##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065367_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74770544
Total Bases	11.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.84687777582573	32.0	32.0	32.0	32.0	32.0
2	31.228135413860304	32.0	32.0	32.0	32.0	32.0
3	34.87625003771539	37.0	32.0	37.0	32.0	37.0
4	35.63812118312259	37.0	37.0	37.0	32.0	37.0
5	35.8415436298016	37.0	37.0	37.0	37.0	37.0
6	39.26686926338265	41.0	41.0	41.0	37.0	41.0
7	39.38138220580554	41.0	41.0	41.0	37.0	41.0
8	39.52094712591632	41.0	41.0	41.0	37.0	41.0
9	39.56151320766103	41.0	41.0	41.0	37.0	41.0
10-14	39.55571584713895	41.0	41.0	41.0	37.0	41.0
15-19	39.51833992273748	41.0	41.0	41.0	37.0	41.0
20-24	39.454473261556046	41.0	41.0	41.0	37.0	41.0
25-29	39.40013105963226	41.0	41.0	41.0	37.0	41.0
30-34	39.33870329470922	41.0	41.0	41.0	37.0	41.0
35-39	39.258628609148545	41.0	41.0	41.0	37.0	41.0
40-44	39.18523087380506	41.0	41.0	41.0	37.0	41.0
45-49	39.091575853721224	41.0	41.0	41.0	37.0	41.0
50-54	38.967878032290365	41.0	41.0	41.0	37.0	41.0
55-59	38.85424971898024	41.0	41.0	41.0	37.0	41.0
60-64	38.75320320793707	41.0	41.0	41.0	36.0	41.0
65-69	38.65643246623965	41.0	41.0	41.0	32.0	41.0
70-74	38.54003786838838	41.0	41.0	41.0	32.0	41.0
75-79	38.32014559637282	41.0	39.4	41.0	32.0	41.0
80-84	38.505111956922505	41.0	41.0	41.0	32.0	41.0
85-89	38.382517709112825	41.0	41.0	41.0	32.0	41.0
90-94	38.21057486488262	41.0	41.0	41.0	32.0	41.0
95-99	38.01811634003893	41.0	38.6	41.0	31.0	41.0
100-104	37.81166404246035	41.0	37.0	41.0	28.0	41.0
105-109	37.57305577715202	41.0	37.0	41.0	27.0	41.0
110-114	37.3706540853842	41.0	37.0	41.0	27.0	41.0
115-119	37.11330080733397	41.0	37.0	41.0	27.0	41.0
120-124	36.77199793276882	41.0	37.0	41.0	26.0	41.0
125-129	36.41332725090244	41.0	37.0	41.0	22.0	41.0
130-134	36.055456512393434	41.0	37.0	41.0	22.0	41.0
135-139	35.61355238768893	41.0	33.0	41.0	22.0	41.0
140-144	35.05041652231392	39.4	32.0	41.0	22.0	41.0
145-149	34.48096706638914	37.0	32.0	41.0	16.0	41.0
150	34.10599889443094	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	61.0
12	691.0
13	2090.0
14	5474.0
15	12846.0
16	22898.0
17	33201.0
18	48080.0
19	73054.0
20	115508.0
21	184244.0
22	269453.0
23	358561.0
24	445347.0
25	537304.0
26	641271.0
27	755404.0
28	880700.0
29	1012667.0
30	1145797.0
31	1276487.0
32	1403368.0
33	1564897.0
34	1817066.0
35	2257525.0
36	3010963.0
37	4341653.0
38	6968611.0
39	1.3929909E7
40	3.1655414E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.11299109992175191	51.396582473671295	32.42686694071053	16.063559485696427
2	0.12465660317136178	52.79701480056749	33.90557422139645	13.172754374864695
3	0.19702517319782498	48.901915745277	34.776432989199314	16.12462609232586
4	0.24804290774106766	47.7286107445226	35.21936987196355	16.80397647577278
5	0.2663540260598541	48.10093527505151	35.13382987823886	16.49888082064976
6	0.27610029191539626	47.61608801484718	35.31019685438177	16.797614838855658
7	0.27976132839520174	48.28894061456307	34.849950221022944	16.581347836018796
8	0.25834179535798457	46.98140873401312	35.99341449966949	16.7668349709594
9	0.27026268302347384	47.34307809020719	35.840799945978226	16.545859280791113
10-14	0.27143052201799905	47.72363389902365	35.597605112415486	16.407330466542863
15-19	0.2866337440785595	47.36962810894015	35.80018710824252	16.543551038738777
20-24	0.31481255043916817	47.55562355904642	35.387188579751815	16.742375310762597
25-29	0.32002600003447357	47.53155509648834	35.289473199076	16.858945704401187
30-34	0.31017753366313155	47.67744311928676	35.13383375306981	16.878545593980302
35-39	0.31746640679223725	47.620991106051505	35.16484133170199	16.89670115545426
40-44	0.3146008052895965	47.67815285197499	35.10538955247199	16.901856790263427
45-49	0.3164460269408758	47.64046146242612	35.136124686839025	16.90696782379398
50-54	0.3176453674646645	47.66265447335328	35.128682697710644	16.891017461471414
55-59	0.32434890471211003	47.649189042410136	35.120387871197366	16.90607418168039
60-64	0.3275479066441455	47.62723868555941	35.12746237095412	16.917751036842326
65-69	0.33287269046810486	47.61724703211215	35.12801244520567	16.921867832214076
70-74	0.34116003393897765	47.58568741365096	35.14177455886689	16.93137799354317
75-79	0.3466441142655572	47.58897090731751	35.13469150823674	16.92969347018019
80-84	0.34946792599483234	47.573103351630365	35.144017061975916	16.933411660398885
85-89	0.35542455019327585	47.55476684252438	35.147081977263774	16.942726630018573
90-94	0.368193746640511	47.524503306932424	35.147818692167746	16.959484254259323
95-99	0.3776664146830918	47.50350865541807	35.13809199569269	16.98073293420616
100-104	0.38388051392804257	47.47396904052364	35.13604894839531	17.006101497152994
105-109	0.39376020431677994	47.44609321436825	35.122729992998934	17.03741658831604
110-114	0.3967543274069338	47.4264574155565	35.136037508404094	17.040750748632476
115-119	0.42039296388260877	47.39822480121879	35.11283144476305	17.068550790135543
120-124	0.4281890086245959	47.36423027291594	35.1106735116934	17.096907206766062
125-129	0.44705291874713143	47.348907799399385	35.0917094526612	17.112329829192284
130-134	0.455925302439415	47.32386511886256	35.08134715723728	17.13886242146075
135-139	0.4820654407196795	47.28619415826813	35.05556555464654	17.176174846365644
140-144	0.5070090955676174	47.23673415721664	35.04546526581169	17.21079148140405
145-149	0.5210697121091244	47.17326829803269	35.02388736606364	17.281774623794547
150	0.5357352929275919	47.13848334311664	35.005585410771964	17.320195953183802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5084.0
1	6397.0
2	11716.5
3	25952.0
4	60054.5
5	135383.0
6	284072.5
7	545727.5
8	964641.0
9	1561729.5
10	2322899.0
11	3196693.0
12	4086589.0
13	4884517.0
14	5493144.0
15	5855356.5
16	5949270.0
17	5783966.5
18	5399600.5
19	4864120.5
20	4250298.0
21	3613134.5
22	3007012.5
23	2463673.5
24	1994162.0
25	1598339.0
26	1273111.0
27	1009135.0
28	795876.0
29	631255.5
30	503747.5
31	401120.5
32	316845.5
33	248203.5
34	195635.0
35	155566.0
36	125063.0
37	101328.0
38	82538.5
39	68133.5
40	56822.0
41	47593.5
42	40348.5
43	34631.5
44	29714.0
45	25627.0
46	22403.0
47	19841.0
48	17621.0
49	16705.0
50	16063.5
51	15081.5
52	13787.0
53	11929.5
54	10608.0
55	9705.0
56	8837.5
57	8007.5
58	7330.5
59	6656.0
60	6099.0
61	5715.0
62	5404.0
63	5074.0
64	4597.5
65	4184.5
66	3797.5
67	3426.5
68	3035.0
69	2689.0
70	2485.5
71	2230.0
72	2061.0
73	1915.5
74	1774.5
75	1652.0
76	1509.0
77	1432.5
78	1332.0
79	1173.5
80	1118.5
81	1074.0
82	1001.5
83	953.0
84	858.0
85	779.0
86	760.5
87	712.5
88	653.5
89	637.0
90	649.0
91	647.5
92	627.0
93	595.5
94	586.5
95	623.5
96	635.5
97	671.5
98	788.5
99	1252.0
100	3145.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9683010999625735
2	0.004742509296174173
3	0.0018964687484419
4	0.0010953511318574866
5	1.7787753423326703E-4
6	3.3435626735576514E-4
7	3.209820166615345E-5
8	2.928960902036503E-4
9	9.29510423249027E-4
10-14	6.296597226843769E-4
15-19	7.510979189879908E-4
20-24	7.037530715304145E-4
25-29	2.4287639260722779E-4
30-34	1.8509962960815157E-4
35-39	2.3565429723234325E-4
40-44	0.0026925041497625054
45-49	1.7573765412219014E-4
50-54	0.002092267778605436
55-59	4.2904596227091786E-4
60-64	4.7023865440914807E-4
65-69	0.003825570668577722
70-74	0.0023573454273650863
75-79	0.0031212826270195382
80-84	4.0925207124345653E-4
85-89	0.0023592178224622787
90-94	8.235863577507206E-4
95-99	7.906857010429133E-4
100-104	5.395172730052626E-4
105-109	0.0025972794848195834
110-114	1.917867549552669E-4
115-119	0.0030878470002839624
120-124	5.157111067695321E-4
125-129	0.0027221949863036974
130-134	5.951541558932619E-4
135-139	0.002039840715884052
140-144	0.0038140688129806835
145-149	0.001195658012064216
150	0.01178137743654774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.4770544E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.68035014136554
#Duplication Level	Percentage of total
1	76.14583396974804
2	15.263723458812795
3	3.5574232135644346
4	1.245426121965306
5	0.6562398772622317
6	0.40105594202119027
7	0.3451651755733067
8	0.24902542844434514
9	0.23514257906234343
>10	1.582773245560175
>50	0.12167285717123162
>100	0.14307132613627196
>500	0.024872793086057804
>1k	0.020550151662476826
>5k	0.008023859929801924
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.541107631903815E-5	0.0	0.0	0.0	0.16336780965509626	1.3374250694230604E-6
2	2.541107631903815E-5	0.0	0.0	0.0	0.21015762570886204	4.0122752082691815E-6
3	2.541107631903815E-5	0.0	0.0	0.0	0.24533992958510506	6.687125347115303E-6
4	2.541107631903815E-5	0.0	0.0	0.0	0.2831475988726256	8.024550416538363E-6
5	2.674850138846121E-5	0.0	0.0	0.0	0.32233950310699894	9.361975485961424E-6
6	2.674850138846121E-5	0.0	0.0	0.0	0.3612545603520017	1.3374250694230606E-5
7	3.209820166615345E-5	0.0	0.0	0.0	0.40030068525380796	1.4711675763653665E-5
8	4.012275208269181E-5	0.0	0.0	0.0	0.43739951925453424	2.1398801110768967E-5
9	4.4135027290961E-5	0.0	0.0	1.3374250694230604E-6	0.4762998648237734	2.808592645788427E-5
10-11	4.4135027290961E-5	0.0	0.0	2.0061376041345908E-6	0.5328903317862713	3.076077659673039E-5
12-13	4.680987742980712E-5	0.0	0.0	3.343562673557651E-6	0.6065135757204068	3.343562673557652E-5
14-15	5.149086517278783E-5	0.0	0.0	5.349700277692242E-6	0.678783345484286	4.0791464617403344E-5
16-17	6.553382840172997E-5	0.0	0.0	6.687125347115303E-6	0.7514229132798607	4.814730249923018E-5
18-19	8.158292923480669E-5	0.0	0.0	8.693262951249894E-6	0.822994547157501	5.550314038105701E-5
20-21	1.2304310638692155E-4	0.0	0.0	9.361975485961424E-6	0.8961570481552201	5.684056545048007E-5
22-23	1.6784684621259412E-4	0.0	0.0	9.361975485961424E-6	0.9684843271970844	5.884670305461466E-5
24-25	2.4408007516970855E-4	0.0	0.0	9.361975485961424E-6	1.0405608390384322	6.553382840172997E-5
26-27	3.296752796127844E-4	0.0	0.0	1.0030688020672953E-5	1.1125537618129406	7.021481614471068E-5
28-29	4.092520712434565E-4	0.0	0.0	1.1368113090096014E-5	1.1855450724017735	7.28896662835568E-5
30-31	5.242706272138398E-4	0.0	0.0	1.4711675763653665E-5	1.2585858677181752	7.422709135297986E-5
32-33	6.700499597809533E-4	0.0	0.0	1.4711675763653665E-5	1.3316647796490555	7.757065402653751E-5
34-35	8.037924667232594E-4	0.0	0.0	1.6049100833076726E-5	1.404232795203416	8.225164176951822E-5
36-37	9.415472488738346E-4	0.0	0.0	1.8055238437211316E-5	1.4771044062485355	9.228232979019118E-5
38-39	0.0011080566700170057	0.0	0.0	1.8723950971922848E-5	1.5501585758156313	1.0097559274144107E-4
40-41	0.0012431366020287347	0.0	0.0	2.0061376041345906E-5	1.6230448985365147	1.0431915541499873E-4
42-43	0.0013882472220611368	0.0	0.0	2.0061376041345906E-5	1.6959552949086474	1.1501855597038319E-4
44-45	0.0015634499061555579	0.0	0.0	2.0061376041345906E-5	1.7683180157148515	1.2237439385221003E-4
46-47	0.0017386525902499787	0.0	0.0	2.1398801110768967E-5	1.840837215254178	1.3173636933817146E-4
48-49	0.0019332479378510337	0.0	0.0	2.1398801110768967E-5	1.913427967034719	1.3976091975470984E-4
50-51	0.0021084506219454548	0.0	0.0	2.407365124961509E-5	1.9858167676297769	1.4778547017124818E-4
52-53	0.0022910091439217028	0.0	0.0	2.407365124961509E-5	2.058562660718371	1.5982229579605573E-4
54-55	0.002472898953363239	0.0	0.0	2.407365124961509E-5	2.1313419894337002	1.638345710043249E-4
56-57	0.0026808685516585247	0.0	0.0	2.607978885374968E-5	2.2036385076989675	1.725278339555748E-4
58-59	0.002877470036863715	0.0	0.0	2.942335152730733E-5	2.27589022222441	1.8456465958038235E-4
60-61	0.003104832298665635	0.0	0.0	2.942335152730733E-5	2.347976096041243	1.9994504787874755E-4
62-63	0.003338881685814671	0.0	0.0	3.142948913144192E-5	2.4201114545856455	2.1331929857297814E-4
64-65	0.0035936611615397635	0.0	0.0	3.343562673557651E-5	2.4918047941446035	2.2869968687134335E-4
66-67	0.003857802612750818	0.0	0.0	3.611047687442263E-5	2.563668655399913	2.4876106291268923E-4
68-69	0.0041299686143784105	0.0	0.0	3.811661447855723E-5	2.635341264870294	2.641414512110545E-4
70-71	0.004423533417116772	0.0	0.0	3.8785327013268754E-5	2.7069389785367886	2.8888381499538106E-4
72-73	0.004757220971937827	0.0	0.0	3.8785327013268754E-5	2.7791632758483074	3.1696974145326533E-4
74-75	0.005060816462696861	0.0	0.0	4.0791464617403344E-5	2.851387573159826	3.3703111749461127E-4
76-77	0.005384473329497241	0.0	0.0	4.2797602221537934E-5	2.9231477839722553	3.731415943690339E-4
78-79	0.0057502590859844485	0.0	0.0	4.3466314756249466E-5	2.9946478656086812	4.1058949631287957E-4
80-81	0.006156167594554347	0.0	0.0	4.5472452360384056E-5	3.0668393960059994	4.3666928516662923E-4
82-83	0.006516603650763862	0.0	0.0	4.814730249923018E-5	3.1382512075878433	4.65423924159225E-4
84-85	0.006962634911416453	0.0	0.0	4.9484727568653236E-5	3.209607516029307	5.035405386377823E-4
86-87	0.007401310334187217	0.0	0.0	5.015344010336477E-5	3.2803927439661265	5.376448779080703E-4
88-89	0.007883452071714231	0.0	0.0	5.282829024221089E-5	3.351187333878432	5.744240673172045E-4
90-91	0.008395685873303263	0.0	0.0	5.483442784634548E-5	3.4220534760319516	6.252462199552809E-4
92-93	0.008929987188537776	0.0	0.0	5.483442784634548E-5	3.4929777961759916	6.780745101974918E-4
94-95	0.009556570833562478	0.0	0.0	5.483442784634548E-5	3.563886067219198	7.262218126967219E-4
96-97	0.010244007319245931	0.0	0.0	5.75092779851916E-5	3.634679988418969	7.723629775918175E-4
98-99	0.010950167755901307	0.0	0.0	6.152155319346079E-5	3.705580234911759	8.271974054381629E-4
100-101	0.011738579834326203	0.0	0.0	6.285897826288384E-5	3.77662826687472	8.673201575208546E-4
102-103	0.0126025564291735	0.0	0.0	6.352769079759537E-5	3.846895242597138	9.208171602977772E-4
104-105	0.013492612812874547	0.0	0.0	6.41964033323069E-5	3.9182542258887403	9.669583251928727E-4
106-107	0.014541822779836937	0.0	0.0	6.41964033323069E-5	3.989880694194227	0.0010217927530392183
108-109	0.01572343782867221	0.0	0.0	6.553382840172997E-5	4.060175087130569	0.0010900014315797942
110-111	0.017069556161046523	0.0	0.0	6.553382840172997E-5	4.130460118091424	0.001150185559703832
112-113	0.018509294248280447	0.0	0.0	6.620254093644149E-5	4.200636817621656	0.0012264187886609464
114-115	0.020068063166693025	0.0	0.0	6.687125347115302E-5	4.270339399964778	0.001298639742409792
116-117	0.02175388746670079	0.0	0.0	6.820867854057608E-5	4.3397758347190845	0.0013668484209503678
118-119	0.02365637462795509	0.0	0.0	6.887739107528762E-5	4.409685049235431	0.0014223515613314248
120-121	0.025704641121776513	0.0	0.0	7.08835286794222E-5	4.479806914337818	0.0014979160777538279
122-123	0.02803844786791975	0.0	0.0	7.355837881826833E-5	4.5496044538608675	0.0015781615819192114
124-125	0.030584236487566547	0.0	0.0	7.489580388769139E-5	4.61980388961728	0.0016543948108763257
126-127	0.033420246347278144	0.0	0.0	7.556451642240291E-5	4.689796024487932	0.001759382678826036
128-129	0.03662605423868522	0.0	0.0	7.623322895711444E-5	4.760147257989724	0.0018543398587550735
130-131	0.04025716330216883	0.0	0.0	7.623322895711444E-5	4.829877926259304	0.0019459534760105529
132-133	0.04424068387144542	0.0	0.0	7.757065402653751E-5	4.8995771650397515	0.002055622331703244
134-135	0.04865819887574979	0.0	0.0	8.024550416538362E-5	4.968910618063712	0.00215860406204882
136-137	0.05380126698021617	0.0	0.0	8.425777937365282E-5	5.0384834701751	0.002250217679304299
138-139	0.05946191858654927	0.0	0.0	8.62639169777874E-5	5.108139911353327	0.002345843571768048
>>END_MODULE
