SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR10065366_1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.6.10
Cutadapt version: 4.2
Python version: could not detect
Number of cores used for trimming: 2
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Using Illumina adapter for trimming (count: 465). Second best hit was Nextera (count: 0)
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed


This is cutadapt 4.2 with Python 3.10.8
Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR10065366_1.fastq.gz
Processing single-end reads on 2 cores ...
Finished in 1517.310 s (18.619 µs/read; 3.22 M reads/minute).

=== Summary ===

Total reads processed:              81,490,382
Reads with adapters:                33,604,238 (41.2%)
Reads written (passing filters):    81,490,382 (100.0%)

Total basepairs processed: 12,223,557,300 bp
Quality-trimmed:              24,238,565 bp (0.2%)
Total written (filtered):  12,154,834,160 bp (99.4%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 33604238 times

Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 41.6%
  C: 0.1%
  G: 12.5%
  T: 45.7%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	27039691	20372595.5	0	27039691
2	4301691	5093148.9	0	4301691
3	1567869	1273287.2	0	1567869
4	618878	318321.8	0	618878
5	4753	79580.5	0	4753
6	4621	19895.1	0	4621
7	3577	4973.8	0	3577
8	3191	1243.4	0	3191
9	3711	310.9	0	2821 890
10	4457	77.7	1	2885 1572
11	2939	19.4	1	2516 423
12	3461	4.9	1	3144 317
13	2123	1.2	1	2022 101
14	2380	1.2	1	2265 115
15	1940	1.2	1	1850 90
16	1990	1.2	1	1899 91
17	1786	1.2	1	1692 94
18	2015	1.2	1	1908 107
19	1656	1.2	1	1590 66
20	1570	1.2	1	1505 65
21	1401	1.2	1	1331 70
22	1503	1.2	1	1438 65
23	1350	1.2	1	1286 64
24	1563	1.2	1	1454 109
25	1243	1.2	1	1179 64
26	1171	1.2	1	1106 65
27	1078	1.2	1	1023 55
28	1001	1.2	1	939 62
29	1027	1.2	1	979 48
30	991	1.2	1	951 40
31	864	1.2	1	818 46
32	796	1.2	1	764 32
33	825	1.2	1	790 35
34	781	1.2	1	746 35
35	756	1.2	1	715 41
36	715	1.2	1	677 38
37	683	1.2	1	650 33
38	692	1.2	1	657 35
39	519	1.2	1	508 11
40	577	1.2	1	536 41
41	589	1.2	1	550 39
42	500	1.2	1	475 25
43	588	1.2	1	555 33
44	413	1.2	1	401 12
45	644	1.2	1	614 30
46	217	1.2	1	201 16
47	317	1.2	1	302 15
48	377	1.2	1	362 15
49	350	1.2	1	322 28
50	375	1.2	1	360 15
51	328	1.2	1	319 9
52	318	1.2	1	292 26
53	273	1.2	1	256 17
54	279	1.2	1	259 20
55	309	1.2	1	290 19
56	210	1.2	1	193 17
57	197	1.2	1	183 14
58	237	1.2	1	226 11
59	213	1.2	1	203 10
60	187	1.2	1	177 10
61	161	1.2	1	153 8
62	173	1.2	1	157 16
63	177	1.2	1	164 13
64	163	1.2	1	155 8
65	108	1.2	1	101 7
66	159	1.2	1	143 16
67	133	1.2	1	123 10
68	153	1.2	1	141 12
69	146	1.2	1	130 16
70	162	1.2	1	144 18
71	239	1.2	1	179 60
72	816	1.2	1	784 32
73	171	1.2	1	163 8
74	51	1.2	1	45 6
75	36	1.2	1	24 12
76	25	1.2	1	19 6
77	35	1.2	1	28 7
78	26	1.2	1	23 3
79	23	1.2	1	20 3
80	26	1.2	1	20 6
81	24	1.2	1	18 6
82	17	1.2	1	12 5
83	19	1.2	1	15 4
84	14	1.2	1	12 2
85	17	1.2	1	15 2
86	13	1.2	1	10 3
87	11	1.2	1	9 2
88	13	1.2	1	9 4
89	16	1.2	1	8 8
90	14	1.2	1	10 4
91	16	1.2	1	14 2
92	10	1.2	1	9 1
93	10	1.2	1	3 7
94	13	1.2	1	7 6
95	11	1.2	1	10 1
96	10	1.2	1	7 3
97	4	1.2	1	4
98	7	1.2	1	4 3
99	6	1.2	1	4 2
100	16	1.2	1	11 5
101	9	1.2	1	7 2
102	11	1.2	1	6 5
103	8	1.2	1	4 4
104	8	1.2	1	3 5
105	4	1.2	1	4
106	8	1.2	1	7 1
107	10	1.2	1	7 3
108	7	1.2	1	4 3
109	8	1.2	1	6 2
110	5	1.2	1	4 1
111	5	1.2	1	3 2
112	6	1.2	1	6
113	5	1.2	1	3 2
114	3	1.2	1	3
115	4	1.2	1	2 2
116	6	1.2	1	4 2
117	5	1.2	1	4 1
118	6	1.2	1	5 1
119	6	1.2	1	3 3
120	6	1.2	1	2 4
121	7	1.2	1	5 2
123	4	1.2	1	3 1
124	3	1.2	1	1 2
125	2	1.2	1	1 1
126	5	1.2	1	2 3
127	4	1.2	1	4
128	6	1.2	1	5 1
130	1	1.2	1	0 1
131	2	1.2	1	1 1
132	3	1.2	1	3
133	2	1.2	1	1 1
134	3	1.2	1	1 2
136	5	1.2	1	1 4
138	2	1.2	1	1 1
139	1	1.2	1	0 1
140	5	1.2	1	2 3
141	1	1.2	1	1
142	3	1.2	1	0 3
143	2	1.2	1	2
145	1	1.2	1	0 1
146	5	1.2	1	0 5
147	1	1.2	1	0 1
148	1	1.2	1	0 1
150	39	1.2	1	4 35

RUN STATISTICS FOR INPUT FILE: SRR10065366_1.fastq.gz
=============================================
81490382 sequences processed in total