##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065369_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70186712
Total Bases	10.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177178509231204	32.0	32.0	32.0	32.0	32.0
2	31.423881189932363	32.0	32.0	32.0	32.0	32.0
3	34.93833005028074	37.0	32.0	37.0	32.0	37.0
4	35.7569020187183	37.0	37.0	37.0	32.0	37.0
5	36.15099355273973	37.0	37.0	37.0	37.0	37.0
6	39.59266440633378	41.0	41.0	41.0	37.0	41.0
7	39.82886669488094	41.0	41.0	41.0	37.0	41.0
8	39.912194775558085	41.0	41.0	41.0	37.0	41.0
9	39.952914491848546	41.0	41.0	41.0	37.0	41.0
10-14	39.97393059814513	41.0	41.0	41.0	37.0	41.0
15-19	39.961406173293895	41.0	41.0	41.0	37.0	41.0
20-24	39.91909789989877	41.0	41.0	41.0	37.0	41.0
25-29	39.785568632991385	41.0	41.0	41.0	37.0	41.0
30-34	39.69127854571674	41.0	41.0	41.0	37.0	41.0
35-39	39.63483366766063	41.0	41.0	41.0	37.0	41.0
40-44	39.57774680483679	41.0	41.0	41.0	37.0	41.0
45-49	39.51688769520932	41.0	41.0	41.0	37.0	41.0
50-54	39.41994923198568	41.0	41.0	41.0	37.0	41.0
55-59	39.33916453302443	41.0	41.0	41.0	37.0	41.0
60-64	39.25757781045506	41.0	41.0	41.0	37.0	41.0
65-69	39.14958622652105	41.0	41.0	41.0	37.0	41.0
70-74	39.06088435087257	41.0	41.0	41.0	37.0	41.0
75-79	38.8798701013377	41.0	40.2	41.0	36.0	41.0
80-84	39.12440248803791	41.0	41.0	41.0	37.0	41.0
85-89	39.03043964504278	41.0	41.0	41.0	37.0	41.0
90-94	38.95538399918207	41.0	41.0	41.0	37.0	41.0
95-99	38.83573694405289	41.0	41.0	41.0	36.0	41.0
100-104	38.676105553997175	41.0	41.0	41.0	32.0	41.0
105-109	38.55475803454078	41.0	41.0	41.0	32.0	41.0
110-114	38.41359889604174	41.0	41.0	41.0	32.0	41.0
115-119	38.2161776234795	41.0	37.0	41.0	32.0	41.0
120-124	38.065978480371044	41.0	37.0	41.0	32.0	41.0
125-129	37.793767281761255	41.0	37.0	41.0	31.0	41.0
130-134	37.56970063507178	41.0	37.0	41.0	27.0	41.0
135-139	37.34680675168257	41.0	37.0	41.0	27.0	41.0
140-144	37.055987540775526	41.0	37.0	41.0	27.0	41.0
145-149	36.755546212223194	41.0	37.0	41.0	27.0	41.0
150	36.56075625825014	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	7.0
14	12.0
15	62.0
16	317.0
17	1009.0
18	2236.0
19	4026.0
20	7004.0
21	11274.0
22	19880.0
23	34400.0
24	59323.0
25	98966.0
26	157274.0
27	239367.0
28	348051.0
29	487595.0
30	663414.0
31	880845.0
32	1140948.0
33	1454901.0
34	1842717.0
35	2355609.0
36	3080049.0
37	4262853.0
38	6687565.0
39	1.386641E7
40	3.2480594E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.813399999672686	41.849002659309114	41.9448940940575	0.3927032469606983
2	17.37756086296081	40.39961463926816	41.98997638648928	0.23284811128175217
3	14.448898897245787	36.91762673206683	48.340331529877936	0.2931428408094549
4	15.485976136202684	35.71408614367009	48.48732872019834	0.31260899992888935
5	16.245334302025714	35.76679158299936	47.68201137560055	0.3058627393743705
6	16.280974951497942	35.28466470975304	48.11389084589117	0.32046949285785037
7	16.6584264276597	35.68376836480846	47.36536399081236	0.2924412167194827
8	15.584838575592453	34.93915462322308	49.173172633328086	0.30283416785637624
9	15.998032505070189	34.52066254364502	49.18135501204274	0.29994993924206054
10-14	16.06569454081532	35.299076126280575	48.35053540956844	0.2846939233356737
15-19	16.081111158866428	35.08104860836329	48.552791625877354	0.2850486068929241
20-24	16.26510593171927	34.98625714310163	48.459143893452584	0.2894930317265126
25-29	16.41154325679389	34.873655067926414	48.421857204167935	0.29294447111176236
30-34	16.53648172035755	34.76434171756044	48.40423355349657	0.2949430085854428
35-39	16.5866101420919	34.716434537951585	48.4003938749835	0.2965614449730159
40-44	16.604713647905918	34.69264605011973	48.400972163518674	0.30166813845567675
45-49	16.631378852053864	34.6741916410738	48.390117075278425	0.30431243159391036
50-54	16.64172719201261	34.66171301589017	48.391984483374515	0.3045753087227059
55-59	16.657860538484815	34.66572487671129	48.367865702936264	0.3085488818676377
60-64	16.655200872476158	34.64621010509935	48.392729108875656	0.3058599135488341
65-69	16.678005616455344	34.643385837942986	48.368484210656916	0.31012433494475666
70-74	16.677080190744476	34.642552378431304	48.36471777031158	0.3156496605126371
75-79	16.684907309657838	34.6279899779339	48.38105988047174	0.30604283193652854
80-84	16.693694569993493	34.60581600183203	48.387755947890966	0.31273348028350884
85-89	16.718297792203604	34.60376392415429	48.34800744119669	0.32993084244540494
90-94	16.688420051913084	34.60729763724581	48.387542470735	0.31673984010611433
95-99	16.698934662316887	34.59866055290462	48.37904378632425	0.3233609984542342
100-104	16.696629068183967	34.590247501968264	48.37522155135852	0.3379018784892507
105-109	16.69176533743831	34.58650442019614	48.39110427328603	0.3306259690795186
110-114	16.706495254389438	34.56269406354427	48.387699538184016	0.3431111438822807
115-119	16.691109712614278	34.559553099761196	48.39913163610717	0.3502055515173579
120-124	16.678570113405204	34.55923879384636	48.414980736699334	0.34721035604909944
125-129	16.70641721941371	34.549675028848235	48.3667688348269	0.3771389169111562
130-134	16.687194675129817	34.55198393765881	48.38429814460186	0.37652324260952086
135-139	16.688047046731665	34.52770927319133	48.39498631182176	0.38925736825524354
140-144	16.690037918892873	34.51529714952733	48.393201262064146	0.4014636695156473
145-149	16.681361593626587	34.502201702946635	48.39996762913936	0.4164690742874208
150	16.6636297668161	34.5021914192384	48.416736519637546	0.41744229430795204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5398.0
1	7201.5
2	12974.5
3	26360.5
4	58337.0
5	129922.0
6	273432.5
7	529885.5
8	939767.0
9	1520076.0
10	2257444.5
11	3098477.5
12	3947669.0
13	4699772.5
14	5270589.0
15	5604914.0
16	5675658.5
17	5499785.5
18	5116069.0
19	4587483.0
20	3985775.0
21	3366082.5
22	2777436.5
23	2254019.5
24	1806972.0
25	1436607.5
26	1136012.0
27	891791.0
28	697617.0
29	547536.5
30	432722.5
31	340981.0
32	265854.5
33	204837.5
34	158080.0
35	123667.0
36	97134.0
37	76646.5
38	60633.0
39	48405.0
40	38598.5
41	30793.5
42	24693.0
43	19632.0
44	15738.0
45	12676.0
46	10165.0
47	8329.0
48	7057.0
49	6431.5
50	6438.5
51	6055.5
52	5154.5
53	4232.5
54	3482.0
55	2939.5
56	2510.0
57	2142.5
58	1799.0
59	1621.5
60	1404.0
61	1180.5
62	986.5
63	733.5
64	570.5
65	453.0
66	364.0
67	289.5
68	228.5
69	201.0
70	172.5
71	142.0
72	115.5
73	91.5
74	87.5
75	74.5
76	69.0
77	66.0
78	54.5
79	57.0
80	56.0
81	50.0
82	48.0
83	43.5
84	30.5
85	26.0
86	29.0
87	35.0
88	36.0
89	27.5
90	20.5
91	16.0
92	18.5
93	28.5
94	35.5
95	40.0
96	45.0
97	50.5
98	55.5
99	78.5
100	417.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7533762231232601
2	0.0014888858164491306
3	0.0089048194763704
4	4.459533593766296E-4
5	0.0
6	0.0
7	9.132782854965481E-4
8	1.624239072489961E-4
9	0.0
10-14	1.5216555521221738E-4
15-19	3.222832264887975E-4
20-24	5.120627391692034E-4
25-29	7.25778406602093E-4
30-34	0.0
35-39	2.533243044637851E-4
40-44	3.530582825991336E-4
45-49	2.2938814971130148E-4
50-54	7.460101564524066E-4
55-59	5.14912281401642E-4
60-64	3.8041388803054343E-4
65-69	3.3909552566018485E-5
70-74	5.949844181331646E-4
75-79	0.002142000896124041
80-84	1.5814959390033828E-4
85-89	5.22606045429226E-4
90-94	1.2566481245053908E-4
95-99	1.9946795627069693E-4
100-104	0.003127942508547772
105-109	5.587952317811952E-4
110-114	5.109229222762281E-4
115-119	7.981567793060316E-4
120-124	5.442625663957587E-5
125-129	3.7984397958405573E-4
130-134	0.0013939960601089278
135-139	1.2737453779000218E-4
140-144	6.918688540360745E-4
145-149	1.8806978734094281E-4
150	7.12385558109632E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.0186712E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.28619351280452
#Duplication Level	Percentage of total
1	75.42795839300605
2	15.782178406206478
3	3.6858514346534905
4	1.2376203617492154
5	0.6924647945729687
6	0.43226244190939483
7	0.3753983170111666
8	0.2712282209261847
9	0.24530773071916567
>10	1.573351069090554
>50	0.10526682976589549
>100	0.11742762749978478
>500	0.0237237801377943
>1k	0.0212219056666353
>5k	0.008738687085270362
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.852202451085043E-5	0.0	0.0	0.0	0.0026130302271461297	0.0017367959906712826
2	1.9946795627069695E-5	0.0	0.0	0.0	0.009253888399844118	0.004607709789853099
3	2.5645880091946748E-5	0.0	0.0	0.0	0.015112547229737731	0.006273267224713419
4	2.707065120816601E-5	0.0	0.0	0.0	0.021274682307386047	0.008493060623783032
5	2.992019344060454E-5	0.0	0.0	0.0	0.027297189815644878	0.011020604583956007
6	2.992019344060454E-5	0.0	0.0	0.0	0.03354481116026635	0.013884394527556725
7	2.992019344060454E-5	0.0	0.0	0.0	0.03936642594113826	0.016825122111433288
8	3.134496455682381E-5	0.0	0.0	0.0	0.04496007734341509	0.019691761597266447
9	3.4194506789262335E-5	0.0	0.0	0.0	0.050600746192527156	0.02276641766606762
10-11	3.704404902170086E-5	0.0	0.0	0.0	0.058728353024999944	0.027477423361846613
12-13	4.2030747928468286E-5	0.0	0.0	0.0	0.06896675826615158	0.03425577194725976
14-15	4.986698906767423E-5	0.0	0.0	0.0	0.07863454267525738	0.04143376883077241
16-17	5.627845909066092E-5	0.0	0.0	0.0	0.08844052988263648	0.048896719937528914
18-19	6.340231467175724E-5	0.0	0.0	0.0	0.09767874580020218	0.056676682617644206
20-21	7.765002583394988E-5	0.0	0.0	0.0	0.10656860517985228	0.06419235025570083
22-23	9.189773699614252E-5	0.0	0.0	0.0	0.11511865664828408	0.07168522155589793
24-25	1.0757021927455441E-4	0.0	0.0	0.0	0.12339087774905312	0.07953072370735931
26-27	1.1754361708808927E-4	0.0	0.0	0.0	0.13162534241524237	0.08724728407280284
28-29	1.403399549475975E-4	0.0	0.0	0.0	0.13993816949282367	0.0949503091126423
30-31	1.6883537727198277E-4	0.0	0.0	0.0	0.14822036399140623	0.1026946525148521
32-33	2.208395230139859E-4	0.0	0.0	0.0	0.1563985501985048	0.11051450878622153
34-35	2.6215788538434457E-4	0.0	0.0	0.0	0.16455892676665063	0.11843837334907498
36-37	2.920780788249491E-4	0.0	0.0	0.0	0.1728325726385359	0.1263714989241838
38-39	3.2627258561421143E-4	0.0	0.0	7.12385558109632E-7	0.1809893872788912	0.13416642170101942
40-41	3.6474140575213157E-4	0.0	0.0	1.424771116219264E-6	0.18921102900503445	0.14200978669580647
42-43	4.1175885258736727E-4	0.0	0.0	1.424771116219264E-6	0.19760008133733348	0.1497698025802947
44-45	4.4452858826041035E-4	0.0	0.0	1.424771116219264E-6	0.20594211622279726	0.1576915299864738
46-47	4.8086025172400156E-4	0.0	0.0	1.424771116219264E-6	0.2144273406054411	0.16571797806969502
48-49	5.143423729551543E-4	0.0	0.0	1.424771116219264E-6	0.22296029482047824	0.1738149523231691
50-51	5.663465186971574E-4	0.0	0.0	1.424771116219264E-6	0.23147828893879513	0.18166544117353722
52-53	6.005410254864197E-4	0.0	0.0	1.424771116219264E-6	0.23994712845360244	0.1896719424611314
54-55	6.489832434378747E-4	0.0	0.0	1.424771116219264E-6	0.2483611142804353	0.19793276539297067
56-57	7.159474859001801E-4	0.0	0.0	1.424771116219264E-6	0.25680359552959253	0.20636812278654684
58-59	7.814869572462663E-4	0.0	0.0	1.424771116219264E-6	0.26538214242034874	0.21468379940636056
60-61	8.555750552896679E-4	0.0	0.0	1.424771116219264E-6	0.2740411888791713	0.2227558401653008
62-63	9.353622377979467E-4	0.0	0.0	1.424771116219264E-6	0.28282276565398873	0.23113135717199573
64-65	0.0010536182404441456	0.0	0.0	1.424771116219264E-6	0.2916278511522238	0.2396265549524531
66-67	0.001184697183136318	0.0	0.0	1.424771116219264E-6	0.3002605393453963	0.248138137600747
68-69	0.0013164885113865997	0.0	0.0	1.424771116219264E-6	0.3090235940957029	0.2568206927829872
70-71	0.00147107617749639	0.0	0.0	1.424771116219264E-6	0.3178008965571717	0.26568134435475477
72-73	0.0016327876991872763	0.0	0.0	1.424771116219264E-6	0.3268018880838869	0.27447218214182767
74-75	0.0018301184987836445	0.0	0.0	1.424771116219264E-6	0.33607216135156753	0.28325090937441266
76-77	0.0020545199495881784	0.0	0.0	1.424771116219264E-6	0.34508740058944487	0.292012539353603
78-79	0.002297443424903563	1.424771116219264E-6	0.0	1.424771116219264E-6	0.3539872333669085	0.30087461569648677
80-81	0.002587384347054183	1.424771116219264E-6	0.0	1.424771116219264E-6	0.36313070200524566	0.3094638483706147
82-83	0.002926479872714368	1.424771116219264E-6	0.0	1.424771116219264E-6	0.37232973671711533	0.3182247659642469
84-85	0.003299057519605705	1.424771116219264E-6	0.0	1.424771116219264E-6	0.3815002760066606	0.32718586389970794
86-87	0.0036801837931943584	1.424771116219264E-6	0.0	1.424771116219264E-6	0.3906928992485073	0.3360792852071486
88-89	0.004155344960453482	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4000372606142314	0.34507030333605027
90-91	0.004734514419196614	1.424771116219264E-6	0.0	1.424771116219264E-6	0.40937093619658377	0.353827659001892
92-93	0.005372099493704734	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4188022086003972	0.3626947220436826
94-95	0.006010396953770964	1.424771116219264E-6	0.0	1.424771116219264E-6	0.42827836129437147	0.37163444841240034
96-97	0.00679259629657534	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4376640410224659	0.3805449669732356
98-99	0.0076289369417960485	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4471694015243227	0.38942556534063033
100-101	0.008587807903011613	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4566441294471808	0.39837170317937104
102-103	0.009686306433616666	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4661687243591066	0.4076797328816315
104-105	0.010946516485912605	1.424771116219264E-6	0.0	1.424771116219264E-6	0.47574033671786764	0.4166572156849291
106-107	0.01229150041962359	1.424771116219264E-6	0.0	1.424771116219264E-6	0.48543946609153027	0.4259267765670516
108-109	0.01389579269648648	1.424771116219264E-6	0.0	1.424771116219264E-6	0.4951792014420051	0.43521842140147554
110-111	0.01563828777162264	1.424771116219264E-6	0.0	1.424771116219264E-6	0.5049395959736652	0.444448088692344
112-113	0.01757526410412273	1.424771116219264E-6	0.0	1.424771116219264E-6	0.5147121010598131	0.45366065872981765
114-115	0.019667540488290718	1.424771116219264E-6	0.0	1.424771116219264E-6	0.524255930381808	0.4628739411528495
116-117	0.02208039037360804	1.424771116219264E-6	0.0	1.424771116219264E-6	0.5340355593235369	0.4718877556196107
118-119	0.024692708215196063	1.424771116219264E-6	1.424771116219264E-6	1.424771116219264E-6	0.5438116263374754	0.4810212508601343
120-121	0.027557922929913	1.424771116219264E-6	1.424771116219264E-6	1.424771116219264E-6	0.5538135195733346	0.49005643689363876
122-123	0.03066962304773587	1.424771116219264E-6	1.424771116219264E-6	1.424771116219264E-6	0.563881664666098	0.49908592384267836
124-125	0.0341254053901257	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.5738308413706572	0.5081930608175519
126-127	0.03813898562451536	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.5839445791391396	0.5171826541753374
128-129	0.0424019008042434	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.5941252811500843	0.5262349374622365
130-131	0.04724897214162134	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.6043416024389345	0.5353890918839452
132-133	0.05255980647732864	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.6143841016516061	0.5445838522824662
134-135	0.05852176121314816	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.6244565210577182	0.5536518080516437
136-137	0.06509850468561627	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.6344434541968571	0.5628700771735824
138-139	0.07249235439323615	1.424771116219264E-6	1.424771116219264E-6	2.849542232438528E-6	0.6444553208305299	0.5722906637940242
>>END_MODULE
