##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065368_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65976313
Total Bases	9.8 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.878878621180302	32.0	32.0	32.0	32.0	32.0
2	31.1466353234986	32.0	32.0	32.0	32.0	32.0
3	34.97348421091673	37.0	32.0	37.0	32.0	37.0
4	35.59595238369867	37.0	37.0	37.0	32.0	37.0
5	35.82651396418591	37.0	37.0	37.0	32.0	37.0
6	39.143821192311854	41.0	41.0	41.0	37.0	41.0
7	39.264630974452906	41.0	41.0	41.0	37.0	41.0
8	39.51505233400963	41.0	41.0	41.0	37.0	41.0
9	39.51427346356866	41.0	41.0	41.0	37.0	41.0
10-14	39.518844591997734	41.0	41.0	41.0	37.0	41.0
15-19	39.5000613568691	41.0	41.0	41.0	37.0	41.0
20-24	39.455470971831964	41.0	41.0	41.0	37.0	41.0
25-29	39.395149453107514	41.0	41.0	41.0	37.0	41.0
30-34	39.33666888902992	41.0	41.0	41.0	37.0	41.0
35-39	39.26743566588815	41.0	41.0	41.0	37.0	41.0
40-44	39.20761819472998	41.0	41.0	41.0	37.0	41.0
45-49	39.12518887498306	41.0	41.0	41.0	37.0	41.0
50-54	39.00373552853735	41.0	41.0	41.0	37.0	41.0
55-59	38.89677924560592	41.0	41.0	41.0	37.0	41.0
60-64	38.79845002251035	41.0	41.0	41.0	37.0	41.0
65-69	38.703431339062554	41.0	41.0	41.0	33.0	41.0
70-74	38.589744746724485	41.0	41.0	41.0	32.0	41.0
75-79	38.383009280921776	41.0	40.2	41.0	32.0	41.0
80-84	38.56169557398577	41.0	41.0	41.0	32.0	41.0
85-89	38.443386883410724	41.0	41.0	41.0	32.0	41.0
90-94	38.27380268430581	41.0	41.0	41.0	32.0	41.0
95-99	38.095647078672	41.0	40.2	41.0	32.0	41.0
100-104	37.88929324680511	41.0	37.0	41.0	30.0	41.0
105-109	37.64884525147685	41.0	37.0	41.0	27.0	41.0
110-114	37.44882285859168	41.0	37.0	41.0	27.0	41.0
115-119	37.19528737533424	41.0	37.0	41.0	27.0	41.0
120-124	36.862702503548505	41.0	37.0	41.0	27.0	41.0
125-129	36.50972047195181	41.0	37.0	41.0	23.0	41.0
130-134	36.150242878834405	41.0	37.0	41.0	22.0	41.0
135-139	35.714865291123495	41.0	34.0	41.0	22.0	41.0
140-144	35.16011702260476	40.2	32.0	41.0	22.0	41.0
145-149	34.585384942623264	37.0	32.0	41.0	20.0	41.0
150	34.20880603012781	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	45.0
12	581.0
13	1885.0
14	4450.0
15	10478.0
16	18453.0
17	26271.0
18	37937.0
19	58143.0
20	93336.0
21	150339.0
22	222811.0
23	299329.0
24	376177.0
25	458013.0
26	551119.0
27	651660.0
28	763448.0
29	883090.0
30	997865.0
31	1109098.0
32	1223049.0
33	1359749.0
34	1584692.0
35	1976104.0
36	2647911.0
37	3828740.0
38	6168847.0
39	1.2428015E7
40	2.8044678E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.182452109210049	54.440515347663585	28.132601629184155	17.2444309139422
2	0.15664062724397695	54.8790810416535	32.70462254306052	12.259655788041998
3	0.1866187383207927	49.015928026481	34.76277448325471	16.034678751943495
4	0.23326491746601175	48.18493829195356	34.695705867419136	16.8860909231613
5	0.2565742782217155	49.037554684857945	34.17274188625792	16.533129150662415
6	0.2684236530868311	48.67842092756275	34.379874761698524	16.673280657651894
7	0.26696407045839826	49.467346559356606	33.88186065740522	16.383828712779774
8	0.23970500820618226	47.583588872307416	35.734427355563696	16.442278763922697
9	0.2529060704383762	47.620513179467274	35.89329863255807	16.233282117536277
10-14	0.24982454297255968	48.537078427013924	35.06882363457838	16.144273395435143
15-19	0.26200467099292657	48.34235559538032	35.12076826353657	16.274871470090183
20-24	0.2805329302034773	48.42411104287096	34.85683051781641	16.438525509109148
25-29	0.2923834708339322	48.45523391755331	34.700196364352806	16.552186247259947
30-34	0.27341875330511356	48.48719037213471	34.637443194243886	16.601947680316293
35-39	0.2794051707552435	48.48559171419354	34.602677625383656	16.632325489667565
40-44	0.2758899084426634	48.50259253275446	34.57051229670735	16.65100526209552
45-49	0.2774165622953835	48.49768753901655	34.559144057391954	16.66575184129611
50-54	0.2795454138894955	48.49386760850044	34.55178059890397	16.67480637870609
55-59	0.2825940000064811	48.486825932930834	34.544319274235704	16.686260792826978
60-64	0.2860836344548687	48.45937450300862	34.532145571116565	16.722396291419948
65-69	0.2895007637133643	48.44757159418882	34.525979552133954	16.736948089963864
70-74	0.29694760708730433	48.43028735905888	34.51835339981333	16.75441163404049
75-79	0.3012819782539964	48.413148652986074	34.52224716816531	16.763322200594626
80-84	0.30530146817459936	48.39258885985689	34.52479176223477	16.77731790973374
85-89	0.3102962678411919	48.37128623966893	34.52722192938462	16.79119556310526
90-94	0.32240890653808557	48.34407307192307	34.51383606709407	16.819681954444775
95-99	0.32884340762931147	48.323138345871186	34.50133563959	16.8466826069095
100-104	0.3365077286566359	48.30985227696403	34.47960158973003	16.874038404649305
105-109	0.34906849835060033	48.26377054286158	34.478309999970264	16.908850958817563
110-114	0.3517939206395382	48.242253417068454	34.47693918063147	16.929013481660533
115-119	0.371102729631182	48.21712922522333	34.45396537307208	16.957802672073402
120-124	0.37960269602751046	48.18663953317714	34.44423158937125	16.989526181424093
125-129	0.3975635793744154	48.165676818380874	34.42336433560591	17.0133952666388
130-134	0.40644979267934567	48.145844209201165	34.40731477941732	17.04039121870217
135-139	0.4284784587411844	48.110912968505396	34.38370649968437	17.076902073069057
140-144	0.4586292455224797	48.060320477489796	34.370949919090464	17.11010035789726
145-149	0.4754102167817805	48.00612356013017	34.348216205332285	17.17025001775577
150	0.48875047452624565	47.98560377982031	34.323978954356456	17.201666791296994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4469.0
1	5465.5
2	9276.5
3	20009.0
4	47639.5
5	112572.5
6	245283.0
7	484724.0
8	869354.5
9	1418932.5
10	2118906.5
11	2918476.0
12	3729618.5
13	4451079.5
14	4996447.0
15	5306903.5
16	5364243.0
17	5185773.0
18	4812609.5
19	4303696.0
20	3728734.5
21	3144336.5
22	2592981.0
23	2099554.0
24	1675825.0
25	1326315.5
26	1043191.5
27	816460.5
28	636201.0
29	495533.5
30	389254.0
31	306861.0
32	239995.0
33	186703.5
34	145595.0
35	114619.5
36	91156.5
37	73393.5
38	59846.5
39	49068.5
40	40705.5
41	34184.5
42	28912.0
43	24754.0
44	21132.5
45	18137.5
46	15733.5
47	13889.5
48	12513.5
49	11832.0
50	11262.5
51	10492.5
52	9587.5
53	8383.5
54	7443.5
55	6770.0
56	6099.5
57	5512.0
58	5080.5
59	4693.5
60	4329.0
61	3988.0
62	3674.5
63	3454.5
64	3161.5
65	2724.5
66	2463.0
67	2244.5
68	2019.0
69	1889.0
70	1744.0
71	1626.5
72	1524.5
73	1390.0
74	1313.0
75	1254.0
76	1127.5
77	1019.0
78	967.0
79	898.5
80	833.0
81	783.0
82	753.5
83	697.5
84	634.0
85	607.0
86	577.5
87	529.5
88	510.5
89	532.5
90	546.5
91	561.0
92	546.5
93	518.0
94	539.5
95	554.5
96	560.5
97	614.0
98	734.0
99	1179.5
100	2735.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.963798931898483
2	0.0016551394740715506
3	0.0017566910718396767
4	0.004283355452130221
5	1.470224624404216E-4
6	1.6672650379841626E-5
7	2.8798214292453714E-5
8	0.0015975430454866432
9	0.001858242669607803
10-14	4.989669550039876E-4
15-19	9.382155077383605E-4
20-24	0.0012340792672060957
25-29	4.3227635348462107E-4
30-34	7.830082896569257E-4
35-39	7.757329513093587E-4
40-44	0.0030707990608690124
45-49	2.2917315794836855E-4
50-54	0.0021456185343367096
55-59	3.7649875948660545E-4
60-64	8.657652633605033E-4
65-69	0.00415179308367838
70-74	0.0026964222750671136
75-79	0.0035925014482091474
80-84	0.001018850507757231
85-89	0.002362666128372466
90-94	0.002025878590699665
95-99	9.93689962638561E-4
100-104	0.0017982211282403732
105-109	0.0023990428201103023
110-114	4.559212031142146E-4
115-119	0.0028992223315055512
120-124	4.2439473694142317E-4
125-129	0.00281798105329105
130-134	0.001114036184471236
135-139	0.002436935207337215
140-144	0.0039877948317603015
145-149	8.712217671211788E-4
150	0.01195277462685737
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	6.5976313E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.52814737751727
#Duplication Level	Percentage of total
1	77.39021095646311
2	14.702718210712561
3	3.3345516488049176
4	1.1358362400390332
5	0.6516075814082698
6	0.43143088071890656
7	0.339413699037174
8	0.23919902924783454
9	0.18709625034074914
>10	1.3701705293612738
>50	0.08031708725395258
>100	0.10637001153465538
>500	0.0048483020577267735
>1k	0.01702704988192127
>5k	0.009202523137965695
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.121973684707116E-5	0.0	0.0	0.0	0.1849982129192336	0.0
2	2.4251127825224183E-5	0.0	0.0	0.0	0.23666827214185188	6.062781956306046E-6
3	2.7282518803377206E-5	0.0	0.0	0.0	0.2720233851200506	1.3641259401688603E-5
4	3.031390978153023E-5	0.0	0.0	0.0	0.31310934274244756	2.121973684707116E-5
5	3.182960527060674E-5	0.0	0.0	0.0	0.35525325581621997	2.5766823314300694E-5
6	3.4860996248759766E-5	0.0	0.0	0.0	0.3978033752810649	3.182960527060674E-5
7	3.94080827159893E-5	0.0	0.0	0.0	0.44018222115564415	3.334530075968325E-5
8	4.243947369414232E-5	0.0	0.0	1.5156954890765114E-6	0.479976806221348	5.153364662860139E-5
9	4.698656016137186E-5	0.0	0.0	3.031390978153023E-6	0.5219964322650161	7.123768798659603E-5
10-11	5.001795113952488E-5	0.0	0.0	3.031390978153023E-6	0.5852221538963537	8.033186092105511E-5
12-13	6.365921054121349E-5	0.0	0.0	3.031390978153023E-6	0.6671993022708014	9.24574248336672E-5
14-15	8.336325189920812E-5	0.0	0.0	3.031390978153023E-6	0.7471575442537991	1.0988792295804708E-4
16-17	9.094172934459069E-5	0.0	0.0	3.031390978153023E-6	0.8282328538122462	1.2277133461519743E-4
18-19	1.0382514100174103E-4	0.0	0.0	3.031390978153023E-6	0.9078803782199834	1.3565474627234776E-4
20-21	1.318655075496565E-4	0.0	0.0	3.031390978153023E-6	0.9881493377782418	1.4853815792949813E-4
22-23	1.727892857547223E-4	0.0	0.0	3.7892387226912786E-6	1.0683190192819656	1.5535878763034243E-4
24-25	2.3190140982870625E-4	0.0	0.0	6.062781956306046E-6	1.1487107501748393	1.5839017860849546E-4
26-27	2.887399906690754E-4	0.0	0.0	6.062781956306046E-6	1.228093027871988	1.6066372184211022E-4
28-29	3.5997767865567147E-4	0.0	0.0	6.062781956306046E-6	1.3079391083887941	1.6369511282026325E-4
30-31	4.463723215330326E-4	0.0	0.0	6.062781956306046E-6	1.3881557764526793	1.674843515429545E-4
32-33	5.418611373448529E-4	0.0	0.0	6.062781956306046E-6	1.4675994398171355	1.7354713349926055E-4
34-35	6.661481674491268E-4	0.0	0.0	6.062781956306046E-6	1.5474288476835618	1.7657852447741358E-4
36-37	7.61636983260947E-4	0.0	0.0	6.062781956306046E-6	1.6279433499110505	1.8339915417825789E-4
38-39	8.647042765181497E-4	0.0	0.0	6.062781956306046E-6	1.7084184440558234	1.849148496673344E-4
40-41	9.708029607535056E-4	0.0	0.0	6.820629700844301E-6	1.7889397669130132	1.947668703463317E-4
42-43	0.0010799330359670145	0.0	0.0	7.578477445382557E-6	1.868988192777611	2.01587500047176E-4
44-45	0.001209525000283056	0.0	0.0	7.578477445382557E-6	1.94910861417794	2.106816729816351E-4
46-47	0.0013557896149789393	0.0	0.0	7.578477445382557E-6	2.029132031066968	2.175023026824794E-4
48-49	0.0015247896620109705	0.0	0.0	9.094172934459069E-6	2.1091668156721637	2.2204938914970892E-4
50-51	0.001698336795510231	0.0	0.0	9.094172934459069E-6	2.1892197886232294	2.3417495306232104E-4
52-53	0.0018673368425422621	0.0	0.0	9.094172934459069E-6	2.2687468152395844	2.379641917850123E-4
54-55	0.002021179934683528	0.0	0.0	9.094172934459069E-6	2.348614873340982	2.4933190795308613E-4
56-57	0.002162897462912182	0.0	0.0	9.094172934459069E-6	2.4279994245813645	2.576682331430069E-4
58-59	0.0023228033370097535	0.0	0.0	9.094172934459069E-6	2.507409742645061	2.6979379705561904E-4
60-61	0.0025001397092317057	0.0	0.0	9.094172934459069E-6	2.5865434462820014	2.788879699900781E-4
62-63	0.002682023167920887	0.0	0.0	9.094172934459069E-6	2.665899957155836	2.879821429245372E-4
64-65	0.002851781062697456	0.0	0.0	9.094172934459069E-6	2.745054122681878	2.925292293917667E-4
66-67	0.0030723147563580886	0.0	0.0	9.094172934459069E-6	2.8241059787624083	3.001077068371493E-4
68-69	0.0033133103391212544	0.0	0.0	9.094172934459069E-6	2.9033760949933654	3.1223327074976135E-4
70-71	0.0035891669181331793	0.0	0.0	9.094172934459069E-6	2.982415067662238	3.220852914287587E-4
72-73	0.0039044315798610935	0.0	0.0	1.060986842353558E-5	3.060318026562048	3.296637688741412E-4
74-75	0.004219696241589008	0.0	0.0	1.060986842353558E-5	3.139294855715869	3.4330502827582983E-4
76-77	0.004571337595054759	0.0	0.0	1.060986842353558E-5	3.2185937701611183	3.6755615610105407E-4
78-79	0.00496162918349196	0.0	0.0	1.060986842353558E-5	3.2973121732340513	3.880180452035869E-4
80-81	0.00536556203133085	0.0	0.0	1.060986842353558E-5	3.3758615762599526	4.054485433279668E-4
82-83	0.005855889522047102	0.0	0.0	1.060986842353558E-5	3.453872149539487	4.3348890987588226E-4
84-85	0.006393961420669263	0.0	0.0	1.060986842353558E-5	3.531624296738134	4.6001358093472123E-4
86-87	0.006997208225321715	0.0	0.0	1.2125563912612091E-5	3.6089603855250294	4.842647087599454E-4
88-89	0.007626979701033005	0.0	0.0	1.2125563912612091E-5	3.6859145190486773	5.085158365851696E-4
90-91	0.008318894691796433	0.0	0.0	1.2125563912612091E-5	3.762734513521542	5.24430639220473E-4
92-93	0.009091899391225454	0.0	0.0	1.2125563912612091E-5	3.8401425978441686	5.441346805784677E-4
94-95	0.009943720256086454	0.0	0.0	1.2125563912612091E-5	3.9173778928810403	5.66112265170077E-4
96-97	0.010921343846540803	0.0	0.0	1.2125563912612091E-5	3.994456313434793	5.92636936228916E-4
98-99	0.011981572841149823	0.0	0.0	1.2125563912612091E-5	4.071768151093863	6.191616072877549E-4
100-101	0.013266124768142167	0.0	0.0	1.2125563912612091E-5	4.149034517888261	6.396234963902878E-4
102-103	0.014684057898173243	0.0	0.0	1.2125563912612091E-5	4.226405467671405	6.706952539163563E-4
104-105	0.016056520163532026	0.0	0.0	1.2125563912612091E-5	4.303283058572855	7.010091636978865E-4
106-107	0.017649516122551438	0.0	0.0	1.2125563912612091E-5	4.3801227570870775	7.343544644575698E-4
108-109	0.019414543519581037	0.0	0.0	1.2125563912612091E-5	4.456640370309872	7.798253291298651E-4
110-111	0.021339476790708203	0.0	0.0	1.2883411657150347E-5	4.532356938466689	8.146863253786249E-4
112-113	0.02346220832315986	0.0	0.0	1.3641259401688603E-5	4.6085774753736235	8.533365603500759E-4
114-115	0.025745603577453625	0.0	0.0	1.3641259401688603E-5	4.684663873229775	8.935024908106035E-4
116-117	0.02825862669834248	0.0	0.0	1.3641259401688603E-5	4.7596301721195	9.48067528417358E-4
118-119	0.031106618522317244	0.0	0.0	1.5156954890765114E-5	4.835175466686051	9.935383930896532E-4
120-121	0.03441613962271581	0.0	0.0	1.5156954890765114E-5	4.911267169476415	0.0010299150848274894
122-123	0.03790905987729262	0.0	0.0	1.5156954890765114E-5	4.9869746434602975	0.0010799330359670145
124-125	0.04180363943647472	0.0	0.0	1.5156954890765114E-5	5.062392619605767	0.0011216146619166185
126-127	0.046033187698742734	0.0	0.0	1.6672650379841626E-5	5.138601788796533	0.0011663276788443757
128-129	0.0508167226622682	0.0	0.0	1.6672650379841626E-5	5.213677217761472	0.001215587782239362
130-131	0.05608982726876538	0.0	0.0	1.6672650379841626E-5	5.288964086247136	0.0012671214288679637
132-133	0.06202377510849992	0.0	0.0	1.8188345868918137E-5	5.364325981659508	0.0013262335529419475
134-135	0.06837453920773051	0.0	0.0	1.8188345868918137E-5	5.438548831911842	0.0013861035247604697
136-137	0.07552028559098173	0.0	0.0	1.8188345868918137E-5	5.513240032070298	0.0014656775379369865
138-139	0.0834973909499914	0.0	0.0	2.1977584591609417E-5	5.588241191956271	0.001535399530434506
>>END_MODULE
