##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR10065365_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70095226
Total Bases	10.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86833719603101	32.0	32.0	32.0	32.0	32.0
2	31.06330839135892	32.0	32.0	32.0	32.0	32.0
3	34.915509538409935	37.0	32.0	37.0	32.0	37.0
4	35.65647205417385	37.0	37.0	37.0	32.0	37.0
5	35.944541429968424	37.0	37.0	37.0	37.0	37.0
6	39.24773591570987	41.0	41.0	41.0	37.0	41.0
7	39.433946015096666	41.0	41.0	41.0	37.0	41.0
8	39.59589236505208	41.0	41.0	41.0	37.0	41.0
9	39.61844064815484	41.0	41.0	41.0	37.0	41.0
10-14	39.65155457234705	41.0	41.0	41.0	37.0	41.0
15-19	39.58999042531085	41.0	41.0	41.0	37.0	41.0
20-24	39.519849448805545	41.0	41.0	41.0	37.0	41.0
25-29	39.45596120911287	41.0	41.0	41.0	37.0	41.0
30-34	39.389040443353444	41.0	41.0	41.0	37.0	41.0
35-39	39.30775995500749	41.0	41.0	41.0	37.0	41.0
40-44	39.23839195268448	41.0	41.0	41.0	37.0	41.0
45-49	39.14470014548495	41.0	41.0	41.0	37.0	41.0
50-54	39.0165472296216	41.0	41.0	41.0	37.0	41.0
55-59	38.90051595239881	41.0	41.0	41.0	37.0	41.0
60-64	38.795991441699606	41.0	41.0	41.0	37.0	41.0
65-69	38.69714651037718	41.0	41.0	41.0	33.0	41.0
70-74	38.577428945018305	41.0	41.0	41.0	32.0	41.0
75-79	38.35550823960536	41.0	39.4	41.0	32.0	41.0
80-84	38.53442514330434	41.0	41.0	41.0	32.0	41.0
85-89	38.40868609796621	41.0	41.0	41.0	32.0	41.0
90-94	38.23323493100658	41.0	41.0	41.0	32.0	41.0
95-99	38.03903624192609	41.0	39.4	41.0	32.0	41.0
100-104	37.82898123760954	41.0	37.0	41.0	29.0	41.0
105-109	37.58636549656035	41.0	37.0	41.0	27.0	41.0
110-114	37.379433141423924	41.0	37.0	41.0	27.0	41.0
115-119	37.11831377503512	41.0	37.0	41.0	27.0	41.0
120-124	36.77431705834004	41.0	37.0	41.0	26.0	41.0
125-129	36.40893036852467	41.0	37.0	41.0	22.0	41.0
130-134	36.046252613551744	41.0	37.0	41.0	22.0	41.0
135-139	35.60088951849588	41.0	33.0	41.0	22.0	41.0
140-144	35.03028956922116	39.4	32.0	41.0	22.0	41.0
145-149	34.45270282172997	37.0	32.0	41.0	16.0	41.0
150	34.07356398280248	37.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	66.0
12	652.0
13	1790.0
14	5092.0
15	12000.0
16	21160.0
17	30616.0
18	44605.0
19	67330.0
20	106518.0
21	166895.0
22	241961.0
23	321373.0
24	404121.0
25	491984.0
26	587006.0
27	695070.0
28	815468.0
29	939487.0
30	1068379.0
31	1190681.0
32	1309014.0
33	1457075.0
34	1692395.0
35	2103721.0
36	2814504.0
37	4065882.0
38	6553612.0
39	1.3110952E7
40	2.9775817E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.12527389036372116	50.282646487733196	32.97111637725935	16.620963244643736
2	0.1411604190533552	52.40831014305992	34.48113666109655	12.96939277679017
3	0.19611248650311588	49.08126315484319	35.07983627004873	15.64278808860496
4	0.23911739988389094	47.73529751768182	35.1337957066751	16.89178937575918
5	0.2523443762810011	47.919535240439046	35.02419991427929	16.80392046900066
6	0.2760554243383642	47.41230977133284	35.22920513768019	17.082429666648604
7	0.27902484381525056	48.22966602331299	34.83191257968164	16.659396553190124
8	0.25206642007091606	46.84311725040312	36.02217686886359	16.882639460662375
9	0.2650274993315107	47.37244358564331	35.76947346866727	16.59305544635791
10-14	0.26276763968576466	47.72159354678569	35.573312674220176	16.44232613930837
15-19	0.2778634697700821	47.343236977173184	35.78012084118345	16.59877871187328
20-24	0.3042907808007294	47.53082833776992	35.378235912333125	16.78664496909622
25-29	0.3102621917988671	47.45954769271712	35.33581384368927	16.89437627179474
30-34	0.30066014781155226	47.62310511405638	35.16210784613934	16.91412689199273
35-39	0.3090686102789197	47.53709730520062	35.216398087790814	16.93743599672964
40-44	0.30505228242598004	47.607847794796456	35.14842456878706	16.93867535399051
45-49	0.30746659234858015	47.54436412419443	35.205499043834664	16.94267023962233
50-54	0.3099516282301584	47.58757011464185	35.17413500451167	16.928343252616322
55-59	0.31649370433952095	47.56085795048204	35.18874632467645	16.933902020501996
60-64	0.3207911575039955	47.5485909619953	35.17730034951821	16.95331753098249
65-69	0.32665479551133136	47.526521063824894	35.19019899793114	16.956625142732637
70-74	0.33613450509349163	47.49328782013799	35.20128567382917	16.96929200093935
75-79	0.34238432057187435	47.48696511923033	35.20287095184058	16.967779608357212
80-84	0.34716326743914855	47.465124139574186	35.21475233728721	16.97296025569946
85-89	0.35506164235417215	47.44400155110364	35.21324923025684	16.987687576285342
90-94	0.36752052821657594	47.41055787168122	35.21916448210598	17.002757117996225
95-99	0.37794564031085986	47.39635274534958	35.202454170725304	17.02324744361425
100-104	0.3866018808874566	47.355145266468504	35.205676842913405	17.052576009730632
105-109	0.3976271478854375	47.33125727300968	35.193188907683115	17.07792667142177
110-114	0.40152911935889557	47.30495519182623	35.20853370578281	17.084981983032062
115-119	0.427123678692763	47.285399213092845	35.17295584303235	17.11452126518204
120-124	0.43718788239345197	47.245493157627344	35.178400447501055	17.138918512478153
125-129	0.45851862044187525	47.23161716344946	35.14543092816614	17.164433287942522
130-134	0.46981497043266546	47.19215350943705	35.146843389196775	17.191188130933515
135-139	0.4983844292197985	47.16368378528413	35.11194078027126	17.225991005224813
140-144	0.5283693311948544	47.10372877254431	35.10354874773603	17.264353148524812
145-149	0.5463090648264811	47.0553823022563	35.07046435047872	17.32784428243849
150	0.5640451966676785	47.0123480901211	35.05526993164535	17.368336781565866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4549.0
1	5963.0
2	11000.0
3	24730.5
4	57336.0
5	129844.0
6	273317.0
7	523887.0
8	919918.0
9	1480538.0
10	2191874.0
11	3001206.0
12	3822752.5
13	4557688.0
14	5120543.5
15	5457273.0
16	5540811.0
17	5387952.0
18	5034720.0
19	4535152.5
20	3964548.0
21	3378381.5
22	2816367.5
23	2309493.5
24	1873444.5
25	1506911.5
26	1204273.5
27	955654.0
28	756492.5
29	602216.5
30	481398.0
31	385751.0
32	306517.0
33	241421.0
34	191246.5
35	153017.0
36	123298.5
37	100423.0
38	82799.5
39	68704.5
40	57309.0
41	47927.5
42	40644.5
43	34928.0
44	30244.5
45	26338.0
46	22918.5
47	20114.5
48	17963.5
49	17474.5
50	17120.0
51	16305.5
52	14956.0
53	12914.5
54	11576.5
55	10435.5
56	9432.5
57	8525.5
58	7810.5
59	7238.5
60	6619.0
61	6031.0
62	5529.0
63	5051.0
64	4578.0
65	4131.5
66	3823.0
67	3521.0
68	3135.0
69	2759.0
70	2515.0
71	2303.5
72	2092.5
73	1953.5
74	1794.5
75	1627.0
76	1492.5
77	1348.5
78	1242.5
79	1157.0
80	1070.0
81	998.5
82	962.0
83	885.5
84	801.5
85	722.0
86	679.5
87	639.5
88	595.5
89	598.5
90	578.0
91	585.5
92	590.0
93	557.0
94	550.0
95	554.0
96	579.0
97	629.5
98	716.5
99	1108.5
100	2762.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9429529480367179
2	0.004764946474386144
3	0.001811820964811498
4	0.0011227583459107473
5	2.040081873764128E-4
6	3.4809788615276023E-4
7	4.850544315243381E-5
8	2.981658123193725E-4
9	8.745246074247625E-4
10-14	6.357066314330736E-4
15-19	7.327175177379412E-4
20-24	7.081794700255335E-4
25-29	2.6877722029172143E-4
30-34	1.9459242488211682E-4
35-39	2.4966036916693874E-4
40-44	0.0026595249154343263
45-49	1.8688861920496556E-4
50-54	0.002068614487383206
55-59	4.4054355427857524E-4
60-64	4.833424747071933E-4
65-69	0.0037634517363564816
70-74	0.0023941716087768944
75-79	0.0031097695583433885
80-84	4.425408372319108E-4
85-89	0.002354796601982566
90-94	8.325816654047168E-4
95-99	7.740898074856054E-4
100-104	5.395517235367783E-4
105-109	0.0026004624052428336
110-114	1.9316579420116287E-4
115-119	0.003108913579934816
120-124	5.33274548540581E-4
125-129	0.0026954760085943653
130-134	6.2714684734735E-4
135-139	0.0020300954589974503
140-144	0.0038008294601974747
145-149	0.0012465898890175488
150	0.011572828083898324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	7.0095226E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.60043277332574
#Duplication Level	Percentage of total
1	75.98099065467869
2	15.376690854345403
3	3.6321812733709553
4	1.2894505461605514
5	0.603160353629978
6	0.38905893809636094
7	0.35324304328970996
8	0.2802091723138005
9	0.23520204765661523
>10	1.534639381226487
>50	0.11807360595677856
>100	0.13801951630946827
>500	0.023105764198322908
>1k	0.0377827677742402
>5k	0.00819208099269137
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2839676128585418E-5	0.0	0.0	0.0	0.16523521872944671	4.279892042861806E-6
2	1.2839676128585418E-5	0.0	0.0	0.0	0.2125893709223507	7.133153404769677E-6
3	1.2839676128585418E-5	0.0	0.0	0.0	0.24810106183265604	9.986414766677549E-6
4	1.2839676128585418E-5	0.0	0.0	0.0	0.28471696489001974	1.4266306809539355E-5
5	1.2839676128585418E-5	0.0	0.0	0.0	0.3235669715937573	2.139946021430903E-5
6	1.2839676128585418E-5	0.0	0.0	0.0	0.36241840492817584	2.139946021430903E-5
7	1.2839676128585418E-5	0.0	0.0	0.0	0.40165217528509	2.2826090895262967E-5
8	1.4266306809539355E-5	0.0	0.0	0.0	0.43933947798385015	2.4252721576216903E-5
9	1.569293749049329E-5	0.0	0.0	0.0	0.4784919303919499	2.5679352257170836E-5
10-11	1.7119568171447224E-5	0.0	0.0	0.0	0.535906682146941	3.0672559640509614E-5
12-13	2.1399460214309034E-5	0.0	0.0	0.0	0.6096798946050905	3.423913634289445E-5
14-15	3.209919032146355E-5	0.0	0.0	0.0	0.6822411842997695	3.637908236432535E-5
16-17	4.7078812471479863E-5	0.0	0.0	0.0	0.7550892267613204	3.9232343726233226E-5
18-19	7.989131813342039E-5	0.0	0.0	0.0	0.8280863521290309	4.5652181790525934E-5
20-21	1.3125002264776208E-4	0.0	0.0	0.0	0.9015806582890538	4.850544315243381E-5
22-23	2.0472150271688973E-4	0.0	0.0	0.0	0.9745114453300999	5.207201985481864E-5
24-25	2.8175955948840225E-4	0.0	0.0	0.0	1.0471754524338077	5.4925281216726514E-5
26-27	4.0088322134805587E-4	0.0	0.0	0.0	1.1201233590430253	6.063180394054225E-5
28-29	5.171536218458016E-4	0.0	0.0	0.0	1.1938544858960864	6.41983806429271E-5
30-31	6.248642382578238E-4	0.0	0.0	0.0	1.2668730107240114	6.41983806429271E-5
32-33	7.753737750984639E-4	0.0	0.0	0.0	1.340112663307484	6.776495734531194E-5
34-35	9.458561414724591E-4	0.0	0.0	0.0	1.413004218004804	7.133153404769677E-5
36-37	0.001106352093079777	0.0	0.0	0.0	1.4866197877727079	7.703805677151252E-5
38-39	0.0012996605503490352	0.0	0.0	0.0	1.5601811455747359	7.846468745246645E-5
40-41	0.0014943956382992474	0.0	0.0	0.0	1.6340121365754638	8.559784085723612E-5
42-43	0.001701257087037568	0.0	0.0	0.0	1.7073395269458151	9.059104824057489E-5
44-45	0.0019131117431592274	0.0	0.0	0.0	1.7805128412026234	1.0200409368820638E-4
46-47	0.0021485058055166267	0.0	0.0	0.0	1.853522092931122	1.0985056243345303E-4
48-49	0.0024174256888764437	0.0	0.0	0.0	1.9265242115062158	1.255434999239463E-4
50-51	0.002669226004064813	0.0	0.0	7.133153404769677E-7	1.9996804061948525	1.3624323003110083E-4
52-53	0.0029046200664222125	0.0	0.0	1.4266306809539354E-6	2.0727795356562515	1.4408969877634746E-4
54-55	0.0031749665804629837	0.0	0.0	1.4266306809539354E-6	2.1455084544559426	1.54789428883502E-4
56-57	0.0034424598331418465	0.0	0.0	1.4266306809539354E-6	2.2182544928238053	1.633492129692256E-4
58-59	0.0036864136795849694	0.0	0.0	2.853261361907871E-6	2.290648153413472	1.8118209648114978E-4
60-61	0.003911108011835213	0.0	0.0	4.279892042861806E-6	2.363212296369513	1.9330845726925827E-4
62-63	0.0041814545258759845	0.0	0.0	4.279892042861806E-6	2.4358370711294945	2.075747640787976E-4
64-65	0.004504586375112051	0.0	0.0	4.279892042861806E-6	2.5076650726541634	2.361073776978763E-4
66-67	0.004831284801050502	0.0	0.0	4.279892042861806E-6	2.579097897480208	2.610734146145702E-4
68-69	0.005126597352007967	0.0	0.0	4.279892042861806E-6	2.6509380253656647	2.8603945153126407E-4
70-71	0.005467562084755958	0.0	0.0	4.279892042861806E-6	2.7228744508220863	3.110054884479579E-4
72-73	0.005823506439653964	0.0	0.0	5.706522723815742E-6	2.7947680773580785	3.395381020670366E-4
74-75	0.006224389661002021	0.0	0.0	5.706522723815742E-6	2.86635997150505	3.687840310265923E-4
76-77	0.006646672342564385	0.0	0.0	5.706522723815742E-6	2.937646566686296	4.130095821361643E-4
78-79	0.007121027043981569	0.0	0.0	5.706522723815742E-6	3.0090701184129145	4.4082888041476604E-4
80-81	0.007592528484036845	0.0	0.0	5.706522723815742E-6	3.080256136131154	4.7720796277909137E-4
82-83	0.008052616878644489	0.0	0.0	5.706522723815742E-6	3.1515455845737623	5.143003604838937E-4
84-85	0.008551937616978367	0.0	0.0	5.706522723815742E-6	3.2232123768314835	5.549593348910808E-4
86-87	0.009146842610936156	0.0	0.0	6.419838064292709E-6	3.294340616007144	6.020381473625608E-4
88-89	0.009743174235574902	0.0	0.0	7.133153404769677E-6	3.364843277629207	6.391305450673631E-4
90-91	0.010383731411323219	0.0	0.0	7.133153404769677E-6	3.4349992679957975	6.84069411517412E-4
92-93	0.011074933976245399	0.0	0.0	7.133153404769677E-6	3.5059705777965533	7.268683319460301E-4
94-95	0.011856727589408159	0.0	0.0	7.133153404769677E-6	3.576424733975464	7.832202438437105E-4
96-97	0.012697726375830502	0.0	0.0	7.133153404769677E-6	3.647233407878591	8.531251472104534E-4
98-99	0.013570111037233833	0.0	0.0	7.133153404769677E-6	3.7181554989208534	9.173235278533804E-4
100-101	0.014588012028094467	0.0	0.0	7.133153404769677E-6	3.788943486679107	9.800952778153537E-4
102-103	0.015638012209276565	0.0	0.0	7.133153404769677E-6	3.8593027719177337	0.0010557067039059123
104-105	0.016787163222784958	0.0	0.0	7.133153404769677E-6	3.92945234244626	0.0011199050845488393
106-107	0.018027618599874402	0.0	0.0	7.133153404769677E-6	3.9994357105004554	0.0011898099879155822
108-109	0.019495621570576004	0.0	0.0	7.133153404769677E-6	4.069083820344627	0.0012647080986656638
110-111	0.021135533538332554	0.0	0.0	7.846468745246644E-6	4.138672725015539	0.0013560124622467155
112-113	0.022958767548591684	0.0	0.0	9.986414766677549E-6	4.208004836163878	0.0014430369337849057
114-115	0.024942497510458128	0.0	0.0	1.1413045447631483E-5	4.2773997190621795	0.0015478942888350199
116-117	0.027129522344360515	0.0	0.0	1.1413045447631483E-5	4.346858087025785	0.0016484717518422723
118-119	0.029592600215027486	0.0	0.0	1.1413045447631483E-5	4.416292202267812	0.0017461959534876169
120-121	0.0323081917162233	0.0	0.0	1.1413045447631483E-5	4.485378219623687	0.0018517666238782081
122-123	0.03532194902973849	0.0	0.0	1.1413045447631483E-5	4.554318720650105	0.0019794500698235855
124-125	0.038768688754923196	0.0	0.0	1.4266306809539355E-5	4.62389763890625	0.0021092734617903933
126-127	0.042567806258303524	0.0	0.0	1.569293749049329E-5	4.692987222838828	0.002256929737269126
128-129	0.0470688260567132	0.0	0.0	1.569293749049329E-5	4.762278675012761	0.0023945995979811805
130-131	0.05209698589173534	0.0	0.0	1.569293749049329E-5	4.831707797047406	0.002504450160414634
132-133	0.057553134931043665	0.0	0.0	1.569293749049329E-5	4.900836613323709	0.0026442599671481193
134-135	0.06358278950409547	0.0	0.0	1.6406252830970256E-5	4.968965361492664	0.002788349665924467
136-137	0.07034002572443379	0.0	0.0	1.7119568171447224E-5	5.037335210246701	0.0029452790408293996
138-139	0.07809875668280176	0.0	0.0	1.7119568171447224E-5	5.106039603895421	0.0031114815151605333
>>END_MODULE
