##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065366_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81490382
Total Bases	12.2 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	16
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16391765104255	32.0	32.0	32.0	32.0	32.0
2	31.419341045670887	32.0	32.0	32.0	32.0	32.0
3	34.97384978511943	37.0	32.0	37.0	32.0	37.0
4	35.7490154359566	37.0	37.0	37.0	32.0	37.0
5	36.11565744040812	37.0	37.0	37.0	37.0	37.0
6	39.60132927098071	41.0	41.0	41.0	37.0	41.0
7	39.80935923211159	41.0	41.0	41.0	37.0	41.0
8	39.86702018405068	41.0	41.0	41.0	37.0	41.0
9	39.9163445938933	41.0	41.0	41.0	37.0	41.0
10-14	39.955858351480046	41.0	41.0	41.0	37.0	41.0
15-19	39.938609290112296	41.0	41.0	41.0	37.0	41.0
20-24	39.89778453609409	41.0	41.0	41.0	37.0	41.0
25-29	39.766715426613175	41.0	41.0	41.0	37.0	41.0
30-34	39.66204464963731	41.0	41.0	41.0	37.0	41.0
35-39	39.61120269628874	41.0	41.0	41.0	37.0	41.0
40-44	39.526799695208204	41.0	41.0	41.0	37.0	41.0
45-49	39.4768741690277	41.0	41.0	41.0	37.0	41.0
50-54	39.39301957131579	41.0	41.0	41.0	37.0	41.0
55-59	39.311407954867605	41.0	41.0	41.0	37.0	41.0
60-64	39.207476779284214	41.0	41.0	41.0	37.0	41.0
65-69	39.08682908616136	41.0	41.0	41.0	37.0	41.0
70-74	38.99789525590885	41.0	41.0	41.0	37.0	41.0
75-79	38.82124654907128	41.0	40.2	41.0	36.0	41.0
80-84	39.064648969248914	41.0	41.0	41.0	37.0	41.0
85-89	38.96987095237816	41.0	41.0	41.0	37.0	41.0
90-94	38.855509046454095	41.0	41.0	41.0	37.0	41.0
95-99	38.71782079509702	41.0	41.0	41.0	33.0	41.0
100-104	38.5765005691101	41.0	41.0	41.0	32.0	41.0
105-109	38.43863302788297	41.0	41.0	41.0	32.0	41.0
110-114	38.27809233978066	41.0	37.8	41.0	32.0	41.0
115-119	38.11274100543547	41.0	37.0	41.0	32.0	41.0
120-124	37.85776617908111	41.0	37.0	41.0	32.0	41.0
125-129	37.66474552052044	41.0	37.0	41.0	29.0	41.0
130-134	37.41764282071963	41.0	37.0	41.0	27.0	41.0
135-139	37.12371014778161	41.0	37.0	41.0	27.0	41.0
140-144	36.85597662801483	41.0	37.0	41.0	27.0	41.0
145-149	36.58037947349419	41.0	37.0	41.0	25.0	41.0
150	36.40308469777452	41.0	37.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	13.0
13	15.0
14	28.0
15	87.0
16	341.0
17	1322.0
18	2851.0
19	5365.0
20	9253.0
21	15540.0
22	26293.0
23	45490.0
24	75683.0
25	123778.0
26	194101.0
27	290828.0
28	420590.0
29	586128.0
30	791215.0
31	1045131.0
32	1350382.0
33	1723059.0
34	2198975.0
35	2824987.0
36	3740703.0
37	5247689.0
38	8304530.0
39	1.6908991E7
40	3.5557014E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.860090065215715	42.18122446629569	41.467172325973124	0.49151314251547684
2	16.889661940370946	41.34356991154809	41.49375986860634	0.2730082794746364
3	14.532335148031999	37.03126525882164	48.10494071241798	0.3314588807283802
4	15.320928332331189	36.11676273004111	48.21353909105686	0.34876984657084403
5	16.05439891053646	36.12577984970054	47.4787539957783	0.341067243984695
6	16.040807160046654	35.8214431564235	47.786722658929236	0.35102702460060675
7	16.473837013635688	36.28791234399592	46.92119980431824	0.31705083805015016
8	15.284830298649418	35.39285902081799	48.997059959174315	0.3252507213582857
9	15.737835928373812	34.884763086568476	49.054897376619934	0.322503608437775
10-14	15.817241314568047	35.80716304625133	48.07379121339636	0.301804425784257
15-19	15.80827005573926	35.654556980758656	48.240606286846486	0.2965666766556052
20-24	15.956350330460554	35.568196820837166	48.1782803809303	0.2971724677719765
25-29	16.09493068842837	35.46524878063664	48.135633582573675	0.3041869483613156
30-34	16.215335445995667	35.34893970826945	48.13089199173012	0.3048328540047652
35-39	16.26975223617564	35.30601566534698	48.11775125718441	0.306480841292965
40-44	16.299465157327003	35.28223339837888	48.10937863047425	0.3089228138198703
45-49	16.318036189261406	35.26363415667984	48.110139511928764	0.308190142129986
50-54	16.33806301583063	35.23886005665584	48.112903342147874	0.31017358536565975
55-59	16.349091740727552	35.255070668419634	48.083347247696636	0.31249034315616575
60-64	16.361661463122186	35.2356366965503	48.0874772527979	0.31522458752960797
65-69	16.387420238540333	35.229987031847806	48.064386753934144	0.3182059756777248
70-74	16.393365968621808	35.227799847493344	48.05691202958806	0.3219221542967902
75-79	16.412483462831403	35.21385263679568	48.058492732839575	0.31517116753334323
80-84	16.420271012406783	35.189509273731495	48.073665000618035	0.31655471324368484
85-89	16.44829352095677	35.18393275830933	48.03535000079605	0.33242371993783865
90-94	16.439508488285615	35.181902605460614	48.049404974724055	0.3291839315297187
95-99	16.462685299562025	35.16311720714853	48.03761133956264	0.33658615372680584
100-104	16.450436168783238	35.16680785318565	48.043945884023344	0.33881009400776435
105-109	16.471977030576955	35.14416421850254	48.03553039677203	0.34832835414847146
110-114	16.481754044495354	35.140289029786395	48.031025425613585	0.34693150010466794
115-119	16.472643900543403	35.13256981707539	48.03927238040195	0.3555139019792602
120-124	16.48608520558789	35.121773079128836	48.02289945063482	0.36924226464845383
125-129	16.49534078548611	35.129628684082675	47.99799400132433	0.3770365291068853
130-134	16.499387116420333	35.1208252763891	47.98868439779995	0.3911032093906179
135-139	16.516134940039713	35.09389724797658	47.98250066146946	0.40746715051424776
140-144	16.516398863824765	35.09186451063594	47.976036677633424	0.4156999479058636
145-149	16.51295622975447	35.07414516477067	47.98578087822512	0.42711772724973807
150	16.504933518000577	35.096079352164836	47.970435683743446	0.42855144609114443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5722.0
1	8135.0
2	15614.0
3	33772.5
4	77156.5
5	172526.5
6	361330.5
7	692678.0
8	1210574.0
9	1933610.0
10	2838294.5
11	3844894.0
12	4838377.5
13	5693842.0
14	6305722.5
15	6621424.5
16	6625613.0
17	6345770.5
18	5840387.0
19	5182052.0
20	4457305.0
21	3733619.5
22	3056398.0
23	2458804.5
24	1954478.0
25	1543900.0
26	1213744.5
27	948065.0
28	739896.0
29	579922.5
30	456900.0
31	359787.0
32	280114.5
33	216386.0
34	167292.5
35	130220.5
36	102698.0
37	81211.0
38	64625.5
39	52054.0
40	41873.0
41	33386.5
42	26676.5
43	21528.0
44	17462.0
45	14087.5
46	11428.0
47	9431.0
48	7736.0
49	7140.5
50	7532.0
51	7320.0
52	6280.0
53	5033.5
54	4076.5
55	3418.0
56	2959.5
57	2646.5
58	2518.5
59	2455.5
60	2154.0
61	1798.5
62	1447.0
63	1073.0
64	820.5
65	628.5
66	514.5
67	443.0
68	361.5
69	290.0
70	237.5
71	197.0
72	175.0
73	146.5
74	124.0
75	102.0
76	97.5
77	106.5
78	130.0
79	136.0
80	124.0
81	112.5
82	86.5
83	66.0
84	59.0
85	60.5
86	54.5
87	41.5
88	29.0
89	27.0
90	20.0
91	12.0
92	8.5
93	7.5
94	6.5
95	9.5
96	13.0
97	14.0
98	19.5
99	60.5
100	784.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7642104316065177
2	0.0011191504783963338
3	7.804602020395487E-4
4	0.00840221855874967
5	0.0
6	9.203540118390903E-5
7	0.0011375575586331159
8	2.0861357601686048E-5
9	1.6075516740122778E-4
10-14	2.3561062703080713E-4
15-19	2.9696756115341316E-4
20-24	3.21755762538946E-4
25-29	0.0014951457706996636
30-34	3.450713975055363E-4
35-39	9.642855766708763E-4
40-44	6.280495776789953E-4
45-49	0.001164309181910572
50-54	6.486655075441909E-4
55-59	3.0408496551163547E-4
60-64	9.812200904887156E-4
65-69	0.0011049156696798894
70-74	5.311056217652778E-4
75-79	3.8483069081698496E-4
80-84	0.0017231481378992676
85-89	0.0010923988551188777
90-94	6.125876302800986E-4
95-99	0.0018019304413126936
100-104	0.0029844012757235575
105-109	2.4886372480129005E-4
110-114	3.553793624381341E-4
115-119	5.649746494009563E-4
120-124	3.0972980345091524E-4
125-129	0.0014671670087397551
130-134	0.001035214192516609
135-139	0.0016676814694524316
140-144	0.001203086764276059
145-149	0.001800703302630242
150	0.005787186026444201
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	8.1490382E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.91732961230558
#Duplication Level	Percentage of total
1	74.9605889452088
2	15.845779814013216
3	3.7673556518523768
4	1.3784452821706468
5	0.6731689578144578
6	0.4571079751454648
7	0.344070275291938
8	0.28608619735055363
9	0.2240040392112077
>10	1.7816079626860957
>50	0.10883398678162633
>100	0.12398472826884047
>500	0.019069202179778772
>1k	0.02275708698890578
>5k	0.007139895036071918
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.3132744426207256E-5	0.0	0.0	0.0	0.0024984543574725175	8.786312966357183E-4
2	3.5587021791111494E-5	0.0	0.0	0.0	0.009683351343229684	0.0036666903831669363
3	3.5587021791111494E-5	0.0	0.0	0.0	0.016085333849582398	0.005313510495017682
4	3.804129915601574E-5	0.0	0.0	0.0	0.02277078539158155	0.007367740649442532
5	4.1722715203372096E-5	0.0	0.0	0.0	0.029589995049967982	0.009691941314006847
6	4.294985388582422E-5	0.0	0.0	0.0	0.03643129320463855	0.012305746707629866
7	4.540413125072846E-5	0.0	0.0	0.0	0.0434321193880279	0.015026313166626216
8	5.399410202789331E-5	0.0	0.0	0.0	0.0497985148725895	0.017712519742513907
9	5.7675518075249665E-5	1.2271386824521206E-6	0.0	0.0	0.056501146356142985	0.020736189456075933
10-11	6.197050346383208E-5	1.2271386824521206E-6	0.0	0.0	0.06608387232741159	0.025409747128194833
12-13	6.994690489977088E-5	1.2271386824521206E-6	0.0	0.0	0.07817118834956499	0.03183565883885536
14-15	7.792330633570965E-5	1.2271386824521206E-6	0.0	0.0	0.08967684063623607	0.03878126378153436
16-17	8.835398513655268E-5	1.2271386824521206E-6	0.0	0.0	0.10100333067526913	0.046071081124641186
18-19	1.0001180261984782E-4	1.2271386824521206E-6	0.0	0.0	0.11213225138642742	0.05331794861386219
20-21	1.1228318944436904E-4	1.2271386824521206E-6	0.0	0.0	0.12264944346438332	0.060374609607303106
22-23	1.282359923162466E-4	1.2271386824521206E-6	0.0	0.0	0.1328365352367596	0.06755275733030679
24-25	1.5155162728283688E-4	1.2271386824521206E-6	0.0	0.0	0.14305860046158575	0.07480085195821023
26-27	1.7241298488452294E-4	1.2271386824521206E-6	0.0	0.0	0.15306898426368892	0.08223179024979904
28-29	1.871386490739484E-4	1.2271386824521206E-6	0.0	0.0	0.16291493148234354	0.08970445125659124
30-31	2.0186431326337385E-4	1.2271386824521206E-6	0.0	0.0	0.17276946867177528	0.09701819780400588
32-33	2.478820138553284E-4	1.2271386824521206E-6	0.0	0.0	0.18269517990478926	0.10440864051907378
34-35	2.9267257576483073E-4	1.2271386824521206E-6	0.0	1.2271386824521206E-6	0.19262764040055672	0.11194020418262367
36-37	3.227374734849077E-4	1.2271386824521206E-6	0.0	1.2271386824521206E-6	0.2027036761221711	0.11940243451061501
38-39	3.644601886882798E-4	1.2271386824521206E-6	0.0	1.2271386824521206E-6	0.21274473839133556	0.12687693622543086
40-41	4.055693345504258E-4	1.2271386824521206E-6	0.0	1.2271386824521206E-6	0.22272751157308357	0.13460606921685556
42-43	4.5220060448360644E-4	1.2271386824521206E-6	0.0	1.2271386824521206E-6	0.23281152369613387	0.1423462464564223
44-45	4.969911663931088E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.24281577180482478	0.15018643549860888
46-47	5.331917575254464E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.253197978627711	0.15806712011731644
48-49	5.779823194349488E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.2633341441447654	0.16593860119590553
50-51	6.356578375101984E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.27371757810633407	0.17400212947829843
52-53	6.82289107443379E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.28411819200945704	0.18199080230106174
54-55	7.546902897080541E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.2941310300889251	0.19024085566318735
56-57	8.295457493376335E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.3044788770287026	0.19860626006146345
58-59	8.872212674128832E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.3148831723478729	0.2068716526571197
60-61	9.534867562652977E-4	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.32544638212642074	0.21515483876367153
62-63	0.001028955785236103	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.33565678953376366	0.22352699242470112
64-65	0.0011136283543252993	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.34618318515183794	0.2320494705743311
66-67	0.0012185487116749558	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.356760507025234	0.2405940372202452
68-69	0.0013504661200385588	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.36740716223418857	0.24930549472697283
70-71	0.0014719528496013184	1.2271386824521206E-6	0.0	2.454277364904241E-6	0.3782004605156962	0.25802124721908903
72-73	0.0016008024112587913	1.2271386824521206E-6	0.0	3.6814160473563615E-6	0.38910542841730694	0.26689223275453533
74-75	0.0017732153961433142	1.2271386824521206E-6	0.0	3.6814160473563615E-6	0.40002757626047203	0.2757797846621949
76-77	0.001954831921146228	1.2271386824521206E-6	0.0	3.6814160473563615E-6	0.4109368391474714	0.28472194424122343
78-79	0.002160991219798184	1.2271386824521206E-6	0.0	4.294985388582422E-6	0.42185223772788305	0.29354691207607786
80-81	0.002392306861440409	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.432752297074764	0.3021884226779057
82-83	0.002675775897086849	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.44364315288152656	0.3110704524614942
84-85	0.0029985133705717564	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.4543928877398071	0.3199414379969405
86-87	0.0033390443549522197	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.4651285105032395	0.3286749839999523
88-89	0.0037047316823229518	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.47601077633922495	0.3376263471190993
90-91	0.004102938184778665	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.48707159085350715	0.34650530905598165
92-93	0.004649628467811084	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.49827291274693003	0.35556097896313704
94-95	0.005160731729052393	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5095147302168739	0.3646939586072869
96-97	0.005787186026444201	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5208007246793861	0.37353978779974306
98-99	0.006436955958802598	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5320584949522018	0.38235248424777296
100-101	0.00722477899293686	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5435377637572002	0.39157504501574186
102-103	0.00809850173484277	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5549550620587347	0.40079699221436954
104-105	0.009038489965601092	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5662889149298626	0.4099698538656992
106-107	0.010090761385803788	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5776847383044541	0.4193678954652587
108-109	0.011265133104910466	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.5889971162486389	0.42864935888998534
110-111	0.012524177393106342	1.2271386824521206E-6	0.0	4.908554729808482E-6	0.600307039915459	0.437800745614372
112-113	0.01391391195098337	1.2271386824521206E-6	0.0	5.522124071034543E-6	0.611820668603566	0.4471337488637616
114-115	0.015519009347630742	1.2271386824521206E-6	0.0	6.135693412260603E-6	0.6231600435987648	0.45643852792345485
116-117	0.017300201145209998	1.2271386824521206E-6	0.0	6.135693412260603E-6	0.6346779672722604	0.46565495299801146
118-119	0.019270985869228104	1.2271386824521206E-6	0.0	6.135693412260603E-6	0.6462149115953341	0.47477197983928954
120-121	0.021365098030832644	1.2271386824521206E-6	0.0	6.135693412260603E-6	0.6577420388089481	0.48377610992178194
122-123	0.023763540585685312	1.2271386824521206E-6	0.0	6.135693412260603E-6	0.6694704167664842	0.4931637208425407
124-125	0.02641354657044067	1.2271386824521206E-6	0.0	6.135693412260603E-6	0.6811668891182765	0.502243319954004
126-127	0.02943782985334392	1.2271386824521206E-6	0.0	7.362832094712723E-6	0.6930222759294464	0.5113106476786426
128-129	0.03276398925213039	1.2271386824521206E-6	0.0	7.362832094712723E-6	0.7048641642151094	0.5204945535781142
130-131	0.03639386547482376	1.2271386824521206E-6	0.0	7.362832094712723E-6	0.7169392088504383	0.5298649845573187
132-133	0.040596815462222276	1.2271386824521206E-6	0.0	9.817109459616965E-6	0.7288387726541765	0.5391630143542584
134-135	0.04513170646322409	1.2271386824521206E-6	0.0	9.817109459616965E-6	0.740855528202089	0.5485518524136996
136-137	0.05015438509050062	1.2271386824521206E-6	0.0	9.817109459616965E-6	0.7528011097064191	0.5576118173062437
138-139	0.05576731742403662	1.2271386824521206E-6	0.0	9.817109459616965E-6	0.7647546676121852	0.5667527733518294
>>END_MODULE
