SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR10065367_2.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.2
Python version: could not detect
Number of cores used for trimming: 2
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Illumina adapter for trimming (count: 444). Second best hit was smallRNA (count: 0)
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 4.2 with Python 3.10.8
Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065367_2.fastq.gz
Processing single-end reads on 2 cores ...
Finished in 1555.059 s (20.798 µs/read; 2.88 M reads/minute).

=== Summary ===

Total reads processed:              74,770,544
Reads with adapters:                35,848,044 (47.9%)
Reads written (passing filters):    74,770,544 (100.0%)

Total basepairs processed: 11,215,581,600 bp
Quality-trimmed:              84,913,645 bp (0.8%)
Total written (filtered):  11,092,777,790 bp (98.9%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 35848044 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 50.9%
  C: 16.7%
  G: 0.3%
  T: 32.1%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	35690764	18692636.0	0	35690764
2	38758	4673159.0	0	38758
3	31052	1168289.8	0	31052
4	11613	292072.4	0	11613
5	5232	73018.1	0	5232
6	3301	18254.5	0	3301
7	3498	4563.6	0	3498
8	2922	1140.9	0	2922
9	2640	285.2	0	2633 7
10	3287	71.3	1	2935 352
11	2560	17.8	1	2152 408
12	2703	4.5	1	2413 290
13	2266	1.1	1	2004 262
14	2922	1.1	1	2635 287
15	1609	1.1	1	1451 158
16	1933	1.1	1	1731 202
17	2235	1.1	1	2032 203
18	1155	1.1	1	1039 116
19	1974	1.1	1	1797 177
20	1295	1.1	1	1160 135
21	1205	1.1	1	1058 147
22	1295	1.1	1	1166 129
23	1328	1.1	1	1190 138
24	2212	1.1	1	1963 249
25	918	1.1	1	783 135
26	929	1.1	1	795 134
27	873	1.1	1	741 132
28	1222	1.1	1	1065 157
29	752	1.1	1	651 101
30	1319	1.1	1	1157 162
31	514	1.1	1	420 94
32	875	1.1	1	766 109
33	809	1.1	1	688 121
34	690	1.1	1	579 111
35	740	1.1	1	632 108
36	630	1.1	1	552 78
37	873	1.1	1	733 140
38	575	1.1	1	495 80
39	497	1.1	1	402 95
40	609	1.1	1	528 81
41	487	1.1	1	416 71
42	721	1.1	1	582 139
43	379	1.1	1	308 71
44	623	1.1	1	512 111
45	449	1.1	1	363 86
46	348	1.1	1	274 74
47	507	1.1	1	424 83
48	358	1.1	1	265 93
49	370	1.1	1	292 78
50	371	1.1	1	312 59
51	322	1.1	1	250 72
52	345	1.1	1	269 76
53	335	1.1	1	229 106
54	507	1.1	1	390 117
55	205	1.1	1	169 36
56	309	1.1	1	229 80
57	631	1.1	1	497 134
58	215	1.1	1	156 59
59	178	1.1	1	132 46
60	269	1.1	1	208 61
61	216	1.1	1	153 63
62	232	1.1	1	170 62
63	775	1.1	1	596 179
64	186	1.1	1	132 54
65	127	1.1	1	80 47
66	117	1.1	1	87 30
67	210	1.1	1	155 55
68	161	1.1	1	112 49
69	187	1.1	1	141 46
70	206	1.1	1	141 65
71	204	1.1	1	137 67
72	223	1.1	1	165 58
73	250	1.1	1	182 68
74	271	1.1	1	188 83
75	381	1.1	1	268 113
76	1097	1.1	1	934 163
77	360	1.1	1	287 73
78	163	1.1	1	110 53
79	136	1.1	1	82 54
80	109	1.1	1	64 45
81	99	1.1	1	56 43
82	80	1.1	1	46 34
83	90	1.1	1	52 38
84	76	1.1	1	41 35
85	59	1.1	1	24 35
86	77	1.1	1	49 28
87	59	1.1	1	27 32
88	66	1.1	1	35 31
89	60	1.1	1	33 27
90	60	1.1	1	32 28
91	45	1.1	1	23 22
92	42	1.1	1	24 18
93	50	1.1	1	25 25
94	48	1.1	1	27 21
95	49	1.1	1	31 18
96	52	1.1	1	22 30
97	35	1.1	1	23 12
98	33	1.1	1	16 17
99	42	1.1	1	21 21
100	34	1.1	1	16 18
101	34	1.1	1	18 16
102	36	1.1	1	15 21
103	33	1.1	1	12 21
104	28	1.1	1	15 13
105	40	1.1	1	25 15
106	23	1.1	1	14 9
107	23	1.1	1	12 11
108	34	1.1	1	23 11
109	22	1.1	1	12 10
110	22	1.1	1	7 15
111	26	1.1	1	13 13
112	27	1.1	1	15 12
113	24	1.1	1	11 13
114	23	1.1	1	14 9
115	33	1.1	1	16 17
116	14	1.1	1	9 5
117	32	1.1	1	25 7
118	17	1.1	1	12 5
119	26	1.1	1	16 10
120	22	1.1	1	11 11
121	26	1.1	1	12 14
122	12	1.1	1	7 5
123	22	1.1	1	10 12
124	20	1.1	1	13 7
125	18	1.1	1	10 8
126	16	1.1	1	9 7
127	17	1.1	1	9 8
128	6	1.1	1	4 2
129	11	1.1	1	5 6
130	8	1.1	1	5 3
131	10	1.1	1	6 4
132	6	1.1	1	1 5
133	4	1.1	1	2 2
134	5	1.1	1	4 1
135	4	1.1	1	2 2
136	4	1.1	1	3 1
138	2	1.1	1	0 2
140	1	1.1	1	0 1
142	1	1.1	1	1
143	1	1.1	1	0 1
145	1	1.1	1	1
147	2	1.1	1	1 1
148	1	1.1	1	0 1
149	4	1.1	1	0 4
150	23	1.1	1	2 21

RUN STATISTICS FOR INPUT FILE: SRR10065367_2.fastq.gz
=============================================
74770544 sequences processed in total

Total number of sequences analysed for the sequence pair length validation: 74770544

Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 40288 (0.05%)