##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065367_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74770544
Total Bases	11.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14562732083372	32.0	32.0	32.0	32.0	32.0
2	31.422772489123524	32.0	32.0	32.0	32.0	32.0
3	35.0230099703434	37.0	32.0	37.0	32.0	37.0
4	35.79537408474653	37.0	37.0	37.0	32.0	37.0
5	36.125528898117956	37.0	37.0	37.0	37.0	37.0
6	39.61654775442051	41.0	41.0	41.0	37.0	41.0
7	39.79193032218677	41.0	41.0	41.0	37.0	41.0
8	39.89999473589493	41.0	41.0	41.0	37.0	41.0
9	39.93273828527983	41.0	41.0	41.0	37.0	41.0
10-14	39.960479763260786	41.0	41.0	41.0	37.0	41.0
15-19	39.94360529729462	41.0	41.0	41.0	37.0	41.0
20-24	39.90514247161289	41.0	41.0	41.0	37.0	41.0
25-29	39.77181079222855	41.0	41.0	41.0	37.0	41.0
30-34	39.675710517767534	41.0	41.0	41.0	37.0	41.0
35-39	39.62483544321946	41.0	41.0	41.0	37.0	41.0
40-44	39.548464502277795	41.0	41.0	41.0	37.0	41.0
45-49	39.49917700745898	41.0	41.0	41.0	37.0	41.0
50-54	39.420635414930246	41.0	41.0	41.0	37.0	41.0
55-59	39.34220899609878	41.0	41.0	41.0	37.0	41.0
60-64	39.24592196627592	41.0	41.0	41.0	37.0	41.0
65-69	39.13131020151465	41.0	41.0	41.0	37.0	41.0
70-74	39.0486390656727	41.0	41.0	41.0	37.0	41.0
75-79	38.87850274033047	41.0	40.2	41.0	36.0	41.0
80-84	39.119627911226644	41.0	41.0	41.0	37.0	41.0
85-89	39.031213037583356	41.0	41.0	41.0	37.0	41.0
90-94	38.92567753686532	41.0	41.0	41.0	37.0	41.0
95-99	38.798604172252645	41.0	41.0	41.0	33.0	41.0
100-104	38.6661795933971	41.0	41.0	41.0	32.0	41.0
105-109	38.53804012178914	41.0	41.0	41.0	32.0	41.0
110-114	38.39036960865231	41.0	39.4	41.0	32.0	41.0
115-119	38.23622262531619	41.0	37.0	41.0	32.0	41.0
120-124	37.99690563973963	41.0	37.0	41.0	32.0	41.0
125-129	37.81691859296891	41.0	37.0	41.0	32.0	41.0
130-134	37.58471755401432	41.0	37.0	41.0	27.0	41.0
135-139	37.30752990107976	41.0	37.0	41.0	27.0	41.0
140-144	37.05850778349292	41.0	37.0	41.0	27.0	41.0
145-149	36.79665599062646	41.0	37.0	41.0	27.0	41.0
150	36.63030540475939	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	12.0
13	15.0
14	24.0
15	86.0
16	374.0
17	1222.0
18	2804.0
19	4780.0
20	8489.0
21	14278.0
22	23941.0
23	40311.0
24	67192.0
25	109079.0
26	168635.0
27	253032.0
28	365776.0
29	509379.0
30	689539.0
31	915566.0
32	1186068.0
33	1519317.0
34	1945283.0
35	2506815.0
36	3320766.0
37	4662355.0
38	7385020.0
39	1.5156101E7
40	3.3914285E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.837174968631933	40.83118220599488	43.979591034904296	0.3520517904688821
2	17.11665826687696	39.962580311077495	42.67418168654858	0.2465797354969683
3	15.29475182772327	37.324919434529406	47.084042536615826	0.29628620113151094
4	16.035958687675496	36.32269378377037	47.33374413639268	0.30760339216145494
5	16.43007706350244	36.201274662385764	47.066168195860655	0.3024800782511359
6	16.52573203389536	35.705709468572486	47.44901794731046	0.31954055022169914
7	16.961947416539306	35.920594139774124	46.81578428793913	0.3016741557474329
8	16.230605863250734	35.421072814029316	48.03862847793414	0.30969284478581244
9	16.539470396074492	35.320110944965464	47.831622685472944	0.30879597348710364
10-14	16.444055445354124	35.84490520566582	47.41632013761689	0.2947192113631645
15-19	16.43198947479771	35.481544393546265	47.78921424889238	0.29725188276363984
20-24	16.62671752161095	35.43621291076347	47.63434459969011	0.30272496793546844
25-29	16.764584662642097	35.24454297074008	47.678642647511744	0.31222971910607966
30-34	16.87372600273278	35.210344594403	47.60152584808897	0.31440355477524784
35-39	16.906548964255734	35.11572077328325	47.66061349254769	0.3171167699133318
40-44	16.90639420658509	35.14896090009654	47.62481481027547	0.3198300830428994
45-49	16.905907162002805	35.12494883618812	47.649770540568575	0.31937346124050026
50-54	16.905272308363646	35.13437167006559	47.63909478422764	0.3212612373431247
55-59	16.908520811271995	35.140746155683495	47.6264479701402	0.3242850629043128
60-64	16.88587026564256	35.141441070045666	47.64623174066016	0.32645692365161416
65-69	16.896997543604286	35.14709030843933	47.6270123923976	0.32889975555877493
70-74	16.888024993581933	35.14793182658916	47.63105584543586	0.33298733439305006
75-79	16.889112829392108	35.14492820397784	47.63956848584628	0.326390480783772
80-84	16.884078546390974	35.13405334218474	47.654941537074876	0.3269265743494125
85-89	16.900896037921235	35.13574259310738	47.620992536884756	0.3423688320866276
90-94	16.878177114040657	35.13026952472724	47.65193791309379	0.33961544813831124
95-99	16.886788417266633	35.147952423659554	47.618766474498045	0.34649268457576704
100-104	16.86162362869285	35.140825088314706	47.64966453000706	0.347886752985391
105-109	16.86881777507515	35.146359070884806	47.6278344202086	0.3569887338314497
110-114	16.862381508126216	35.1495622401988	47.633303611280866	0.35475264039411414
115-119	16.844483576302213	35.14307146149921	47.6492934330366	0.3631515291619751
120-124	16.83777327161106	35.14166479589332	47.644354666463926	0.3762072660316948
125-129	16.84131216405093	35.14515413620117	47.629488311452405	0.38404538829549345
130-134	16.827760462818894	35.144212874813356	47.63082711808559	0.3971995442821563
135-139	16.832442724706688	35.124128584304565	47.63084293936169	0.4125857516270619
140-144	16.819295354610762	35.1243021225709	47.634654652194406	0.4217478706239309
145-149	16.811722303393502	35.10868085300797	47.64808036721433	0.4315164763842051
150	16.802325136597844	35.12178258974498	47.64211330394429	0.43377896971288676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4823.0
1	6591.0
2	12552.5
3	26928.0
4	59471.0
5	130870.0
6	270515.0
7	518880.5
8	919684.0
9	1495230.0
10	2238655.0
11	3101194.0
12	3993689.0
13	4808060.5
14	5448914.5
15	5848943.0
16	5978358.5
17	5849066.0
18	5493295.5
19	4967116.5
20	4349066.5
21	3702926.5
22	3079694.0
23	2517451.0
24	2034076.5
25	1631966.5
26	1301453.0
27	1031240.5
28	814032.5
29	644869.5
30	514508.5
31	409983.5
32	321741.5
33	250304.5
34	195117.5
35	153283.5
36	121095.0
37	95946.0
38	76631.0
39	61728.5
40	49698.0
41	39772.0
42	31702.5
43	25412.0
44	20568.5
45	16591.5
46	13452.5
47	11005.0
48	9264.0
49	8999.5
50	9371.0
51	8877.5
52	7516.0
53	6017.0
54	4938.0
55	4088.0
56	3455.0
57	3036.5
58	2808.0
59	2608.0
60	2189.0
61	1769.0
62	1485.0
63	1147.0
64	861.5
65	670.5
66	527.0
67	431.0
68	368.0
69	305.0
70	267.0
71	228.0
72	179.5
73	145.5
74	123.5
75	99.0
76	101.5
77	106.5
78	127.0
79	132.0
80	106.5
81	81.5
82	68.5
83	66.5
84	47.5
85	43.5
86	41.0
87	31.5
88	30.0
89	22.0
90	11.5
91	9.5
92	9.0
93	9.0
94	9.5
95	7.5
96	6.0
97	16.5
98	27.0
99	79.5
100	950.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7730998988050696
2	0.0010900014315797942
3	8.144918672786438E-4
4	0.00857824439527951
5	0.0
6	8.693262951249893E-5
7	0.0010672652053996022
8	1.0699400555384485E-5
9	1.859020846498054E-4
10-14	2.5304082313484306E-4
15-19	2.9236112017588106E-4
20-24	3.116200411755731E-4
25-29	0.001481866976920751
30-34	3.608372837303417E-4
35-39	9.843448510953726E-4
40-44	6.243100224066847E-4
45-49	0.0012036825624807544
50-54	6.556057690311842E-4
55-59	2.9102369510645797E-4
60-64	9.904970064147187E-4
65-69	0.0011127376577599864
70-74	4.98592065880917E-4
75-79	3.8437596495218755E-4
80-84	0.0017092292387226712
85-89	0.0011087253825517172
90-94	5.97829006032108E-4
95-99	0.0018007091134712087
100-104	0.0030054616160075016
105-109	2.3164202202407408E-4
110-114	3.314139322030344E-4
115-119	5.796400250879545E-4
120-124	2.912911801203426E-4
125-129	0.001471702546393136
130-134	0.0010303522734835258
135-139	0.0016891678626813253
140-144	0.001197797892175293
145-149	0.0018028489935822856
150	0.005825823602406852
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.4770544E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.68072132586326
#Duplication Level	Percentage of total
1	74.61451304372767
2	15.968485808680963
3	3.9316069469525754
4	1.3131868754634364
5	0.6640974214376494
6	0.466118890406657
7	0.3711683011250528
8	0.29414542489952306
9	0.2662352720844922
>10	1.756189164372084
>50	0.12691647001735923
>100	0.1310285333640265
>500	0.05675377952160021
>1k	0.028717112419293065
>5k	0.010836955527662036
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.808592645788427E-5	0.0	0.0	0.0	0.0033943848261957276	0.00143773194962979
2	2.942335152730733E-5	0.0	0.0	2.674850138846121E-6	0.011176861305168517	0.0051851969941532055
3	3.0760776596730395E-5	0.0	0.0	2.674850138846121E-6	0.01790812167957478	0.007838648331888558
4	3.209820166615345E-5	0.0	0.0	4.0122752082691815E-6	0.024604609002176044	0.010905364016075635
5	3.343562673557651E-5	0.0	0.0	4.0122752082691815E-6	0.03143216398158077	0.014334521894076363
6	3.343562673557651E-5	0.0	0.0	4.0122752082691815E-6	0.038164761781056454	0.018163569867834586
7	4.012275208269181E-5	0.0	0.0	4.0122752082691815E-6	0.04466063534324426	0.021949820239371268
8	4.814730249923018E-5	0.0	0.0	5.349700277692242E-6	0.05087698706592265	0.02572537121035257
9	5.215957770749936E-5	0.0	0.0	8.024550416538363E-6	0.057348786976860834	0.02989011287653598
10-11	5.215957770749936E-5	0.0	0.0	9.361975485961424E-6	0.06633962700605736	0.036093759061054845
12-13	5.349700277692242E-5	0.0	0.0	1.0699400555384484E-5	0.07730517515025703	0.04460446349032849
14-15	5.951541558932619E-5	0.0	0.0	1.0699400555384484E-5	0.08767690656363286	0.05376983749108473
16-17	7.222095374884526E-5	0.0	0.0	1.1368113090096014E-5	0.09793963783385072	0.0630709601363874
18-19	8.358906683894127E-5	0.0	0.0	1.2036825624807545E-5	0.10776436239383252	0.07236004595606527
20-21	1.1167499329682554E-4	0.0	0.0	1.2705538159519075E-5	0.11697654627201856	0.08151940154400909
22-23	1.283928066646138E-4	0.0	0.0	1.3374250694230606E-5	0.1261425889853095	0.09052227839883042
24-25	1.584848707266327E-4	0.0	0.0	1.4042963228942136E-5	0.13499500551982074	0.09942351094837559
26-27	1.7988367183740164E-4	0.0	0.0	1.4711675763653665E-5	0.14377921337579141	0.10834146131128858
28-29	1.9793891027461294E-4	0.0	0.0	1.4711675763653665E-5	0.1525647586568315	0.11706949731434346
30-31	2.3471809968374712E-4	0.0	0.0	1.8055238437211316E-5	0.1613409419623856	0.12581491984330087
32-33	3.029267782243232E-4	0.0	0.0	1.8723950971922848E-5	0.1701438737693282	0.13467134865302038
34-35	3.4639309298057264E-4	0.0	0.0	1.8723950971922848E-5	0.17895683626429146	0.14333986924048592
36-37	3.8317228238970687E-4	0.0	0.0	1.8723950971922848E-5	0.18796974380713347	0.1520358070418747
38-39	4.346631475624947E-4	0.0	0.0	1.8723950971922848E-5	0.19717791541011123	0.16059465877364754
40-41	4.841478751311479E-4	0.0	0.0	1.8723950971922848E-5	0.20636134464930467	0.1692491363978842
42-43	5.195896394708591E-4	1.3374250694230604E-6	0.0	1.8723950971922848E-5	0.2154912768857212	0.17783741148118434
44-45	5.663995169006661E-4	1.3374250694230604E-6	0.0	1.8723950971922848E-5	0.2246419392107138	0.18656678490930867
46-47	6.259149324899923E-4	1.3374250694230604E-6	0.0	1.8723950971922848E-5	0.23390628266660732	0.19528077794913462
48-49	6.901113358222992E-4	1.3374250694230604E-6	0.0	2.2067513645480496E-5	0.24322144827513895	0.2041532558596872
50-51	7.436083385992217E-4	1.3374250694230604E-6	0.0	2.2736226180192028E-5	0.2526161906753012	0.2128231138722222
52-53	7.95767916306721E-4	1.3374250694230604E-6	0.0	2.2736226180192028E-5	0.2620109330754635	0.22160732172819286
54-55	8.62639169777874E-4	1.3374250694230604E-6	0.0	2.4742363784326618E-5	0.271523368881735	0.23053864634180005
56-57	9.275042856448925E-4	1.3374250694230604E-6	0.0	2.541107631903815E-5	0.2808866817927659	0.2392452835437442
58-59	9.86350988699507E-4	1.3374250694230604E-6	0.0	2.541107631903815E-5	0.29055292148202105	0.24802748526211071
60-61	0.0010558970923095063	2.674850138846121E-6	0.0	2.541107631903815E-5	0.3001736887189158	0.25678226976655405
62-63	0.0011294554711277745	2.674850138846121E-6	0.0	2.541107631903815E-5	0.3097509896410544	0.26576441653279936
64-65	0.0012190629507791196	2.674850138846121E-6	0.0	2.8085926457884272E-5	0.31944130298155915	0.27485422601713316
66-67	0.0013146888432428684	3.343562673557651E-6	0.0	2.942335152730733E-5	0.32921788023904175	0.2839513913393488
68-69	0.0014250264114702709	4.0122752082691815E-6	0.0	2.942335152730733E-5	0.3392565928101312	0.2932371335963531
70-71	0.0015601063434820001	4.0122752082691815E-6	0.0	2.942335152730733E-5	0.3491863052380627	0.3023496793068672
72-73	0.0017005359757714215	4.0122752082691815E-6	0.0	3.0760776596730395E-5	0.3592771773868597	0.3116835688663707
74-75	0.0018275913573666122	4.0122752082691815E-6	0.0	3.0760776596730395E-5	0.36954927063256354	0.3211164278810116
76-77	0.001966014852051899	4.0122752082691815E-6	0.0	3.0760776596730395E-5	0.3796555231696589	0.3305285568070763
78-79	0.002137873973472762	4.0122752082691815E-6	0.0	3.0760776596730395E-5	0.38967083080203346	0.34007041596487514
80-81	0.0023271196207961253	4.0122752082691815E-6	0.0	3.0760776596730395E-5	0.3997857766020801	0.3494785326157317
82-83	0.0025330830814872763	4.0122752082691815E-6	0.0	3.0760776596730395E-5	0.40994485742941766	0.35886993145322044
84-85	0.0027617827683586197	4.0122752082691815E-6	0.0	3.343562673557651E-5	0.42019755801161485	0.3684318519870606
86-87	0.003012549968875444	4.0122752082691815E-6	0.0	3.4773051804999574E-5	0.4304495898812773	0.3778526741760766
88-89	0.0033061147716138056	4.0122752082691815E-6	0.0	3.4773051804999574E-5	0.4406340817849339	0.3872420668759612
90-91	0.0036163973877199555	4.0122752082691815E-6	0.0	3.4773051804999574E-5	0.4510726844517809	0.3966187540376863
92-93	0.003952091080145144	4.0122752082691815E-6	0.0	3.611047687442263E-5	0.4615032625682114	0.40602486455093867
94-95	0.004362680576458023	4.0122752082691815E-6	0.0	3.6779189409134164E-5	0.4719044173331145	0.4152591159427702
96-97	0.00485953398974869	4.0122752082691815E-6	0.0	3.7447901943845696E-5	0.48254497118544437	0.4246097233156415
98-99	0.005386479467101376	4.0122752082691815E-6	0.0	3.811661447855723E-5	0.4932824883553074	0.43372427516375966
100-101	0.005979627485390503	4.0122752082691815E-6	0.0	3.945403954798028E-5	0.5041590977323904	0.44313506666475505
102-103	0.006635634481942515	4.0122752082691815E-6	0.0	4.012275208269181E-5	0.5151119403384306	0.4530507361294576
104-105	0.007308359291862314	4.0122752082691815E-6	0.0	4.012275208269181E-5	0.5262105622770379	0.4626567916905887
106-107	0.00813288184716163	4.0122752082691815E-6	0.0	4.012275208269181E-5	0.5374282150468238	0.47236047928178776
108-109	0.009069748108292485	4.680987742980712E-6	0.0	4.1460177152114876E-5	0.5485810027007427	0.48226143707072666
110-111	0.010148381426782184	5.349700277692242E-6	0.0	4.1460177152114876E-5	0.5596943363151137	0.4922132170123037
112-113	0.011352732701797649	5.349700277692242E-6	0.0	4.2797602221537934E-5	0.5709561241121905	0.501953015080377
114-115	0.012664746694901672	5.349700277692242E-6	0.0	4.2797602221537934E-5	0.5820707951516308	0.5116995002737976
116-117	0.014107159632274442	5.349700277692242E-6	0.0	4.2797602221537934E-5	0.5933533130372838	0.5213115742477412
118-119	0.01574149306710942	5.349700277692242E-6	0.0	4.2797602221537934E-5	0.6044372233001274	0.5309330101971708
120-121	0.017554372748712382	5.349700277692242E-6	0.0	4.4135027290961E-5	0.6157692259133489	0.5405811946479886
122-123	0.01963540615673466	5.349700277692242E-6	0.0	4.5472452360384056E-5	0.627186155018479	0.5504828211494623
124-125	0.02196185706499608	5.349700277692242E-6	0.0	4.5472452360384056E-5	0.6385014398183327	0.5603369690609714
126-127	0.024564486250093352	5.349700277692242E-6	0.0	4.680987742980712E-5	0.6499691910761007	0.5700440002148439
128-129	0.02751818951591418	5.349700277692242E-6	0.0	4.680987742980712E-5	0.6615532983149086	0.5798038596589588
130-131	0.030907893354366927	5.349700277692242E-6	0.0	4.680987742980712E-5	0.6732283504584372	0.5894988539872065
132-133	0.03464599642340438	5.349700277692242E-6	0.0	4.680987742980712E-5	0.6849274762532154	0.5993115417215635
134-135	0.03884484242885808	5.349700277692242E-6	0.0	4.680987742980712E-5	0.6964734668775447	0.609007204762346
136-137	0.04376522925926552	5.349700277692242E-6	0.0	4.680987742980712E-5	0.7082728995525297	0.6185136221558051
138-139	0.049213898992095065	5.349700277692242E-6	0.0	4.814730249923018E-5	0.7201004181539725	0.6282253342974207
>>END_MODULE
