SUMMARISING RUN PARAMETERS ========================== Input filename: SRR10065367_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 444). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065367_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1423.006 s (19.032 µs/read; 3.15 M reads/minute). === Summary === Total reads processed: 74,770,544 Reads with adapters: 31,009,620 (41.5%) Reads written (passing filters): 74,770,544 (100.0%) Total basepairs processed: 11,215,581,600 bp Quality-trimmed: 20,945,866 bp (0.2%) Total written (filtered): 11,153,527,853 bp (99.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 31009620 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 41.5% C: 0.1% G: 12.9% T: 45.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 24795978 18692636.0 0 24795978 2 4071014 4673159.0 0 4071014 3 1498450 1168289.8 0 1498450 4 579098 292072.4 0 579098 5 4582 73018.1 0 4582 6 4371 18254.5 0 4371 7 3210 4563.6 0 3210 8 2815 1140.9 0 2815 9 3514 285.2 0 2620 894 10 4068 71.3 1 2559 1509 11 2632 17.8 1 2261 371 12 2851 4.5 1 2591 260 13 1951 1.1 1 1867 84 14 2104 1.1 1 1988 116 15 1730 1.1 1 1669 61 16 1861 1.1 1 1758 103 17 1522 1.1 1 1448 74 18 1662 1.1 1 1566 96 19 1348 1.1 1 1274 74 20 1288 1.1 1 1235 53 21 1273 1.1 1 1214 59 22 1160 1.1 1 1107 53 23 1139 1.1 1 1077 62 24 1319 1.1 1 1221 98 25 932 1.1 1 895 37 26 941 1.1 1 895 46 27 861 1.1 1 825 36 28 798 1.1 1 755 43 29 813 1.1 1 762 51 30 769 1.1 1 740 29 31 725 1.1 1 689 36 32 612 1.1 1 574 38 33 629 1.1 1 595 34 34 611 1.1 1 587 24 35 545 1.1 1 516 29 36 572 1.1 1 533 39 37 528 1.1 1 487 41 38 499 1.1 1 463 36 39 435 1.1 1 415 20 40 467 1.1 1 445 22 41 487 1.1 1 448 39 42 376 1.1 1 352 24 43 433 1.1 1 406 27 44 346 1.1 1 323 23 45 449 1.1 1 429 20 46 133 1.1 1 123 10 47 221 1.1 1 205 16 48 246 1.1 1 224 22 49 256 1.1 1 241 15 50 282 1.1 1 264 18 51 198 1.1 1 181 17 52 262 1.1 1 241 21 53 201 1.1 1 185 16 54 208 1.1 1 191 17 55 255 1.1 1 232 23 56 145 1.1 1 133 12 57 181 1.1 1 165 16 58 133 1.1 1 119 14 59 119 1.1 1 110 9 60 124 1.1 1 111 13 61 112 1.1 1 102 10 62 133 1.1 1 120 13 63 139 1.1 1 124 15 64 127 1.1 1 113 14 65 72 1.1 1 60 12 66 143 1.1 1 131 12 67 100 1.1 1 84 16 68 87 1.1 1 78 9 69 105 1.1 1 95 10 70 130 1.1 1 115 15 71 223 1.1 1 152 71 72 638 1.1 1 603 35 73 156 1.1 1 142 14 74 39 1.1 1 32 7 75 36 1.1 1 30 6 76 26 1.1 1 19 7 77 25 1.1 1 19 6 78 20 1.1 1 16 4 79 24 1.1 1 18 6 80 23 1.1 1 20 3 81 22 1.1 1 18 4 82 24 1.1 1 21 3 83 19 1.1 1 13 6 84 19 1.1 1 14 5 85 12 1.1 1 8 4 86 27 1.1 1 23 4 87 18 1.1 1 11 7 88 15 1.1 1 9 6 89 14 1.1 1 9 5 90 8 1.1 1 6 2 91 14 1.1 1 10 4 92 12 1.1 1 10 2 93 11 1.1 1 10 1 94 7 1.1 1 4 3 95 13 1.1 1 11 2 96 10 1.1 1 8 2 97 16 1.1 1 6 10 98 5 1.1 1 3 2 99 6 1.1 1 2 4 100 7 1.1 1 6 1 101 10 1.1 1 8 2 102 10 1.1 1 3 7 103 15 1.1 1 10 5 104 8 1.1 1 6 2 105 7 1.1 1 1 6 106 3 1.1 1 3 107 8 1.1 1 6 2 108 3 1.1 1 2 1 109 5 1.1 1 4 1 110 6 1.1 1 3 3 111 5 1.1 1 3 2 112 14 1.1 1 7 7 113 2 1.1 1 2 114 6 1.1 1 3 3 115 8 1.1 1 4 4 116 4 1.1 1 2 2 117 7 1.1 1 6 1 118 2 1.1 1 1 1 119 16 1.1 1 12 4 120 2 1.1 1 1 1 121 5 1.1 1 4 1 122 4 1.1 1 3 1 123 3 1.1 1 2 1 124 5 1.1 1 1 4 125 3 1.1 1 3 126 6 1.1 1 4 2 127 3 1.1 1 2 1 128 1 1.1 1 1 129 5 1.1 1 3 2 130 4 1.1 1 1 3 131 4 1.1 1 3 1 132 2 1.1 1 0 2 133 3 1.1 1 0 3 134 1 1.1 1 0 1 135 3 1.1 1 0 3 136 3 1.1 1 0 3 137 3 1.1 1 0 3 138 1 1.1 1 0 1 140 1 1.1 1 0 1 142 2 1.1 1 1 1 143 2 1.1 1 2 145 1 1.1 1 0 1 147 1 1.1 1 0 1 148 3 1.1 1 0 3 149 4 1.1 1 0 4 150 37 1.1 1 4 33 RUN STATISTICS FOR INPUT FILE: SRR10065367_1.fastq.gz ============================================= 74770544 sequences processed in total