060454E-5	0.0	0.0	1.424771116219264E-6	0.5145546638514709	1.225303159948567E-4
10-11	3.276973567304307E-5	0.0	0.0	1.424771116219264E-6	0.5761781517846284	1.2894178601784338E-4
12-13	3.490689234737197E-5	0.0	0.0	1.424771116219264E-6	0.6571578677171828	1.4318949718003602E-4
14-15	4.630506127712607E-5	0.0	0.0	1.424771116219264E-6	0.7360210861565932	1.7097253394631167E-4
16-17	6.411470022986688E-5	0.0	0.0	1.424771116219264E-6	0.8153373818109616	1.8165831731795613E-4
18-19	8.976058032181362E-5	0.0	0.0	1.424771116219264E-6	0.8935088738734478	1.9804318515447765E-4
20-21	1.2822940045973376E-4	0.0	0.0	1.424771116219264E-6	0.9721562394887511	2.037422696193547E-4
22-23	1.7524684729496945E-4	0.0	0.0	1.424771116219264E-6	1.0514027213584245	2.1300328187477994E-4
24-25	2.386491619667267E-4	0.0	0.0	1.424771116219264E-6	1.1286844438588317	2.1941475189776662E-4
26-27	2.999143199641551E-4	0.0	0.0	1.424771116219264E-6	1.207252022291627	2.279633785950822E-4
28-29	3.7970150247243386E-4	0.0	0.0	2.849542232438528E-6	1.2858302865077937	2.3295007750184963E-4
30-31	4.708868539104667E-4	0.0	0.0	2.849542232438528E-6	1.3643237768425454	2.407863186410556E-4
32-33	5.927047843472138E-4	0.0	0.0	2.849542232438528E-6	1.4429725672289648	2.45773017547823E-4
34-35	7.073988592028646E-4	0.0	0.0	4.274313348657792E-6	1.5214340002136018	2.500473308964808E-4
36-37	8.228053196166249E-4	0.0	0.0	4.274313348657792E-6	1.6002459268928284	2.564588009194675E-4
38-39	9.403489367047141E-4	0.0	0.0	4.274313348657792E-6	1.6794240197489234	2.607331142681253E-4
40-41	0.0010614544815833516	0.0	0.0	4.274313348657792E-6	1.758820814971358	2.657198131748927E-4
42-43	0.0012110554487863743	0.0	0.0	7.123855581096319E-6	1.8376163567827484	2.7213128319787943E-4
44-45	0.0013556697170826297	0.0	0.0	7.123855581096319E-6	1.916180373287753	2.8281706656952386E-4
46-47	0.0014995715998207752	0.0	0.0	7.123855581096319E-6	1.99519176792325	2.899409221506202E-4
48-49	0.0016634202781859904	0.0	0.0	7.123855581096319E-6	2.073952402842293	2.9492762105738765E-4
50-51	0.0018286937276674252	0.0	0.0	7.123855581096319E-6	2.152462278044881	3.0490101887092246E-4
52-53	0.0019847061648934346	0.0	0.0	7.123855581096319E-6	2.231275629495224	3.1843634447500544E-4
54-55	0.0021628025544208427	0.0	0.0	7.123855581096319E-6	2.310576252667314	3.2698497117232106E-4
56-57	0.0023501599562036754	0.0	0.0	7.123855581096319E-6	2.3892656490305457	3.3339644119530774E-4
58-59	0.002531818273521632	0.0	0.0	7.123855581096319E-6	2.467050315734978	3.547680079385967E-4
60-61	0.0027398348564896444	0.0	0.0	7.123855581096319E-6	2.5449717604665683	3.75427189123776E-4
62-63	0.002974922090665823	0.0	0.0	7.123855581096319E-6	2.623037819466454	3.868253580535301E-4
64-65	0.0032278189637947423	0.0	0.0	7.123855581096319E-6	2.7010882059840613	3.953739847508457E-4
66-67	0.003501375018108841	0.0	0.0	7.123855581096319E-6	2.7787453556735926	4.074845392387094E-4
68-69	0.0037827673135621452	0.0	0.0	7.123855581096319E-6	2.8566247696572535	4.224446359590117E-4
70-71	0.004089805489107397	0.0	0.0	7.123855581096319E-6	2.933996252737983	4.37404732679314E-4
72-73	0.004420352388070266	0.0	0.0	7.123855581096319E-6	3.011161856392418	4.580639138644933E-4
74-75	0.004786518564938617	0.0	0.0	8.548626697315584E-6	3.0891217699441453	4.844221795145497E-4
76-77	0.005173343922992147	0.0	0.0	8.548626697315584E-6	3.166755410910259	5.164795296294831E-4
78-79	0.005572279835533541	0.0	0.0	8.548626697315584E-6	3.2442864683560044	5.435501808376492E-4
80-81	0.0060581267861643095	0.0	0.0	8.548626697315584E-6	3.3213836829968613	5.8059422985935E-4
82-83	0.006591703569188425	0.0	0.0	9.973397813534848E-6	3.3976060881723598	6.069524955094063E-4
84-85	0.007138103292258512	7.12385558109632E-7	0.0	9.973397813534848E-6	3.474800899634677	6.382974600662302E-4
86-87	0.007732945233280055	1.424771116219264E-6	0.0	9.973397813534848E-6	3.5517912564418177	6.717795812973829E-4
88-89	0.008409711513484205	1.424771116219264E-6	0.0	9.973397813534848E-6	3.6279958519783633	6.91013991366343E-4
90-91	0.009201884254102115	1.424771116219264E-6	0.0	9.973397813534848E-6	3.7040643818733097	7.230713414812765E-4
92-93	0.01004463636934581	1.424771116219264E-6	0.0	9.973397813534848E-6	3.7801756549017425	7.558410771543194E-4
94-95	0.010865304532288105	1.424771116219264E-6	0.0	9.973397813534848E-6	3.8564920949709114	7.886108128273625E-4
96-97	0.011832011734642876	1.424771116219264E-6	0.0	9.973397813534848E-6	3.932359019752913	8.213805485004056E-4
98-99	0.012860696480553185	1.424771116219264E-6	0.0	9.973397813534848E-6	4.0082316436193794	8.812209353816147E-4
100-101	0.014035420265875967	1.424771116219264E-6	0.0	1.068578337164448E-5	4.083488766363638	9.31087924449289E-4
102-103	0.015334099138309827	1.424771116219264E-6	0.0	1.2822940045973374E-5	4.1590764360068615	9.69556744587209E-4
104-105	0.016788790447969694	1.424771116219264E-6	0.0	1.4960096720302271E-5	4.235120744792832	9.98764552469704E-4
106-107	0.018330392795718936	1.424771116219264E-6	0.0	1.5672482278411903E-5	4.310999067743763	0.001041507685956282
108-109	0.02014555119778228	1.424771116219264E-6	0.0	1.5672482278411903E-5	4.386614520423752	0.0010892375183496274
110-111	0.022115297265955413	1.424771116219264E-6	0.0	1.6384867836521535E-5	4.461330230143848	0.0011355425796267533
112-113	0.024265989265888393	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.536270341314749	0.0011832724120200986
114-115	0.026551322136304092	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.611630759964934	0.0012267279310647862
116-117	0.029163639977892113	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.686682715668459	0.0012837187757135569
118-119	0.03201246982477253	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.761449004763181	0.0013478334759434237
120-121	0.03506076762792364	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.836832932136784	0.0014112357906151808
122-123	0.038368373774226666	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.912477592624656	0.001468939020822061
124-125	0.04203644701293316	1.424771116219264E-6	0.0	1.7097253394631167E-5	4.9869339940016	0.001534478492168147
126-127	0.046287251638173335	1.424771116219264E-6	0.0	1.7097253394631167E-5	5.061736614759786	0.0016185399880250836
128-129	0.05079380267877487	1.424771116219264E-6	0.0	1.852202451085043E-5	5.136971653551743	0.0016712565193251964
130-131	0.05585245252691136	1.424771116219264E-6	0.0	2.0659181185179327E-5	5.212123343233403	0.0017489065451591463
132-133	0.061361330047773145	1.424771116219264E-6	0.0	2.137156674328896E-5	5.285887305847865	0.0018215698720863288
134-135	0.06755124816218774	1.424771116219264E-6	0.0	2.2796337859508223E-5	5.359336394045642	0.0019184543079892387
136-137	0.07429967655416028	1.424771116219264E-6	0.0	2.2796337859508223E-5	5.433556283417294	0.001997529104939408
138-139	0.08188444559135352	1.424771116219264E-6	0.0	2.4221108975727484E-5	5.5074306657932635	0.002093701155284208
>>END_MODULE
