##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065366_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81490382
Total Bases	12.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.788674938350393	32.0	32.0	32.0	32.0	32.0
2	31.139195776502802	32.0	32.0	32.0	32.0	32.0
3	34.54421902206815	37.0	32.0	37.0	32.0	37.0
4	35.152975218596964	37.0	37.0	37.0	32.0	37.0
5	35.66932562667334	37.0	37.0	37.0	32.0	37.0
6	39.111720791786205	41.0	41.0	41.0	37.0	41.0
7	39.33415776600483	41.0	41.0	41.0	37.0	41.0
8	39.36944761162121	41.0	41.0	41.0	37.0	41.0
9	39.32630184504473	41.0	41.0	41.0	37.0	41.0
10-14	39.43694010171654	41.0	41.0	41.0	37.0	41.0
15-19	39.37989783137843	41.0	41.0	41.0	37.0	41.0
20-24	39.303394221418664	41.0	41.0	41.0	37.0	41.0
25-29	39.23769726346355	41.0	41.0	41.0	37.0	41.0
30-34	39.16204824760793	41.0	41.0	41.0	37.0	41.0
35-39	39.07051093710667	41.0	41.0	41.0	37.0	41.0
40-44	38.99878292140046	41.0	41.0	41.0	37.0	41.0
45-49	38.90137955176109	41.0	41.0	41.0	37.0	41.0
50-54	38.76539306196896	41.0	41.0	41.0	35.0	41.0
55-59	38.64183382279396	41.0	41.0	41.0	32.0	41.0
60-64	38.52796974494487	41.0	41.0	41.0	32.0	41.0
65-69	38.42275245684822	41.0	41.0	41.0	32.0	41.0
70-74	38.29570601595658	41.0	41.0	41.0	32.0	41.0
75-79	38.065555638210164	41.0	39.4	41.0	31.0	41.0
80-84	38.25897238523192	41.0	41.0	41.0	32.0	41.0
85-89	38.12515576868937	41.0	41.0	41.0	32.0	41.0
90-94	37.937097028211255	41.0	37.0	41.0	29.0	41.0
95-99	37.727236613027536	41.0	37.0	41.0	27.0	41.0
100-104	37.5009306914281	41.0	37.0	41.0	27.0	41.0
105-109	37.241072461778366	41.0	37.0	41.0	27.0	41.0
110-114	37.02082732168319	41.0	37.0	41.0	27.0	41.0
115-119	36.74107747586703	41.0	37.0	41.0	26.0	41.0
120-124	36.37300321650228	41.0	37.0	41.0	22.0	41.0
125-129	35.986993397576654	41.0	36.0	41.0	22.0	41.0
130-134	35.60465171705785	41.0	32.0	41.0	22.0	41.0
135-139	35.13438578800624	41.0	32.0	41.0	22.0	41.0
140-144	34.54383805195563	37.8	32.0	41.0	18.0	41.0
145-149	33.944908983246634	37.0	32.0	41.0	12.0	41.0
150	33.552435537730084	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	64.0
12	766.0
13	2660.0
14	6297.0
15	15188.0
16	26532.0
17	38563.0
18	56160.0
19	87177.0
20	142868.0
21	234848.0
22	348321.0
23	461623.0
24	566705.0
25	673832.0
26	790539.0
27	920093.0
28	1063042.0
29	1211807.0
30	1364259.0
31	1514005.0
32	1671683.0
33	1873426.0
34	2185693.0
35	2719964.0
36	3613114.0
37	5161301.0
38	8151357.0
39	1.5575128E7
40	3.1013366E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.24800531069664333	54.49802094081492	28.245219568903124	17.008754179585313
2	0.2106806483597673	54.44218863416215	33.608295493464404	11.738835224013677
3	0.21316895720817475	48.69625895714215	35.590797527446746	15.499774558202922
4	0.255922554730244	47.8737038697601	35.38723076652726	16.4831428089824
5	0.2622376356620016	48.68489969531309	34.74423614812135	16.308626520903555
6	0.27883269479961886	48.365007841137974	34.9400028236564	16.416156640406008
7	0.2820689673283294	49.28779177962584	34.474074072619686	15.95606518042614
8	0.252396250701174	47.16739699858328	36.44903221822592	16.131174532489627
9	0.26639935104426954	47.20744543775895	36.62260432264755	15.903550888549237
10-14	0.2554349957587357	48.157753188703786	35.77574616284737	15.811065652690104
15-19	0.26261657708055636	48.016432446420595	35.80425308197952	15.916697894519327
20-24	0.28367944861650196	48.06685425121933	35.5697094860514	16.07975681411277
25-29	0.2875684234359994	48.08960800928986	35.41928412446208	16.203539442812065
30-34	0.27374521653685996	48.108311087843866	35.35240218178014	16.26554151383914
35-39	0.2820980164499955	48.09205136855092	35.325406409119694	16.300444205879387
40-44	0.27836335699680964	48.09546092630283	35.28922849265675	16.33694722404362
45-49	0.2810598910540549	48.072292017786395	35.28910733258888	16.35754075857068
50-54	0.2825964987510662	48.06121687811626	35.27559207325239	16.38059454988028
55-59	0.28864523962805844	48.04952038408444	35.26443110232989	16.39740327395762
60-64	0.29200498454418544	48.01342223444044	35.24779147445305	16.446781306562322
65-69	0.2978081519020526	47.98627515914905	35.247447018855055	16.468469670093835
70-74	0.3066614050942901	47.95609810753824	35.237705171674314	16.499535315693166
75-79	0.3115874219563433	47.932851056056684	35.23648753879181	16.519073983195163
80-84	0.3144176767520189	47.907096373058295	35.23624917602171	16.542236774167975
85-89	0.32062093243231654	47.87655773687815	35.23597331985779	16.56684801083175
90-94	0.3336397508266886	47.83476438388824	35.22528857220937	16.60630729307569
95-99	0.34235820815519635	47.79288940143333	35.215164275412555	16.649588114998927
100-104	0.3500825563442009	47.766328070111506	35.20195639800924	16.681632975535056
105-109	0.3593606269014969	47.70514862363384	35.194885907022396	16.74060484244226
110-114	0.3629821832276287	47.6804526112393	35.192902693350746	16.76366251218232
115-119	0.38721267932803244	47.637496567920806	35.16633890012249	16.808951852628674
120-124	0.3950900329746501	47.588845337142146	35.16235690278314	16.85370772710006
125-129	0.4151189389268904	47.56611735310791	35.132532958296885	16.886230749668314
130-134	0.4252404776206682	47.536328389671446	35.11320777254792	16.925223360159965
135-139	0.4511619723561603	47.487190683195415	35.09237972302665	16.96926762142177
140-144	0.47743572230944603	47.43702960679484	35.0726871708572	17.01284750003852
145-149	0.4912118395349051	47.37238687858589	35.04918344974869	17.087217832130513
150	0.5072178681915913	47.33753574226434	35.02486016052318	17.13038622902089
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5387.0
1	6919.0
2	12881.5
3	29234.0
4	70649.5
5	165296.5
6	354353.5
7	687527.0
8	1215146.5
9	1954341.0
10	2875694.0
11	3902093.0
12	4911386.5
13	5772539.5
14	6377405.0
15	6669746.0
16	6643596.0
17	6329057.0
18	5793265.0
19	5117722.5
20	4384509.5
21	3659192.5
22	2987966.0
23	2403646.0
24	1911648.0
25	1508151.0
26	1183400.5
27	923249.5
28	719350.5
29	562479.5
30	443079.5
31	349824.0
32	273252.0
33	212689.5
34	167654.5
35	133751.5
36	107601.0
37	87248.5
38	71595.5
39	59393.5
40	49577.0
41	41736.0
42	35368.0
43	30287.0
44	26113.5
45	22897.5
46	20170.5
47	17669.5
48	15712.5
49	14671.0
50	14020.0
51	13082.5
52	11890.0
53	10473.0
54	9474.5
55	8674.5
56	7832.5
57	7147.0
58	6628.0
59	6030.5
60	5571.0
61	5259.0
62	4908.5
63	4596.5
64	4246.5
65	3850.0
66	3553.0
67	3233.0
68	2864.0
69	2542.5
70	2255.0
71	2061.0
72	1930.5
73	1849.5
74	1703.5
75	1511.5
76	1402.5
77	1344.0
78	1276.5
79	1179.5
80	1145.0
81	1117.0
82	1021.0
83	950.5
84	863.0
85	798.5
86	760.0
87	728.0
88	728.0
89	729.5
90	743.0
91	709.0
92	651.5
93	649.5
94	663.5
95	675.0
96	704.5
97	766.5
98	940.0
99	1405.5
100	3346.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9581744260322647
2	0.0049306432260926205
3	0.0018407080236781807
4	0.0010872448726525787
5	2.1106785338176473E-4
6	3.6446018868827983E-4
7	3.4359883108659375E-5
8	3.2519175084981197E-4
9	8.516342456217717E-4
10-14	6.503835016996239E-4
15-19	7.718702312623838E-4
20-24	7.208212620723756E-4
25-29	2.5892626199739744E-4
30-34	1.8947021257060742E-4
35-39	2.3978289855114437E-4
40-44	0.002716885042948995
45-49	1.6541829439454584E-4
50-54	0.0021565735205413567
55-59	4.7122125406161427E-4
60-64	4.94782316764695E-4
65-69	0.0038735859650283638
70-74	0.0023811398994300946
75-79	0.0032217298969097973
80-84	4.550230234532463E-4
85-89	0.0023816307549030754
90-94	8.486891127838866E-4
95-99	7.927315888640699E-4
100-104	5.652200771374468E-4
105-109	0.00271393991011111
110-114	1.9192448993551165E-4
115-119	0.0031981688342067167
120-124	5.519669793669638E-4
125-129	0.0027814325376459767
130-134	6.03015948556972E-4
135-139	0.002071164668242689
140-144	0.0039052461430356283
145-149	0.0012502088896822203
150	0.012043139029585112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.1490382E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.2236584427171
#Duplication Level	Percentage of total
1	76.98823583036982
2	14.835292451800145
3	3.400705660105253
4	1.1423536944125199
5	0.6191564761740358
6	0.43210186992702176
7	0.36016390556399336
8	0.25499769237781045
9	0.22402893998509094
>10	1.4696549526401048
>50	0.10870721464501627
>100	0.12626237283924716
>500	0.013170361330507262
>1k	0.016343064030681604
>5k	0.008825513798719703
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.8224189696398773E-5	0.0	0.0	0.0	0.183278070778954	0.0
2	2.9451328378850892E-5	0.0	0.0	0.0	0.23403866237858598	2.454277364904241E-6
3	2.9451328378850892E-5	0.0	0.0	0.0	0.2695127874109119	6.135693412260603E-6
4	3.190560574375514E-5	0.0	0.0	0.0	0.31017034623791556	6.135693412260603E-6
5	3.190560574375514E-5	0.0	0.0	1.2271386824521206E-6	0.35215444198064993	7.362832094712723E-6
6	3.3132744426207256E-5	0.0	0.0	2.454277364904241E-6	0.3938243902206766	1.1044248142069085E-5
7	3.5587021791111494E-5	0.0	0.0	2.454277364904241E-6	0.43505870422843274	1.2271386824521206E-5
8	4.1722715203372096E-5	0.0	0.0	2.454277364904241E-6	0.4747934547662324	1.4725664189425446E-5
9	4.663126993318058E-5	0.0	0.0	3.6814160473563615E-6	0.5173800756020508	2.208849628413817E-5
10-11	5.031268598053694E-5	0.0	0.0	3.6814160473563615E-6	0.5807857177550106	2.5769912331494535E-5
12-13	5.8902656757701784E-5	0.0	0.0	3.6814160473563615E-6	0.663620646667235	3.8654868497241795E-5
14-15	6.871976621731875E-5	0.0	0.0	4.908554729808482E-6	0.7441356699002835	5.0926255321763006E-5
16-17	8.712684645410056E-5	0.0	0.0	4.908554729808482E-6	0.8244733715937176	5.583481005157148E-5
18-19	1.0553392669088236E-4	0.0	0.0	4.908554729808482E-6	0.9040833800484578	6.442478082873632E-5
20-21	1.3621239375218537E-4	0.0	0.0	5.522124071034543E-6	0.9833651289056419	7.240118226467511E-5
22-23	1.8897935709762657E-4	0.0	0.0	7.362832094712723E-6	1.0628616270322553	7.546902897080541E-5
24-25	2.4297345912551986E-4	0.0	0.0	7.362832094712723E-6	1.1421574879842875	8.283186106551813E-5
26-27	3.067846706130301E-4	0.0	0.0	7.362832094712723E-6	1.2217460215120846	8.589970777164844E-5
28-29	3.76731575512801E-4	0.0	0.0	7.976401435938784E-6	1.3006142246332826	8.896755447777873E-5
30-31	4.607905752607713E-4	0.0	0.0	8.589970777164844E-6	1.3796376607978105	9.694395591371752E-5
32-33	5.681652099753319E-4	0.0	0.0	8.589970777164844E-6	1.4588513034581185	1.0430678800843024E-4
34-35	6.902655088793178E-4	0.0	0.0	1.1044248142069085E-5	1.5373912469817603	1.1166962010314297E-4
36-37	8.13592946465756E-4	0.0	0.0	1.1044248142069085E-5	1.6165932318245853	1.2087316022153387E-4
38-39	9.289439826162553E-4	0.0	0.0	1.2271386824521206E-5	1.696384856804328	1.2394100692766417E-4
40-41	0.0010424543107430764	0.0	0.0	1.2271386824521206E-5	1.7759881159963147	1.2762242297502055E-4
42-43	0.0011510560841400891	0.0	0.0	1.2884956165747267E-5	1.8545440123228285	1.3498525506973325E-4
44-45	0.0012927906019633089	0.0	0.0	1.3498525506973327E-5	1.933319566473501	1.466430725530284E-4
46-47	0.0014283894263742683	0.0	6.135693412260603E-7	1.3498525506973327E-5	2.012014129471132	1.558466126714193E-4
48-49	0.0015787139149746532	0.0	1.2271386824521206E-6	1.3498525506973327E-5	2.0906657426148767	1.668908608134884E-4
50-51	0.0017130856007031604	0.0	1.2271386824521206E-6	1.4112094848199386E-5	2.1696198454438465	1.7241298488452294E-4
52-53	0.0018437758703843113	0.0	1.2271386824521206E-6	1.595280287187757E-5	2.2482051930987392	1.810029556616878E-4
54-55	0.0020149617165863823	0.0	1.2271386824521206E-6	1.7179941554329687E-5	2.3269500687823506	1.871386490739484E-4
56-57	0.0021873747014709045	0.0	1.2271386824521206E-6	1.7179941554329687E-5	2.404538366257751	1.9756932787479142E-4
58-59	0.002352424854260715	0.0	1.2271386824521206E-6	1.7179941554329687E-5	2.4816529882017244	2.1045428404053868E-4
60-61	0.0025389499339934377	0.0	1.2271386824521206E-6	1.7793510895555747E-5	2.558662076219007	2.190442548177035E-4
62-63	0.002715657904266543	0.0	1.2271386824521206E-6	1.8407080236781807E-5	2.63553617898122	2.2824779493609443E-4
64-65	0.0029077051080702997	0.0	1.2271386824521206E-6	1.8407080236781807E-5	2.7129631077198777	2.3376991900712896E-4
66-67	0.0031193865307932904	0.0	1.2271386824521206E-6	1.8407080236781807E-5	2.790483912567743	2.552448459500411E-4
68-69	0.003348247895070611	0.0	1.2271386824521206E-6	1.8407080236781807E-5	2.867411395862643	2.681298021157883E-4
70-71	0.0035918349235373567	0.0	1.2271386824521206E-6	1.8407080236781807E-5	2.943601368809389	2.804011889403095E-4
72-73	0.003847693338828624	0.0	1.2271386824521206E-6	1.8407080236781807E-5	3.020382822601077	3.000354078595435E-4
74-75	0.004142820191958359	0.0	1.2271386824521206E-6	2.0861357601686048E-5	3.0971790020569543	3.184424880963253E-4
76-77	0.004445309877182807	0.0	1.2271386824521206E-6	2.0861357601686048E-5	3.1738009278199235	3.442124004278198E-4
78-79	0.004766820211985262	0.0	1.2271386824521206E-6	2.208849628413817E-5	3.2497522222929325	3.6261948066460166E-4
80-81	0.005107964765706952	0.0	1.2271386824521206E-6	2.208849628413817E-5	3.325678360422952	3.8225369958383556E-4
82-83	0.0055135341002573775	0.0	1.2271386824521206E-6	2.208849628413817E-5	3.400890303839783	4.006607798206173E-4
84-85	0.005962053288693628	0.0	1.2271386824521206E-6	2.208849628413817E-5	3.476164217760079	4.264306921521119E-4
86-87	0.006444318790897311	0.0	1.2271386824521206E-6	2.208849628413817E-5	3.55093070983518	4.4913275777747617E-4
88-89	0.006959103468185976	0.0	1.2271386824521206E-6	2.331563496659029E-5	3.625346853816442	4.7612980879142277E-4
90-91	0.00749720378044123	0.0	1.2271386824521206E-6	2.331563496659029E-5	3.6994298296454176	4.99445443758013E-4
92-93	0.00817028934776622	0.0	1.2271386824521206E-6	2.3929204307816353E-5	3.7739146933928964	5.221475093833773E-4
94-95	0.008813923586712357	0.0	1.2271386824521206E-6	2.4542773649042412E-5	3.847503132332844	5.485309910560978E-4
96-97	0.009575976708515122	0.0	1.2271386824521206E-6	2.4542773649042412E-5	3.921046780710882	5.736873340463663E-4
98-99	0.010376071129473904	0.0	1.2271386824521206E-6	2.4542773649042412E-5	3.994794134110207	6.074336478137997E-4
100-101	0.0112988794186779	0.0	1.2271386824521206E-6	2.4542773649042412E-5	4.0689630096469545	6.399528228987809E-4
102-103	0.01230636027697109	0.0	1.2271386824521206E-6	2.4542773649042412E-5	4.142158764208517	6.706312899600839E-4
104-105	0.013398513704353479	0.0	1.2271386824521206E-6	2.515634299026847E-5	4.215211557113575	6.964012022915784E-4
106-107	0.01457718040884874	0.0	1.2271386824521206E-6	2.576991233149453E-5	4.287538497487962	7.233982533055251E-4
108-109	0.015893900215119865	0.0	1.2271386824521206E-6	2.6997051013946654E-5	4.359870959986418	7.602124137790887E-4
110-111	0.01727136338617237	0.0	1.2271386824521206E-6	2.7610620355172713E-5	4.432072118645854	7.915044501816177E-4
112-113	0.018800991753848938	0.0	1.2271386824521206E-6	2.8224189696398773E-5	4.504180014765423	8.326135960437639E-4
114-115	0.020533098004130106	0.0	1.2271386824521206E-6	2.8224189696398773E-5	4.576298955133135	8.669734791524231E-4
116-117	0.022467682137015874	0.0	1.2271386824521206E-6	2.9451328378850892E-5	4.647323901365439	9.05628347649665E-4
118-119	0.024569770700056358	0.0	1.2271386824521206E-6	3.006489772007695E-5	4.718528009845382	9.479646321942631E-4
120-121	0.026826478737085806	0.0	1.2271386824521206E-6	3.190560574375514E-5	4.789830289419922	0.0010013451648809304
122-123	0.029383835751316027	0.0	1.2271386824521206E-6	3.3132744426207256E-5	4.860738657477394	0.0010590206829561798
124-125	0.03220748185963836	0.0	1.2271386824521206E-6	3.4359883108659375E-5	4.931409574199812	0.001118536909055108
126-127	0.03539988314203754	0.0	1.2271386824521206E-6	3.4359883108659375E-5	5.002451700373671	0.0011737581497654533
128-129	0.03890765906582693	0.0	1.2271386824521206E-6	3.620059113233755E-5	5.073370499109944	0.0012216165583810862
130-131	0.04269092762382682	0.0	1.2271386824521206E-6	3.7427729814789676E-5	5.143951221139201	0.0012799056457975617
132-133	0.04706629059610985	0.0	1.2271386824521206E-6	3.86548684972418E-5	5.213983412177402	0.0013529203974034629
134-135	0.05176255033385412	0.0	1.2271386824521206E-6	3.926843783846786E-5	5.283465845085866	0.0014247080103269119
136-137	0.05695027911392046	0.0	1.2271386824521206E-6	3.926843783846786E-5	5.352821269140694	0.001496495623250361
138-139	0.06269144743977272	0.0	1.2271386824521206E-6	3.926843783846786E-5	5.422384693202199	0.0015879174550930439
>>END_MODULE
