##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065368_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65976313
Total Bases	9.8 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17085045355596	32.0	32.0	32.0	32.0	32.0
2	31.41707450369347	32.0	32.0	32.0	32.0	32.0
3	34.896632235269045	37.0	32.0	37.0	32.0	37.0
4	35.72844341877668	37.0	37.0	37.0	32.0	37.0
5	36.13745258847671	37.0	37.0	37.0	37.0	37.0
6	39.63154606714686	41.0	41.0	41.0	37.0	41.0
7	39.80979599451094	41.0	41.0	41.0	37.0	41.0
8	39.87235676537426	41.0	41.0	41.0	37.0	41.0
9	39.917690095837884	41.0	41.0	41.0	37.0	41.0
10-14	39.94682704382102	41.0	41.0	41.0	37.0	41.0
15-19	39.930024940920845	41.0	41.0	41.0	37.0	41.0
20-24	39.88343820910392	41.0	41.0	41.0	37.0	41.0
25-29	39.75036396168424	41.0	41.0	41.0	37.0	41.0
30-34	39.64399715697966	41.0	41.0	41.0	37.0	41.0
35-39	39.591392798806446	41.0	41.0	41.0	37.0	41.0
40-44	39.51233591061689	41.0	41.0	41.0	37.0	41.0
45-49	39.455841656383555	41.0	41.0	41.0	37.0	41.0
50-54	39.372021507173336	41.0	41.0	41.0	37.0	41.0
55-59	39.28720417280668	41.0	41.0	41.0	37.0	41.0
60-64	39.182403578690426	41.0	41.0	41.0	37.0	41.0
65-69	39.06199133619364	41.0	41.0	41.0	37.0	41.0
70-74	38.975445466314554	41.0	41.0	41.0	37.0	41.0
75-79	38.800556269338664	41.0	40.2	41.0	36.0	41.0
80-84	39.05076413409158	41.0	41.0	41.0	37.0	41.0
85-89	38.95681657142617	41.0	41.0	41.0	37.0	41.0
90-94	38.84852713427621	41.0	41.0	41.0	37.0	41.0
95-99	38.71745249541301	41.0	41.0	41.0	33.0	41.0
100-104	38.57700679636342	41.0	41.0	41.0	32.0	41.0
105-109	38.44550667146253	41.0	41.0	41.0	32.0	41.0
110-114	38.290442195519475	41.0	37.8	41.0	32.0	41.0
115-119	38.12532704578384	41.0	37.0	41.0	32.0	41.0
120-124	37.884465905210554	41.0	37.0	41.0	32.0	41.0
125-129	37.68444745616506	41.0	37.0	41.0	29.0	41.0
130-134	37.435687586846505	41.0	37.0	41.0	27.0	41.0
135-139	37.153596148969406	41.0	37.0	41.0	27.0	41.0
140-144	36.903610651901694	41.0	37.0	41.0	27.0	41.0
145-149	36.62711078747308	41.0	37.0	41.0	27.0	41.0
150	36.45632954663593	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	11.0
13	8.0
14	19.0
15	53.0
16	292.0
17	995.0
18	2249.0
19	3937.0
20	6895.0
21	12037.0
22	20677.0
23	35797.0
24	61783.0
25	100143.0
26	156512.0
27	235754.0
28	342304.0
29	475989.0
30	643363.0
31	848673.0
32	1095368.0
33	1401170.0
34	1785936.0
35	2295844.0
36	3038786.0
37	4255278.0
38	6726436.0
39	1.3624259E7
40	2.8805745E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.019480164573277	41.14943260554671	42.415135545410116	0.4159516844698978
2	17.44987131910153	40.16310786635617	42.14663195807953	0.2403888564627616
3	14.68518644527628	36.40077726730362	48.611241702752785	0.3027945846673197
4	15.563969009132276	35.438403550215476	48.68027968427297	0.317347756379284
5	16.299339127968548	35.4727658091473	47.91629383715335	0.31160122573081644
6	16.303052136405753	35.143969473391635	48.230368496992206	0.3226098932104051
7	16.71353122044441	35.54085472173244	47.450427769497786	0.2951862883253704
8	15.58230431863708	34.69542192127443	49.417659835158084	0.30461392493040795
9	16.02952562687157	34.200278818247995	49.46705494136964	0.30314061351079136
10-14	16.120810937671735	35.09226560445348	48.501540848315656	0.2853826095591274
15-19	16.101447812425924	34.9232912696882	48.69201441665049	0.2832465012353913
20-24	16.267091347394608	34.84567437664075	48.60018512828297	0.2870491476816648
25-29	16.39538415164527	34.73985504686134	48.57062476522496	0.29413603626843227
30-34	16.51377199714134	34.62344049349898	48.566276480295386	0.2965110290642904
35-39	16.564358822180218	34.58195597554739	48.557059093942634	0.29662610832976116
40-44	16.580387496956238	34.57029190891393	48.549978300261145	0.29934229386868383
45-49	16.602070903615555	34.54422508791593	48.55245803030091	0.30124597816760285
50-54	16.608150873595694	34.53105126590603	48.55815039069308	0.3026474698052027
55-59	16.627376552056226	34.5397413338114	48.5276102265423	0.30527188759007284
60-64	16.628822184387374	34.5192175825423	48.54428568951466	0.3076745435556623
65-69	16.649342476628686	34.518780187537054	48.52063455875013	0.31124277708413384
70-74	16.648302683008147	34.5216572195289	48.515058238234964	0.31498185922799726
75-79	16.66414115597475	34.503144813284266	48.52548305564311	0.30723097509787445
80-84	16.663619168252712	34.49110565776735	48.53702584768534	0.3082493262945912
85-89	16.685948353707516	34.488594554418206	48.501552881219574	0.32390421065470143
90-94	16.672912783255285	34.48171926562936	48.52357755117688	0.3217903999384794
95-99	16.68977416691332	34.46805995441105	48.51426709739634	0.32789878127928584
100-104	16.67219527841854	34.47411443348721	48.52520208004336	0.32848820805089857
105-109	16.68244414201248	34.460196947793406	48.519710701767536	0.3376482084265763
110-114	16.69193920708933	34.44845502756545	48.52144664876462	0.3381591165805997
115-119	16.684447792357986	34.43618994839065	48.53116719937391	0.34819505987745497
120-124	16.684398656727296	34.436294975059674	48.520807905049665	0.3584984631633636
125-129	16.688997076293038	34.438446221343014	48.50354768997721	0.3690090123867377
130-134	16.689315369740935	34.42852726520275	48.497645561519086	0.3845118035372234
135-139	16.704505962802052	34.39939406602179	48.494335919102355	0.4017640520738017
140-144	16.694217579258343	34.39879908462623	48.49632985176218	0.4106534843532403
145-149	16.693025045707017	34.378767055734166	48.50284104916614	0.42536684939267766
150	16.67987365431361	34.39851561522796	48.48885868647512	0.432752043983316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4933.0
1	6481.5
2	11392.0
3	23152.5
4	52133.5
5	117878.5
6	250937.0
7	491179.5
8	874045.5
9	1417300.0
10	2110529.5
11	2902676.0
12	3705657.5
13	4422169.5
14	4966993.5
15	5285440.0
16	5353484.5
17	5184585.5
18	4825583.0
19	4330605.0
20	3761568.0
21	3175903.0
22	2622531.5
23	2127033.5
24	1700487.5
25	1348374.5
26	1063619.5
27	833238.5
28	648608.5
29	507931.0
30	399870.0
31	314438.0
32	244879.0
33	187907.5
34	144487.5
35	112128.5
36	87549.5
37	68573.0
38	53947.0
39	42775.0
40	34000.0
41	26900.0
42	21384.5
43	17101.0
44	13813.0
45	11065.5
46	9049.0
47	7569.5
48	6231.0
49	5776.0
50	6091.5
51	5906.0
52	4958.0
53	3937.0
54	3212.5
55	2746.0
56	2309.0
57	1919.5
58	1647.5
59	1495.5
60	1308.5
61	1113.0
62	892.5
63	664.5
64	520.0
65	391.0
66	324.0
67	282.5
68	228.5
69	209.5
70	186.0
71	135.5
72	109.0
73	100.5
74	80.0
75	61.0
76	58.0
77	56.0
78	55.5
79	52.0
80	41.5
81	39.0
82	39.0
83	30.5
84	27.0
85	23.5
86	17.0
87	16.0
88	15.0
89	13.5
90	9.5
91	7.0
92	8.5
93	5.5
94	5.0
95	8.0
96	7.0
97	12.0
98	19.5
99	48.5
100	739.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7760042608019033
2	0.0010746281017552466
3	0.007860396806350789
4	0.00876375131784039
5	0.0
6	5.3049342117677896E-5
7	1.5156954890765116E-5
8	1.8188345868918137E-5
9	0.0
10-14	3.843803760298033E-4
15-19	3.2011488729295924E-4
20-24	3.4012206774876915E-4
25-29	0.0013974712409285436
30-34	3.295121993252336E-4
35-39	9.042639287830468E-4
40-44	6.120378384890954E-4
45-49	0.0011016074814608086
50-54	4.216664850610855E-4
55-59	2.661561278818354E-4
60-64	0.0010443141919737164
65-69	0.0011243429137969563
70-74	1.0458298874627929E-4
75-79	3.3042161661867947E-4
80-84	0.0013474532897890187
85-89	0.0011492003198178109
90-94	5.847553196857181E-4
95-99	0.0018148937786202147
100-104	0.0020340633463406784
105-109	2.86769586533276E-4
110-114	1.4217223687537677E-4
115-119	5.495911843391431E-4
120-124	1.1337402258292306E-4
125-129	0.0014544613913178204
130-134	7.533006580710262E-4
135-139	0.0016254318424856508
140-144	9.700451130089674E-4
145-149	0.001840054323738885
150	0.005548961185509109
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	6.5976313E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.81625021483211
#Duplication Level	Percentage of total
1	76.20474080747314
2	15.465179640932533
3	3.539386840854307
4	1.19282516682969
5	0.6375204218167168
6	0.4454426555339389
7	0.3474214847251255
8	0.2592817284883971
9	0.19211315406231186
>10	1.4696392207814226
>50	0.10259102567877847
>100	0.10599802415048078
>500	0.009874086598966061
>1k	0.01889394749489178
>5k	0.00909179457937217
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.121973684707116E-5	0.0	0.0	0.0	0.002082565601991127	8.002872182323981E-4
2	2.4251127825224183E-5	0.0	0.0	0.0	0.008201428291393004	0.0036725301700323873
3	2.7282518803377206E-5	0.0	0.0	0.0	0.01346392302946665	0.0053989073320905336
4	3.182960527060674E-5	0.0	0.0	0.0	0.019144749722525417	0.007454190415278283
5	3.334530075968325E-5	0.0	0.0	0.0	0.024681585344121914	0.009841410810573789
6	3.6376691737836274E-5	0.0	0.0	0.0	0.03060947040190015	0.012539348781129979
7	3.94080827159893E-5	0.0	0.0	0.0	0.03621602801599416	0.015422201601353503
8	4.243947369414232E-5	0.0	0.0	0.0	0.04150277388189304	0.01842631006070315
9	4.547086467229534E-5	0.0	0.0	0.0	0.04678648835681375	0.021387979046358654
10-11	4.8502255650448366E-5	0.0	0.0	0.0	0.05474085828348729	0.026167724771161433
12-13	5.6838580840369183E-5	0.0	0.0	0.0	0.06461030946060899	0.032796618992637555
14-15	6.972199249751952E-5	0.0	0.0	0.0	0.07400913718837243	0.04012955376878972
16-17	7.502692670928732E-5	0.0	0.0	0.0	0.08319879893864332	0.047613300246104995
18-19	7.805831768744034E-5	0.0	0.0	0.0	0.09215277004036282	0.055172831497874095
20-21	8.866818611097591E-5	0.0	0.0	0.0	0.10059898315324167	0.06259670800337085
22-23	1.0761437972443231E-4	0.0	0.0	0.0	0.1089830527510684	0.07000618540172138
24-25	1.318655075496565E-4	0.0	0.0	0.0	0.11715340928493534	0.07762482877756446
26-27	1.538430921412659E-4	0.0	0.0	0.0	0.12515476577177023	0.08547006862902448
28-29	1.6217941733118673E-4	0.0	0.0	0.0	0.1331000415255093	0.09313115147856171
30-31	1.8339915417825789E-4	0.0	0.0	0.0	0.14095361770215925	0.10076646750478463
32-33	2.190179981715559E-4	0.0	0.0	0.0	0.14886857954611682	0.10859730824909844
34-35	2.576682331430069E-4	0.0	0.0	0.0	0.15692450107055844	0.1163068933542861
36-37	2.8116151322369286E-4	0.0	0.0	0.0	0.1649266154051379	0.12401041567751747
38-39	3.114754230052231E-4	0.0	0.0	0.0	0.17286810192015428	0.13180260618685982
40-41	3.3951578955313856E-4	0.0	0.0	0.0	0.18098768265513715	0.1396887698165249
42-43	3.6907185159013056E-4	0.0	0.0	0.0	0.1892376132021806	0.14734985266606215
44-45	4.039328478388903E-4	0.0	0.0	0.0	0.1972564001871399	0.15524359477317262
46-47	4.4485662604395613E-4	0.0	0.0	0.0	0.20559727246352794	0.1632426777167739
48-49	4.979059681616341E-4	0.0	0.0	0.0	0.21389570526622184	0.17127434811339032
50-51	5.494396147902354E-4	0.0	0.0	0.0	0.22227977486404854	0.17924387499495464
52-53	6.009732614188368E-4	0.0	0.0	0.0	0.23060018525133408	0.1874051373558871
54-55	6.449284306020557E-4	0.0	0.0	0.0	0.23894939385291203	0.19563687955706166
56-57	6.911571430188892E-4	0.0	0.0	0.0	0.24729329752027823	0.203667034258189
58-59	7.495114193483349E-4	0.0	0.0	0.0	0.2558016541482092	0.21184269572626768
60-61	8.177177163567779E-4	0.0	0.0	0.0	0.2643183471013302	0.21989634370747574
62-63	9.010809682559861E-4	0.0	0.0	0.0	0.27265846152997364	0.22822736396318477
64-65	9.685294175198908E-4	0.0	0.0	0.0	0.2811418091823349	0.23651291335422153
66-67	0.0010602289946090196	0.0	0.0	0.0	0.28950693258654814	0.24508720273592738
68-69	0.0011867895679469084	0.0	0.0	0.0	0.2981335134626271	0.25368195400673577
70-71	0.0013300227916646387	0.0	0.0	0.0	0.3068995383237011	0.2622744317343104
72-73	0.0015103905548647435	0.0	0.0	0.0	0.315782271737434	0.2711897526010585
74-75	0.001692274013553925	0.0	0.0	0.0	0.3243285207525919	0.28007475955802497
76-77	0.0019006821433019453	0.0	0.0	0.0	0.33303543348959197	0.28913634503946894
78-79	0.002131825705386113	0.0	0.0	0.0	0.3419598485292744	0.2978932757276085
80-81	0.002419050000566112	0.0	0.0	0.0	0.3508236357493939	0.3064198207014084
82-83	0.002760081485608327	0.0	0.0	0.0	0.3597154633360612	0.3152411684478337
84-85	0.003165530028936294	0.0	0.0	0.0	0.3686845913926715	0.32409283010404055
86-87	0.0036119023504693265	0.0	0.0	0.0	0.3776097642800985	0.3327504827376455
88-89	0.004088588581783889	0.0	0.0	0.0	0.3868320134833846	0.341535453792333
90-91	0.004631207566873281	0.0	0.0	0.0	0.3960034868877865	0.35046820515720545
92-93	0.005245822087693806	0.0	0.0	0.0	0.4051143324726254	0.3594153556292241
94-95	0.005940768469435386	0.0	0.0	0.0	0.4141099852002945	0.3684398065711856
96-97	0.006790315791062771	0.0	0.0	0.0	0.42333071870809147	0.3775309481146666
98-99	0.0077080694096985985	0.0	0.0	0.0	0.4324953714827926	0.386375031293428
100-101	0.008841051787783293	0.0	0.0	0.0	0.4419798360056889	0.39526306964137264
102-103	0.010081648545592415	0.0	0.0	1.5156954890765114E-6	0.4514248924458692	0.4042602380645308
104-105	0.011300267718809931	0.0	0.0	1.5156954890765114E-6	0.460583482438614	0.41334759036928903
106-107	0.012683339852592249	0.0	0.0	1.5156954890765114E-6	0.47014676312694226	0.42248950771165406
108-109	0.014286945680035197	0.0	0.0	1.5156954890765114E-6	0.47945464912536107	0.4319338063040898
110-111	0.016026206253750494	0.0	0.0	1.5156954890765114E-6	0.4887989118155178	0.4411401407047405
112-113	0.017967812175257503	0.0	0.0	1.5156954890765114E-6	0.49819622384779216	0.4502487127463458
114-115	0.020130709638169687	0.0	0.0	1.5156954890765114E-6	0.507564737665774	0.45938153591577635
116-117	0.022465638539092052	0.0	0.0	1.5156954890765114E-6	0.5171484802432049	0.46848480302316986
118-119	0.02512265273144318	0.0	0.0	1.5156954890765114E-6	0.5269671556214425	0.47748576068505066
120-121	0.02823664911375087	0.0	0.0	1.5156954890765114E-6	0.5367085305297372	0.4865905434879333
122-123	0.03152191908632421	0.0	0.0	1.5156954890765114E-6	0.5463043986710807	0.4960969855954212
124-125	0.035169440280786834	7.578477445382557E-7	0.0	1.5156954890765114E-6	0.5557615806751736	0.5054579209359578
126-127	0.03919285395654043	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.5655370587319725	0.51448919250762
128-129	0.0437247834688792	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.575392110801948	0.5235136434495817
130-131	0.048727336430576224	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.5852949072798294	0.53268284331075
132-133	0.054393764016488766	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.595054470533993	0.5420066441118042
134-135	0.06054066707243856	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.6049405943614945	0.5512917946778869
136-137	0.0674590591323283	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.6149206913093188	0.5606231739563865
138-139	0.07515955006458151	1.5156954890765114E-6	0.0	1.5156954890765114E-6	0.6248659272608944	0.5699181765431481
>>END_MODULE
